scholarly journals Optimization of microclonal propagation in vitro of oregano (Origanum vulgare)

2018 ◽  
Vol 26 (2) ◽  
pp. 98-102 ◽  
Author(s):  
A. V. Fokina ◽  
T. M. Satarova ◽  
V. T. Smetanin ◽  
N. I. Kucenko
Keyword(s):  
Medicinal Plants ◽  
Root System ◽  
Root Formation ◽  
System Development ◽  
Practical Experience ◽  
Shoot Length ◽  
Herbaceous Plant ◽  
Human Immune System ◽  
Origanum Vulgare

Medicinal plants are important objects for botany, systematics and plant geography research, as well as physiology, pharmacology, and biotechnology. Medicinal plants from the Lamiaceae family are being intensively studied for medical and pharmacological reasons. This family also includes the medicinal herbaceous plant oregano (Origanum vulgare L.), known from ancient times for its antimicrobial and antifungal properties, the ability to strengthen the human immune system, and to improve the general state of an organism. At present, the study of its antimicrobial, antifungal, insecticidal, anticoagulant, antitumor, therapeutic and many other properties is being actively continued. Due to the relevance of the development of the principles of O. vulgare micropropagation in vitro and the undeveloped conditions and methods of its cultivation, the aim of this work was to optimize microclonation in vitro of oregano via the activation of auxiliary buds. The research tasks were to test the ability of auxiliary buds to be activated depending on the localization on the donor shoot internodes and to intensify the root formation of cuttings through medium content optimization. The influence of the location of the auxiliary buds on donor shoots on their activation in vitro was studied on such indicators as the length of newly formed shoots, the number of nodules per one newly formed shoot, and the number of newly formed shoots per one bud. In plant microclonal propagation, the stage of root formation is very important for further adaptation in soil. Practical experience has shown that for the effective adaptation of oregano in soil, the length of the root system for cuttings should be 1.5–2.0 cm, the degree of root system development – 4–5 points under shoot length of 3–5 cm. The study of peculiarities of oregano microclonal propagation via activation of auxiliary buds has allowed us to optimize the stage of explant selection for cutting and the formation of cuttings’ roots. It has been established that for optimal length, the number of nodules of the newly formed shoots and the number of newly formed shoots, the first internode, located on the top of a parent shoot, as well as the third to fifth ones are more suitable. For rooting oregano cuttings, the optimal medium on the ratio of length and density of root system and on shoot length is the nutrient one containing half of the macro-, microsalts and vitamins on Murashige-Skoog, 20 g/l sucrose and 0.75 mg/l kinetin.

scholarly journals EFFECT OF ETIOLATION AND MEDIUM COMPOSITION ON RHYZOGENESIS OF APPLE ROOTSTOCK MICROPLANTS IN IN VITRO CULTURE

2018 ◽  
Vol 63 (3) ◽  
pp. 339-349
Author(s):  
V. A. Shaporeva ◽  
A. A. Zmushko ◽  
E. V. Kolbanova
Keyword(s):  
In Vitro Culture ◽  
Root System ◽  
Root Formation ◽  
System Development ◽  
Medium Composition ◽  
Apple Rootstock ◽  
Root System Development ◽  
Positive Effect

It was established that for in vitro rhyzogenesis of apple rootstock 106-13 it is better to use medium with 1/2 MS macro- and microsalts supplemented with IBA in concentration of 0.5 mg/l without use of etiolation (the yield of rooted microplants was 90.0 % with a coefficient of root system development – 1.14 ± 0.18). For in vitro rhyzogenesis of apple rootstock 54-118, it is better to use medium with 1/3 MS macro and microsalts, supplemented with IBA in concentration of 1.0 mg/l without etiolation (the yield of rooted microplants was 85.35 % with a coefficient of root system development – 0.53 ± 0.12). The positive effect of etiolation on root system growth depended on cultivar. For rootstock 54-118, the use of etiolation stimulated the growth of roots on medium with 1/3 MS macro- and microsalts combined with IBA in concentration of 1.0 mg/l (until the end of subculture). Use of etiolation inhibited the root system growth of rootstock 106-13 microplants until the end of subculture both on medium with 1/2 MS macro- and microsalts and on medium with 1/3 MS macro- and microsalts, but it stimulated root formation of rootstock 106-13. However, use of 7 days etiolation at stage of in vitro rhyzogenesis of rootstocks 106-13 and 54-118 is not expedient, because it does not stimulate the increase in the number of rooted microplants at the end of subculture.

