scholarly journals SELECTION OF INDIGENOUS STRAINS (Paecilomyces lilacinus) PARASITIZE Meloidogyne spp. ISOLATED FROM BA RIA – VUNG TAU PROVINCE

2018 ◽  
Vol 55 (1A) ◽  
pp. 27
Author(s):  
Nguyen Thi Thuy Duong
Keyword(s):  
Forest Soils ◽  
Black Pepper ◽  
Paecilomyces Lilacinus ◽  
Egg Masses ◽  
Parasitizing Female ◽  
Meloidogyne Spp ◽  
Selection Of

Twenty two Paecilomyces lilacinus strains were isolated from forest soils and black pepper rhizospheres in Ba Ria –Vung Tau Province. The ability to degrade chitin of PB 3.3, PB 2.9, QT2, and QT5 strains was high. The ability to degrade casein of PB 1.3, PB 2.10, KL5, and KL6 strains was efficient. And then, these strains were parasitized females and egg masses of Meloidogyne spp. in vitro. In female parasitism test, the rates of parasitizing female nematodes reached more than 50 % after treating for 2 days. Four strains of PB 2.10, PB 1.3, KL6 and QT5 belonged to the first group achieved the highest parasitic (> 90 %) effects on female after 3 days of incubation. In egg masses parasitism test, three strains of PB 1.3, PB 2.10 and QT5 exhibited 83.33 %, 75 % and 75 % parasitism on egg masses after 11 days of incubation. The rates of parasitizing female were higher than egg masses. Three selected strains from the experiments were PB 1.3, PB 2.10 and QT5. 

scholarly journals Effect of Paecilomyces lilacinus on tomato plants and the management of root knot nematodes

2019 ◽  
Vol 17 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Shahriyar Ahmed ◽  
Mohammad Shahjahan Monjil
Keyword(s):  
Plant Pathology ◽  
Plant Growth ◽  
Growth Parameters ◽  
Research Work ◽  
Paecilomyces Lilacinus ◽  
Growth Reduction ◽  
Root Knot Nematodes ◽  
Tomato Plants ◽  
Egg Masses ◽  
Meloidogyne Spp

Effect of Paecilomyces lilacinus on tomato plant growth and the management of root knot nematodes in tomato was studied. The research work was conducted in Microbiology & Bio-control Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh and in Net-house of Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, during the period from July, 2016 to October, 2017. In net-house pot culture experiment, four different treatments were used viz., T1: Inoculation of egg masses (10 egg masses/plant) of Nematodes (Meloidogyne spp.), T2: Application of P. lilacinus, T3: Application of egg masses of Nematodes and P. lilacinus simultaneously, and T4: Control (non-treated). Spore suspension (10×105 Conc.) of P. lilacinus was mixed with the soil before transplantation and Meloidogyne spp. was inoculated on three days after transplantation. Application of P. lilacinus in soil enhanced the plant growth parameters of tomato plants. Inoculation of Meloidogyne spp. reduced plant growth and the reduction was increased with the increase of inoculum density of Meloidogyne spp. Maximum plant growth reduction was recorded when Meloidogyne spp. was inoculated alone. The maximum plant growth was recorded in case of application of P. lilacinus to soil. A high percentage (85%) of egg masses of Meloidogyne spp. was infected by P. lilacinus when applied together J. Bangladesh Agril. Univ. 17(1): 9–13, March 2019

scholarly journals EFFECTS OF EXTRACTS FROM LEAVES AND ROOTS OF Melaleuca cajuputi Powell ON NEMATODES CAUSING DISEASES ON Piper nigrum L. (BLACK PEPPER) IN VITRO

2019 ◽  
Vol 1 (34) ◽  
pp. 43-51
Author(s):  
Xuyen Hoang Le ◽  
Thai Quoc Nguyen ◽  
Truong Nhat Nguyen ◽  
Hoi Hoang Luu
Keyword(s):  
Rate Control ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Natural Death ◽  
Fusarium Spp ◽  
Phytophthora Spp ◽  
Melaleuca Cajuputi ◽  
Meloidogyne Spp ◽  
Leaves And Roots

This study evaluates the effects of different extracts from leaves (fresh and dry) and roots of melaleuca on  pathogens such as Meloidogyne spp., Phytophthora spp. and Fusarium spp. which affect pepper orchards in Hau Giang province, Vietnam. The results showed that the extracts increased the rate of death of a lethal nematode (Meloidogyne spp.) on pepper up to 100%, while the natural death rate (control) only reached to58% at 3 days after stocking in vitro, and the extracts from fresh leaves had the quickest response. However, extracts from both the melaleuca fresh leaves and roots were not effective on the development of all 11 strainsof Fusarium spp. and 5 strains of Phytophthora spp. which were tested after 3 days of inoculation. The results illustrated that the extracts of dried leaves was positive against the growth of 2 strains (18%), increased thegrowth of 8 strains (73%) and ineffective on 1  strain (9%) of Fusarium spp., and completely ineffective on all 5 strains of Phytophthora spp.

scholarly journals IDENTIFICATION AND MICROPROPAGATION OF VIRUS-FREE BLACK PEPPER GENOTYPES (Piper nigrum L.)

