Comparison of the accuracy of pilot-drill-guided and fully guided implant surgery with dynamic navigation. In vitro model study

2021 ◽  
Author(s):  
Umut Demetoglu ◽  
Suheyb Bilge ◽  
Senol Aslan ◽  
Hasan Onur Simsek
Keyword(s):  
Navigation System ◽  
Control Group ◽  
Study Group ◽  
Angular Deviation ◽  
Navigation Systems ◽  
Pilot Hole ◽  
Model Study ◽  
Dynamic Navigation ◽  
Vitro Model

The aims of the present study are to measure and compare dental implant deviations with fully guided and pilot-drill-guided protocols using dynamic navigation systems in polyurethane models. The pilot-drill-guided group was determined to be the study group and included 12 implant applications. In this group, the pilot hole was drilled with navigation guidance, and the procedure was completed freehandedly. In the control group, all the drilling and implant placement steps were performed using the navigation system, and a total of 12 implants were placed. The pre- and postoperative images were compared to calculate the magnitude of implant deviation. The quantitative data of the two groups were compared using the independent-samples t-test and Mann-Whitney U-test. The analyses revealed that the length of the procedure significantly differed between the two groups (p < 0.001). The procedure duration was significantly shorter in the study group. The entry deviation values of the two groups were not significantly different (p = 0.079). The analysis revealed the apex deviation to be higher in the study group than in the control group (p = 0.003). However, the two-dimensional vertical apex deviation values of the implants were not significantly different between groups (p = 0.068). Angular deviation was determined to be significantly higher in the study group (p < 0.001). In the present study, all implants were successfully placed in the models using a dynamic navigation system. The results of this study may be useful for future clinical studies.

Navigated versus conventional drilling of the knee in a model of obesity

Open Medicine ◽  
2008 ◽  
Vol 3 (1) ◽  
pp. 89-95
Author(s):  
Johannes Beckmann ◽  
Markus Tingart ◽  
Juergen Goetz ◽  
Sven Anders ◽  
Joachim Grifka ◽  
...  
Keyword(s):  
Navigation System ◽  
Femoral Condyle ◽  
Core Decompression ◽  
Control Group ◽  
Obese Patients ◽  
Significant Difference ◽  
Vitro Model ◽  
Radiation Time ◽  
Conventional Drilling

AbstractCore decompression by exact drilling into ischemic lesions of the femoral condyle stays a challenging procedure, particularly in obese patients. The precision of fluoroscopically-based drilling compared to conventional drilling was evaluated in an in-vitro model for obesity. 40 sawbones were prepared mimicking osteonecrosis in obese patients. 20 sawbones were drilled by guidance of an intraoperative navigation system VectorVision® (BrainLAB, Munich, Germany); the other 20 sawbones were conventionally drilled by fluoroscopic control only. Regarding the distance to the desired mid-point of the lesion, a statistically significant difference with 0.55 mm in the navigated group and 1.19 mm in the control group could be stated (p<0.001). Significant differences were further found for the number of drilling corrections (p<0.001) and radiation time needed (p<0.001). Even in difficult circumstances as in obese patients, the drilling guided by the VectorVision®-navigation system shows high precision with simultaneous enormous reduction of radiation time.

scholarly journals MicroRNA-1297 suppressed the Akt/GSK3β signaling pathway and stimulated neural apoptosis in an in vivo sevoflurane exposure model

2021 ◽  
Vol 49 (4) ◽  
pp. 030006052098210
Author(s):  
Quan Wang ◽  
Jingcong Luo ◽  
Ruiqiang Sun ◽  
Jia Liu
Keyword(s):  
Protein Expression ◽  
Signaling Pathway ◽  
Neuronal Apoptosis ◽  
In Vitro Model ◽  
Nervous System Development ◽  
Exposure Model ◽  
Control Group ◽  
Pten Protein ◽  
Vitro Model

Objective Common inhalation anesthetics used for clinical anesthesia (such as sevoflurane) may induce nerve cell apoptosis during central nervous system development. Furthermore, anesthetics can produce cognitive impairments, such as learning and memory impairments, that continue into adulthood. However, the precise mechanism remains largely undefined. We aimed to determine the function of microRNA-1297 (miR-1297) in sevoflurane-induced neurotoxicity. Methods Reverse transcription-polymerase chain reaction assays were used to analyze miR-1297 expression in sevoflurane-exposed mice. MTT and lactate dehydrogenase (LDH) assays were used to measure cell growth, and neuronal apoptosis was analyzed using flow cytometry. Western blot analyses were used to measure PTEN, PI3K, Akt, and GSK3β protein expression. Results In sevoflurane-exposed mice, miR-1297 expression was up-regulated compared with the control group. MiR-1297 up-regulation led to neuronal apoptosis, inhibition of cell proliferation, and increased LDH activity in the in vitro model of sevoflurane exposure. MiR-1297 up-regulation also suppressed the Akt/GSK3β signaling pathway and induced PTEN protein expression in the in vitro model. PTEN inhibition (VO-Ohpic trihydrate) reduced PTEN protein expression and decreased the effects of miR-1297 down-regulation on neuronal apoptosis in the in vitro model. Conclusion Collectively, the results indicated that miR-1297 stimulates sevoflurane-induced neurotoxicity via the Akt/GSK3β signaling pathway by regulating PTEN expression.

