scholarly journals Increasing Chitinase Activity of Serratia marcescens PT-6 through Optimization of Medium Composition

2019 ◽  
Vol 14 (3) ◽  
pp. 113
Author(s):  
Akhmad Awaludin Agustiar ◽  
Imas Faturrohmah ◽  
Bekti Wulan Sari ◽  
Nurul Binti Isnaini ◽  
Indun Dewi Puspita ◽  
...  
Keyword(s):  
Carbon Source ◽  
Serratia Marcescens ◽  
Nitrogen Source ◽  
Yeast Extract ◽  
Value Added ◽  
Chitinase Activity ◽  
Medium Composition ◽  
Colloidal Chitin ◽  
Additional Carbon Source ◽  
Additional Nitrogen

Chitin hydrolysate is one of the value added product derived from shrimp shell waste. Production of chitin hydrolysate using biological process offers an environmental friendly method compared to chemical process. Serratia marcescens PT-6, a gram negative chitinolytic bacterium isolated from shrimp pond sediment, shows good activity in hydrolyzing chitin. This study aimed to improve the chitinase activity of S. marcescens PT-6 culture by optimizing the component of chitin-containing medium (additional nitrogen source, additional carbon source, and colloidal chitin). The optimization of chitinase by S. marcescens PT-6 culture was done using one variable at a time method. The sequence of the research were to optimize 1) the type of additional carbon source (glucose, lactose, sucrose, and starch), 2) the type of additional nitrogen source (yeast extract, peptone, ammonium sulphate, and ammonium chloride), 3) the concentration of colloidal chitin (0.5; 1; 1.5; 2; and 2.5%), and 4) the concentration of the additional carbon and nitrogen source. The culture of S. marcescens PT-6 was incubated in colloidal chitin medium at 30 oC and chitinase activity from culture supernatant was analyzed. The results showed that starch gave the highest chitinase activity compare to other carbon source, meanwhile yeast extract was chosen as the best nitrogen source among others. The combination of 1.5% colloidal chitin with 0.5% starch and 0.1% yeast extract in medium increased the chitinase activity of S. marcescens PT-6 to 0.021 U/ml. These results indicated that an appropriate medium composition could increase the chitinase activity produced by S. marcescens PT-6 culture.

scholarly journals Optimization of Agitation Rate in Bioreactor Increases Chitinase Activity of Serratia marcescens PT6

2020 ◽  
Vol 147 ◽  
pp. 03019
Author(s):  
Amara Faiz Wriahusna ◽  
Niswah Umhudloh Dzakiyya ◽  
Indun Dewi Puspita ◽  
Sri Pudjiraharti
Keyword(s):  
Serratia Marcescens ◽  
Bacterial Growth ◽  
Agar Medium ◽  
Chitinase Activity ◽  
Colloidal Chitin ◽  
Gram Negative Bacteria ◽  
Agitation Rate ◽  
Anova Analysis ◽  
Gram Negative ◽  
Shrimp Pond

Serratia marcescens PT6 is a Gram-negative bacteria isolated from shrimp pond sediment that capable of producing chitinase. This study aimed to observe the effect of agitation rate on growth and chitinase activity of S. marcescens PT-6 in a bioreactor. The production of chitinase was done in 1.5 l bioreactor using colloidal chitin broth at the condition of pH 7, the temperature of 30°C, aeration of 0.04 vvm, and variation of agitation rate (200, 350, 500 rpm). Bacterial growth was measured by colonies counting in agar medium, while chitinase activity was measured by means of colorimetric every day for four days incubation. The results of ANOVA analysis show that the agitation rate had no effect on bacterial growth, but a significant effect (P<0.05) was observed on chitinase activity. The highest growth and chitinase activity were obtained at 200 rpm, with the highest chitinase activity of 0.006 ± 0.001 U/ml was at day-2. This study implies that the optimized agitation rate in the bioreactor increased the chitinase activity produced by S. marcescens PT-6.

