Genetic Identification of Freshwater Fish Species Through DNA Barcoding from Lake Lebo Taliwang, West Nusa Tenggara

Lake Lebo Taliwang is one of the lakes in the West Nusa Tenggara with high freshwater fish species diversity. However, the species identification of freshwater fish species from Lake Lebo Taliwang using DNA barcoding is very limited. Therefore, the objective of this study was to identify seven samples of freshwater fish species collected from Lake Lebo Taliwang based on COI mitochondrial gene as a DNA barcoding marker and establish library COI sequences of Indonesian freshwater fish. We are using a standard DNA analysis and data obtained from this study was then examined using Nucleotide BLAST and the phylogenetic tree was analyzed using the Neighbour-Joining (NJ) method with Kimura 2 Parameter (K2P) model. The results revealed that among the seven samples of freshwater fishes collected from the Lake Lebo Taliwang, three samples were identified as Anabas testudineus, the other three samples were Barbodes binotatus and one sample was Trichopodus trichopterus. The level of similarity of these freshwater fish samples referred to the database from the GenBank and BOLD was between 98-100%. The NJ tree supports the clade of each species identified in this study. This occurrence indicated that DNA barcoding by using the COI mitochondrial gene was proven to be able to identify the freshwater fish samples accurately.

Download Full-text

DNA barcoding common non-native freshwater fish species in Turkey: Low genetic diversity but high population structuring

Mitochondrial DNA ◽

10.3109/19401736.2012.748041 ◽

2013 ◽

Vol 24 (3) ◽

pp. 276-287 ◽

Cited By ~ 10

Author(s):

Emre Keskin ◽

Sevan Ağdamar ◽

Ali˙ Serhan Tarkan

Keyword(s):

Genetic Diversity ◽

Freshwater Fish ◽

Dna Barcoding ◽

Fish Species ◽

High Population ◽

Freshwater Fish Species ◽

Low Genetic Diversity ◽

Population Structuring

Download Full-text

DNA barcoding of economically important freshwater fish species from north-central Nigeria uncovers cryptic diversity

Ecology and Evolution ◽

10.1002/ece3.4210 ◽

2018 ◽

Vol 8 (14) ◽

pp. 6932-6951 ◽

Cited By ~ 15

Author(s):

Oluyinka A. Iyiola ◽

Lotanna M. Nneji ◽

Moshood K. Mustapha ◽

Chioma G. Nzeh ◽

Segun O. Oladipo ◽

...

Keyword(s):

Freshwater Fish ◽

Dna Barcoding ◽

Fish Species ◽

Cryptic Diversity ◽

North Central ◽

Freshwater Fish Species

Download Full-text

Branchial Chamber and Gastrointestinal Tracts Parasites of Fish Species in Rivers Niger and Benue at Lokoja, North Central, Nigeria

10.21203/rs.3.rs-145833/v1 ◽

2021 ◽

Author(s):

Agatha Eleojo Onoja-Abutu ◽

Martin Abdubala Okpanachi ◽

Clement Ameh Yaro ◽

Omaima Nasif ◽

Sulaiman Ali Alharbi ◽

...

Keyword(s):

Freshwater Fish ◽

Fish Species ◽

Physiological Saline ◽

T Test ◽

Test Results ◽

Chi Square ◽

North Central ◽

Freshwater Fish Species ◽

Fish Samples

Abstract Background Freshwater fish species are posing health challenges by either serving as host of parasites that are harmful to man and animals. This study was conducted to determine the parasitic profile of the branchial and gastrointestinal tracts of freshwater fish species from Niger and Benue Rivers at Lokoja. Fish samples were collected within a period of 12 months, the species and sex of the fish were identified. The two opercula of each fish were removed and examined for parasites, parasites recovered were preserved in 0.7% physiological saline. Gastrointestinal tracts were removed from each fish, placed in physiological saline and examined microscopically for parasites. Prevalence of parasites was calculated and comparison among categories was done using chi-square and student t-test. Results A total of seven parasites species were harboured in branchial chamber and gastrointestinal tracts of fish species from Rivers Niger and Benue at Lokoja; Clinostomum sp. (trematode), Camallanus sp. (nematode), Capillaria sp., Rhabdochona congolensis (nematode), Contracaecum sp. (nematode), Proteocephalus sp. (cestode) and Spinitectus guntheri. T. zilli 81(95.3%) had the highest prevalence while O. niloticus, Clarias sp., S. schall and M. atlanticus had prevalence of 87(89.7%), 96(80.0%), 48(70.6%) and 27(38.6%) respectively with prevalence of 191(80.3%) and 148(73.3%) in females and males respectively. The gastrointestinal tracts 287(65.2%) was significantly harboured more parasites than branchial chamber 52(11.8%). Conclusion Fish species in Rivers Niger and Benue are highly infected with parasites. Fish consumers should subject the fish to proper processing before consumption, as some of these parasites maybe of zoonotic importance.

