Bone Repair in Periodontal Defect Using a Composite of Allograft and Calcium Sulfate (DentoGen) and a Calcium Sulfate Barrier

2011 ◽  
Vol 37 (2) ◽  
pp. 287-292 ◽  
Author(s):  
Ziv Mazor ◽  
Sachin Mamidwar ◽  
John L. Ricci ◽  
Nick M. Tovar*
Keyword(s):  
Connective Tissue ◽  
Periodontal Disease ◽  
Bone Defect ◽  
Calcium Sulfate ◽  
Bone Repair ◽  
Bone Allograft ◽  
Bone Defects ◽  
Defect Analysis ◽  
Freeze Dried ◽  
Time Point

Abstract Deep bone defects are caused by the progression of periodontal disease, which breaks down bone and connective tissue that hold teeth in place. In this case, a 37-year-old male patient presented a deep bone defect with advanced periodontal disease around an upper canine. Medical-grade calcium sulfate was mixed with demineralized freeze-dried bone allograft and used to repair and regenerate the defect. Analysis of the radiographs at the 5-month time point showed the bone had completely regenerated.

scholarly journals The Development of Gelatin/Hyaluronate Copolymer Mixed with Calcium Sulfate, Hydroxyapatite, and Stromal-Cell-Derived Factor-1 for Bone Regeneration Enhancement

Polymers ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1454 ◽  
Author(s):  
Yun-Liang Chang ◽  
Chia-Ying Hsieh ◽  
Chao-Yuan Yeh ◽  
Feng-Huei Lin
Keyword(s):  
Bone Regeneration ◽  
Bone Defect ◽  
Calcium Sulfate ◽  
Stromal Cell ◽  
Femoral Condyle ◽  
Bone Defects ◽  
Blood Analysis ◽  
Control Group ◽  
Defect Model ◽  
Stromal Cell Derived Factor

In clinical practice, bone defects still remain a challenge. In recent years, apart from the osteoconductivity that most bone void fillers already provide, osteoinductivity has also been emphasized to promote bone healing. Stromal-cell-derived factor-1 (SDF-1) has been shown to have the ability to recruit mesenchymal stem cells (MSCs), which play an important role in the bone regeneration process. In this study, we developed a gelatin–hyaluronate (Gel-HA) copolymer mixed with calcium sulfate (CS), hydroxyapatite (HAP), and SDF-1 in order to enhance bone regeneration in a bone defect model. The composites were tested in vitro for biocompatibility and their ability to recruit MSCs after material characterization. For the in vivo test, a rat femoral condyle bone defect model was used. Micro computed tomography (Micro-CT), two-photon excitation microscopy, and histology analysis were performed to assess bone regeneration. As expected, enhanced bone regeneration was well observed in the group filled with Gel-HA/CS/HAP/SDF-1 composites compared with the control group in our animal model. Furthermore, detailed blood analysis of rats showed no obvious systemic toxicity or side effects after material implantation. In conclusion, the Gel-HA/CS/HAP/SDF-1 composite may be a safe and applicable material to enhance bone regeneration in bone defects.

scholarly journals Extreme bone lengthening by bone transport with a unifocal tibial corticotomy: a case report

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Hongjie Wen ◽  
Huagang Yang ◽  
Yongqing Xu
Keyword(s):  
Bone Defect ◽  
External Fixator ◽  
Bone Repair ◽  
Bone Defects ◽  
Bone Transport ◽  
External Fixators ◽  
Bone Lengthening ◽  
Fracture Nonunion ◽  
Defect Repair ◽  
The Right

