In situ crosslinkable hydrogels for engineered cellular microenvironments

In situ crosslinkable hydrogels have been widely used as therapeutic implants and vehicles for a broad range of biomedical applications including tissue regenerative medicine because of their biocompatibility and easiness of encapsulation of cells or signaling molecules during hydrogel formation. Recently, these hydrogel materials have been widely utilized as an artificial extracellular matrix (aECM) because of its structural similarity with the native extracellular matrix (ECM) of the human body and its multi-tunable properties. Various synthetic, natural, and semisynthetic hydrogels have been developed as engineered cellular microenvironments by using various crosslinking strategies. In this review, we discuss how in situ forming hydrogels are being created with tunable physical, chemical, and biological properties. In particular, we focus on emerging techniques to apply advanced hydrogel materials for engineered cellular microenvironments.

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Introduction to In Situ Forming Hydrogels for Biomedical Applications

In-Situ Gelling Polymers - Series in BioEngineering ◽

10.1007/978-981-287-152-7_2 ◽

2014 ◽

pp. 5-35 ◽

Cited By ~ 10

Author(s):

Bogyu Choi ◽

Xian Jun Loh ◽

Aloysius Tan ◽

Chun Keat Loh ◽

Enyi Ye ◽

...

Keyword(s):

Biomedical Applications ◽

In Situ Forming ◽

In Situ Forming Hydrogels

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In Situ Forming Hydrogels Based on Clickable Star-PEG for Biomedical Applications

Eco-friendly and Smart Polymer Systems ◽

10.1007/978-3-030-45085-4_23 ◽

2020 ◽

pp. 92-95

Author(s):

Mohammad Hossein Ghanian ◽

Hamid Mirzadeh ◽

Hossein Baharvand

Keyword(s):

Biomedical Applications ◽

In Situ Forming ◽

In Situ Forming Hydrogels

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Regeneration of critical-sized defects, in a goat model, using a dextrin-based hydrogel associated with granular synthetic bone substitute

Regenerative Biomaterials ◽

10.1093/rb/rbaa036 ◽

2020 ◽

Author(s):

Isabel Pereira ◽

José Eduardo Pereira ◽

Luís Maltez ◽

Alexandra Rodrigues ◽

Catarina Rodrigues ◽

...

Keyword(s):

Bone Regeneration ◽

Bone Substitute ◽

Bone Substitutes ◽

Multipotent Mesenchymal Stromal Cells ◽

Micro Computed Tomography ◽

Synthetic Bone Substitute ◽

In Situ Forming ◽

Bioactive Agents ◽

In Situ Forming Hydrogels

Abstract The development of injectable bone substitutes (IBS) have obtained great importance in the bone regeneration field, as a strategy to reach hardly accessible defects using minimally invasive techniques and able to fit to irregular topographies. In this scenario, the association of injectable hydrogels and bone graft granules is emerging as a well-established trend. Particularly, in situ forming hydrogels have arisen as a new IBS generation. An in situ forming and injectable dextrin-based hydrogel (HG) was developed, aiming to act as a carrier of granular bone substitutes and bioactive agents. In this work, the HG was associated to a granular bone substitute (Bonelike®) and implanted in goat critical-sized calvarial defects (14 mm) for 3, 6 and 12 weeks. The results showed that HG improved the handling properties of the Bonelike® granules and did not affect its osteoconductive features, neither impairing the bone regeneration process. Human multipotent mesenchymal stromal cells from the umbilical cord, extracellular matrix hydrolysates and the pro-angiogenic peptide LLKKK18 were also combined with the IBS. These bioactive agents did not enhance the new bone formation significantly under the conditions tested, according to micro-computed tomography and histological analysis.

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Catechol-modified hyaluronic acid: in situ-forming hydrogels by auto-oxidation of catechol or photo-oxidation using visible light

Polymer Bulletin ◽

10.1007/s00289-017-1937-y ◽

2017 ◽

Vol 74 (10) ◽

pp. 4069-4085 ◽

Cited By ~ 8

Author(s):

Takeshi Sato ◽

Takao Aoyagi ◽

Mitsuhiro Ebara ◽

Rachel Auzély-Velty

Keyword(s):

Hyaluronic Acid ◽

Visible Light ◽

Photo Oxidation ◽

In Situ Forming ◽

In Situ Forming Hydrogels

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Recent Advances in the Cancer Bioimaging with Graphene Quantum Dots

Current Medicinal Chemistry ◽

10.2174/0929867324666170223154145 ◽

2018 ◽

Vol 25 (25) ◽

pp. 2876-2893 ◽

Cited By ~ 15

Author(s):

Keheng Li ◽

Xinna Zhao ◽

Gang Wei ◽

Zhiqiang Su

Keyword(s):

Quantum Dots ◽

Biomedical Applications ◽

Cell Imaging ◽

Graphene Quantum Dots ◽

Biological Properties ◽

Live Cells ◽

Physical Chemical ◽

Specific Cancer ◽

Good Biocompatibility ◽

Low Cytotoxicity

Fluorescent graphene quantum dots (GQDs) have attracted increasing interest in cancer bioimaging due to their stable photoluminescence (PL), high stability, low cytotoxicity, and good biocompatibility. In this review, we present the synthesis and chemical modification of GQDs firstly, and then introduce their unique physical, chemical, and biological properties like the absorption, PL, and cytotoxicity of GQDs. Finally and most importantly, the recent applications of GQDs in cancer bioimaging are demonstrated in detail, in which we focus on the biofunctionalization of GQDs for specific cancer cell imaging and real-time molecular imaging in live cells. We expect this work would provide valuable guides on the synthesis and modification of GQDs with adjustable properties for various biomedical applications in the future.

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