scholarly journals The effect of biotic and abiotic elicitors on biomass and saponin production of secondary root cultivated in shake flasks Panax vietnamensis adventitious root

2018 ◽  
Vol 15 (2) ◽  
pp. 285-291
Author(s):  
Nguyễn Thị Nhật Linh ◽  
Hoàng Thanh Tùng ◽  
Nguyễn Hoàng Lộc ◽  
Dương Tấn Nhựt
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Yeast Extract ◽  
Adventitious Root ◽  
Secondary Root ◽  
Abiotic Elicitor ◽  
Shake Flasks ◽  
Modified Ms Medium ◽  
Resistance To Stress ◽  
Abiotic Elicitors

Panax vietnamensis Ha et Grushv. is an endemic ginseng of Vietnam which has highly nutraceutical, medicinal and comercial values. Many its special saponins provide resistance to stress, disease and exhaustion. In this study, the biotic elicitors (yeast extract and chitosan) at concentrations from 50 – 200 mg/l and abiotic elicitors (jasmonic acid, abscisic acid and salicylic acid) at concentrations from 50 – 200 µl/l were used to evaluate the possibility of increasing secondary root’s biomass and saponin in Panax vietnamensis adventitious root cultures. This cultures were maintained on Innova 2100 shaker shaker plantform at a speed of 100 rpm, and its modified MS medium (NH4+/NO3-: 7.19/18.5 mM/mM) were supplemented with 7 mg/l IBA, 0.5 mg/l BA and 3% sucrose. Results after 56 days of cultured showed that most elicitors significantly reduced dry biomass of secondary roots as compared to control (exception the addition of 50 – 100 mg/l yeast extract or 50 µl/l salicylic acid). However, all elicitors increased saponin-accumulation, and abiotic elicitor is more effective saponin-accumulation than those biotic elicitors. Jasmonic acid obviously gave the best results; with total amount of 3 saponins at 150 µl/l, Rg1 at 100 µl/l, Rb1 at 50 µl/l and MR2 at 200 µl/l and Rg1 (3.22%), significantly higher than other concentration of elicitor. However, for accumulating saponins and developing secondary root, 150 mg/l YE is the most real effective in all elicitors (0.88 mg).

Effect of salicylic acid and yeast extract on the accumulation of jasmonic acid and sesquiterpenoids in Panax ginseng adventitious roots

2014 ◽  
Vol 61 (6) ◽  
pp. 811-817 ◽  
Author(s):  
S. Rahimi ◽  
B. S. R. Devi ◽  
A. Khorolragchaa ◽  
Y. J. Kim ◽  
J. H. Kim ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Panax Ginseng ◽  
Yeast Extract ◽  
Adventitious Roots

scholarly journals The Arabidopsis mediator complex subunit 8 regulates oxidative stress responses

2021 ◽  
Author(s):  
Huaming He ◽  
Jordi Denecker ◽  
Katrien Van Der Kelen ◽  
Patrick Willems ◽  
Robin Pottie ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Stress Responses ◽  
Negative Regulator ◽  
Mediator Complex ◽  
Defense Gene Expression ◽  
A Genome ◽  
Oxidative Stress Responses

Abstract Signaling events triggered by hydrogen peroxide (H2O2) regulate plant growth and defense by orchestrating a genome-wide transcriptional reprogramming. However, the specific mechanisms that govern H2O2-dependent gene expression are still poorly understood. Here, we identify the Arabidopsis Mediator complex subunit MED8 as a regulator of H2O2 responses. The introduction of the med8 mutation in a constitutive oxidative stress genetic background (catalase-deficient, cat2) was associated with enhanced activation of the salicylic acid pathway and accelerated cell death. Interestingly, med8 seedlings were more tolerant to oxidative stress generated by the herbicide methyl viologen (MV) and exhibited transcriptional hyperactivation of defense signaling, in particular salicylic acid- and jasmonic acid-related pathways. The med8-triggered tolerance to MV was manipulated by the introduction of secondary mutations in salicylic acid and jasmonic acid pathways. In addition, analysis of the Mediator interactome revealed interactions with components involved in mRNA processing and microRNA biogenesis, hence expanding the role of Mediator beyond transcription. Notably, MED8 interacted with the transcriptional regulator NEGATIVE ON TATA-LESS, NOT2, to control the expression of H2O2-inducible genes and stress responses. Our work establishes MED8 as a component regulating oxidative stress responses and demonstrates that it acts as a negative regulator of H2O2-driven activation of defense gene expression.

