scholarly journals Geographical diversity of Helicobacter pylori strains circulating in the European Part of the Russian Federation

2021 ◽  
Vol 11 (4) ◽  
pp. 701-706
Author(s):  
V. M. Sorokin ◽  
A. V. Svarval ◽  
A. S. Vodop'janov ◽  
R. V. Pisanov
Keyword(s):  
Russian Federation ◽  
Geographical Origin ◽  
Diversity Index ◽  
European Part ◽  
Typing Method ◽  
Population Total ◽  
Saint Petersburg ◽  
Indel Markers ◽  
The Russian Federation ◽  
H Pylori

The aim of the study was to identify INDEL markers and study geographical origin of regional H. pylori strains circulating in the European part of the Russian Federation. The study included 56 strains of H. pylori isolated in three regions of the Russian Federation: Saint Petersburg, Astrakhan and Rostov Regions. Genomic DNA was isolated using a set of Probe NA (DNA Technology, Russia), according to the manufacturer’s instructions. Detection of INDEL markers hp5605, hp6405, hp340, hp1390, hp3660 was performed using PCR. Clustering of the identified INDEL genotypes and building a phylogenetic tree were performed using the BioNumerics 7.6 and GrapeTree software packages. 21 strains from the GenBank database with known geographical origin were used as reference strains. In 20 strains from Saint Petersburg, 13 individual genotypes were identified, while 17 strains belong to the European cluster (hpEurope), 2 strains belong to the hspEAsia cluster and one strain belongs to the hspWAfrica cluster. The most common genotype identified in the European cluster includes six strains from Saint Petersburg and two strains from the GenBank database. For further differentiation of these strains, the VNTR typing method was used, which allowed identifying eight individual genotypes in eight strains. Fifty-six studied russian strains are represented by thirty individual genotypes, which reflects the high heterogeneity of strains circulating in the European part of the Russian Federation. The most frequent genotype is represented by two hpEurope strains, one strain from the Astrakhan region, as well as 5 and 6 strains from the Rostov Region and Saint Petersburg, respectively. The vast majority of Russian strains (52/56) belong to the hpEurope population, while two strains from Saint Petersburg are included in the hspEAsia population, and one strain from Saint Petersburg and the Astrakhan Region is included in the hspWAfrica population. Total, 77 H. pylori strains are represented by 37 individual genotypes with a high diversity index (DI = 0.95), which allows us to consider the proposed INDEL typing method as an independent method for genotyping H. pylori strains. Taking into consideration the complexity of the problem of accurately determining the geographical origin of H. pylori strains, the proposed simple and convenient method of INDEL typing of H. pylori strains, based on an available PCR method becomes very relevant and allows us to conduct an adequate primary analysis of the geographical origin of Russian H. pylori strains.

INDEL-typing: a new method of differentiation of Helicobacter pylori strains

Bacteriology ◽  
2020 ◽  
Vol 5 (1) ◽  
pp. 8-13
Author(s):  
V.M. Sorokin ◽  
◽  
A.S. Vodop’janov ◽  
R.V. Pisanov ◽  
◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Geographical Origin ◽  
Diversity Index ◽  
Minimal Spanning Tree ◽  
Typing Method ◽  
Indel Markers ◽  
H Pylori ◽  
First Time ◽  
Tree Method ◽  
High Diversity

For the first time INDEL-typing method of Helicobacter pylori strains is offered. Analysis of the nucleotide sequences of 69 strains from the local database was carried out. Ten INDEL-markers were identified, five of which, with the highest variability, served as the basis for the differentiation scheme. INDEL-typing of twenty one strains of H. pylori of different geographical origin was carried out. Аnd fifteen individual genotypes with a high diversity index were identified (DI = 0.95). For cluster analysis, the MST (minimal spanning tree) method was used, which demonstrated a clear distribution of clusters according to the geographical origin of the strains studied. In addition to the proposed method of typing, if necessary, the MLVAtyping method can be used. Keywords: Helicobacter pylori, PCR, INDEL, MST dendrogram, MLVA, geographical origin

scholarly journals Antibiotic resistance of Helicobacter pylori in the European part of the Russian Federation: first results

2020 ◽  
Vol 92 (8) ◽  
pp. 24-28
Author(s):  
I. V. Maev ◽  
D. N. Andreev ◽  
V. M. Govorun ◽  
E. N. Ilina ◽  
Yu. A. Kucheryavyy ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Russian Federation ◽  
European Part ◽  
Diffusion Method ◽  
Rapid Urease Test ◽  
Urease Test ◽  
First Results ◽  
The Russian Federation ◽  
H Pylori

