scholarly journals Micropropagation of Ruscus aculeatus L.

2018 ◽  
Vol 16 (1) ◽  
pp. 99-105
Author(s):  
Đinh Văn Khiêm ◽  
Hoàng Văn Cương ◽  
Nguyễn Thị Thanh Hằng ◽  
Phan Xuân Huyên ◽  
Nguyễn Thị Phượng Hoàng
Keyword(s):  
Shoot Regeneration ◽  
Large Scale ◽  
Root Formation ◽  
Shoot Length ◽  
Coconut Water ◽  
Ex Situ ◽  
New Directions ◽  
Population Reinforcement ◽  
Growth Of Seedlings

Ruscus aculeatus L. is a small evergreen perennial species, is commonly used either as medicinal or ornamental plant thanks to its evegreen long-lasting branches. Currently, the species is endangered by population redutions due to habitat alteration, the lack of seed production caused by pollination failure and also because of the intense harvesting for medicical uses or for floral bouquets. Micropropagation opens new directions for its ex situ conservation, as well as to generate large scale material for natural population reinforcement or for commercial purposes. In this study, effects of BA, NAA and coconut water on shoot regeneration, IBA on root formation of Ruscus aculeatus L., and type of substrates on acclimatization and growth of seedlings were investigated. The results showed that ½MS medium supplemented with 4 mg/l BA, 0.5 mg/l NAA and 15% (v/v) coconut water, 30 g/l sucrose, and pH 5.8 was the most suitable for shoot regeneration of Ruscus aculeatus L. in vitro (20.2 shoots, shoot length of  6.0 cm). Root formation of Ruscus aculeatus L. was optimal on ½MS supplemented with 1.5 mg/l IBA, 30 g/l sucrose, and pH 5.8 (frequency of rooting 100%, shoot length of 1.9 cm with 16.66 roots per seedling). Finally, the sufficiently rooted plantlets were transferred to greenhouse for hardening. After 2 months, coconut fiber substrate was the most suitable for seedling growth and development (with survival rate of 85%, root number of 8.7 and shoot length of 7.6 cm). We have developed protocol for the rapid micropropagation of Ruscus aculeatus L. in order to create a large number of seedlings for commercial production.

scholarly journals The Stimulating Effects of Imazalil and Carbendazim Fungicides on in Vitro Propagation and ex Situ conservation of the Medicinal Balkan Range-Restricted Sideritis Raeseri Boiss & Heldr. Subsp. Raeseri

2019 ◽  
Vol 10 ◽  
pp. 1752-1763 ◽  
Author(s):  
Virginia Sarropoulou ◽  
Eleni Maloupa
Keyword(s):  
Large Scale ◽  
Ex Situ Conservation ◽  
Germination Rate ◽  
Shoot Multiplication ◽  
Shoot Length ◽  
Ex Situ ◽  
Root Number ◽  
Short Period ◽  
Shoot Number

Sideritis raeseri Boiss & Heldr. subsp. raeseri, known in Greece as Mountain tea of Parnassus or Velouchi is a range restricted medicinal plant of the Balkan peninsula. Conventional propagation methods did not allow the mass production of plant material in a short period of time due to both low seed germination rate and rooting of cuttings. Therefore, the aim of this study was to establish a reliable, reproducible and efficient regeneration protocol for mass and large-scale micropropagation, germplasm and ex situ conservation of S. raeseri Boiss & Heldr. using Imazalil and Carbendazim fungicides. After 9 weeks, 2.5-10 mg/l Imazalil stimulated root length by 1 cm but diminished root number and rooting percentage. Optimum shoot number (5.58), shoot length (24.91 mm), shoot multiplication (100%), root number (20.63) and rooting (66.67%) were recorded with 0.5 mg/l kinetin (KN) + 0.05 mg/l ?-napthaleneacetic acid (NAA) (Imazalil-free). After 4 ½ weeks, 1 mg/l Carbendazim + 0.5 mg/l 6-benzyladenine (BA) yielded 5.77 shoots/explant, 16.1 mm shoot length and 100% shoot multiplication. However, Carbendazim did not result in rooting. The ex vitro survival percentage of rooted shoot-tip explants derived from Imazalil experiment was 95%. Carbendazim proved useful in shoot proliferarion and Imazalil in root elongation of S. raeseri Boiss & Heldr. subsp. raeseri micropropagation system.  

scholarly journals EFFECT OF COCONUT WATER AND ACTIVATED CHARCOAL ON SEED GERMINATION IN AN ENDEMIC ORCHID RHYNCHOSTYLIS RETUSA BLUME.

