scholarly journals Physiological variation of irradiated red radish plants and their phylogenic relationship using SCoT and CDDP markers

2021 ◽  
Vol 49 (3) ◽  
pp. 12396
Author(s):  
Amina A. ALY ◽  
Noha E. ELIWA ◽  
Zeyad M. BORIK ◽  
Gehan SAFWAT
Keyword(s):  
Molecular Markers ◽  
Dose Rate ◽  
Dose Level ◽  
Gamma Rays ◽  
Irradiation Dose ◽  
Start Codon ◽  
Root Weight ◽  
Banding Patterns ◽  
Two Generations ◽  
Red Radish

Greenhouse experiment is carried out to explore the outcome of γ-radiation on physiological and genetic variation in red radish (Raphanus sativus) for two generations. Gamma rays from 60Co were used to penetrate red radish seeds with different dose levels (0.0, 10, 20, 40 and 80 Gy). Plants generated from irradiated seeds and from self-pollination of these plants, called M1 and M2 generations, respectively. Some morphological and physiological traits were then determined, and the genetic diversity of both generations was studied using Start Codon Targeted (SCoT) and Conserved DNA-Derived Polymorphism (CDDP) molecular markers.  All studied morphological traits (number of leaves/plants, leave height, root diameter, and root weight) were steadily improved by raising irradiation dose rate, reaching a cumulative raise at the irradiation doe level 40 Gy and decreased at dose level 80 Gy. Photosynthetic pigments of red radish plants released a notable increase by increasing gamma rays dose level for chlorophyll (a), chlorophyll (b) and carotenoids for 40 Gy dose rate. Proline content was elevated proportionally to the irradiation dose level, with the greatest increase seen at dose level of 80 Gy. Moreover, phytochemical screening was detected for the both two generations. Fourteen SCoT primers generated a total number of banding patterns of 194 with average 13.86 and the primer SCoT-33 released the highest number banding patterns (21). The percentage mean of polymorphism for all the SCoT primers was 74.66% and was 66.49 and 63.74% for M1 and M2 respectively. Furthermore, fifteen CDDP primers generated a total number of banding patterns of 186 and the primer CDDP-5 relieved the highest number of banding patterns (20). The percentage mean of polymorphism for all the CDDP primers was 73.41% and was 64.38 and 65.91% for M1 and M2 respectively. It could be concluded that gamma irradiation exhibited an appropriate variation in red radish M1 and M2 which was detected by SCoT and CDDP molecular markers.

Assessment of Absorbed Irradiation Dose Rate 137Cs by Emerged Plants in Oligotrophic and Eutrophic Water Bodies

2019 ◽  
Vol 55 (5) ◽  
pp. 100-106
Author(s):  
Ye. N. Volkova ◽  
V. V. Belyayev ◽  
S. P. Prishlyak ◽  
A. A. Parkhomenko
Keyword(s):  
Dose Rate ◽  
Irradiation Dose ◽  
Water Bodies ◽  
Eutrophic Water ◽  
Irradiation Dose Rate

scholarly journals Development of Molecular Markers for Predicting Radish (Raphanus sativus) Flesh Color Based on Polymorphisms in the RsTT8 Gene

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1386
Author(s):  
Soyun Kim ◽  
Keunho Yun ◽  
Han Yong Park ◽  
Ju Young Ahn ◽  
Ju Yeon Yang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Raphanus Sativus ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Cosmetic Industry ◽  
Natural Colorants ◽  
Health Promoting ◽  
Primer Sets ◽  
Red Radish ◽  
Simple Sequence

Red radish (Raphanus sativus L.) cultivars are a rich source of health-promoting anthocyanins and are considered a potential source of natural colorants used in the cosmetic industry. However, the development of red radish cultivars via conventional breeding is very difficult, given the unusual inheritance of the anthocyanin accumulation trait in radishes. Therefore, molecular markers linked with radish color are needed to facilitate radish breeding. Here, we characterized the RsTT8 gene isolated from four radish genotypes with different skin and flesh colors. Sequence analysis of RsTT8 revealed a large number of polymorphisms, including insertion/deletions (InDels), single nucleotide polymorphisms (SNPs), and simple sequence repeats (SSRs), between the red-fleshed and white-fleshed radish cultivars. To develop molecular markers on the basis of these polymorphisms for discriminating between radish genotypes with different colored flesh tissues, we designed four primer sets specific to the RsTT8 promoter, InDel, SSR, and WD40/acidic domain (WD/AD), and tested these primers on a diverse collection of radish lines. Except for the SSR-specific primer set, all primer sets successfully discriminated between red-fleshed and white-fleshed radish lines. Thus, we developed three molecular markers that can be efficiently used for breeding red-fleshed radish cultivars.

scholarly journals Study on the immuno stimulation of radiation degraded β-glucan in swiss mice

2015 ◽  
Vol 5 (4) ◽  
pp. 9-15
Author(s):  
Thanh Long Nguyen ◽  
Quang Luan Le
Keyword(s):  
Cell Wall ◽  
Immune System ◽  
Gamma Irradiation ◽  
Gamma Rays ◽  
Irradiation Dose ◽  
Functional Foods ◽  
Water Soluble ◽  
Molecular Weights ◽  
Swiss Mice ◽  
Stimulation Of

The mixtures β-glucan extracted from the yeast cell wall were irradiated under gamma rays from a Co-60 source at doses of 100, 200 and 300 kGy in order to prepare water-soluble β-glucan. Yields of the water soluble β-glucan produced are 25.9, 49.1, 66.71%, and their molecular weights (Mw) are 30.5, 24.9 and 10.8 kDa, respectively. There are no any new peak in the IR spectra of the irradiated β-glucan samples, but the intensity ratio between the peaks at wavenumber of 1156 cm-1 (assigned to C-O-C bond) and of 1040 cm-1 (assigned to C-C bond) in glycosidic linkages was reduced with irradiation dose. These results revealed that gamma irradiation did not cause any change in the β-glucan structure except the scissions of glycosidic linkages. In this study, immuno stimulation of the irradiated b-glucan was also investigated for the Swiss mice. After 28 days supplying with the irradiated b-glucan, not only cellular indexes (white blood cell, neutrophils and lymphocytes counts), but also humoral  immunity indexes (IgA and IgM) of the mice significantly increased and the highest effects was obtained for the mice supplied with the oligoβ-glucan prepared by gamma irradiation at 200 kGy. Thus, the water soluble oligoβ-glucan with Mw ~ 24.9 kDa prepared by gamma radiation much stimulated the natural immune system (non-specific immunity) in mice including both the cellular and humoral immunities. Particularly, the irradiated b-glucan is a very promising product for preparation of functional foods aiming at cancer prevention.

Late Effects of Fast Neutrons and Gamma-Rays in Mice as Influenced by the Dose Rate of Irradiation: Induction of Neoplasia

1970 ◽  
Vol 41 (3) ◽  
pp. 467 ◽  
Author(s):  
A. C. Upton ◽  
M. L. Randolph ◽  
J. W. Conklin ◽  
M. A. Kastenbaum ◽  
M. Slater ◽  
...  
Keyword(s):  
Dose Rate ◽  
Gamma Rays ◽  
Late Effects ◽  
Fast Neutrons
Sign in / Sign up

Export Citation Format

Share Document