Aim. Determine the primary antibiotic resistance of Helicobacter pylori (H. pylori) strains isolated from patients living in the European part of the Russian Federation. Materials and methods. As part of a clinical laboratory study, from 2015 to 2018, 27 gastrobiopsy samples obtained from H. pylori-infected patients were analyzed. H. pylori infection was verified using a rapid urease test or a 13C-urea breath test. The values of the minimum inhibitory concentration (MIC) of antibiotics were determined by the diffusion method using E-test strips (BioMerieux, France) according to the recommendations of the manufacturer. The sensitivity of the isolates was determined for 6 antibacterial drugs (amoxicillin, clarithromycin, metronidazole, levofloxacin, tetracycline, rifampicin). Results. According to the data obtained, resistance to amoxicillin was 0%, clarithromycin 11.1%, metronidazole 59.3%, levofloxacin 3.7%, tetracycline 0%, and rifampicin 14.8%. Dual resistance to clarithromycin and metronidazole was recorded in two isolates (7.4%). Conclusion. Thus, the first results of the evaluation of H. pylori antibiotic resistance in the European part of the Russian Federation indicate a low resistance of the microorganism to clarithromycin and quite high to metronidazole.

scholarly journals Epizootiological Monitoring of Natural Focal Infections in the South of the European Part of the Russian Federation in 2017

2019 ◽  
pp. 45-49
Author(s):  
N. F. Vasilenko ◽  
O. V. Maletskaya ◽  
D. A. Prislegina ◽  
E. A. Manin ◽  
O. V. Semenko ◽  
...  
Keyword(s):  
Russian Federation ◽  
Q Fever ◽  
Hemorrhagic Fever ◽  
European Part ◽  
The South ◽  
Hemorrhagic Fever Virus ◽  
Granulocytic Anaplasmosis ◽  
Control Research ◽  
The Russian Federation ◽  
Human Monocytic Ehrlichiosis

Objective– analysis of epizootiological manifestations of natural focal infections in the territory of the south of the European part of the Russian Federation in 2017.Materials and methods. Statistical documentation data from the Rospotrebnadzor Administrations, Centers of Hygiene and Epidemiology in the constituent entities of the Russian Federation, and Plague Control Research Institutes and Stations were used. The information was processed using Microsoft Excel 2010 software.Results and discussion. Epizootiological survey for 19 nosological forms of natural focal infections in the territory of the south of the European part of the Russian Federation was conducted. The total of 70155 samples of field material was tested; markers of 14 pathogens of natural focal infections were identified. The circulation of Crimean-Congo hemorrhagic fever virus was revealed in 11 constituent entities, tularemia and Lyme borreliosis pathogens – in 8 entities, West Nile virus – in 7. Markers of leptospirosis, Q fever, human granulocytic anaplasmosis and human monocytic ehrlichiosis pathogens were detected in 6 constituent entities, markers of the agent of hemorrhagic fever with renal syndrome – in 5 entities; markers of intestinal yersiniosis pathogen – in 3 constituent entities of the Russian Federation, pathogens of tick spotted fevers group, tick-borne viral encephalitis and pseudotuberculosis – in 2. The circulation of the virus Sindbis was identified in the Rostov Region. 

scholarly journals Human babesiosis: clinical cases in the european part of the Russian Federation

2019 ◽  
Vol 91 (11) ◽  
pp. 60-65
Author(s):  
A K Tokmalaev ◽  
V B Chentsov ◽  
V A Malov ◽  
V V Maleyev ◽  
G M Kozhevnikova ◽  
...  
Keyword(s):  
Differential Diagnosis ◽  
Russian Federation ◽  
Clinical Picture ◽  
Epidemiological Data ◽  
European Part ◽  
Laboratory Studies ◽  
Direct Supervision ◽  
Clinical Observations ◽  
The Russian Federation ◽  
Human Babesiosis

The article describes four clinical observations of patients with babesiosis detected in the European part of the Russian Federation, two of whom were under the direct supervision of the authors. The analysis of epidemiological data, clinical picture, results of laboratory studies in the dynamics of the disease. Differential diagnosis and treatment are discussed.

scholarly journals Stem Volume Tables by Height Classes of Willow Trees in the Northern Taiga Region of the European Part of the Russian Federation

2021 ◽  
pp. 69-78
Author(s):  
Andrey A. Paramonov ◽  
◽  
Sergey V. Tretyakov ◽  
Sergey V. Koptev ◽  
Aleksandr P. Bogdanov ◽  
...  
Keyword(s):  
Russian Federation ◽  
Forest Inventory ◽  
Information Source ◽  
Northern Taiga ◽  
European Part ◽  
Stem Volume ◽  
Analytical Technique ◽  
Height Class ◽  
The Russian Federation ◽  
Willow Trees