Optimization of Xylanase Production by Streptomyces costaricanus 45I-3 Using Various Substrates through Submerged Fermentation

2020 ◽  
Vol 14 (1) ◽  
pp. 5
Author(s):  
SIPRIYADI SIPRIYADI ◽  
ARIS TRI WAHYUDI ◽  
MAGGY THENAWIDJAYA SUHARTONO ◽  
ANJA MERYANDINI
Keyword(s):  
Nitrogen Source ◽  
Yeast Extract ◽  
Xylanase Activity ◽  
Hydrolytic Enzymes ◽  
Tween 80 ◽  
Medium Composition ◽  
Production Costs ◽  
Xylanase Production ◽  
Beechwood Xylan

Xylanase is an important hydrolytic enzymes with many application in several industries, but to obtain enzyme derived products is not easy. Thus, the optimization of efficient xylanases production is a great interest for biotechnological application. This study aims to determine the type of substrate, medium composition, and optimum conditions of xylanase production by S. costaricanus 45I-3. Determination of substrate type was done by growing the tested bacteria on birchwood xylan, beechwood xylan, oat spelled xylan, corn cobs xylan, and tobacco xylan substrate, meanwhile the determination of medium composition and enzyme production were done by measuring xylanase activity at various substrate concentration and replacing the carbon, nitrogen, phosphate and surfactants source. The results showed that the highest enzymatic index (EI) produced from corn cob xylan substrate at 3.60 meanwhile the second highest was beechwood xylan substrate at 2.87 EI, however this substrate is purer, thus this substrate was selected and used as xylan sources for further optimization measurement. The best xylanase activity (2.29 U/mL) obtained on eighth day after inoculation on rotary incubator at 120 rpm in 28 ºC. Arabinose as the source of carbon generate the highest activity at 3.161 U/mL meanwhile the most preferred source of phosphate is Na2HPO4 (2.37 U/mL). Both source of nitrogen i.e. nitrogen ammonium sulphate (NH4)2SO4 and yeast extract were able to produce xylanase at 2.57 and 2.36 U/mL. The addition of surfactant in production medium showed addition of SDS surfactant (0.146 U/mL) and Tween 80 (0.438 U/mL) showed a negative response by decreasing the activity. The conclusion showed that the xylanase activity was increased after optimization at various C, N, and P sources, and the use of nitrogen source (NH4)2SO4), become a more economical alternative to replacing a nitrogen source yeast extract so it can lower the production costs of xylanase enzyme.

scholarly journals Mass Spectrometry Reveals Molecular Structure of Polyhydroxyalkanoates Attained by Bioconversion of Oxidized Polypropylene Waste Fragments

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1580 ◽  
Author(s):  
Brian Johnston ◽  
Iza Radecka ◽  
Emo Chiellini ◽  
David Barsi ◽  
Vassilka Ivanova Ilieva ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Mass Spectrometry ◽  
Carbon Source ◽  
Shake Flask ◽  
Value Added ◽  
Repeat Units ◽  
A Value ◽  
Cupriavidus Necator H16 ◽  
Oxidized Polypropylene ◽  
Additional Carbon Source

This study investigated the molecular structure of the polyhydroxyalkanoate (PHA) produced via a microbiological shake flask experiment utilizing oxidized polypropylene (PP) waste as an additional carbon source. The bacterial strain Cupriavidus necator H16 was selected as it is non-pathogenic, genetically stable, robust, and one of the best known producers of PHA. Making use of PHA oligomers, formed by controlled moderate-temperature degradation induced by carboxylate moieties, by examination of both the parent and fragmentation ions, the ESI-MS/MS analysis revealed the 3-hydroxybutyrate and randomly distributed 3-hydroxyvalerate as well as 3-hydroxyhexanoate repeat units. Thus, the bioconversion of PP solid waste to a value-added product such as PHA tert-polymer was demonstrated.

scholarly journals Effect of the medium composition on formation of amylase by Bacillus sp

