DNA BARCODING OF FRESHWATER FISH SPECIES OF MANNA RIVER (BENGKULU) AND SEMANKA RIVER (LAMPUNG)

The identification of fish species is challenging. DNA barcoding provides new perspective in ecology and systematics of fishes. In this study, we assess intraspecific and interspecific genetic divergence among several freshwater fish species from Manna River of Bengkulu Province and Semanka River of Lampung Province, using data from mitochondrial cytochrome c oxidase subunit I (COI) gene. The results show that a total of six freshwater fish species identified in this study exhibited high levels of interspecific variation based on 51 analyzed sequences. Almost all species corresponded to a single species, cohesive array of barcode sequences distinct from other species. Based on Kimura Two Parameters (K2P) within species, mean pairwise sequence distances comparisons range from 0.3 to 6.3%, while interspecific comparisons were much higher range between 1.7 to 29.4%, except species Tor macrolepis and Tor putitora showed high levels of genetic similarities and no reciprocal monophyly. Using this method will obviously allow the identification eggs, larvae, fillets and fins of freshwater fish.

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DNA barcoding common non-native freshwater fish species in Turkey: Low genetic diversity but high population structuring

Mitochondrial DNA ◽

10.3109/19401736.2012.748041 ◽

2013 ◽

Vol 24 (3) ◽

pp. 276-287 ◽

Cited By ~ 10

Author(s):

Emre Keskin ◽

Sevan Ağdamar ◽

Ali˙ Serhan Tarkan

Keyword(s):

Genetic Diversity ◽

Freshwater Fish ◽

Dna Barcoding ◽

Fish Species ◽

High Population ◽

Freshwater Fish Species ◽

Low Genetic Diversity ◽

Population Structuring

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DNA barcoding of economically important freshwater fish species from north-central Nigeria uncovers cryptic diversity

Ecology and Evolution ◽

10.1002/ece3.4210 ◽

2018 ◽

Vol 8 (14) ◽

pp. 6932-6951 ◽

Cited By ~ 15

Author(s):

Oluyinka A. Iyiola ◽

Lotanna M. Nneji ◽

Moshood K. Mustapha ◽

Chioma G. Nzeh ◽

Segun O. Oladipo ◽

...

Keyword(s):

Freshwater Fish ◽

Dna Barcoding ◽

Fish Species ◽

Cryptic Diversity ◽

North Central ◽

Freshwater Fish Species

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A review of the freshwater fishes of the Kimberley region of Western Australia

Zootaxa ◽

10.11646/zootaxa.2816.1.1 ◽

2011 ◽

Vol 2816 (1) ◽

pp. 1 ◽

Cited By ~ 9

Author(s):

DAVID L. MORGAN ◽

GERALD R. ALLEN ◽

BRADLEY J. PUSEY ◽

DAMIEN W. BURROWS

Keyword(s):

Freshwater Fish ◽

River Basin ◽

Fish Species ◽

Western Australia ◽

Single Species ◽

Freshwater Fishes ◽

Gambusia Holbrooki ◽

Freshwater Fish Species ◽

Introduced Fishes ◽

Eastern Mosquitofish

This paper provides an overview of the freshwater fishes of the remote and sparsely populated Kimberley region of Western Australia, an area that has been subject to minimal scientific surveys, most of which have occurred in the last 30 years. A total of 49 freshwater fish species are reported from the region, but this number will likely grow as a result of future discoveries. It is an endemic hotspot, with ~40% of the species found nowhere else; many of which are known from only a few localities. The fauna is dominated by members of the Terapontidae (10 species) and Eleotridae (10 species), followed by the Plotosidae (five species), Melanotaeniidae (five species), Atherinidae (four species) and Ambassidae (four species). Additionally, in terms of freshwater fishes of the Kimberley, there are two species each in the Toxotidae and Ariidae, and a single species from each of the Anguillidae, Clupeidae, Hemiramphidae, Belonidae, Apogonidae, Gobiidae and Soleidae. There are currently no introduced fishes found in any major catchments of the Kimberley, however, there are records of the Eastern Mosquitofish (Gambusia holbrooki) from Cape Leveque (Morgan et al. 2004c) and the Redclaw Crayfish (Cherax quadquicarinatus), which have recently been found within the Ord River basin (Doupé et al. 2004).

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Genetic Identification of Freshwater Fish Species Through DNA Barcoding from Lake Lebo Taliwang, West Nusa Tenggara

Journal of Tropical Biodiversity and Biotechnology ◽

10.22146/jtbb.46256 ◽

2019 ◽

Vol 4 (3) ◽

pp. 107 ◽

Cited By ~ 2

Author(s):

Tuty Arisuryanti ◽

Rika Lathif Hasan ◽

Khadija Lung Ayu ◽

Nofita Ratman ◽

Lukman Hakim

Keyword(s):

Freshwater Fish ◽

Dna Barcoding ◽

Fish Species ◽

Dna Analysis ◽

Mitochondrial Gene ◽

Genetic Identification ◽

Anabas Testudineus ◽

Freshwater Fish Species ◽

Three Samples ◽

Fish Samples

Lake Lebo Taliwang is one of the lakes in the West Nusa Tenggara with high freshwater fish species diversity. However, the species identification of freshwater fish species from Lake Lebo Taliwang using DNA barcoding is very limited. Therefore, the objective of this study was to identify seven samples of freshwater fish species collected from Lake Lebo Taliwang based on COI mitochondrial gene as a DNA barcoding marker and establish library COI sequences of Indonesian freshwater fish. We are using a standard DNA analysis and data obtained from this study was then examined using Nucleotide BLAST and the phylogenetic tree was analyzed using the Neighbour-Joining (NJ) method with Kimura 2 Parameter (K2P) model. The results revealed that among the seven samples of freshwater fishes collected from the Lake Lebo Taliwang, three samples were identified as Anabas testudineus, the other three samples were Barbodes binotatus and one sample was Trichopodus trichopterus. The level of similarity of these freshwater fish samples referred to the database from the GenBank and BOLD was between 98-100%. The NJ tree supports the clade of each species identified in this study. This occurrence indicated that DNA barcoding by using the COI mitochondrial gene was proven to be able to identify the freshwater fish samples accurately.

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