Salinity Stress in Common Bean (Phaseolus vulgaris L.) Seed Germination

In this study, the effect of five different levels of salt (NaCl) on the germination of Phaseolus vulgaris L. seed was investigated. Laboratory experiment with completely randomized design comprising three replicates in Petri dishes was conducted at Gaziantep University Vocational School of Higher Education in Nurdagi to determine the salt effects on common bean germination. The germination of the cultivar (Tegmen) was studied using distilled water (control) and under osmotic potential of 0.3, 0.6, 0.9, 1.2, and 1.5 MPa NaCl. The results indicated that the Mean germination time (MGT), Germination index (GI), Coefficient of velocity of germination (CVG), Germination percentage (GP), and Seed vigor index (SVI) varied between 3.13 and 3.78 days, 6.88 and 3.93, 0.156 and 0.153, 90 and 60%, and 867.0 and 290.3, respectively. Significant differences were found among NaCl treatments in terms of GI, GP, and SVI. All the examined parameters were decreased with increasing NaCl concentration, except MGT. The max and min GI, GP, CVG, and SVI were observed at the control condition (0.0 MPa) and highest osmotic potential (1.5 MPa) of NaCl, respectively. Correlation coefficient between all possible combinations was estimated and the results indicated that MGT, GI, GP, CVG, and SVI had significant positive or negative correlation with each other.

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SEED GERMINATION AND RADICLE ESTABLISHMENT RELATED TO TYPE AND LEVEL OF SALT IN COMMON BEAN (PHASEOLUS VULGARIS L. VAR. DJEDIDA)

Cercetari Agronomice in Moldova ◽

10.46909/cerce-2019-0026 ◽

2019 ◽

Vol 52 (3) ◽

pp. 262-277

Author(s):

L.M. MANSOURI ◽

N. HELEILI ◽

Z.F. BOUKHATEM ◽

A. KHELOUFI

Keyword(s):

Phaseolus Vulgaris ◽

Seed Germination ◽

Common Bean ◽

Seedling Growth ◽

Dry Weight ◽

Germination Percentage ◽

Phaseolus Vulgaris L ◽

Randomized Design ◽

Dry Biomass ◽

Secondary Roots

Saline soils contain multiple types of salt, each of them may exert a different effect on seed germination and seedling growth. The aim of this study is to assess the effects of five types of salt on the seed germination and radicle establishment of common bean (Phaseolus vulgaris L. var. Djedida). The experiment was set out as a factorial experiment based on completely randomized design with four replications of ten seeds for the seed germination and twelve replicates for the seedling growth. We used five kinds of salts (NaCl, Na2SO4, CaCl2, CaCO3 and KCl) with concentrations of 0, 100, 200 and 300 mM. Seeds were incubated in Petri dishes at 25°C, in the dark, for 10 days. The results of analysis of variance indicated that the effects of salt types and concentrations, and their interaction effect were significant in all measured traits (P< 0.01). However, no significance effect was found on secondary roots number and seedlings dry biomass with CaCO3 treatment solution. According to the results, the inhibitory effects of the five salt types differed significantly. Indeed, germination of common bean seeds by various salts were in the order of NaCl > KCl > CaCO3 > Na2SO4 > CaCl2. However, the taproot length, the number of secondary roots and the seedlings dry weight by various salts were in the order of CaCO3 > KCl > NaCl > CaCl2 > Na2SO4. The effect of salt concentration was also obvious. The reduction in dry biomass of cotyledons is proportional to germination rates and to the development of seedlings in dry biomass and in size. Seeds of P. vulgaris var. Djedida were able to germinate under all concentrations of the various types of salt. The lowest final germination percentage (FGP) was obtained under 300 mM of all salts recording the following values: CaCO3 - 60%, NaCl - 60%, KCl - 52.5%, Na2SO4 - 50% and CaCl2 - 27.5%. During germination stage, the radicle emergence would be controlled by the environment osmolarity, while the later growth of the seedling would be limited by the reserve mobilization.

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EFFECTS OF SEED PRIMING WITH SODIUM CHLORIDE ON SEEDLING PERFORMANCE OF COMMON BEAN (PHASEOLUS VULGARIS L.) UNDER GREEN HOUSE CONDITION

International Journal of Research -GRANTHAALAYAH ◽

10.29121/granthaalayah.v4.i6.2016.2654 ◽

2016 ◽

Vol 4 (6) ◽

pp. 222-228

Author(s):

Roba Tufa ◽

Jima Nego

Keyword(s):

Sodium Chloride ◽

Phaseolus Vulgaris ◽

Common Bean ◽

Germination Rate ◽

Shoot Length ◽

Distilled Water ◽

Seedling Height ◽

Phaseolus Vulgaris L ◽

Vigor Index ◽

Nacl Concentration

This study was conducted at Jimma University College of Agriculture and Veterinary Medicine (JUCAVM) under controlled environmental conditions of greenhouse to determine the effects of different concentration of sodium chloride (NaCl) on germination and seedling growth of Phaseolus vulgaris L. Five concentrations of sodium chloride (o mol(distilled water), 0.1M, 0.2M,0.3M and 0.4M) with one control treatment of un-primed seeds were laid out in completely randomized design (CRD) with three replication. Data were collected on standard germination, Rate of germination, Seedling height, Shoot length, Root length and Vigor index. The result revealed that different NaCl concentrations statistically (p≤0.05) affected standard germination, Rate of germination, Seedling height, Shoot length and Vigor index I of common bean crop. The highest value of all these parameters were obtained from seed primed with 0.1M NaCl as well as when the seed primed with distilled water. However, the lowest values of these parameters were recorded on the seed primed with the highest concentration (0.4M) of NaCl and un-primed seed. More of the examined parameters were decreased with increasing of NaCl concentration. In conclusion, priming seed with 0.1M of NaCl concentration enhanced germination and seedling performance of common bean.

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