Influence of dry extract of brassica olearacea on the morphological structure of the gastric mucosa of rats during an experimental ulcer caused by alcohol-prednisolone mixture

The article presents the results of study of the search influence of the preparation «Shchytnyk» in the form of dry extract got from the roots of masculine ferns (Rhizoma Dryopteris filix–mas, order of many–stems –Polypodiaceae), on the coefficients of the mass of internal organs, some biochemical parameters of blood and morphological structure of rat liver in conditions of cumulation.To determine the cumulative properties of the preparation «Shchytnyk » was used test method «subchronical toxicity» . The experiment was conducted on 18 white rats, 2 – months old, with body mass of 140 – 160g, which were formed into two groups of animals– analogues: I – control, II – research (9 animals in each). To enter a drug it was dissolved in propylene glycol with water in a ratio of 1: 1. Rats of experimental drug were administered orally every day, starting with a dose of 1300 mg/kg (representing 1/10 DL50), followed by increasing doses of 1.5 times every 4 days. The animals from the control group were injected with solvent.The introduction of a new antiparasitis drug to rats «Shchytnyk» for 24 consecutive days at a total dose of 36196,9 mg/kg caused an increase coefficients in liver weight ratios, increased activity of alkaline phosphatase, liver transaminases in serum compared to control, that histologically confirmed dystrophic–necrotic changes in the parenchyma of the liver, and were characterized with the expansion of central venous and intra–lobular capillaries, cariorecsise, grit cytoplasm of hepatocytes.

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The effect of dry extract of cabbage garden on the local non-specific proteinase-inhibitory potential of the mucous membrane of the stomach on the model of acute gastric ulcer

Farmatsevtychnyi zhurnal ◽

10.32352/10.32352/0367-3057.6.19.09 ◽

2019 ◽

pp. 77-84

Author(s):

N. N. Kononenko ◽

M. T. Mirzaliev ◽

V. V. Chikitkina

Keyword(s):

Peptic Ulcer ◽

Gastric Ulcer ◽

Gastric Mucosa ◽

Proteinase Inhibitor ◽

Proteinase Inhibitors ◽

Dry Extract ◽

Inhibitor System ◽

Inhibitory Potential ◽

Acid Stable ◽

Cabbage Extract

Peptic ulcer of the stomach and duodenum is a complex gastroenterological disease multifactorial in pathogenesis. One of the key components of non-specific inflammation in peptic ulcer disease is the activation of proteinases in the gastric mucosa and their interaction with proteinase inhibitors, which determines the degree of proteolytic aggression in terms of the development of destructive changes in the tissues that they damage. The aim of the work was to study the effect of dry extract of cabbage garden on the components of the proteinase-inhibitor system in experimental gastric ulcer. The effect of dry extract of cabbage garden on the local non-specific proteinase-inhibitory potential of the gastric mucosa was studied on the model of acute alcohol-prednisolone ulcers. Dry extract of cabbage garden at a dose of 50 mg/kg and comparison drugs – omeprazole and altan were administered intragastrically once a day for 7 days from the second day of the experiment. On the 8th day, elastase-like, trypsin-like activity and acid-stable inhibitors in the swabs from the stomach and intestines, biopsy samples of the gastric mucosa from the esophagus and fundus, and small intestine were determined. It has been established that with an experimental alcohol-prednisolone gastric ulcer, a local non-specific proteinase-inhibitory system is involved in the mechanism of mucosal damage. At the same time, the level of trypsin-like and elastase-like activity of gastric juice increases and the activity of acid-stable inhibitors decreases, which normally suppress the enzymatic activity of proteinases. According to the effect on the non-specific proteinase-inhibitor system, the dry cabbage extract was significantly superior to altan and not inferior to omeprazole. The most pronounced effect of normalizing the activity of nonspecific proteinases and their inhibitors was established using a combination of dry cabbage extract with omeprazole. One of the mechanisms of the antiulcer action of the dry cabbage extract is its effect on the local nonspecific proteinase-inhibitory potential of the gastric mucosa. In the model of acute alcohol-prednisolone gastric ulcer, dry extract of cabbage garden caused a decrease in the activity of local non-specific proteolytic enzymes and activation of proteinase inhibitors of the gastric mucosa and duodenum, exceeding the activity of altan. Under the combined use of the dry extract of cabbage and omeprazole, a complete normalization of the state of the local non-specific proteinase-inhibitory potential of the gastroduodenal zone was observed.