Influence of biofilm removal from the tooth-restoration interface on the progression of secondary caries lesions: a preliminary in vitro model study

Biofouling ◽  
2021 ◽  
pp. 1-12
Author(s):  
Cácia Signori ◽  
Tamires Timm Maske ◽  
Vitor Henrique Digmayer Romero ◽  
Maximiliano Sérgio Cenci
Keyword(s):  
In Vitro Model ◽  
Secondary Caries ◽  
Model Study ◽  
Biofilm Removal ◽  
Vitro Model ◽  
Caries Lesions

Effect of fluoride-releasing restorative materials on bacteria-induced pH fall at the bacteria–material interface: An in vitro model study

2014 ◽  
Vol 42 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Gen Mayanagi ◽  
Koei Igarashi ◽  
Jumpei Washio ◽  
Hitomi Domon-Tawaraya ◽  
Nobuhiro Takahashi
Keyword(s):  
In Vitro Model ◽  
Material Interface ◽  
Model Study ◽  
Restorative Materials ◽  
Vitro Model

Potential of Raloxifene in reversing osteoarthritis-like alterations in rat chondrocytes: An in vitro model study

2012 ◽  
Vol 38 (1) ◽  
pp. 135-147 ◽  
Author(s):  
Aysegul Kavas ◽  
Seda Tuncay Cagatay ◽  
Sreeparna Banerjee ◽  
Dilek Keskin ◽  
Aysen Tezcaner
Keyword(s):  
In Vitro Model ◽  
Model Study ◽  
Vitro Model

Studies in Cranial Suture Biology: In Vitro Cranial Suture Fusion

1996 ◽  
Vol 33 (2) ◽  
pp. 150-156 ◽  
Author(s):  
James P. Bradley ◽  
Jamie P. Levine ◽  
Christopher Blewett ◽  
Thomas Krummel ◽  
Joseph G. Mccarthy ◽  
...  
Keyword(s):  
Organ Culture ◽  
In Vitro Model ◽  
In Vitro Study ◽  
Cranial Base ◽  
Cranial Suture ◽  
Model Study ◽  
Suture Fusion ◽  
Vitro Model

The biology underlying craniosynostosis remains unknown. Previous studies have shown that the underlying dura mater, not the suture itself, signals a suture to fuse. The purpose of this study was to develop an in vitro model for cranial-suture fusion that would still allow for suture-dura interaction, but without the influence of tensional forces transmitted from the cranial base. This was accomplished by demonstrating that the posterior frontal mouse cranial suture, known to be the only cranial suture that fuses in vivo, fuses when plated with its dura in an organ-culture system. In such an organ-culture system, the sutures are free from both the influence of dural forces transmitted from the cranial base and from hormonal influences only available in a perfused system. For the cranial-suture fusion in vitro model study, the sagittal sutures (controls that remain patent in vivo) and posterior frontal sutures (that fuse in vivo) with the underlying dura were excised from 24-day-old euthanized mice, cut into 5 × 4 × 2-mm specimens, and cultured in a chemically defined, serum-free media. One hundred sutures were harvested at the day of sacrifice, then every 2 days thereafter until 30 days in culture, stained with H & E, and analyzed. A subsequent cranial-suture without dura in vitro study was performed in a similar fashion to the first study, but only the calvariae with the posterior frontal or sagittal sutures (without the underlying dura) were cultured. Results from the cranial-suture fusion in vitro model study showed that all sagittal sutures placed in organ culture with the underlying dura remained patent. More importantly, the posterior frontal sutures with the underlying dura, which were plated-down as patent at 24 days of age, demonstrated fusion after various growth periods in organ culture. In vitro posterior frontal mouse-suture fusion occurred in an anterior-to-posterior direction but in a delayed fashion, 4 to 7 days later than in vivo posterior frontal mouse-suture fusion. In contrast, the subsequent cranial-suture without dura in vitro study showed patency of all sutures, including the posterior frontal suture. These data from in vitro experiments indicate that: (1) mouse calvariae, sutures, and the underlying dura survive and grow in organ-culture systems for 30 days; (2) the local dura, free from external influences transmitted from the cranial base and hormones from distant sites, influences the cells of its overlying suture to cause fusion; and (3) without dura influence, all in vitro cranial sutures remained patent. By first identifying the factors involved in dural-suture signaling and then regulating these factors and their receptors, the biologic basis of suture fusion and craniosynostosis may be unraveled and used in the future to manipulate pathologic (premature) suture fusion.

