scholarly journals Hemato-Biochemical studies on Egyptian Buffaloes and Calves naturally infected with Foot and Mouth Disease Virus serotype SAT 2

2016 ◽  
Vol 73 (2) ◽  
pp. 230
Author(s):  
Essam A Mahmoud ◽  
Ahmed N.F Neamat-allah
Keyword(s):  
Clinical Signs ◽  
Foot And Mouth Disease ◽  
Viral Disease ◽  
Mouth Disease ◽  
Cardiac Markers ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Foot And Mouth ◽  
Infected Adult ◽  
Apparently Healthy

Foot and mouth disease (FMD) is a highly contagious viral disease of all cloven footed domestic and wild animals. This work was planned to study the different markers for diagnosis of FMDV serotype Sat2 in adult buffaloes and calves including clinical, hematological and biochemical examinations. A total number of sixty animals were divided into four groups. The first group was apparently healthy adult buffaloes, while the second was naturally infected adult buffaloes, a third group was apparently healthy suckling calves and finally the fourth group was naturally infected suckling calves. The recorded clinical signs were fever, salivation, loss of appetite, depression, lameness, blisters or vesicles, erosions and ulcers in the mucosa of the mouth, tongue, lips, gums, pharynx, palate and between the claws. Anemia, leucopenia, lymphopenia and monocytopenia were recorded in infected adult buffaloes and calves. Myocardial injury proved by presence of degenerated myocardial fibers and lymphocyte cell infiltration with a significant increase in cardiac markers like cardiac torponin I, CPK and LDH in addition to a significant hyperkalemia, hypocalcaemia and hypomagnesemia in buffaloe calves. Moreover, electrophoresis showed hyoproteinemia, hypoalbuminemia and hypoglobulinemia in infected animals. It could be concluded that the elevation of cardiac markers emphasized that FMD is more severe in young calves than adult animals. Therefore, it is recommended to evaluate the prognosis of FMD infection in calves by these markers.

scholarly journals The High Immunity Induced by the Virus-Like Particles of Foot-and-Mouth Disease Virus Serotype O

2021 ◽  
Vol 8 ◽  
Author(s):  
Yan Xiao ◽  
Suling Zhang ◽  
He Yan ◽  
Xiaolin Geng ◽  
Yanwei Wang ◽  
...  
Keyword(s):  
Foot And Mouth Disease ◽  
Viral Disease ◽  
Mouth Disease ◽  
Dependent Manner ◽  
Virus Like Particles ◽  
Serotype O ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Foot And Mouth ◽  
Cellular Immune

Foot-and-mouth disease (FMD), caused by FMD virus (FMDV), is a highly contagious and economically devastating viral disease of cloven-hoofed animals worldwide. In this study, the coexpression of small ubiquitin-like modifier (SUMO)–fused capsid proteins of FMDV serotype O by single plasmid in Escherichia coli was achieved with an optimal tandem permutation (VP0–VP3–VP1), showing a protein yield close to 1:1:1. After SUMO removal at a low level of protease activity (5 units), the assembled FMDV virus-like particles (VLPs) could expose multiple epitopes and have a size similar to the naive FMDV. Immunization of pigs with the FMDV VLPs could induce FMDV-specific humoral and cellular immune responses effectively, in a dose-dependent manner. These data suggested that the stable FMDV VLPs with multiple epitope exposure were effective for the induction of an immune response in pigs, which laid a foundation for the further development of the FMDV subunit vaccine.

scholarly journals Transgenic shRNA pigs reduce susceptibility to foot and mouth disease virus infection

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Shengwei Hu ◽  
Jun Qiao ◽  
Qiang Fu ◽  
Chuangfu Chen ◽  
Wei Ni ◽  
...  
Keyword(s):  
Disease Virus ◽  
Clinical Signs ◽  
Foot And Mouth Disease ◽  
Viral Disease ◽  
Mouth Disease ◽  
Swine Industry ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Interfering Rna

Foot-and-mouth disease virus (FMDV) is an economically devastating viral disease leading to a substantial loss to the swine industry worldwide. A novel alternative strategy is to develop pigs that are genetically resistant to infection. Here, we produce transgenic (TG) pigs that constitutively expressed FMDV-specific short interfering RNA (siRNA) derived from small hairpin RNA (shRNA). In vitro challenge of TG fibroblasts showed the shRNA suppressed viral growth. TG and non-TG pigs were challenged by intramuscular injection with 100 LD50 of FMDV. High fever, severe clinical signs of foot-and-mouth disease and typical histopathological changes were observed in all of the non-TG pigs but in none of the high-siRNA pigs. Our results show that TG shRNA can provide a viable tool for producing animals with enhanced resistance to FMDV.

scholarly journals Novel Viral Disease Control Strategy: Adenovirus Expressing Alpha Interferon Rapidly Protects Swine from Foot-and-Mouth Disease