2017 ◽  
Vol 4 (2) ◽  
pp. 147-150
Author(s):  
Nisha Raj S
Keyword(s):  
Seed Germination ◽  
Activated Charcoal ◽  
In Vitro Propagation ◽  
Large Scale ◽  
Coconut Water ◽  
Rooting Medium ◽  
The Individual ◽  
Germination And Growth ◽  
Growth Of Seedlings

The present study on in vitro seed germination of Rhynchostylis retusa obtained on nutrient medium made clear pathway for the in vitro propagation of orchids by seed culture. The presence of coconut water in the medium enhanced seed germination and growth of seedlings. For enhancing the growth of seedlings further the individual seedlings were cultured on MS medium supplemented with 3 mg/l BAP and 1g/l activated charcoal. These seedlings were sub cultured on rooting medium (IBA) supplemented with activated charcoal. The plantlets were transferred to soil with 90% success. Hence the present investigation made possible for the large scale multiplication of R.retusa and thus to conserve this endemic orchid.

scholarly journals Improvement of micropropagation protocol of Phalaenopsis sp. for the method of direct shoot regeneration from nodes of floral spikes

2012 ◽  
pp. 141-150
Author(s):  
Marija Markovic ◽  
Milos Tanasic ◽  
Nevena Stojic ◽  
Radivoje Bulatovic ◽  
Marta Jovic ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Coconut Water ◽  
Soy Flour ◽  
Multiplication Rate ◽  
Direct Shoot Regeneration ◽  
Medium Components ◽  
Cultivation In Vitro ◽  
Direct Shoot

This paper succesfully investigated the possibility of modification of the micropropagation protocol of Phalaenopsis sp. with an aim to simplify the procedure and reduce the costs. The obtained results show that some medium components can be succesfully omitted (coconut water, glutamine, 2-morpholinoethanesulfonic acid) and some of them (peptone) can be replaced with a cheaper constituent (soy flour) while preserving the quality of the obtained microplants. The multiplication rate was 7,6 shoots per explant after the period of 150 days of cultivation in vitro. On the same medium 60% of explants were rooted and roots were mostly well developed.

scholarly journals In vitro regeneration performance of Corchorus olitorius

1970 ◽  
Vol 8 (1) ◽  
pp. 1-6 ◽  
Author(s):  
M Hoque ◽  
KM Nasiruddin ◽  
GKMN Haque ◽  
GC Biswas
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Root Formation ◽  
Plantlet Regeneration ◽  
Ms Medium ◽  
Corchorus Olitorius ◽  
The Media ◽  
Regenerated Plantlets

The experiment was conducted during May to December 2008 in the Biotechnology Laboratory of Bangladesh Agricultural University, Mymensingh to observe the callus induction, regeneration potentiality and to establish a suitable in vitro plantlet regeneration protocol of Corchorus olitorius. MS medium supplemented with different phytohormone concentrations and combinations were used to observe the callus induction, shoot regeneration and root formation ability of the cotyledon with attached petiole derived explant of three genotypes viz. O-9897, O-72 and OM-1. The highest callus induction (92.85%) was observed in O-9897 followed by O-72 (82.14%) in the MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. Genotype O-9897 in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA produced the highest percentage of shoot regenerants (83.33%) followed by O-72 (75.00%) in the media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. The root formation from regenerants was the best on halfstrength of MS media supplemented with 0.6 mg/L IBA in genotype O-9897 (45.00%). The in vitro regenerated plantlets from the genotypes O-9897 could be established in the field. Therefore, the genotypes O-9897 of C. olitorius in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA could be used for callus induction and shoot regeneration. Keywords: Regeneration; Phytohormone; Corchorus olitorius DOI: 10.3329/jbau.v8i1.6390J. Bangladesh Agril. Univ. 8(1): 1-6, 2010

scholarly journals In vitro ROOTING OF TENERA HYBRID OIL PALM (Elaeis guineensis Jacq.) PLANTS1

2018 ◽  
Vol 41 (4) ◽  
Author(s):  
Marlúcia Souza Pádua ◽  
Raíssa Silveira Santos ◽  
Luciano Vilela Paiva ◽  
Vanessa Cristina Stein ◽  
Luciano Coutinho Silva
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Oil Palm ◽  
Somatic Embryos ◽  
Large Scale ◽  
Elaeis Guineensis ◽  
Germination Percentage ◽  
Shoot Length ◽  
Indole Butyric Acid

ABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used to propagate oil palm is seed germination, but success is limited by long time requirements and low germination percentage. An alternative for large-scale propagation of oil palm is the biotechnological technique of somatic embryogenesis. The rooting of plants germinated from somatic embryos is a difficult step, yet it is of great importance for later acclimatization and success in propagation. The aim of this study was to evaluate the effect of the auxins indole acetic acid (IAA) and indole butyric acid (IBA) on the rooting of somatic embryos of Tenera hybrid oil palm. Plants obtained by somatic embryogenesis were inoculated in modified MS medium with 10% sucrose and 0.6% agar and supplemented with IAA or IBA at concentrations of 5 µM, 10 µM, and 15 µM, and the absence of growth regulators. After 120 days, the presence of roots, root type, length of the longest root, number of roots, number of leaves, and shoot length were analyzed. Growth regulators were favorable to rooting; plants cultivated with IBA growth regulator at 15 µM showed higher rooting percentage (87%) and better results for the parameters of number of roots (1.33) and shoot length (9.83).

