CHANGES IN VASCULAR ENDOTHELIAL GROWTH FACTOR LEVEL IN HELICOBACTER PYLORI-ASSOCIATED GASTRODUODENAL DISEASES

A populational infection with Helicobacter (H.) pylori poses a global problem. Mucosal colonization of H. pylori in the gastroduodenal area can initiate development multiple diseases with hyper-or hypoplasia of mucosal epithelial cells secreting vascular endothelial growth factor (VEGF). The aim of the study was to assess VEGF serum level, its diagnostic and prognostic value in diseases affecting the gastroduodenal area. Material and methods. 180 patients with exacerbated chronic gastritis, gastric ulcer, duodenal ulcer as well as 30 healthy volunteers were examined after providing an informed consent. Patients were divided into groups depending on the degree of mucous contamination with H. pylori. In the subjects examined during esophagogastroduodenoscopy, a biological material was collected during targeted biopsy for microscopic and histological studies. Blood samples for immunological examination were obtained in the morning on an empty stomach from the ulnar vein in the volume of 5 ml, and the serum was isolated by centrifugation. The level of VEGF, pepsinogens, and titer of total antibodies against the H. pylori cytotoxin-associated protein were determined in the blood serum from the subjects by using the enzyme immunoassay method. The long-term prognosis was analyzed for up to 2 years. The data obtained were processed statistically. Results. Patients were found to have excessive serum VEGF levels in healthy volunteers. For gastric ulcer associated with H. pylori, 80% of cases had increased discriminatory VEGF level. In patients, direct relationships between the serum VEGF level and degree, stage of gastritis, the degree of contamination with H. pylori, the serum pepsinogens level were uncovered. Regression analysis found that patients with diseases targeting gastroduodenal area had serum VEGF level equal to or greater than 231 pg/ml in 60% of cases that correctly predicted an increase in mucosal atrophy. If the amount of VEGF ≥373 pg/ml in 91.5% of cases, then ulceration of gastric epithelium developed, whereas for ≥396 pg/ml level it was observed in 89% cases with ulceration of the intestinal epithelium. The probability of gastroduodenal bleeding at a serum VEGF level of 408 pg/ml or higher was predicted correctly in 96% of cases. Conclusion. More than 54% of patients with H. pylori-associated chronic gastritis, peptic ulcer disease had level of VEGF significantly exceeding magnitude found in healthy volunteers and the discriminatory level reflects the morphofunctional state of the stomach and duodenum. Assessing serum VEGF level in gastroduodenal diseases can be recommended for predicting development of atrophy, ulceration of the gastric and intestinal epithelium, and gastroduodenal bleeding.

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VASCULAR ENDOTHELIAL GROWTH FACTOR IN CHILDREN WITH THALASSEMIA MAJOR PDF

Mediterranean Journal of Hematology and Infectious Diseases ◽

10.4084/mjhid.2013.044 ◽

2013 ◽

Vol 5 (1) ◽

pp. e2013044 ◽

Cited By ~ 7

Author(s):

Sameh Samir Fahmey ◽

Hassan Naguib ◽

Sanna Abdelshafy ◽

Rasha Alashry

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Blood Transfusion ◽

Elevated Serum ◽

Thalassemia Major ◽

Enzyme Linked Immunosorbent Assay ◽

Vascular Endothelial ◽

Healthy Controls ◽

Endothelial Growth Factor ◽

Vegf Level

Background: The β-Thalassemia syndromes are the most common hereditary chronic hemolytic anemia due to impaired globin chain synthesis. Vascular endothelial growth factor (VEGF) plays several roles in angiogenesis which is a crucial process in the pathogenesis of several inflammatory, autoimmune and malignant diseases .Endothelial damage and inflammation make a significant contribution to the pathophysiology of β-thalassemia. Purpose: The aim of the study was to assess serum VEGF level in children with beta-thalassemia major as a marker of angiogenesis. Methods: Blood samples were collected from 40 patients with thalassemia major and 10 healthy controls and assayed for VEGF by enzyme-linked immunosorbent assay. Results: VEGF level was significantly higher in patients with β-Thalassemia major than healthy controls (p=0.001).In addition, VEGF level was higher in splenectomised thalassemic patients than non splenectomised ones (p=0.001) .However, there were a positive correlation between VEGF and chelation starting age (p=0.008) and a negative correlation between VEGF and frequency of blood transfusion (p=0.002). Conclusion: thalassemia patients, especially splenectomized, have elevated serum levels of VEGF. Early chelation and regular blood transfusion help to decrease serum VEGF and the risk of angiogenesis.

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Vascular endothelial growth factor is of high prognostic value in node-negative breast carcinoma.

