scholarly journals Biochemical markers of connective tissue metabolism in the diagnostics of respiratory diseases in human and animals: retrospective analysis (1984–2010)

2021 ◽  
pp. 30-35
Author(s):  
Dmytro Morozenko ◽  
Roman Dotsenko ◽  
Yevheniia Vashchyk ◽  
Andriy Zakhariev ◽  
Andrii Zemlianskyi ◽  
...  
Keyword(s):  
Veterinary Medicine ◽  
Connective Tissue ◽  
Bronchoalveolar Lavage ◽  
Respiratory System ◽  
Biochemical Markers ◽  
Respiratory Diseases ◽  
Diagnostic Information ◽  
Source Analysis ◽  
Tissue Metabolism ◽  
Connective Tissue Metabolism

The aim: to analyze the literature data for the period from 1984 to 2010 on the use of biochemical markers of disorders of connective tissue metabolism in diseases of the respiratory system in humans and animals. Materials and methods. The research was conducted by the method of scientific literature open source analysis: PubMed, Elsevier, electronic resources of the National Library named after V.I. Vernadsky (1984–2010). Results. In the case of diseases of the respiratory system in humans, the pathogenesis of pneumonia is the development of inflammation in the interstitial, peribronchial, perivascular and perilobular connective tissue, lymphatic vessels of the lungs, followed by involvement of alveoli and bronchioles in the inflammation. The morphological basis of these changes may be pneumofibrosis and pneumosclerotic changes. In the chronic course of pneumonia, chronic obstructive pulmonary disease develops. This pathology is closely related to the action of inflammatory cytokines that regulate connective tissue proliferation. Similar studies were performed on eosinophilic bronchopneumonia in dogs, but the material for the study was bronchoalveolar lavage. The current method of diagnosing respiratory diseases using cytokines (interleukin-4, interferon-γ) and bronchoalveolar lavage has no diagnostic information in chronic bronchitis and bronchial asthma in cats. Fundamental studies of connective tissue biopolymers in clinically healthy and bronchopneumonia piglets have recently been conducted in veterinary medicine. Conclusions. Recently, in medicine of particular interest to researchers is the determination of the content in biological fluids of indicators of connective tissue metabolism (hydroxyproline, glycosaminoglycans, glycoproteins, sialic acids) to diagnose diseases of the respiratory system. To diagnose connective tissue disorders in lung diseases in medical practice use indicators of oxyproline in serum and urine. Oxyproline is one of the most important components of lung collagen. An increase in the content of free oxyproline in the blood indicates an increased rate of collagen breakdown in the lung tissue. Analysis of oxyproline fractions, as indicators of the direction of collagen metabolism, allows to assess the condition of the connective tissue of the lungs and can serve as a prognostic criterion for the course of the disease. Thus, the indicators of connective tissue metabolism showed significant diagnostic information, which allowed to recommend them for use in the practice of veterinary medicine.

scholarly journals BIOCHEMICAL MARKERS OF CONNECTIVE TISSUE METABOLISM IN RATS’ BLOOD SERUM WITH EXPERIMENTAL KNEE OSTEOARTHRITIS AND THEIR DYNAMICS DURING CONSERVATIVE TREATMENT

2019 ◽  
Vol 72 (2) ◽  
pp. 193-197
Author(s):  
Kyrylo V. Makolinets ◽  
Vasyl I. Makolinets ◽  
Dmytro V. Morozenko ◽  
Kateryna V. Gliebova ◽  
Svitlana I. Danylchenko
Keyword(s):  
Laser Radiation ◽  
Conservative Treatment ◽  
Connective Tissue ◽  
Biochemical Markers ◽  
Diclofenac Sodium ◽  
Infrared Laser ◽  
Knee Joints ◽  
Glucosamine Hydrochloride ◽  
Tissue Metabolism ◽  
Connective Tissue Metabolism

Introduction: There are many methods for diagnosis and treatment of knee joints osteoarthritis in modern orthopedics. The infrared radiation is one of the most popular and effective methods though it needs experimental substantiation on rats. The aim: Experimental definition of the effectiveness of the proposed method of conservative treatment of knee joints osteoarthritis using sodium diclofenac, glucosamine hydrochloride and infrared laser radiation, on the basis of biochemical markers of connective tissue metabolism in rats. Materials and methods: Experimental studies were performed on 40 white male rats. A part of rats received infrared laser radiation in addition to diclofenac sodium and glucosamine hydrochloride. Results: Using a complex of infrared laser radiation and glucosamine hydrochloride and diclofenac sodium in conservative treatment of rats with experimentally induced knee joints osteoarthritis rats after 24 days of treatment. Treatment with the above-mentioned scheme resulted in a significant reduction in the content of glycoproteins (28.5%), chondroitin sulfates (26.1%), alkaline phosphatase activity (32.3%), and I and II fractions of glycosaminoglycans, namely chondroitin-6- (28.7%) and chondroitin-4-sulfate (40.3%). Moreover, these indicators of the studied markers approached the level of intact animals. Conclusions: Changes in biochemical markers of connective tissue in rats during experimental knee joints osteoarthritis indicated a higher efficiency of the treatment regimen with the use of low-intensity infrared laser radiation compared with exclusively drug therapy.