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High-resolution topographic SEM and radiation damage: The rationale for using low beam voltage

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100131024 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1278-1279

Author(s):

James Pawley ◽

David Joy

Keyword(s):

High Resolution ◽

Imaging Techniques ◽

Morphological Structure ◽

Radiation Sensitivity ◽

Beam Specimen ◽

Measured Signal ◽

Practical Consideration ◽

Interaction Volume ◽

Impact Area ◽

Signal Contrast

The scanning electron microscope (SEM) builds up an image by sampling contiguous sub-volumes near the surface of the specimen. A fine electron beam selectively excites each sub-volume and then the intensity of some resulting signal is measured and then plotted as a corresponding intensity in an image. The spatial resolution of such an image is limited by at least three factors. Two of these determine the size of the interaction volume: the size of the electron probe and the extent to which detectable signal is excited from locations remote from the beam impact area. A third limitation emerges from the fact that the probing beam is composed of a number of discrete particles and therefore that the accuracy with which any detectable signal can be measured is limited by Poisson statistics applied to this number (or to the number of events actually detected if this is smaller). As in all imaging techniques, the limiting signal contrast required to recognize a morphological structure is constrained by this statistical consideration. The only way to overcome this limit is to increase either the contrast of the measured signal or the number of beam/specimen interactions detected. Unfortunately, these interactions deposit ionizing radiation that may damage the very structure under investigation. As a result, any practical consideration of the high resolution performance of the SEM must consider not only the size of the interaction volume but also the contrast available from the signal producing the image and the radiation sensitivity of the specimen.

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Ultrastructure Research of the Gastritis from Returning Bile

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100158558 ◽

1990 ◽

Vol 48 (3) ◽

pp. 202-203

Author(s):

Shaopeng Hu ◽

Jianhua Wang ◽

Zhen Li ◽

Huei Chen ◽

Fei Cu ◽

...

Keyword(s):

Electron Microscope ◽

Gastric Mucosa ◽

Scanning Electron Microscope ◽

Lamina Propria ◽

Transmission Electron Microscope ◽

Ultrastructural Change ◽

Common Disease ◽

Epithelial Surface ◽

Transmission Electron ◽

Scanning Electron

Gastritis from returning bile is a common disease, but the reason for the disease is not clear. As the pathologic ultrastructure research progresses, it has drawn attention to the ultrastructural change of cells in gastric mucosa by clinical workers. We observed gastric mucosa tissues of 15 patients suffering from gastritis with a transmission electron microscope (TEM) and a scanning electron microscope (SEM). It is the first report in China that fungus exists in the lamina propria of gastric mucosa tissue. The result is as follows.The gastric mucosa tissues of 15 patients suffering from gastritis were acquired by stomachoscopy. Both TEM and SEM specimens were prepared by the usual methods. Under the TEM, the epithelial surface became higher and larger. Mitochondria of the cells were swollen and cristae were disrupted. There were vacuoles in the cells. The nucleus showed disorder, heterochromatin became darker, and nucleolae could be observed.

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Sem Observations in Gastric Mucosa Exposed to Progressive Enzymatic Etching

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600002531 ◽

1980 ◽

Vol 38 ◽

pp. 570-571

Author(s):

C.A.E. Lemmi ◽

D. Booth ◽

G.E. Adomian

Keyword(s):

Gastric Mucosa ◽

Critical Point ◽

Proteolytic Activity ◽

Etching Process ◽

Cellular Types

In order to enrich populations of homogeneous cellular types we dissociated gastric mucosa by enzymatic techniques. In addition, we used SEM to monitor the progressive etching of the mucosa. Two enzymes were tested: collagenase III with minimum proteolytic activity and Pronase with broader proteolytic effects. The gastric mucosa was exposed to the effect of the enzymes using everted stomach preparations. In this way the digestive action occured progressively from the lumen of the stomach toward the base of the glands. This “etching” process could be monitored conveniently by SEM. After incubation for periods varying from 30 to 210 minutes the tissues were stretched on dental wax, fixed in 2 % glutaralheyde, post-fixed in osmium, dehydrated, critical point dryed and coated with gold. A model MSM-5 “Mini-SEM” was used for observation. Gentle uncurling of the preparation before coating with gold produced fractures which revealed the structure of the gastric glandsin more detail.

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