scholarly journals Enhanced bioremediation of crude oil polluted water by a hydrocarbon-degrading Bacillus strain immobilized on polyurethane foam

2020 ◽  
Vol 18 (3) ◽  
pp. 581-588
Author(s):  
Kieu Thi Quynh Hoa ◽  
Nguyen Vu Giang ◽  
Nguyen Thi Yen ◽  
Mai Duc Huynh ◽  
Nguyen Huu Dat ◽  
...  
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Immobilized Cells ◽  
Polluted Water ◽  
Contaminated Water ◽  
Bacillus Sp ◽  
Microbial Cells ◽  
Bacillus Strain ◽  
Enhanced Bioremediation ◽  
Marine Environmental

During the production and transportation of petroleum hydrocarbons, unsuitable operation and leakage may result in contamination of water and soil with petroleum hydrocarbons. Petroleum contamination causes significant marine environmental impacts and presents substantial hazards to human health. Bioremediation of contaminated water and soil is currently the effective and least harmful method of removing petroleum hydrocarbons from the environment. To improve the survival and retention of the bioremediation agents in the contaminated sites, microbial cells must be immobilized. It was demonstrated that immobilized microbial cells present advantages for degrading petroleum hydrocarbon pollutants compared to free suspended cells. In this study, the ability of a Bacillus strain (designed as Bacillus sp. VTVK15) to immobilize on PUF and to degrade crude oil was investigated.  The immobilized Bacilllus strain had the highest number (5.38 ± 0.12 Í 108 CFU/g PUF) and a maximum attachment efficiency of 92% on PUF after 8 days. Analysis by GC-MS revealed that both free and immobilized cells of Bacillus sp. VTVK15 were able to degrade 65 and 90% of the hydrocarbons in 2% (v/v) crude oil tested after 14 days, respectively. The results suggest the potential of using PUF-immobilized Bacillus sp. VTVK15 to bioremediate petroleum hydrocarbons in an open marine environment.

UV Induced Degradation of Crude Oil in Polluted Water Based on Analysis of Environmental Materials

2012 ◽  
Vol 534 ◽  
pp. 333-336 ◽  
Author(s):  
Hui Liu ◽  
Bing Sun ◽  
Zhi Yu Yan ◽  
Xiao Yu Tian
Keyword(s):  
Crude Oil ◽  
Fluorescence Spectra ◽  
Uv Light ◽  
Polluted Water ◽  
Contaminated Water ◽  
Saturated Hydrocarbons ◽  
Uv Light Irradiation ◽  
Dissolved Organic Matters ◽  
Synchronous Fluorescence Spectra ◽  
Environmental Materials

Analysis of environmental materials is very important for the environmental assessment, especially for the crude oil-contaminated water. This paper investigated the degradation of crude oil in the polluted water under the irradiation of UV light, and used the UV absorption, synchronous fluorescence spectra and gas chromatograph to analysis the changes of the environmental materials in the crude oil polluted water during the irradiation. All of the absorbance substances, fluorescent substances and saturated hydrocarbons in the crude oil polluted water decreased with the UV light irradiation. In addition, the dissolved organic matters in the seawater had significant influence on the photodegradation of crude oil.

Efficiency and Kinetics of Total Petroleum Hydrocarbons (TPHs) Removal from Crude Oil Polluted Arable Soil using Palm Bunch Ash and Tween 80

2021 ◽  
Author(s):  
Godwin James Udo ◽  
Nnanake-Abasi O. Offiong ◽  
Alfreda Nwadinigwe ◽  
Clement O. Obadimu ◽  
Aniedi E. Nyong ◽  
...  
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Tween 80 ◽  
Arable Soil ◽  
Total Petroleum Hydrocarbons ◽  
Kinetics Of ◽  
Palm Bunch

Biodegradation of different petroleum hydrocarbons by free and immobilized microbial consortia

2015 ◽  
Vol 17 (12) ◽  
pp. 2022-2033 ◽  
Author(s):  
Tiantian Shen ◽  
Yongrui Pi ◽  
Mutai Bao ◽  
Nana Xu ◽  
Yiming Li ◽  
...  
Keyword(s):  
Crude Oil ◽  
Petroleum Hydrocarbons ◽  
Microbial Consortia ◽  
Biodegradation Rate

The biodegradation rate of crude oil by semi-coke immobilized microbial consortia was higher than that by free microbial consortia.