Abstract Background Bone transport is used for the treatment of extensive limb bone defects. The application of ring or unilateral external fixators combined with single or double corticotomy are well documented; however, there are few cases adopting a single corticotomy to repair bone defects > 24 cm. Case presentation The present case study describes an 18-year-old male, who was involved in a traffic accident and was diagnosed with open fracture of the right tibia. The patient received emergency surgery in a local hospital and was transferred to The Second People’s Hospital of Yunnan for further treatment 3 months later. The patient was diagnosed with fracture nonunion and infection following admission. Complete debridement was performed three times to control the infection. The infection was resolved after 26 days and the 24.5 cm massive tibia defect remained the biggest challenge. The bone transport technique involving a unilateral external fixator and single corticotomy was employed to treat the bone defect. Docking site union was achieved and bone consolidation was complete 40 months after corticotomy. The external fixator was subsequently removed. The bone healing index was 1.6 months/cm. The Association for the Study and Application of the Method of Ilizarov criteria (ASAMI) revealed a good functional and bone repair result. Similarly, Knee Society Score (KSS) yielded good result and the The Lower Extremity Functional Scale (LEFS) was 65. A total of 45 months after injury, the patient was able to walk painlessly without ambulatory assistive devices and resumed daily activities successfully. Eighteen months after the bone and soft tissue wound have healed, the SF-36 score was 86, and the LEFS was 70. Conclusion To the best of the authors’ knowledge, the present study described the longest bone defect repair performed using bone transport with single level corticotomy.

scholarly journals In VivoOsteogenesis of Vancomycin Loaded Nanohydroxyapatite/Collagen/Calcium Sulfate Composite for Treating Infectious Bone Defect Induced by Chronic Osteomyelitis

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Xiaojie Lian ◽  
Kezheng Mao ◽  
Xi Liu ◽  
Xiumei Wang ◽  
Fuzhai Cui
Keyword(s):  
Bone Graft ◽  
Bone Defect ◽  
Calcium Sulfate ◽  
Chronic Osteomyelitis ◽  
Rabbit Model ◽  
Calcium Sulphate ◽  
Bone Defects ◽  
Bone Graft Substitute ◽  
Calcium Sulphate Hemihydrate

A novel antibacterial bone graft substitute was developed to repair bone defects and to inhibit related infections simultaneously. This bone composite was prepared by introducing vancomycin (VCM) to nanohydroxyapatite/collagen/calcium sulphate hemihydrate (nHAC/CSH). XRD, SEM, and CCK-8 tests were used to characterize the structure and morphology and to investigate the adhesion and proliferation of murine osteoblastic MC3T3-E1 cell on VCM/nHAC/CSH composite. The effectiveness in restoring infectious bone defects was evaluatedin vivousing a rabbit model of chronic osteomyelitis. Ourin vivoresults implied that the VCM/nHAC/CSH composite performed well both in antibacterial ability and in bone regeneration. This novel bone graft substitute should be very promising for the treatment of bone defect-related infection in orthopedic surgeries.

scholarly journals 3D printed scaffold combined to 2D osteoinductive coatings to repair a critical-size mandibular bone defect

2020 ◽  
Author(s):  
Michael Bouyer ◽  
Charlotte Garot ◽  
Paul Machillot ◽  
Julien Vollaire ◽  
Vincent Fitzpatrick ◽  
...  
Keyword(s):  
Bone Defect ◽  
Bone Repair ◽  
Critical Size ◽  
Bone Defects ◽  
Dose Dependence ◽  
Mandibular Bone ◽  
3D Printed ◽  
Bone Autograft ◽  
Large Bone ◽  
Large Bone Defects

Abstractthe reconstruction of large bone defects (12 cm3) remains a challenge for clinicians. We developed a new critical-size mandibular bone defect model on a mini-pig, close to human clinical issues. We analyzed the bone reconstruction obtained by a 3D printed scaffold made of clinical-grade PLA, coated with a polyelectrolyte film delivering an osteogenic bioactive molecule (BMP-2). We compared the results (CT-scan, μCT, histology) to the gold standard solution, bone autograft. We demonstrated that the dose of BMP-2 delivered from the scaffold significantly influenced the amount of regenerated bone and the repair kinetics, with a clear BMP-2 dose-dependence. Bone was homogeneously formed inside the scaffold without ectopic bone formation. The bone repair was as good as for the bone autograft. The BMP-2 doses applied in our study were reduced 20 to 75-fold compared to the commercial collagen sponges used in the current clinical applications, without any adverse effects. 3D printed PLA scaffolds loaded with reduced doses of BMP-2 can be a safe and simple solution for large bone defects faced in the clinic.