scholarly journals Desenvolvimento de angelônia em vaso, sob doses de adubo de liberação lenta

2018 ◽  
Vol 24 (4) ◽  
pp. 387-392
Author(s):  
Mara Cíntia Winhelmann ◽  
Aquélis Armiliato Emer ◽  
Marília Tedesco ◽  
Priscila Paris ◽  
Claudimar Sidnei Fior ◽  
...  
Keyword(s):  
Root System ◽  
Chlorophyll A ◽  
Native Species ◽  
Slow Release ◽  
Polynomial Regression ◽  
Quadratic Equation ◽  
Shoot Length ◽  
Potted Plants ◽  
Number Of Stems

Angelonia integerrima is a native species that has ornamental potential, being cultivated in flowerbeds, flowerpots, pots, or even used as a complement in floral arrangements. Knowledge of mineral nutrition is required for the cultivation of the species, and slow release fertilization have become widespread in recent years, with a lower risk of toxicity to crops, an economy in relation to complementary fertilizers and may be an alternative to cultivation of potted plants. The aim of this study was to evaluate the development of Angelonia integerrima Sprengel in pot under slow release fertilization doses. Acclimatized seedlings with approximately 1 cm height, from in vitro germinated seeds were transplanted into 1 L pots containing substrate composted Pinus bark. Five doses of Basacote ® 9M slow release fertilizer, formulation 16-8-12 (N - P2O5 - K2O), 9 months: control - 0 g L-1, 2.5 g L-1, 5.0 g L-1, 7.5 g L-1 and 10.0 g L-1. The plants were grown in a greenhouse under drip irrigation. The shoot length, length of the largest inflorescence, number of stems, diameter of the largest stem, mean length of the stems, number of open flowers, fruits and senescent flowers, chlorophyll a, b and total, mass of dry matter of shoot and root system were evaluated after 286 days. The experimental design was completely randomized, with four replicates of six pots per plot. Data were submitted to analysis of variance (ANOVA) followed by polynomial regression by SigmaPlot 11.0. It was possible to adjust a quadratic equation for the variables: shoot length (with maximum response point in 7.2 g L-1), mean length of stems (6.7 g L-1), diameter of the largest stem and the root system (5.9 g L-1), number of open flowers, buds, senescent flowers and total flowers (7.7 g L-1). For the variables, number of stems, number of fruits, chlorophyll a, b and total did not differ between treatments. The slow releaser fertilizer indicated dose for the cultivation of the potted species is 7.2 g L-1.

scholarly journals Germination of seeds of medicinal plants Origanum vulgare L. and Salvia officinalis L. in vitro

2021 ◽  
Vol 102 (2) ◽  
pp. 23-29
Author(s):  
Symbat Dossymbetova ◽  
◽  
Aigul Amirova ◽  
Taira Kurbangaliyeva ◽  
Khalima Abdresh ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Animal Feed ◽  
Folk Medicine ◽  
Salvia Officinalis ◽  
In Vitro Cultivation ◽  
Origanum Vulgare ◽  
Optimal Method ◽  
Salvia Officinalis L ◽  
Developmental Features

The problem of biodiversity conservation and rational use of natural resources remains one of the most pressing problems for countries around the world. To maintain the stability of biological resources a sufficiently developed basis for their reproduction is required, which, in turn requires a preliminary study of the developmental features of individual organisms both in the natural environment and during their introduction into the cultural environment. A special place among the spice plants is occupied by the Lamiaceae Lindl. family. It is rich in medicinal and essential oil plants, which are widely used in family medicine, as well as in folk medicine and food industry, perfumery and cosmetic industries. Such plants include Origanum vulgare and Salvia officinalis. In addition, both plant species are added as phytobiotics to animal feed. The article deals with sterilization of seeds of medicinal plants Origanum vulgare L. and Salvia officinalis L. and the effect of sterilization methods on seed germination. For in vitro cultivation an aseptic, undamaged pure culture was obtained from the original plant material, and the most optimal method for sterilizing the seeds of these medicinal plants was chosen.

scholarly journals Micropropagation of dendrobium heterocarpum lindl.