2003 ◽  
Vol 77 (2) ◽  
pp. 1621-1625 ◽  
Author(s):  
Jarasvech Chinsangaram ◽  
Mauro P. Moraes ◽  
Marla Koster ◽  
Marvin J. Grubman
Keyword(s):  
Clinical Signs ◽  
Foot And Mouth Disease ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Viral Disease ◽  
Mouth Disease ◽  
Biologically Active ◽  
Nonstructural Proteins ◽  
Mouth Disease Virus ◽  
Foot And Mouth

ABSTRACT We have previously shown that replication of foot-and-mouth disease virus (FMDV) is highly sensitive to alpha/beta interferon (IFN-α/β). In the present study, we constructed recombinant, replication-defective human adenovirus type 5 vectors containing either porcine IFN-α or IFN-β (Ad5-pIFNα or Ad5-pIFNβ). We demonstrated that cells infected with these viruses express high levels of biologically active IFN. Swine inoculated with 109 PFU of a control Ad5 virus lacking the IFN gene and challenged 24 h later with FMDV developed typical signs of foot-and-mouth disease (FMD), including fever, vesicular lesions, and viremia. In contrast, swine inoculated with 109 PFU of Ad5-pIFNα were completely protected when challenged 24 h later with FMDV. These animals showed no clinical signs of FMD and no viremia and did not develop antibodies against viral nonstructural proteins, suggesting that complete protection from infection was achieved.

Antiviral potential of ivermectin against foot-and-mouth disease virus, serotype O, A and Asia-1

2021 ◽  
pp. 104914
Author(s):  
Zahra Naeem ◽  
Sohail Raza ◽  
Saba Afzal ◽  
Ali Ahmad Sheikh ◽  
Muhammad Muddassir Ali ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Serotype O ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Foot And Mouth

A recombinant protein-based ELISA for detecting antibodies to foot-and-mouth disease virus serotype Asia 1

2009 ◽  
Vol 159 (1) ◽  
pp. 112-118 ◽  
Author(s):  
Young-Joon Ko ◽  
Hye-Young Jeoung ◽  
Hyang-Sim Lee ◽  
Byung-Sik Chang ◽  
Seung-Min Hong ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Foot And Mouth ◽  
Serotype Asia 1

Evidence of recombination in a new isolate of foot-and-mouth disease virus serotype Asia 1

2009 ◽  
Vol 139 (1) ◽  
pp. 117-121 ◽  
Author(s):  
Kwang-Nyeong Lee ◽  
Jae-Ku Oem ◽  
Jong-Hyeon Park ◽  
Su-Mi Kim ◽  
Seo-Yong Lee ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Foot And Mouth ◽  
Serotype Asia 1

scholarly journals Analysis of Amino Acid Mutations of the Foot-and-Mouth Disease Virus Serotype O Using both Heparan Sulfate and JMJD6 Receptors

Viruses ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 1012 ◽  
Author(s):  
Gyeongmin Lee ◽  
Ji-Hyeon Hwang ◽  
Aro Kim ◽  
Jong-Hyeon Park ◽  
Min Ja Lee ◽  
...  
Keyword(s):  
Amino Acid ◽  
Heparan Sulfate ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Amino Acid Sequences ◽  
Cell Adaptation ◽  
Field Virus ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Foot And Mouth

Foot-and-mouth disease (FMD) is an economically devastating animal disease. Adapting the field virus to cells is critical to the vaccine production of FMD viruses (FMDV), and heparan sulfate (HS) and Jumonji C-domain-containing protein 6 (JMJD6) are alternative receptors of cell-adapted FMDV. We performed serial passages of FMDV O/SKR/Andong/2010, classified as the O/Mya-98 topotype/lineage and known as a highly virulent strain, to develop a vaccine seed virus. We traced changes in the amino acid sequences of the P1 region, plaque phenotypes, and the receptor usage of the viruses, and then structurally analyzed the mutations. VP3 H56R and D60G mutations were observed in viruses using the HS receptor and led to changes in the hydrogen bonding between VP3 56 and 60. A VP1 P208L mutation was observed in the virus using the JMJD6 receptor during cell adaptation, enabling the interaction with JMJD6 through the formation of a new hydrogen bond with JMJD6 residue 300. Furthermore, VP1 208 was near the VP1 95/96 amino acids, previously reported as critical mutations for JMJD6 receptor interactions. Thus, the mutation at VP1 208 could be critical for cell adaptation related to the JMJD6 receptor and may serve as a basis for mechanism studies on FMDV cell adaptation.

scholarly journals Emergence of novel lineage of foot‐and‐mouth disease virus serotype Asia1 BD‐18 (G‐IX) in Bangladesh

2019 ◽  
Vol 67 (2) ◽  
pp. 486-493 ◽  
Author(s):  
M. Rahmat Ali ◽  
A. S. M. Rubayet Ul Alam ◽  
Md. Al Amin ◽  
Mohammad Anwar Siddique ◽  
Munawar Sultana ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Virus Serotype ◽  
Foot And Mouth
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