scholarly journals Comparison Study of the Histomorphometric Results after Socket Preservation with PRF and Allograft Used for Socket Preservation—Randomized Controlled Trials

2021 ◽  
Vol 18 (14) ◽  
pp. 7451
Author(s):  
Vasilena Ivanova ◽  
Ivan Chenchev ◽  
Stefan Zlatev ◽  
Eitan Mijiritsky
Keyword(s):  
Connective Tissue ◽  
Bone Formation ◽  
Bone Biopsy ◽  
Bone Allograft ◽  
Point Of View ◽  
Control Group ◽  
Socket Preservation ◽  
Freeze Dried ◽  
Implant Restoration ◽  
Post Hoc

The aim of the present clinical study was to assess and compare the histomorphometric results and efficacy of freeze-dried bone allograft (FDBA) in combination with platelet-rich fibrin (PRF), and PRF as a sole grafting material for socket preservation. Ninety patients in need of tooth extraction and implant restoration were included in this study. The participants were randomly divided into three groups based on post-extraction clinical protocol: socket preservation procedure with allograft in combination with a PRF membrane (PRFm), PRF as a sole grafting material, and a control group. A total of 90 implants were placed four months post-extraction. During the surgical re-entry a bone biopsy was harvested with a trephine drill. Histological samples were prepared and analyzed for percentage vital bone and connective tissue. One-way ANOVA with Bonferroni post-hoc analysis were used to assess the results. Both test groups revealed a significantly higher percentage of vital bone formation compared to the control group. No statistically significant differences regarding vital bone formation and connective tissue quantity between the tested groups were observed (FDBA + PRFm: 3.29 ± 13.03%; and PRF: 60.79 ± 9.72%). From a clinical and histological point of view, both materials in the test groups are suitable for the filling of post-extraction sockets without bone defects. Both of the tested groups revealed a significantly higher percentage of vital bone formation compared to the control group.

scholarly journals Biological behavior of magnesium-substituted hydroxyapatite during bone repair

2020 ◽  
Author(s):  
G. G. Santos ◽  
V. L. C. Nunes ◽  
S. M. O. C. Marinho ◽  
S. R. A. Santos ◽  
A. M. Rossi ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Bone Defect ◽  
Bone Repair ◽  
Bone Mineralization ◽  
Inflammatory Cells ◽  
Biological Behavior ◽  
Osteogenic Potential ◽  
Fibrous Connective Tissue ◽  
Clinical Interest ◽  
Histological Results

Abstract The aim of this study was to analyze the biological behavior and osteogenic potential of magnesium (Mg) substituted hydroxyapatite (HA) microspheres, implanted in a critical bone defect, considering that this ion is of great clinical interest, since it is closely associated with homeostasis and bone mineralization. For the purpose of this study, 30 rats were used to compose three experimental groups: GI - bone defect filled with HA microspheres; GII - bone defect filled with HA microspheres replaced with Mg; GIII - empty bone defect; evaluated at biological points of 15 and 45 days. The histological results, at 15 days, showed, in all the groups, a discrete chronic inflammatory infiltrate; biomaterials intact and surrounded by connective tissue; and bone neoformation restricted to the borders. At 45 days, in the GI and GII groups, an inflammatory response of discrete granulomatous chronic type was observed, and in the GIII there was a scarce presence of mononuclear inflammatory cells; in GI and GII, the microspheres were seen to be either intact or fragmented, surrounded by fibrous connective tissue rich in blood vessels; and discrete bone neoformation near the edges and surrounding some microspheres. In GIII, the mineralization was limited to the borders and the remaining area was filled by fibrous connective tissue. It was concluded that the biomaterials were biocompatible and osteoconductive, and the percentage of Mg used as replacement ion in the HA did not favor a greater bone neoformation in relation to the HA without the metal.

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