2018 ◽  
Vol 16 (1) ◽  
pp. 127-135
Author(s):  
Dang Thi Tham ◽  
H’Yon Nie Bing ◽  
Nguyen Thi Thanh Hang ◽  
Dinh Van Khiem ◽  
Nong Van Duy ◽  
...  
Keyword(s):  
Endangered Species ◽  
Growth And Development ◽  
Root Formation ◽  
Shoot Length ◽  
Ms Medium ◽  
Orchid Species ◽  
Rapid Multiplication ◽  
Root Regeneration ◽  
Near Future

Dendrobium heterocarpum Lindl. is an endangered species which is currently used as ornamental pot plant for its beautiful flowers. An increase in collection for trade or any other purposes may lead to a dramatic decrease in the population of this species, thus becoming rare or endangered species in the near future. In this study, effects of plant growth regulators (BA, NAA, IAA, IBA, TDZ) and natural supplements (carrot, potato, and banana extracts) on protocorm like bodies (PLBs) formation; growth and development of shoot; and root regeneration of D. heterocarpum Lindl. as well as type of substrates on acclimatization and growth of seedlings were investigated. The results showed that PLBs formation was optimal on MS medium supplemented with 2.0 mg/L BA and 1.0 mg/L NAA (7.11 PLBs/explant; PLBs formation percentage of 68.9%) or MS medium supplemented with 1 mg/L TDZ and 0.5 mg/L NAA (7.29 PLBs/explant; PLBs formation percentage of 75.53%). For subculture, MS medium supplemented 1.5 mg/L BA and 60 g/L banana extract (22.40 shoots/explant; shoot length of 2 cm) was the most suitable for shoot regeneration and growth. Additionally, root formation was the most suitable on ½ MS medium supplemented with 1 mg/L NAA (4.4 roots/shoot; root length of 3.12 cm; root formation of 95.56%). Finally, the sufficiently rooted plantlets were transferred to greenhouse for hardening. After 60 days, coconut fiber substrate was the most suitable for seedling growth and development (with survival rate of 97.78%, root number of 5 and shoot length of 3.4 cm). The results of propagation in vitro Dendrobium heterocarpum Lindl. contribute to conservation and sustainable development as well as towards the rapid multiplication of seedlings for commercial commercialization of this wild orchid species.

scholarly journals In vitro cytotoxicity of allelopathic plants Adonis vernalis L. Origanum vulgare ssp. vulgare L. and Nepeta nuda subsp. nuda

2018 ◽  
Vol 5 (1) ◽  
pp. 64-69
Author(s):  
Vanya Koleva ◽  
Asya Dragoeva ◽  
Zheni Stoyanova ◽  
Zhenia Yordanova ◽  
Selime Ali ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Medicinal Plants ◽  
Cell Line ◽  
Hepatoma Cell ◽  
Water Extract ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Origanum Vulgare ◽  
Human Hepatoma Cell

AbstractMedicinal plants produce various secondary metabolites as a part of their chemical defence and survival in nature. These compounds have a wide range of biological activities. Nowadays, medicinal plants are used as source of allelochemicals and new effective anticancer agents. Our previous studies revealed allelopathic potential of water extracts of Adonis vernalis L. (Ranunculaceae), Origanum vulgare ssp. vulgare L. and Nepeta nuda subsp. nuda (Lamiaceae). Present study aimed to evaluate the effect of the same extracts in vitro on human hepatoma cell line SK-HEP-1. Cell proliferation/viability was assessed using Premixed WST-1 Cell Proliferation Reagent.Adonis water extract (1.83mg/ml) had notable negative influence on cancer cell line tested. Oregano (3.5 mg/ml) also exerted negative effect, but to a lesser degree. On the contrary, nepeta water extract (6.59 mg/ml) had an opposite effect, stimulating cell proliferation. One possible explanation could be the type of extraction: after treatment with nepeta methanol extract (6.59 mg/ml) cell viability was significantly reduced.In conclusion, Adonis vernalis and Nepeta nuda subsp. nuda possess metabolites with growth inhibitory effect on human hepatoma cell line SK-HEP-1. Further research is needed to clarify biological activity of lower concentrations which are appropriate to enable the design of new anticancer drugs.

scholarly journals Micropropagation of Ruscus aculeatus L.