scholarly journals DYNAMICS OF BIOCHEMICAL MARKERS OF CONNECTIVE TISSUE METABOLISM IN PATIENTS WITH KNEE OSTEOARTHRITIS DURING CONSERVATIVE TREATMENT WITH LASER THERAPY

2019 ◽  
Vol 72 (5) ◽  
pp. 802-806
Author(s):  
Kyrylo V. Makolinets ◽  
Vasyl I. Makolinets ◽  
Dmytro V. Morozenko ◽  
Kateryna V. Gliebova ◽  
Svitlana I. Danylchenko
Keyword(s):  
Conservative Treatment ◽  
Connective Tissue ◽  
Knee Osteoarthritis ◽  
Blood Serum ◽  
Laser Therapy ◽  
Biochemical Markers ◽  
Diclofenac Sodium ◽  
Glucosamine Hydrochloride ◽  
Tissue Metabolism ◽  
Connective Tissue Metabolism

Introduction: The biochemical markers of the connective tissue metabolism in blood serum may characterize the disturbances in inflammatory, destructive processes, and also serve the criteria for diagnostic evaluation of the state of cartilage and bone tissue of the joints in knee osteoarthritis to control the effectiveness of conservative treatment. The aim: determination of biochemical markers of connective tissue metabolism in the blood of patients with the 1st and 2nd stages of knee osteoarthritis in order to prove the effectiveness of the complex conservative treatment of patients with the use of low-intensity infrared laser radiation. Materials and methods: During the study we examined 80 patients with the 1st and 2nd stages of knee osteoarthritis according to the Kellgren and Lawrence classification. Some patients were prescribed diclofenac sodium and glucosamine hydrochloride, others were prescribed an additional course of laser therapy. Results: At the end of the treatment the group of patients with knee osteoarthritis who were treated with the use of laser therapy, diclofenac sodium and glucosamine hydrochloride, showed a decrease in the content of the following blood serum components: glycoproteins (by 28.9 %), sialic acids (by 28.8 %), chondroitinsulfates (by 91.3 %) due to chondroitin-6-sulfate, activity of alkaline phosphatase (by 36.1 %) compared with the indicators before treatment. Conclusions: a more significant decrease in the inflammation and destruction of the cartilage tissue of the affected joints was observed during knee osteoarthritis treatment with the use of laser therapy than without it.

Expression of mRNAs related to connective tissue metabolism in rat hepatic stellate cells and myofibroblasts

2007 ◽  
Vol 58 (4) ◽  
pp. 263-273 ◽  
Author(s):  
Alena Jiroutova ◽  
Rastislav Slavkovsky ◽  
Martina Cermakova ◽  
Lenka Majdiakova ◽  
Irena Hanovcova ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Tissue Metabolism ◽  
Connective Tissue Metabolism

scholarly journals Role of lysyl oxidase like 1 in regulation of postpartum connective tissue metabolism in the mouse vagina†

2019 ◽  
Vol 101 (5) ◽  
pp. 916-927 ◽  
Author(s):  
Ali Borazjani ◽  
Bruna M Couri ◽  
Mei Kuang ◽  
Brian M Balog ◽  
Margot S Damaser
Keyword(s):  
Gene Expression ◽  
Enzyme Activity ◽  
Connective Tissue ◽  
Lysyl Oxidase ◽  
Gelatin Zymography ◽  
Pelvic Organ ◽  
Tissue Metabolism ◽  
Reverse Transcription Pcr ◽  
Connective Tissue Metabolism

Abstract Pelvic organ prolapse (POP) in lysyl oxidase like-1 knockout (Loxl1 KO) mice occurs primarily in parous mice and is rare in nulliparous mice. We determined the effect of Loxl1 deficiency on postpartum regulation of connective tissue metabolism genes and degradative enzyme activity in the vagina at 20 days gestation or 4 h, 48 h, 7 days, 15 days, 25 days, 7 weeks, or 12 weeks postpartum. Nulliparous Loxl1 KO and wildtype (WT) mice aged 11, 18, or 23 weeks were controls. Gene expression and enzyme activity were assessed using real-time quantitative reverse transcription PCR and fluorescein conjugated gelatin zymography, respectively. Parity, but not aging, had a significant influence on gene expression both with time postpartum and between KO and WT mice. Mmp2, Timp1, Timp2, Timp3, Timp4, Col1a1, Col3a1, Acta2, and Bmp1 were differentially expressed between KO and WT mice. Correlational analysis of gene-gene pairs revealed 10 significant differences between parous KO and WT groups, 5 of which were due to lack of co-expression of Bmp1 in KO mice. The overall enzyme activity that could be attributed to MMPs was significantly higher in WT compared to KO mice both 25 days and 12 weeks postpartum, and MMP activity was significantly lower 15 days and 25 days postpartum compared to KO nulliparous controls, but not WT. These findings suggest that Loxl1 deficiency combined with parity has a significant impact on postpartum regulation of connective tissue metabolism, particularly as it relates to co-expression of Bmp1 and altered proteolytic activity.

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