scholarly journals Cytotoxic and genotoxic potential of Bulgarian Rosa alba L. essential oil – in vitro model study

2018 ◽  
Vol 32 (2) ◽  
pp. 513-519 ◽  
Author(s):  
Gabrielle Jovtchev ◽  
Alexander Stankov ◽  
Almira Georgieva ◽  
Anna Dobreva ◽  
Rumiana Bakalova ◽  
...  
Keyword(s):  
Essential Oil ◽  
In Vitro Model ◽  
Genotoxic Potential ◽  
Model Study ◽  
Vitro Model

scholarly journals Anti-Proliferative Effects of HBX-5 on Progression of Benign Prostatic Hyperplasia

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2638 ◽  
Author(s):  
Bo-Ram Jin ◽  
Hyo-Jung Kim ◽  
Sang-Kyun Park ◽  
Myoung-Seok Kim ◽  
Kwang-Ho Lee ◽  
...  
Keyword(s):  
Benign Prostatic Hyperplasia ◽  
Prostatic Hyperplasia ◽  
Nuclear Antigen ◽  
Control Group ◽  
Over Expression ◽  
Alternative Medicines ◽  
Androgenic Hormone ◽  
Vitro Model ◽  
Proliferating Cell

Benign prostatic hyperplasia (BPH), an age-dependent disorder with a prevalence percentage of 60% in the 60s, has been found to involve an androgenic hormone imbalance that causes confusion between cell apoptosis and proliferation. Because general medications for BPH treatment have undesirable side effects, the development of effective alternative medicines has been considered. HBX-5 is a newly developed formula with the aim of improving BPH, and is composed of nine medicinal herbs. BPH was induced in the rats by intramuscular injection of testosterone propionate after castration. Rats were divided into six groups, and the efficacy of HBX-5 on testosterone-induced BPH in rats was estimated. In addition, RWPE-1 and WPMY-1 cells were used to demonstrate the effect of HBX-5 on BPH in vitro model. Compared with the control group, HBX-5 administration group suppressed BPH manifestations, such as excessive development of prostate, and increase of serum dihydrotestosterone and 5α-reductase concentrations. Furthermore, immunohistochemistry analysis revealed that HBX-5 significantly decreased the expression of androgen receptor (AR) and proliferating cell nuclear antigen (PCNA). In addition, results of RWPE-1 and WPMY-1 cells showed that HBX-5 inhibited the over-expression of AR and PSA in DHT-induced prostate hyperplastic microenvironments.

scholarly journals Accelerometer-Based Navigation in Total Knee Arthroplasty for the Management of Extra-Articular Deformity and Retained Femoral Hardware: Analysis of Component Alignment

Joints ◽  
2019 ◽  
Vol 07 (01) ◽  
pp. 001-007 ◽  
Author(s):  
Andrea Cozzi Lepri ◽  
Matteo Innocenti ◽  
Fabrizio Matassi ◽  
Marco Villano ◽  
Roberto Civinini ◽  
...  
Keyword(s):  
Total Knee Arthroplasty ◽  
Knee Arthroplasty ◽  
Navigation System ◽  
Lower Limb ◽  
Sagittal Plane ◽  
Frontal Plane ◽  
Control Group ◽  
Navigation Systems ◽  
Level Of Evidence ◽  
Total Knee

Abstract Purpose Recent advances in total knee arthroplasty (TKA) include an accelerometer portable system designed to improve component position and alignment. The purpose of this study is to evaluate whether accelerometer navigation system can be a valuable option in complex TKAs for extra-articular deformity of the lower limb or in case of retained femoral hardware. Methods A group of 13 patients underwent TKA with an accelerometer navigation system. Three patients had a tibial extra-articular deformity, six had a femoral extra-articular deformity, and four had an intramedullary nail in the femur. Preoperative and postoperative mechanical axes were measured from full-length lower extremity radiographs to evaluate alignment. The alignment of prosthetic components in the frontal and sagittal planes was determined by postoperative radiographs. Results At 30-days postoperative radiographic check, the hip knee ankle angle was within 2.0° (0 ± 1) of the neutral mechanical axis. The alignment of the tibial component on the frontal plane was 90.0° (range 89–91) and on the sagittal plane 5.0° (range 3–7). The alignment of the femoral component on the frontal plane was 90.0° (range 89–91) and on the sagittal plane 3.0° (range 0–5). Conclusion The alignment of the prosthetic components has been accurate and comparable to other navigation systems in literature without any increase in surgical times. The accelerometer-based navigation system is therefore a useful technique that can be used to optimize TKA alignment in patients with extra-articular deformity or with lower limb hardware, where the intramedullary guides cannot be applied. Level of Evidence This is an observational study without a control group, Level III.

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