Download Full-text

DNA BARCODING OF FRESHWATER FISH SPECIES OF MANNA RIVER (BENGKULU) AND SEMANKA RIVER (LAMPUNG)

Indonesian Fisheries Research Journal ◽

10.15578/ifrj.19.1.2013.9-17 ◽

2013 ◽

Vol 19 (1) ◽

pp. 9 ◽

Cited By ~ 1

Author(s):

Arif Wibowo ◽

Achmad Farajalah ◽

Husnah Husnah

Keyword(s):

Freshwater Fish ◽

Dna Barcoding ◽

Fish Species ◽

Single Species ◽

Interspecific Variation ◽

Coi Gene ◽

Reciprocal Monophyly ◽

Freshwater Fish Species ◽

Interspecific Comparisons ◽

Two Parameters

The identification of fish species is challenging. DNA barcoding provides new perspective in ecology and systematics of fishes. In this study, we assess intraspecific and interspecific genetic divergence among several freshwater fish species from Manna River of Bengkulu Province and Semanka River of Lampung Province, using data from mitochondrial cytochrome c oxidase subunit I (COI) gene. The results show that a total of six freshwater fish species identified in this study exhibited high levels of interspecific variation based on 51 analyzed sequences. Almost all species corresponded to a single species, cohesive array of barcode sequences distinct from other species. Based on Kimura Two Parameters (K2P) within species, mean pairwise sequence distances comparisons range from 0.3 to 6.3%, while interspecific comparisons were much higher range between 1.7 to 29.4%, except species Tor macrolepis and Tor putitora showed high levels of genetic similarities and no reciprocal monophyly. Using this method will obviously allow the identification eggs, larvae, fillets and fins of freshwater fish.

Download Full-text

Branchial Chamber and Gastrointestinal Tracts Parasites of Fish Species in Benue and Niger Rivers, North Central, Nigeria

International Journal of Zoology ◽

10.1155/2021/6625332 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Agatha Eleojo Onoja-Abutu ◽

Martin Abdubala Okpanachi ◽

Luay Alkazmi ◽

Clement Ameh Yaro ◽

Gaber El-Saber Batiha

Keyword(s):

Freshwater Fish ◽

Fish Species ◽

Physiological Saline ◽

T Test ◽

Body Parts ◽

Chi Square ◽

Freshwater Fish Species ◽

Significant Difference ◽

Fish Samples ◽

Chi Square Test

Freshwater fish species are posing health challenges by either serving as host of parasites that are harmful to man and animals. This study was conducted to determine the parasitic profile of the branchial and gastrointestinal tracts of freshwater fish species from Niger and Benue rivers at Lokoja. These fish species are Tilapia zillii, Oreochromis niloticus, Clarias species, Synodontis schall, and Megalops atlanticus. Fish samples were collected within a period of 12 months, and the species and sex of the fish were identified. The two opercula of each fish were removed and examined for parasites; parasites recovered were preserved in 0.7% physiological saline. Gastrointestinal tracts were removed from each fish, placed in physiological saline, and examined microscopically for parasites. Prevalence of parasites was calculated. The chi-square test was used to compare prevalence of parasites according to sexes, body parts examined, fish species, and parasites species, while the t-test was used to compare between gills and intestines of each fish species. The chi-square test was used to determine if any significant difference exists in prevalence between the sexes, body parts, fish species, and parasites species, while the t-test was used to determine if any significant difference exists in prevalence between the gills and intestines of each fish species. A total of seven parasites species were harboured in branchial chamber and gastrointestinal tracts of fish species from rivers Niger and Benue: Clinostomum sp. (trematode), Camallanus sp. (nematode), Capillaria sp., Rhabdochona congolensis (nematode), Contracaecum sp. (nematode), Proteocephalus sp. (cestode), and Spinitectus guntheri. T. zillii (81 (95.3%)) had the highest prevalence, while O. niloticus, Clarias sp., S. schall, and M. atlanticus had prevalence of 87 (89.7%), 96 (80.0%), 48 (70.6%), and 27 (38.6%), respectively. Out of the total fish samples examined, females (191 (80.3%)) were observed to harbour more parasites than males (148 (73.3%)). The gastrointestinal tracts (287 (65.2%)) significantly harboured more parasites than branchial chamber (52 (11.8%)). Fish species in rivers Niger and Benue are highly infected with parasites. Fish consumers should subject the fish to proper processing before consumption, as some of these parasites are of zoonotic importance.

Download Full-text