Extremophiles as a Source of Biotechnological Products

Extremophile and extremozyme capabilities to uphold catalytic actions under extreme situations open up a varied array of biotechnological applications. Extremophiles are a rich supply of biocatalysts used for innumerable purposes. Bioactive molecules and enzymes isolated from organisms inhabiting risky environments being used in biological innovation pipelines and pharmaceutical have positive claims. The species biodiversity has favourable reservoir of the unexploited amalgams with biotechnological significance. Prospective solicitations of extremozymes, chiefly as catalysis of multistep progressions, quorum sensing, bioremediation, biofuel, biodiversity and prospecting, biomining, and genetic technology are explored. To boost the biotechnological uses of extremozymes, research and development efforts are needed to address hurdles such as extremophile culture, gene expression in host cells, and extremozyme bioprocessing. Extremophiles can be a resource for innovative biotechnological comprising industrial biotechnology, agriculture, medical, food, and environmental biotechnology.

A Pesticide Biopurification System: A Source of Biosurfactant-Producing Bacteria with Environmental Biotechnology Applications

Biosurfactants, a wide group of compounds produced by different microorganisms, generally have less toxicity and are more biodegradable than synthetic surfactants. Biosurfactant-producing bacteria can be found in contaminated environments, such as soils receiving pesticide applications constantly, or in pesticides treatment systems where microorganisms are adapted to biodegrading pesticides. Five pesticide-tolerant bacteria previously isolated from a pesticide biopurification system were evaluated as biosurfactant-producers. Pseudomonas rhodesiae C4, Rhodococcus jialingiae C8 and Pseudomonas marginalis C9 strains were positive in qualitative tests. Biosurfactant production by these strains using Bushnell-Haas medium with olive oil at 2% (w/v) was evaluated as emulsification index, oil displacement, droplet collapse test and surface tension. After 144 h, these strains showed a similar emulsification index of >55%. The two Pseudomonas (C4 and C9) strains showed lower superficial tension compared with Rhodococcus strain (C8)—34.47, 37.44 and 47.55 mN/m for strains C4, C9 and C8, respectively. The chemical characterization of the biosurfactants revealed the presence of glycolipids in P. rhodesiae (C4) and glycopeptides in P. marginalis (C9). The degradation of chlorpyrifos increased from 39.2% to 51.6% when biosurfactants produced by P.rhodesiae (C4) were added (10%) with respect to the control. Therefore, biopurification systems are a relevant source of biosurfactant-producing bacteria with environmental biotechnology applications.

Recovery and Recycling of Valuable Metals from Low-Grade Ores Using Microorganisms: A Brief Review

The demand for metals is ever increasing with the advancement of the industrialized world. But the global reserve high levels of ores are adjacent to decline. However, there exists there is a vast reserve of metals inferior ore, and other subsidiary sources. Low category ores as well as metal recovery conventional strategies such as pyrometallurgy, hydrometallurgy, etc., require strong and asset inputs that are often environmentally friendly pollution. Accordingly, there is required for the utilization of more coherent technologies to the recuperation of metals. The utilization of microbes to recovery metal ions is considered a unique key optimistic and revolutionary field of environmental biotechnology. The components of this method are disintegrated in an aqueous solution, which provides them more effective in addition, treatment, and convalescence. Recycling giant metals is also very important to prevent pollution and to prevent wastage of sources. Biological means are also used to easily recycle metals from their secondary sources. In this research, various approaches using microbes to recover giant metals from primary (low-grade ore) and secondary (electronic wastes) sources are discussed. Future prospects of utilizing microbes are also granted here.