In order to improve the accuracy of forest inventory works, tables of stem volume by height classes of willow trees in the northern taiga region of the European part of the Russian Federation were created. Forest inventory standards have not previously been developed for this species in the northern taiga region. The analytical technique proposed by I.I. Gusev in 1971 for spruce forests of the European North was used in compiling the height class volume tables. It was later adopted for the description of other tree species as well. Diameters and heights of trees were measured at 41 sample plots laid out in the Verkhnyaya Toyma and Arkhangelsk forestries of the Arkhangelsk region; 105 model trees were sampled. In most cases, sample plots were laid out in mixed plantations with the predominance of willow. The data obtained on the sample plots served to determine the parameters of the height class scale. The information source for stem volume of willow trees used to be the stem volume tables for aspen trees. A comparative analysis of the tables compiled for willow trees stems with the tables for aspen taken from the Forest Valuation Handbook for the Northeast of the European part of the Russian Federation was carried out. The analysis shows significant discrepancy in the scales for willow and aspen. The use of aspen height class volume tables for willow leads to a systematic overestimation of wood volumes. Tables of stem volumes by height classes of willow trees are used to determine stand stock with the enumeration data by 4-centimeter diameter classes. The developed tables meet the forest practice requirements. The volume tables by height classes are used to calculate timber reserves when laying out sample plots; to determine the damage level in case of stand damage; and for other practical purposes. For citation: Paramonov A.A., Tretyakov S.V., Koptev S.V., Bogdanov A.P., Tsvetkov I.V. Stem Volume Tables by Height Classes of Willow Trees in the Northern Taiga Region of the European Part of the Russian Federation. Lesnoy Zhurnal [Russian Forestry Journal], 2021, no. 6, pp. 69–78. DOI: 10.37482/0536-1036-2021-6-69-78

scholarly journals Tactical approaches to the treatment of arterial hypertension in the European part of the Russian Federation. EPOHA study 2017

Kardiologiia ◽  
2019 ◽  
Vol 59 (11S) ◽  
pp. 77-84
Author(s):  
Yu. V. Badin
Keyword(s):  
Arterial Hypertension ◽  
Antihypertensive Therapy ◽  
Russian Federation ◽  
Antihypertensive Treatment ◽  
Antihypertensive Drugs ◽  
European Part ◽  
Frequency Of Use ◽  
Two Component ◽  
The Russian Federation ◽  
Real Clinical Practice

Aim. Analysis of antihypertensive treatment among different groups of patients with arterial hypertension from “EPOCHA” study 2017. Materials and methods. EPOCHA study is a representative sample of the European part of the Russian Federation was created in 2002 and re-examined in 2017. Results. 34.6% of the effectively treated (ET) patients had one antihypertensive drug and 32,2% of the ineffective treatment (IT) patients, p=0.16. Two-component antihypertensive therapy was 45.9% and 44.9%, respectively, p=0.56. Three antihypertensive drugs had 17.9% of the ET patients and 20.7% of the IT patients, p=0.05. Four antihypertensive drugs had 1.6% of the ET patients and 2.2% of the IT patients. The frequency of use of renin-angiotensin-aldosterone system blockers in the group of ET patients was 85.0%, which was significantly lower than 91.6% in the group of IT patients, р<0.0001. Conclusion. EPOCHA study shows that structure of antihypertensive therapy does not differ between ET and IT patients what is caused lack of medical supervision for patients with hypertension in real clinical practice.

History of introduction and distribution of common ragweed (Ambrosia artemisiifoliaL.) in the European part of the Russian Federation and in the Ukraine

EPPO Bulletin ◽  
2018 ◽  
Vol 48 (2) ◽  
pp. 266-273 ◽  
Author(s):  
A. N. Afonin ◽  
N. N. Luneva ◽  
Y. A. Fedorova ◽  
Yu. E. Kletchkovskiy ◽  
A. F. Chebanovskaya
Keyword(s):  
Russian Federation ◽  
European Part ◽  
Common Ragweed ◽  
History Of ◽  
The Russian Federation

QUALITATIVE INDICATORS OF HONEY FROM VARIOUS CLIMATIC ZONES

2018 ◽  
Vol 1 (3) ◽  
pp. 17-22
Author(s):  
Rodriguez Nancy Aguilar ◽  
◽  
S. V. Redkin ◽  
Y. G. Isaev ◽  
◽  
...  
Keyword(s):  
Russian Federation ◽  
Geographical Origin ◽  
Supply And Demand ◽  
Food Markets ◽  
Climatic Zones ◽  
The Russian Federation ◽  
The Republic ◽  
Natural Honey ◽  
Current Standards

Determining the quality of honey in procurement, trade organizations and food markets does not always correspond to the level of the requirements set by the current standards for this product, and the large gap between supply and demand for it, as well as the high cost of the product, often leads to various kinds of falsifications. The aim is to assess the quality of honey obtained in the Republic of Colombia and the Russian Federation on the basis of veterinary and sanitary researches. The article presents data on the veterinary and sanitary examination of natural honey from Colombia and Russia. The conducted study made it possible to establish that good organoleptic indices, the presence of pollen in honey samples and reliable establishment of the botanical and geographical origin of honey do not guarantee the quality of the product.

The image of the Russian Federation on the canvas of social research

2020 ◽  
Vol 18 (3) ◽  
pp. 115-140
Author(s):  
Natalia Sienko
Keyword(s):  
Russian Federation ◽  
Social Research ◽  
Saint Petersburg ◽  
Starting Point ◽  
The Russian Federation ◽  
Principal Analysis ◽  
Depth Interviews

The starting point for this article was research conducted from February to April 2017 and in January and February 2020. The purpose of the work is to show the image and position of the Russian Federation in the eyes of respondents who participated (incl. residents of Moscow, Arkhangelsk, Saint Petersburg and Vladivostok) in in-depth interviews conducted by the author. The principal analysis concerns the results obtained in 2017, and the specific changes observed three years after the initial surveys were completed.

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