2020 ◽  
Vol 18 (1) ◽  
pp. 57
Author(s):  
Gleyce kelly Sousa Ramos ◽  
Oriel Filgueira de Lemos ◽  
Elisa Ferreira Moura Cunha ◽  
Alessandra De Jesus Boari ◽  
Danielle Pereira Mendonça ◽  
...  
Keyword(s):  
In Vitro Selection ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Multiplication Rate ◽  
Virus Infections ◽  
Major Constraint ◽  
Selection Of ◽  
Pepper Plants

Virus infections are a major constraint for black pepper cultivation (Piper nigrum L.), a vegetatively propagated crop; therefore, the in vitro selection of virus- free plants is an alternative to reestablish phytosanitary quality of pepper culture. The aim of this study was to select and micropropagate PYMoV virus-free pepper plants. ‘Apra’, ‘Bragantina’, ‘Cingapura’, ‘Clonada’, hybrids and ‘Kottanadan’ genotypes were used in the indexing process. From these, ‘Cingapura’, ‘Clonada’, hybrids and ‘Kottanadan’ were virus free, and only ‘Cingapura’ and ‘Clonada’ were selected for micropropagation. In the multiplication phase, ‘Cingapura’ genotype showed higher multiplication rate in relation to ‘Clonada’ in in vitro rooting and in acclimatization, response was similar in both genotypes. It was possible to identify virus-free genotypes of commercial interest, selecting them for micropropagation, and in this stage, influence of genotype is of great importance.

Clone selection of in vitro horseradish hairy root cultures

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
R Bertóti ◽  
Á Alberti ◽  
A Böszörményi ◽  
R Könye ◽  
T Horváth ◽  
...  
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Clone Selection ◽  
Selection Of

The Effects of Shock Vancomycin Concentrations on the Formation of Heteroresistance in Staphylococcus aureus

2020 ◽  
Vol 65 (9-10) ◽  
pp. 3-7
Author(s):  
V. V. Gostev ◽  
Yu. V. Sopova ◽  
O. S. Kalinogorskaya ◽  
M. E. Velizhanina ◽  
I. V. Lazareva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
In Vitro Selection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
High Concentration ◽  
Short Term ◽  
High Concentrations ◽  
Selection Of ◽  
Selection Of Resistance ◽  
Test Cultures

Glycopeptides are the basis of the treatment of infections caused by MRSA (Methicillin-Resistant Staphylococcus aureus). Previously, it was demonstrated that antibiotic tolerant phenotypes are formed during selection of resistance under the influence of high concentrations of antibiotics. The present study uses a similar in vitro selection model with vancomycin. Clinical isolates of MRSA belonging to genetic lines ST8 and ST239, as well as the MSSA (ATCC29213) strain, were included in the experiment. Test isolates were incubated for five hours in a medium with a high concentration of vancomycin (50 μg/ml). Test cultures were grown on the medium without antibiotic for 18 hours after each exposure. A total of ten exposure cycles were performed. Vancomycin was characterized by bacteriostatic action; the proportion of surviving cells after exposure was 70–100%. After selection, there was a slight increase in the MIC to vancomycin (MIC 2 μg/ml), teicoplanin (MIC 1.5–3 μg/ml) and daptomycin (MIC 0.25–2 μg/ml). According to the results of PAP analysis, all strains showed an increase in the area under curve depending on the concentration of vancomycin after selection, while a heteroresistant phenotype (with PAP/AUC 0.9) was detected in three isolates. All isolates showed walK mutations (T188S, D235N, E261V, V380I, and G223D). Exposure to short-term shock concentrations of vancomycin promotes the formation of heteroresistance in both MRSA and MSSA. Formation of VISA phenotypes is possible during therapy with vancomycin.

Wheat Germ Cell-Free Translation System as a Tool for In vitro Selection of Functional Proteins

2002 ◽  
Vol 5 (6) ◽  
pp. 473-480
Author(s):  
Bentham Science Publisher A.N. Alexandrov ◽  
Bentham Science Publisher V.Yu. Alakhov ◽  
Bentham Science Publisher A.I. Miroshnikov
Keyword(s):  
Germ Cell ◽  
Wheat Germ ◽  
In Vitro Selection ◽  
Translation System ◽  
Free Translation ◽  
Selection Of
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