scholarly journals GENETIC PERFORMANCE OF THE STRIPED CATFISH (Pangasianodon hypophthalmus Sauvage, 1878) POPULATION DERIVED FROM SELECTIVE BREEDING

2019 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Huria Marnis ◽  
Evi Tahapari ◽  
Jadmiko Darmawan
Keyword(s):  
Microsatellite Loci ◽  
Genetic Improvement ◽  
Selective Breeding ◽  
Growth Trait ◽  
Performance Data ◽  
Gene Frequencies ◽  
Microsoft Excel ◽  
Pangasianodon Hypophthalmus ◽  
Striped Catfish ◽  
Nei's Distance

Selective breeding to improve growth trait in striped catfish (Pangasianodon hypophthalmus) need genetic performance data. This study was carried out to evaluate genetic performance of different generations of the striped catfish population derived from selective breeding. Fifty fish of each population from four generations selected striped catfish was analyzed using five microsatellite loci (Pg-1, Pg-2, Pg-3, Pg-13, and Pg-14). Microsatellite allele data were analyzed using Microsoft Excel, Arlequin, and Fstat software. A neighborhood joining dendrogram was constructed based on Nei’s distance (Da) matrix with 1,000 bootstrap replications using MEGA7 software. The result showed that totally 31.5 exist, ranged from 4-7. Number of allele was ranged from 5.0 to 5.2, and polymorphic data was from 0.45-0.60. There were some exception, such as the allele of the loci Pg-2—194 bp, Pg-13—227 bp, Pg-13—229 bp, and Pg-14—279 bp; their gene frequencies were increased by generation. Further analyses indi­cated that most genetic variations arise from individuals within populations (approximately 57.10%). The founder generation closely related to G-0 generation. Likewise, G-1 generation closely related to G-2 generation. This result indicated that selection activity had a very significant impact on the genetic improvement of the selected population.

scholarly journals GENETIC DIVERSITY ANALYSIS OF the first and second generations OF FAST-GROWING STRIPED CATFISH (Pangasianodon hypophthalmus Sauvage,1878) USING MICROSATELLITE ANALYSIS

2018 ◽  
Vol 13 (1) ◽  
pp. 1
Author(s):  
Huria Marnis ◽  
Evi Tahapari ◽  
Jadmiko Darmawan
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Selective Breeding ◽  
Microsatellite Analysis ◽  
Breeding Program ◽  
Diversity Analysis ◽  
Pangasianodon Hypophthalmus ◽  
Fast Growing ◽  
Striped Catfish ◽  
Inbreeding Level

Genetic diversity is an important aspect of a selective breeding program to produce fish broodstock carrying superior traits such as fast-growing, disease resistant, and other traits. We have carried out a breeding program to produce a fast-growing striped catfish (Pangasianodon hypophthalmus) since 2010. The aim of this study was to evaluate the genetic variation of the first (G-1) and second (G-2) generations of fast-growing striped catfish using microsatellite analysis. The G-1 and G-2 populations were selected individually from populations. DNA samples were collected from 40 ind. fish of each population and analyzed using five microsatellite loci (Pg1, Pg2, Pg3, Pg13, and Pg14). The results showed that the number of alleles per loci in the G-1 and G2 populations ranged from 4 to 7 alleles, with an average of five for each generation. The average of observed heterozygosity of the G-1 population (0.420) was lower than the G-2 population (0.495). Inbreeding level showed that the G-1 population was more inbred than the G-2 population. The study also found that both striped catfish populations had relatively low genetic variation. This result suggests that monitoring of genetic variation and better scheme of good spawning were needed on the next selection program to produce the intended fast-growing striped catfish.

scholarly journals Performance of the third generation striped catfish, Pangasianodon hypophthalmus Sauvage, 1878 as results of the selection for bodyweight character in Freshwater Aquaculture Fisheries Center, Sungai Gelam, Jambi

2019 ◽  
Vol 19 (3) ◽  
pp. 411
Author(s):  
Irwan Irwan ◽  
Dinar Tri Soelistyowati ◽  
Odang Carman ◽  
Ronny Rachman Noor
Keyword(s):  
Water Exchange ◽  
Selective Breeding ◽  
Selection Response ◽  
Growth Line ◽  
Third Generation ◽  
Base Population ◽  
Pangasianodon Hypophthalmus ◽  
The Third ◽  
Three Generations ◽  
Striped Catfish