Journal of Clinical Oncology ◽

10.1200/jco.1998.16.9.3121 ◽

1998 ◽

Vol 16 (9) ◽

pp. 3121-3128 ◽

Cited By ~ 183

Author(s):

B Linderholm ◽

B Tavelin ◽

K Grankvist ◽

R Henriksson

Keyword(s):

Vascular Endothelial Growth Factor ◽

Overall Survival ◽

Growth Factor ◽

Breast Carcinoma ◽

Systemic Therapy ◽

Prognostic Value ◽

Vascular Endothelial ◽

Node Negative ◽

Endothelial Growth Factor ◽

Vegf Level

PURPOSE The prognostic value of vascular endothelial growth factor (VEGF) protein, known to stimulate endothelial growth and angiogenesis, was evaluated in node-negative breast carcinoma (NNBC) and compared with established prognostic factors. PATIENTS AND METHODS In 525 consecutive patients with primary invasive NNBC (T1-2N0M0; tumor, node, metastasis stage), of whom 500 patients did not receive any systemic therapy, the cytosolic levels of VEGF165 were measured by using a quantitative enzyme-linked immunosorbent assay. The median follow-up was 46 months. Univariate and multivariate analyses were performed. RESULTS VEGF level was significantly inversely correlated with estrogen receptor (ER) positivity but positively associated with tumor size and histologic grade. Patients with VEGF levels above the median value (2.40 pg/microg of DNA) showed a significantly shorter survival time (P=.0012) than patients with levels less than the median value, also when analyzed as a continuous variable (P=.0277). Tumor size, grade, and ER expression were all statistically significant for overall survival in univariate analyses (P=.0069, P=.014, and P < .001, respectively). Multivariate analysis showed that VEGF level was the strongest predictor of overall survival (P=.0199). Histologic grade was also an independent predictor of survival (P=.0477). Among the 381 patients with ER-positive tumors, a group in general considered to have a good prognosis, we found a significant reduction in survival for those with levels of VEGF greater than the median value (P=.0009). CONCLUSION The results suggest that the level of VEGF165 protein is an independent, strong prognostic factor for survival in patients with NNBC, especially in the subgroup of patients with ER positivity. Thus, cytosolic VEGF165 might be useful to select patients for adjuvant systemic therapy.

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Effect Of Coal Dust Exposure To Serum Interleukin 6 (IL6) And Vascular Endothelial Growth Factor (VEGF) Level As Preeclampsia Induction In Pregnant Rattus Novergicus

Journal of Physics Conference Series ◽

10.1088/1742-6596/1374/1/012056 ◽

2019 ◽

Vol 1374 ◽

pp. 012056

Author(s):

S Wasilah ◽

F Rachman ◽

D Rosadi ◽

A O Puteri ◽

N Laily ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Interleukin 6 ◽

Coal Dust ◽

Dust Exposure ◽

Vascular Endothelial ◽

Endothelial Growth Factor ◽

Vegf Level

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Estrogen-Dependent Growth of a Rat Pituitary Tumor Involves, But Does Not Require, a High Level of Vascular Endothelial Growth Factor

Experimental Biology and Medicine ◽

10.1177/153537020222700714 ◽

2002 ◽

Vol 227 (7) ◽

pp. 492-499 ◽

Cited By ~ 7

Author(s):

Danny Cracchiolo ◽

Jason W. Swick ◽

Lucy McKiernan ◽

Erica Sloan ◽

Supriya Raina ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Pituitary Tumor ◽

Vascular Endothelial ◽

Vegf Expression ◽

Rat Pituitary ◽

High Level ◽

Endothelial Growth Factor ◽

F344 Rats ◽

Vegf Level

Long-term (10-week) treatment of Fischer 344 (F344) rats with the synthetic estrogen diethylstilbestrol (DES) increases the level of vascular endothelial growth factor (VEGF) in the pituitary. This is concurrent with the development of a large tumor of the pituitary of F344 rats. A role for VEGF in estrogendependent pituitary tumor growth is also supported by the fact that pituitary VEGF level is not increased by estrogen treatment in rats of the tumor-resistant Brown Norway (BN) strain. However, VEGF is not increased by estrogen treatment in an F1 hybrid of F344 and BN, even though F1 hybrid rats do form pituitary tumors in response to estrogen. Quantitative trait locus (QLT) mapping reveals that control of estrogen-dependent VEGF expression is linked to the Edpm5 QTL, which was previously identified as a QTL for estrogen-dependent pituitary tumor growth. In contrast, the QTL Edpm2-1 and Edpm9-2, which have been shown to each have a significant effect on estrogendependent pituitary mass of a magnitude similar to Edpm5, do not have any effect on VEGF level. Taken together, our results support the association of VEGF expression with growth of the estrogen-Induced rat pituitary tumor, as has been reported by others, but they also indicate that there is significant pathways of growth regulation that are independent of high-level VEGF expression.