2003 ◽  
Vol 46 (1) ◽  
pp. 129-134 ◽  
Author(s):  
Eliana de Oliveira. Santos ◽  
Meire Lelis Leal Martins
Keyword(s):  
Carbon Source ◽  
Yeast Extract ◽  
Soluble Starch ◽  
Medium Composition ◽  
Alpha Amylase ◽  
Bacillus Sp ◽  
Glucose Effect ◽  
Moderately Thermophilic ◽  
Exogenous Glucose ◽  
Culture Supernatants

Studies on the alpha -amylase synthesis was carried out with a moderately thermophilic, facultatively anaerobic Bacillus sp, isolated from soil samples. The cells were cultivated in a complex medium containing soluble starch or maltose as carbon source. The levels of the alpha -amylaseactivity detected in culture supernatants varied greatly with the type of carbon source used. Maltose, soluble starch and citrate stimulated alpha -amylaseformation. Addition of exogenous glucose repressed formation of alpha -amylase, demonstrating that a classical glucose effect was operative in this organism. The concentration of yeast extract was found to be important factor in the alpha -amylase synthesis bythe isolate.The activity of the enzyme increased between 2 and 5 g/L yeast extract concentration and then fell very rapidly beyond this point. The best concentration of peptone to alpha-amylase formation was found to be around 10g/L.

scholarly journals Optimization of conditions for extracellular amylase production from Listeria denitrificans

2013 ◽  
pp. 73-81
Author(s):  
MN Mahmud ◽  
MN Anwar
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Incubation Period ◽  
Yeast Extract ◽  
Medium Ph ◽  
Amylase Production ◽  
Extracellular Amylase

The foodborne Listeria denitrificans was isolated from degraded rice and studied as an amylase producer. This microorganism showed maximum amylase production at temperature 27 ºC and medium pH 7.0, and on 3rd day during incubation period. Maximum amylase production by the isolate was found in medium containing 3.0 % starch as carbon source and 0.50 % yeast extract as nitrogen source. DOI: http://dx.doi.org/10.3329/cujbs.v4i1.13388 The Chittagong Univ. J. B. Sci.,Vol. 4(1&2):73-81, 2009

scholarly journals Metabolome profiles of the alphaproteobacterial methanotroph Methylocystis sp. Rockwell in response to carbon and nitrogen source

2020 ◽  
Author(s):  
Marina Lazic ◽  
Scott Sugden ◽  
Dominic Sauvageau ◽  
Lisa Y Stein
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Metabolic Regulation ◽  
Large Scale ◽  
Metabolic Response ◽  
Low Cost ◽  
Critical Role ◽  
Value Added ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen

Abstract Methanotrophs use methane as a sole carbon source and thus play a critical role in its global consumption. Intensified interest in methanotrophs for their low-cost production of value-added products and large-scale industrialization has led to investigations of strain-to-strain variation in parameters for growth optimization and metabolic regulation. In this study, Methylocystis sp. Rockwell was grown with methane or methanol as a carbon source and ammonium or nitrate as a nitrogen source. The intracellular metabolomes and production of polyhydroxybutyrate, a bioplastic precursor, were compared among treatments to determine how the different combinations of carbon and nitrogen sources affected metabolite production. The methane-ammonium condition resulted in the highest growth, followed by the methane-nitrate, methanol-nitrate, and methanol-ammonium conditions. Overall, the methane-ammonium and methane-nitrate conditions directed metabolism toward energy-conserving pathways, while methanol-ammonium and methanol-nitrate directed the metabolic response toward starvation pathways. Polyhydroxybutyrate was produced at greater abundances in methanol-grown cells, independent of the nitrogen source. Together, the results revealed how Methylocystis sp. Rockwell altered its metabolism with different combinations of carbon and nitrogen source, with implications for production of industrially relevant metabolites.