Selective breeding of striped catfish (Pangasianodon hypophthalmus Sauvage, 1878) has been conducted at BPBAT Sungai Gelam to produce a growth line with mass selection method. Until 2018, the selective breeding program has produced three generations. Therefore, it was necessary to evaluate the performance of the third generation growth line (G3Ps) at the reproductive, seed and grow-out phase compared to the second generation the base population (G2Ds). A total of 10 pairs of broodstock (G3Ps and G2Ds) were spawned then the seeds were raised for 120 days in the media with regular water exchange (first treatment: T1) and without water exchange (second treatment: T2). The results showed that the number of eggs per gram of G3Ps (1600±124 eggs g-1) was significantly different from G2Ds (1490±101 eggs g-1). The bodyweight of G3Ps seeds at aged 40 days larger than G2Ds with selection response is 32.25%, while survival and feed efficiency were not significantly different. At the grow-out phase, between treat-ments were not significantly different and there was no interaction between lines and treatments for all the characters measured (p>0.05). The bodyweight of G3Ps was larger than the G2Ds with response selection 18.41% in T1 and 42.6% in T2. The control used was the base population so that the selection response obtained was an accumulation of three generations. Thus the selection response per generation was 6.14% in T1 and 14.20% in T2 measured at 162 days from hatching. It can be concluded that there is an improvement in the character of bodyweight for the third generation of growth line (G3Ps) as results of the selection at BPBAT Sungai Gelam both in good (T1) and bad environment (T2).

scholarly journals An evaluation of genetic distances for use with microsatellite loci.

Genetics ◽  
1995 ◽  
Vol 139 (1) ◽  
pp. 463-471 ◽  
Author(s):  
D B Goldstein ◽  
A Ruiz Linares ◽  
L L Cavalli-Sforza ◽  
M W Feldman
Keyword(s):  
Microsatellite Loci ◽  
Phylogenetic Reconstruction ◽  
Genetic Distances ◽  
Stepwise Mutation Model ◽  
Mutation Model ◽  
Stepwise Mutation ◽  
The Difference ◽  
Nei's Distance ◽  
Infinite Alleles Model ◽  
Overall Reliability

Abstract Mutations of alleles at microsatellite loci tend to result in alleles with repeat scores similar to those of the alleles from which they were derived. Therefore the difference in repeat score between alleles carries information about the amount of time that has passed since they shared a common ancestral allele. This information is ignored by genetic distances based on the infinite alleles model. Here we develop a genetic distance based on the stepwise mutation model that includes allelic repeat score. We adapt earlier treatments of the stepwise mutation model to show analytically that the expectation of this distance is a linear function of time. We then use computer simulations to evaluate the overall reliability of this distance and to compare it with allele sharing and Nei's distance. We find that no distance is uniformly superior for all purposes, but that for phylogenetic reconstruction of taxa that are sufficiently diverged, our new distance is preferable.

scholarly journals Transcriptomic analysis of the immune response against A. hydrophila infection in striped catfish Pangasianodon hypophthalmus

Aquaculture ◽  
2021 ◽  
pp. 737446
Author(s):  
Zhuo-hao Ruan ◽  
Liang-sen Jiang ◽  
Yi-fu Li ◽  
Zhi-qiang Lu ◽  
Xian-can Chen ◽  
...  
Keyword(s):  
Immune Response ◽  
Transcriptomic Analysis ◽  
Pangasianodon Hypophthalmus ◽  
Striped Catfish

Dietary ascorbic acid requirement for growth of striped catfish, Pangasianodon hypophthalmus (Sauvage, 1878) juveniles

2017 ◽  
Vol 24 (1) ◽  
pp. 616-624 ◽  
Author(s):  
N. Daniel ◽  
A.P. Muralidhar ◽  
P.P. Srivastava ◽  
K.K. Jain ◽  
K. Pani Prasad ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Pangasianodon Hypophthalmus ◽  
Striped Catfish