scholarly journals Propagation of Dendrobium aggregatum by green capsule culture

Lankesteriana ◽  
2013 ◽  
Author(s):  
S. Vijayakumar ◽  
G. Rajalkshmi ◽  
K. Kalimuthu
Keyword(s):  
Root Formation ◽  
Coconut Water ◽  
Immature Embryos ◽  
Ms Medium ◽  
Medium Supplement ◽  
In Vitro Shoots ◽  
Immature Seeds ◽  
Benzyl Amino Purine ◽  
Number Of Shoots

An efficient protocol for propagation of Dendrobium aggregatum using the axenic immature seeds, derived from green capsule, was developed. The immature embryos from 120 days old capsules after pollination were germinated on Murashige and Skoog (MS) medium supplement with various concentration of BAP alone or in combination with NAA along with coconut water, and the same media were used for induction, multiplication, elongation and rooting in vitro shoots. MS medium with the addition of 3% sucrose 1.5 mg L-1 Benzyl amino purine (BAP) and 15% coconut water (CW) favoured the higher rate of germination, more number of protocorm like bodies, production of maximum number of shoots, elongation of shoots, as well as root formation. During acclimatization, 95% of the plantlets survived after one month.

scholarly journals Optimization of microclonal propagation in vitro of oregano (Origanum vulgare)

2018 ◽  
Vol 26 (2) ◽  
pp. 98-102 ◽  
Author(s):  
A. V. Fokina ◽  
T. M. Satarova ◽  
V. T. Smetanin ◽  
N. I. Kucenko
Keyword(s):  
Medicinal Plants ◽  
Root System ◽  
Root Formation ◽  
System Development ◽  
Practical Experience ◽  
Shoot Length ◽  
Herbaceous Plant ◽  
Human Immune System ◽  
Origanum Vulgare

Medicinal plants are important objects for botany, systematics and plant geography research, as well as physiology, pharmacology, and biotechnology. Medicinal plants from the Lamiaceae family are being intensively studied for medical and pharmacological reasons. This family also includes the medicinal herbaceous plant oregano (Origanum vulgare L.), known from ancient times for its antimicrobial and antifungal properties, the ability to strengthen the human immune system, and to improve the general state of an organism. At present, the study of its antimicrobial, antifungal, insecticidal, anticoagulant, antitumor, therapeutic and many other properties is being actively continued. Due to the relevance of the development of the principles of O. vulgare micropropagation in vitro and the undeveloped conditions and methods of its cultivation, the aim of this work was to optimize microclonation in vitro of oregano via the activation of auxiliary buds. The research tasks were to test the ability of auxiliary buds to be activated depending on the localization on the donor shoot internodes and to intensify the root formation of cuttings through medium content optimization. The influence of the location of the auxiliary buds on donor shoots on their activation in vitro was studied on such indicators as the length of newly formed shoots, the number of nodules per one newly formed shoot, and the number of newly formed shoots per one bud. In plant microclonal propagation, the stage of root formation is very important for further adaptation in soil. Practical experience has shown that for the effective adaptation of oregano in soil, the length of the root system for cuttings should be 1.5–2.0 cm, the degree of root system development – 4–5 points under shoot length of 3–5 cm. The study of peculiarities of oregano microclonal propagation via activation of auxiliary buds has allowed us to optimize the stage of explant selection for cutting and the formation of cuttings’ roots. It has been established that for optimal length, the number of nodules of the newly formed shoots and the number of newly formed shoots, the first internode, located on the top of a parent shoot, as well as the third to fifth ones are more suitable. For rooting oregano cuttings, the optimal medium on the ratio of length and density of root system and on shoot length is the nutrient one containing half of the macro-, microsalts and vitamins on Murashige-Skoog, 20 g/l sucrose and 0.75 mg/l kinetin.

scholarly journals In vitro Propagation of Bitter Gourd (Momordica charantia L.) from Nodal and Root Segments

1970 ◽  
Vol 19 (1) ◽  
pp. 45-52 ◽  
Author(s):  
M.A.Z. Al Munsur ◽  
M.S. Haque ◽  
K.M. Nasiruddin ◽  
M.S. Hossain
Keyword(s):  
Key Words ◽  
Shoot Regeneration ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Momordica Charantia ◽  
Shoot Length ◽  
Bitter Gourd ◽  
Nodal Segments ◽  
Root Segments