2018 ◽  
Vol 16 (1) ◽  
pp. 99-105
Author(s):  
Đinh Văn Khiêm ◽  
Hoàng Văn Cương ◽  
Nguyễn Thị Thanh Hằng ◽  
Phan Xuân Huyên ◽  
Nguyễn Thị Phượng Hoàng
Keyword(s):  
Shoot Regeneration ◽  
Large Scale ◽  
Root Formation ◽  
Shoot Length ◽  
Coconut Water ◽  
Ex Situ ◽  
New Directions ◽  
Population Reinforcement ◽  
Growth Of Seedlings

Ruscus aculeatus L. is a small evergreen perennial species, is commonly used either as medicinal or ornamental plant thanks to its evegreen long-lasting branches. Currently, the species is endangered by population redutions due to habitat alteration, the lack of seed production caused by pollination failure and also because of the intense harvesting for medicical uses or for floral bouquets. Micropropagation opens new directions for its ex situ conservation, as well as to generate large scale material for natural population reinforcement or for commercial purposes. In this study, effects of BA, NAA and coconut water on shoot regeneration, IBA on root formation of Ruscus aculeatus L., and type of substrates on acclimatization and growth of seedlings were investigated. The results showed that ½MS medium supplemented with 4 mg/l BA, 0.5 mg/l NAA and 15% (v/v) coconut water, 30 g/l sucrose, and pH 5.8 was the most suitable for shoot regeneration of Ruscus aculeatus L. in vitro (20.2 shoots, shoot length of  6.0 cm). Root formation of Ruscus aculeatus L. was optimal on ½MS supplemented with 1.5 mg/l IBA, 30 g/l sucrose, and pH 5.8 (frequency of rooting 100%, shoot length of 1.9 cm with 16.66 roots per seedling). Finally, the sufficiently rooted plantlets were transferred to greenhouse for hardening. After 2 months, coconut fiber substrate was the most suitable for seedling growth and development (with survival rate of 85%, root number of 8.7 and shoot length of 7.6 cm). We have developed protocol for the rapid micropropagation of Ruscus aculeatus L. in order to create a large number of seedlings for commercial production.

scholarly journals Auxin Prompted Improved Micropropagation Protocol of Picrorhiza kurroa: An Endangered Medicinal Plant

1970 ◽  
Vol 19 (2) ◽  
pp. 161-167 ◽  
Author(s):  
Arif Jan ◽  
Phalisteen S ◽  
G.T. Thomas ◽  
A. S. Shawl
Keyword(s):  
Medicinal Plant ◽  
Culture Medium ◽  
Root Formation ◽  
Normal Development ◽  
Shoot Multiplication ◽  
Shoot Length ◽  
Picrorhiza Kurroa ◽  
Endangered Medicinal Plant ◽  
Mother Plants

In vitro shoot multiplication from nodal explants of Picrorhiza kurroa Royle ex. Benth. (Kour) was obtained on MS without cytokinin. Addition of NAA in the culture medium favours shoots as well as root formation. Among the various combinations of MS and B5 media, the average number of shoots per explant was (18.3) on MS supplemented with 0.6 mg/l NAA. Shoot length was maximum (5.8 cms) in MS augmented with 0.4 mg/l  NAA. The elongated shoots rooted within 15 - 25 days either on MS alone or supplemented with NAA (0.4 mg/l), IAA and IBA (0.1 - 0.5 mg/l). About 81.5% of the rooted plantlets survived acclimation and transferred to the greenhouse. All the in vitro derived plants at maturity displayed normal development as that of the mother plants at their maturity. Key words: Picrorhiza kurroa, Hardening, Medicinal plant, Propagation D.O.I. 10.3329/ptcb.v19i2.5433 Plant Tissue Cult. & Biotech. 19(2): 161-167, 2009 (December)

In Vitro Anti-Infective Screening Of Selected West African Medicinal Plants

Planta Medica ◽  
2016 ◽  
Vol 82 (05) ◽  
Author(s):  
VO Imieje ◽  
PS Fasinu ◽  
KO Ogbeide ◽  
NO Egiebor ◽  
A Falodun
Keyword(s):  
Medicinal Plants ◽  
West African
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