Regulation of extracellular protease formation by Serratia marcescens

1979 ◽  
Vol 25 (1) ◽  
pp. 47-52 ◽  
Author(s):  
Bruno J. Bromke ◽  
Jay M. Hammel
Keyword(s):  
Carbon Source ◽  
Serratia Marcescens ◽  
Proteolytic Activity ◽  
Specific Activity ◽  
Partial Purification ◽  
Dibutyryl Cyclic Amp ◽  
Extracellular Proteases ◽  
Proteolytic System ◽  
Growing Cell ◽  
Additional Carbon Source

Heavy cell suspensions of Serratia marcescens, when grown in gelatin-containing media, produce extracellular proteases which increase in specific activity in a linear fashion for 3 to 4 h. During partial purification, a single peak of proteolytic activity was demonstrated by Sephadex G-100 chromatography. However, electrophoresis using 5% polyacrylamide gels discloses three proteolytically active bands. Evidence in favor of gelatin acting as an inducer of the 'proteolytic system' was provided by two observations. First, proteolytic activity is only present in media containing gelatin. Secondly, the addition of 10−4 M rifampicin to cells growing in gelatin-containing medium plus an additional carbon source inhibits protease activity totally, but has no effect on growth. When glycerol is added to a growing cell suspension in gelatin-containing medium, growth increases, but protease specific activity decreases. This 'glycerol effect' is not due to an accumulation of active or inactive enzyme in association with the cell, nor to a decrease in the total number of proteases synthesized. Rather, glycerol, as other utilizable carbohydrates, exerts a repression which can be eliminated by 5 mM dibutyryl cyclic AMP.

Culture Media for Enhanced Chitinase Production from Serratia marcescens Strain JPP1 and Stability of Crude Enzyme

2013 ◽  
Vol 726-731 ◽  
pp. 62-65
Author(s):  
Kai Wang ◽  
Pei Sheng Yan ◽  
Li Xin Cao
Keyword(s):  
Serratia Marcescens ◽  
Nitrogen Source ◽  
Culture Media ◽  
Inorganic Nitrogen ◽  
Peak Level ◽  
Tween 80 ◽  
Chitinase Activity ◽  
Antagonistic Activity ◽  
Inorganic Nitrogen Source ◽  
Chitinase Production

Serratia marcescens strain JPP1 was isolated from the peanut hulls in Jiangsu Province, China. The strain exhibited antagonistic activity against aflatoxins production and chitinolytic activity by producing chitinases. For chitinase production glucose was identified as best carbon source, peptone as organic nitrogen source while ammonium sulphate as the best inorganic nitrogen source led to the highest chitinase activity. Chitin served as an essential inducer and chitinase production reached the peak level after 1.2% of chitin concentration. The crude chitinase was highly thermostable and had activity in broad pH (2-11) and temperature (30-90°C) range. Ca and Mn ions enhanced the chitinase production, while Na and K were of inhibitory action not more than 5%, and Cu, Zn and Fe ions resulted in drastic enzyme inhibition ranged between 63.7 to 70.5%. Addition of EDTA, mercaptoethanol and Tween 80 had positive effect on chitinase production while SDS decreased the chitinase production by S. marcescens JPP1.

Studies on the Biological Characteristics of Termitomyces Albuminosus Hypha

2013 ◽  
Vol 709 ◽  
pp. 810-813 ◽  
Author(s):  
Xiong Ya ◽  
Min Jie Li
Keyword(s):  
Growth Factors ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Biological Characteristics ◽  
Edible Fungi ◽  
Growth Environment ◽  
Wild Edible Fungi ◽  
Development And Utilization

Termitomyces albuminosus is a kind of local distinctive wild edible fungi in southwest of China. It is delicious, rich in nutrition and has high development and utilization value, but owing to the restrictions of growth environment, it can not be cultivated artificially. This article mainly studied on the biological characteristics of Termitomyces albuminosus Hypha, and found out the optimal carbon source, nitrogen source, growth factors and the C/N ratio that are suitable for the growth of Hypha of Termitomyces albuminosus .

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