Explants of nodal and root segments of bitter gourd were cultured on MS supplemented with various concentrations of BAP in combination with either 2,4-D or NAA. Nodal segments produced the highest percentage (93.75) of callus in MS supplemented with 1.0 mg/l 2,4-D and 1.0 mg/l BAP whereas, root segments produced the highest (85.00%) callus in 0.6 mg/l NAA and 2.5 mg/l BAP combination. A combination of 1.0 mg/l 2,4-D and 1.0 mg/l BAP exhibited 75.00% shoot regeneration from nodal segments. The highest shoot length (5.15 cm) was recorded with 2.5 mg/l BAP and 0.2 mg/l IAA from nodal segments. No sign of regeneration of shoot was found from root segments in any of the combinations. However, some combinations produced only roots.  Key words: In vitro propagation, bitter gourd, nodal segments, Root segments D.O.I. 10.3329/ptcb.v19i1.4916 Plant Tissue Cult. & Biotech. 19(1): 45-52, 2009 (June)

Adventitious shoot regeneration from dormant buds of persimmon (Diospyros kaki Thunb.) cv. Hachiya

2011 ◽  
Vol 28 (2) ◽  
pp. 113-118 ◽  
Author(s):  
Zuzana Kochanová ◽  
Naci Onus ◽  
Ján Brindza
Keyword(s):  
Shoot Regeneration ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Hormone Treatment ◽  
Mediterranean Area ◽  
Shoot Length ◽  
Diospyros Kaki ◽  
Adventitious Shoot Regeneration ◽  
Significant Difference

Adventitious shoot regeneration from dormant buds of persimmon (Diospyros kakiThunb.) cv. HachiyaThe effects of plant hormones 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) on adventitious shoot regeneration from dormant persimmon buds were studied. The object of the study was the persimmon cultivar Hachiya, one of the most important persimmon cultivars in the Mediterranean area and Asia. Shoot regeneration was evaluated 4, 6 and 8 weeks after initiating the hormone treatment. Average shoot length was measured after 8 weeks and was evaluated by LSD test. Except for the media without hormone supplement, there was a statistically significant difference among average values of shoot length of plants, grown on the tested media. The increase in BAP had an effect on shoot regeneration that was significant and more pronounced with the addition of IBA, especially to the MS (1/2 N) medium. The highest value of shoot regeneration (98%) was obtained on medium MS (1/2 N), supplemented with 5 μmol l-1BAP and 1 μmol l-1IBA, with the highest average shoot length 23.69 mm, measured 8 weeks after the experiment initiation. The results indicate that adventitious shoots can be successfully produced in persimmon cv. Hachiya, especially with the supplement of hormone BAP, which, according to our results, plays an important role in persimmonin vitroregeneration.

scholarly journals Organic growth supplement stimulants for in vitro multiplication of Cymbidium pendulum (Roxb.) Sw.

2012 ◽  
Vol 39 (No. 1) ◽  
pp. 47-52 ◽  
Author(s):  
Saranjeet. Kaur ◽  
K.K. Bhutani
Keyword(s):  
Root Formation ◽  
In Vitro Regeneration ◽  
Root Hairs ◽  
Coconut Water ◽  
Regeneration Frequency ◽  
Organic Growth ◽  
Growth Supplement ◽  
Healthy Growth ◽  
Number Of Shoots

The aim of this study was to establish protocol for in vitro regeneration and multiplication of Cymbidium pendulum through protocorms, as explants using organic growth supplements such as banana homogenate, coconut water, and peptone. The effect of growth supplements was tested on multiplication (neo-formation of secondary protocorms from primary protocorms) of protocorms, development of maximum number of shoots and early plantlets in M medium (Mitra medium). Though the explants regenerated in the absence or presence of growth adjuncts in the medium; the regeneration frequency was found significantly higher in organic growth supplement-enriched medium than control. The protocorm segments (primary) regenerated into protocorm-like bodies (secondary protocorms) which eventually differentiated into shoots; no intervening callus stage was observed. Among the treatments, the highest regeneration frequency, robust shoots and root formation was recorded in banana homogenate (50 g/l); the roots were lengthy, thick, and covered all over the surface by root hairs. Higher concentration of banana homogenate (75 g/l) proved detrimental for the survival of cultures; the protocorm-like bodies showed necrosis and they perished soon after. Coconut water (10%) and peptone (2 g/l) supplemented medium proved optimum for profuse multiplication of protocorm-like bodies (PLBs) their early growth into plantlets. The treatments with organic growth supplements gave better and early plantlets as compared to control. Among these tested organic growth supplements, peptone (2 g/l) and coconut water (10%) proved beneficial for multiplication of regenerants, maximum number of shoots formation and early plantlet development; whereas banana homogenate (50 g/l) favoured the highest regeneration frequency and healthy growth of plantlets.

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