scholarly journals Effects of Drying Methods on Antioxidant, Anti-Inflammatory, and Anticancer Potentials of Phenolic Acids in Lovage Elicited by Jasmonic Acid and Yeast Extract

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 662
Author(s):  
Urszula Złotek ◽  
Sławomir Lewicki ◽  
Anna Markiewicz ◽  
Urszula Szymanowska ◽  
Anna Jakubczyk
Keyword(s):  
Jasmonic Acid ◽  
Phenolic Acids ◽  
Yeast Extract ◽  
Syringic Acid ◽  
Epithelial Cell Line ◽  
Drying Methods ◽  
Simulated Digestion ◽  
Lipoxygenase Inhibition ◽  
Anti Inflammatory ◽  
Inhibition Ability

The study presents the effect of drying methods (traditional, convection, microwave, and freeze-drying) on the content and bioactivity (determined as antioxidative, anti-inflammatory, and antiproliferative potential) of potentially bioavailable fractions of phenolic acids contained in lovage elicited with jasmonic acid (JA) and yeast extract (YE) and in untreated control leaves. The highest amount of syringic acid was recorded in the convectionally dried lovage samples, while ethanolic extracts from lyophilized lovage had the highest content of protocatechuic and caffeic acids. The drying method significantly influenced the tested properties only in some cases. The traditional drying resulted in lower antioxidant potential, while convectional drying caused a reduction of the lipoxygenase inhibition ability of the samples after simulated digestion. Samples containing the control and elicited lovage leaves dried with convectional and traditional methods exhibited the highest cytotoxicity against a prostate cancer epithelial cell line.

scholarly journals Chrysolina herbaceamodulates jasmonic acid,cis-(+)-12-oxophytodienoic acid, (3R,7S)-jasmonoyl-l-isoleucine, and salicylic acid of local and systemic leaves in the host plantMentha aquatica

2011 ◽  
Vol 6 (2-3) ◽  
pp. 99-101 ◽  
Author(s):  
Andrea Occhipinti ◽  
Simon Atsbaha Zebelo ◽  
Andrea Capuzzo ◽  
Massimo Maffei ◽  
Giorgio Gnavi
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid

scholarly journals Restoration of Defective Cross Talk in ssi2 Mutants: Role of Salicylic Acid, Jasmonic Acid, and Fatty Acids in SSI2-Mediated Signaling

2003 ◽  
Vol 16 (11) ◽  
pp. 1022-1029 ◽  
Author(s):  
Pradeep Kachroo ◽  
Aardra Kachroo ◽  
Ludmila Lapchyk ◽  
David Hildebrand ◽  
Daniel F. Klessig
Keyword(s):  
Cell Death ◽  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Cross Talk ◽  
Pr Genes ◽  
Exogenous Application ◽  
Basal Level Expression ◽  
Sa Signaling ◽  
Spontaneous Cell Death