Kinetics of nutrient enhanced crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2 isolated from Guwahati refinery, India

2016 ◽  
Vol 216 ◽  
pp. 548-558 ◽  
Author(s):  
Bobby Chettri ◽  
Arghya Mukherjee ◽  
James S. Langpoklakpam ◽  
Dhrubajyoti Chattopadhyay ◽  
Arvind K. Singh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Crude Oil ◽  
Oil Degradation ◽  
Bacillus Sp ◽  
Crude Oil Degradation ◽  
Kinetics Of

Application of nitrogen fixing bacteria and poultry droppings for enhanced bioremediation of crude oil polluted-soil

2021 ◽  
Vol 20 (1) ◽  
pp. 109-124
Author(s):  
V.C Wokem ◽  
E.D. Momoh
Keyword(s):  
Crude Oil ◽  
Niger Delta ◽  
Tropical Rainforest ◽  
Polluted Soil ◽  
Nitrogen Fixing ◽  
Delta Region ◽  
Nitrogen Fixing Bacteria ◽  
Niger Delta Region ◽  
Enhanced Bioremediation ◽  
Poultry Droppings

The exploration, production and refining of crude oil has led to severe environmental degradation in the oil producing communities of the Niger Delta region of Nigeria. Enhanced bioremediation of tropical rainforest soil artificially polluted with crude oil, bioaugmented with nitrogen fixing bacteria (NFB) and biostimulated with poultry droppings was carried out ex situ. Soil sample was collected at 15cm depth from tropical rainforest soil of the University of Port Harcourt, Nigeria. The NFB was isolated from roots of leguminous plant Arachis hypogea, identified as Nitrobacter species. Bioaugmentation by application of NFB served as option A, option B (biostimulation by application of poultry droppings), option C (No amendment) served as the control. Bioremediation was monitored for 28 days for interval of 14 days, and determined using the percentage ratio of total petroleum hydrocarbon (TPH) losses for each period to TPH at initial day (day zero). Results of total culturable heterotrophic bacterial (TCHB) counts showed that highest range in option B (1.9×104- 2.4×109Cfu/g) than in option A (7.8×106 -2.29×107Cfu/g) and C (6.75×106 -2.6×107Cfu/g) respectively. Similarly, hydrocarbon utilizing bacterial (HUB) counts had higher range in option B (1.20×105 - 1.9×107Cfu/g) than in option A (8.30×104 - 2.30×105Cfu/g) and option C control (4.3×104 −1.69×105 Cfu/g) respectively. Changes in physicochemical parameters during the study showed reductions in nitrate, phosphate and TPH in all the options expect pH which showed slight increase in option C (6.20-6.24). Characterization and identification for bacteria revealed the following HUB genera Pseudomonas, Citrobacter, Bacillus, Corynebacterium, Micrococcus, Klebsiella, Staphylocuccus and Nitrobacter). The percentage losses in TPH from gas chromatography (GC) results showed the following; option A (44.24%) option B (61.08%) and option C - control (27.28%) respectively. The results from this study showed that option B, the application of poultry droppings as biostimulant was more efficient than the application of NFB in enhanced bioremediation of crude oil polluted soil, hence the use of poultry droppings which is available as organic waste, eco-friendly and cost-effective is recommended asbiostimulant for enhanced bioremediation in environmental cleanup of crude oil impacted-sites of the Niger Delta region of Nigeria. Key Words: Bioremediation, Crude oil polluted-soil, Biostimulation, Bioaugumentation, Poultry droppings, Nitrogen fixing bacteria.

Chromatographic study of crude oil

2020 ◽  
pp. 47-50
Author(s):  
T.S. Idrisov ◽  
Keyword(s):  
Crude Oil ◽  
Aromatic Compounds ◽  
Petroleum Hydrocarbons ◽  
Chromatographic Analysis ◽  
Scientific Research ◽  
Chromatographic Study ◽  
Column Temperature ◽  
Tof Ms

The paper deals with the methods of preparation of crude oil samples and chromatographic analysis of petroleum hydrocarbons (alkanes, monoaromatic compounds, policyclic aromatic compounds), as well as the results of the analysis (chromatograms of the samples and concentrates of hydrocarbons). The analysis have been carried out on Shimadzu GC 2010-plus and PEGASUS 4D GCxGC TOF-MS chromatographs. In the analysis of alkanes, monoaromatic and policyclic aromatic compounds the column temperature comprised 60−330 оС, 40−120 оС and 70−300 оС correspondingly. Silicagel of 100−200 mehs size was used as a sorbent. Developed methods may be applied for the analytical purposes in petrochemistry and corresponding scientific research surveys as well.

NOVEL STRAINS OF MICROORGANISMS DEGRADING PETROLEUM HYDROCARBONS

2020 ◽  
pp. 311-313
Author(s):  
N. Tyschenko ◽  
D. Ivasenko ◽  
А. Kosov ◽  
D. Rybkin ◽  
Е. Lukjanova ◽  
...  
Keyword(s):  
Crude Oil ◽  
Bottom Sediments ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Biological Products

Novel strains of hydrocarbons degrading microorganisms were isolated from oil contaminated soil and bottom sediments and identified. Isolates oxidize components of crude oil in presence of oxygen and can be used as a part of commercial biological products for oil destruction.

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