Seed production practices of striped catfish, Pangasianodon hypophthalmus in the Mekong Delta region, Vietnam

Aquaculture ◽  
2010 ◽  
Vol 306 (1-4) ◽  
pp. 92-100 ◽  
Author(s):  
Tam M. Bui ◽  
Lam T. Phan ◽  
Brett A. Ingram ◽  
Thuy T.T. Nguyen ◽  
Geoff J. Gooley ◽  
...  
Keyword(s):  
Seed Production ◽  
Mekong Delta ◽  
Delta Region ◽  
Pangasianodon Hypophthalmus ◽  
Striped Catfish ◽  
Production Practices

scholarly journals Monitoring of reproductive health in the striped catfish Pangasianodon hypophthalmus (Sauvage, 1878) from the Saen Saep Canal, Thailand

2021 ◽  
Vol 20 (1) ◽  
pp. 61-72
Author(s):  
Thawanrat Puttipong ◽  
◽  
Sinlapachai Senarat ◽  
Jes Kettratad ◽  
Chitchai Chantangsi ◽  
...  
Keyword(s):  
Reproductive Health ◽  
Industrial Effluents ◽  
Reproductive Status ◽  
Polluted Water ◽  
Canal System ◽  
Vacuolar Degeneration ◽  
Pangasianodon Hypophthalmus ◽  
Eosinophilic Cytoplasm ◽  
Canal Systems ◽  
Striped Catfish

Khlong Saen Saep is one of the most important and large canal systems in Bangkok, Thailand. However, the emergence of heavily polluted water has occasionally been reported due to the domestic sewage and industrial effluents discharged into the system. This situation may affect the reproductive status of aquatic lives residing in this canal. To evaluate the reproductive status of the canal inhabitant, the striped catfish Pangasianodon hypophthalmus (Sauvage, 1878)—a common dweller of the Saen Saep Canal—was collected from two selected stations, the Bumpen Nua Temple and the Kamalun Islam Mosque. The fish gonads were then anatomically and histologically examined. The results indicated that neither parasites nor ovary with external abnormality were found. Nevertheless, several histological alterations were detected, including vacuolar degeneration of previtellogenic oocytes, accumulation of melanomacrophage centers (MMCs), and atretic oocytes. Moreover, severe histopathological changes, such as the eosinophilic cytoplasm of spermatogonia, MMCs, blood congestion, and syncytium of spermatozoa, were observed in the fish testis. Our study suggested that the environmental stress and pollutions previously reported from the Saen Saep Canal possibly underpin the gonadal impairments of P. hypophthalmus examined and may have adverse impact on the reproductive health of others living in this canal system

scholarly journals Haemoglobin polymorphism in selected farm animals: A review

2014 ◽  
Vol 30 (3) ◽  
pp. 377-390 ◽  
Author(s):  
S.S.A. Egena ◽  
R.O. Alao
Keyword(s):  
Genetic Improvement ◽  
Raw Materials ◽  
Farm Animals ◽  
Starch Gel Electrophoresis ◽  
Gene Frequencies ◽  
Starch Gel ◽  
And Performance ◽  
Polypeptide Chains ◽  
Degree Of Similarity

Biochemical diversity or polymorphism is the occurrence of varieties attributed to biochemical differences which are under genetic control. It has created a leeway for the genetic improvement of farm animals. This is because it can be used as a useful tool for the characterization of livestock breeds and population. This way, the degree of similarity or differences within and between breeds can be ascertained and this differences or similarity are important raw materials for genetic improvement of animals. Data obtained on gene frequencies and genotypes through polymorphism study makes it not only possible to compare the gene stocks of animals, the possible effects of the genes on reproductive and performance traits, but also study genetic variability under different environmental conditions of selection. This study was carried out to review haemoglobin (Hb) polymorphism in selected farm animals with the view of finding out the type of polymorphism observed by starch gel electrophoresis due to variation in the amino acid sequence in the polypeptide chains of Hb. The review showed clearly that there is a gene-controlled diversity in the different farm animals considered. This could serve as a reference point for future studies earmarked for the improvement of the animals possibly via marker-assisted selection.

Sign in / Sign up

Export Citation Format

Share Document