The Arabidopsis mutants ssi2 and fab2 are defective in stearoyl ACP desaturase, which causes altered salicylic acid (SA)- and jasmonic acid (JA)-mediated defense signaling. Both ssi2 and fab2 plants show spontaneous cell death, express PR genes constitutively, accumulate high levels of SA, and exhibit enhanced resistance to bacterial and oomycete pathogens. In contrast to constitutive activation of the SA pathway, ssi2 and fab2 plants are repressed in JA-mediated induction of the PDF1.2 gene, which suggests that the SSI2-mediated signaling pathway modulates cross talk between the SA and JA pathways. In this study, we have characterized two recessive nonallelic mutants in the ssi2 background, designated as rdc (restorer of defective cross talk) 2 and rdc8. Both ssi2 rdc mutants are suppressed in constitutive SA signaling, show basal level expression of PR-1 gene, and induce high levels of PDF1.2 in response to exogenous application of JA. Interestingly, while the rdc8 mutation completely abolishes spontaneous cell death in ssi2 rdc8 plants, the ssi2 rdc2 plants continue to show some albeit reduced cell death. Fatty acid (FA) analysis showed a reduction in 16:3 levels in ssi2 rdc8 plants, which suggests that this mutation may limit the flux of FAs into the pro-karyotic pathway of glycerolipid biosynthesis. Both rdc2 and rdc8 continue to accumulate high levels of 18:0, which suggests that 18:0 levels were responsible for neither constitutive SA signaling nor repression of JA-induced expression of the PDF1.2 gene in ssi2 plants. We also analyzed SA and JA responses of the fab2-derived shs1 mutant, which accumulates levels of 18:0 over 50% lower than those in the fab2 plants. Even though fab2 shs1 plants were morphologically bigger than fab2 plants, they expressed PR genes constitutively, showed HR-like cell death, and accumulated elevated levels of SA. However, unlike the ssi2 rdc plants, fab2 shs1 plants were unable to induce high levels of PDF1.2 expression in response to exogenous application of JA. Together, these results show that defective cross talk in ssi2 can be restored by second site mutations and is independent of morphological size of the plants, cell death, and elevated levels of 18:0.

scholarly journals Ultraviolet radiation enhances salicylic acid-mediated defense signaling and resistance to Pseudomonas syringae DC3000 in a jasmonic acid-deficient tomato mutant

2019 ◽  
Vol 14 (4) ◽  
pp. e1581560 ◽  
Author(s):  
Rocío Escobar Bravo ◽  
Gang Chen ◽  
Katharina Grosser ◽  
Nicole M. Van Dam ◽  
Kirsten A. Leiss ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Ultraviolet Radiation ◽  
Pseudomonas Syringae ◽  
Defense Signaling ◽  
Tomato Mutant

Salicylic acid but not jasmonic acid improved canola root response to salinity stress

Rhizosphere ◽  
2019 ◽  
Vol 9 ◽  
pp. 69-71 ◽  
Author(s):  
Salar Farhangi-Abriz ◽  
Tahereh Alaee ◽  
Alireza Tavasolee
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Salinity Stress ◽  
Root Response

Rice-Barnyard Grass Allelopathic Interaction: A Role of Jasmonic Acid and Salicylic Acid

2010 ◽  
Vol 113-116 ◽  
pp. 1782-1786 ◽  
Author(s):  
L.X. You ◽  
P. Wang
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Pearson Correlation ◽  
Signal Molecules ◽  
Rice Varieties ◽  
Barnyard Grass ◽  
Future Studies ◽  
Opposite Trend ◽  
Allelopathic Interaction

Exogenous jasmonic acid (JA) and salicylic acid (SA) can have an important effect on rice allelopathy. Currently, the role of endogenous JA and SA on rice-barnyard grass interaction is largely unknown. In this study, the levels of JA and SA in tissues and their correlation to rice allelochemicals inducing with barnyard grass are analyzed. Rice allelochemicals production was enhanced by coexistence with barnyard grass and allelochemicals of the allelopathic variety, PI312777 were generally higher than those of non-allelopathic variety, Liaojing9. Furthermore, JA contents of two rice varieties were generally greater in roots than in shoots, and differed clearly with tested times. The SA levels of PI312777 were generally higher than those of Liaojing9 in shoots but opposite trend in roots. The contents of total allelochemicals correlated positively with SA (Pearson correlation, r = 0.91, P < 0.001). These results indicate that as the phytohormones, JA and SA play a provable role in chemical communication between rice and barnyard grass and participate in rice-barnyard grass allelopathic interaction. Future studies should determine the signal molecules in root exudates of barnyard grass and their functions.

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