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Cellular Vascular Endothelial Growth Factor Is a Predictor of Outcome in Patients With Acute Myeloid Leukemia

Blood ◽

10.1182/blood.v94.11.3717 ◽

1999 ◽

Vol 94 (11) ◽

pp. 3717-3721 ◽

Cited By ~ 267

Author(s):

Alvaro Aguayo ◽

Elihu Estey ◽

Hagop Kantarjian ◽

Taghi Mansouri ◽

Cristi Gidel ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Acute Myeloid Leukemia ◽

Growth Factor ◽

Myeloid Leukemia ◽

Prognostic Significance ◽

Vascular Endothelial ◽

Newly Diagnosed ◽

Endothelial Growth Factor ◽

Vegf Level ◽

Acute Myeloid

Abstract Vascular endothelial growth factor (VEGF) is a potent mitogen for vascular endothelial cells. It has been associated with angiogenesis, growth, dissemination, metastasis, and poor outcome in solid tumors. To assess cellular VEGF levels and their prognostic significance in newly diagnosed acute myeloid leukemia (AML), we used a radioimmunoassay (RIA) to quantify VEGF levels in stored samples obtained before treatment from 99 patients with newly diagnosed AML treated at the MD Anderson Cancer Center from 1996 to 1998. Outcome in the 99 patients was representative of that observed in all patients seen at this institution with this diagnosis during these years, but the 99 patients had higher white blood cell (WBC) and blast counts than the other patients. Results of the RIA were confirmed by Western blot. There was a relationship between increasing VEGF levels and shorter survival (P = .01), as well as shorter disease-free survival, both from start of treatment and from complete response (CR) date. In contrast, there was no relationship between VEGF level and WBC or blast count, or between VEGF level and such established prognostic factors as age, cytogenetics, performance status, or presence of an antecedent hematologic disorder, and multivariate analysis indicated that VEGF was still prognostic for the above outcomes after accounting for these factors, as well as treatment. Our results suggest that at least in AML patients with higher WBC and blast counts, cellular VEGF level is an independent predictor of outcome.

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Expression of Vascular Endothelial Growth Factor at the Human Gastric Ulcer Margin and in Cultured Gastric Fibroblasts: A New Angiogenic Factor for Gastric Ulcer Healing

Biochemical and Biophysical Research Communications ◽

10.1006/bbrc.1997.5974 ◽

1997 ◽

Vol 234 (2) ◽

pp. 493-498 ◽

Cited By ~ 46

Author(s):

Morio Takahashi ◽

Takao Kawabe ◽

Keiji Ogura ◽

Shin Maeda ◽

Yasuyo Mikami ◽

...

Keyword(s):

Vascular Endothelial Growth Factor ◽

Gastric Ulcer ◽

Growth Factor ◽

Ulcer Healing ◽

Angiogenic Factor ◽

Vascular Endothelial ◽

Gastric Ulcer Healing ◽

Ulcer Margin ◽

Endothelial Growth Factor

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Clinical Significance of Serum Levels of Vascular Endothelial Growth Factor, Angiopoietin-1, and Angiopoietin-2 in Patients with Rheumatoid Arthritis

The Journal of Rheumatology ◽

10.3899/jrheum.090941 ◽

2010 ◽

Vol 37 (6) ◽

pp. 1121-1128 ◽

Cited By ~ 44

Author(s):

DAITARO KUROSAKA ◽

KENICHIRO HIRAI ◽

MAKIKO NISHIOKA ◽

YUKIO MIYAMOTO ◽

KEN YOSHIDA ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Vascular Endothelial Growth Factor ◽

Growth Factor ◽

Serum Levels ◽

Vascular Endothelial ◽

Angiopoietin 2 ◽

Serum Crp ◽

Endothelial Growth Factor ◽

Vegf Level ◽

Angiopoietin 1

Objective.To evaluate the clinical significance of serum levels of vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1), and angiopoietin-2 (Ang-2) in patients with rheumatoid arthritis (RA).Methods.The subjects were 70 patients with RA. Serum VEGF, Ang-1, and Ang-2 levels were determined by ELISA. As indices of disease activity, serum levels of C-reactive protein (CRP) and matrix metalloprotease (MMP)-3 were examined, and the 28-joint count Disease Activity Score (DAS28)-CRP was calculated. Power Doppler ultrasonography was performed in the bilateral wrists, elbows, shoulders, knees and ankles. The synovial blood flow signals were scored using a 3-grade scale (0–2), and the total of the scores in the 10 joints was regarded as the total signal score (TSS).Results.Serum VEGF level showed significant correlations with serum CRP and MMP-3 levels, DAS28-CRP, and TSS. Serum Ang-1 level showed significant correlations with serum MMP-3 level and DAS28-CRP. Serum Ang-2 level showed significant correlations with serum CRP level and TSS.Conclusion.The serum VEGF level is important as an index of the activity of RA based on angiogenesis and a prognostic factor regarding joint destruction. Serum Ang-1 level may be useful as an index of sustained arthritis based on the maintenance of newly formed vessels. Serum Ang-2 level may reflect a state of marked angiogenesis.

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