scholarly journals Relative luminosity in the plus maze upon the exploratory behaviour of female Wistar rats

2015 ◽  
Vol 73 (7) ◽  
pp. 601-606 ◽  
Author(s):  
Diana Yae Sakae ◽  
Thiago Mamoru Sakae ◽  
Marta Aparecida Paschoalini ◽  
Moacir Serralvo Faria
Keyword(s):  
Regression Analysis ◽  
Multiple Regression Analysis ◽  
Estrous Cycle ◽  
Predictive Validity ◽  
Wistar Rats ◽  
Exploratory Behaviour ◽  
Cycle Phase ◽  
Plus Maze ◽  
Female Wistar Rats ◽  
Relative Luminosity

Objective This study evaluated the provision of two configuration of the Elevated Pluz-Maze (EPM) by analizing the exploratory behaviour of female Wistar rats in different phases of the estrous cycle in EPMs with different gradients of luminosity between the open and enclosed arms (O/E∆Lux).Methods Female Wistar rats were treated with Midazolam (MDZ, 1.0 mg.kg-1) and were tested for their exploratory behaviour in either the EPM 10 O/E∆Lux or EPM 96 O/E∆Lux.Results A multiple regression analysis indicated that the O/E∆Lux is negatively associated with the %Open arm entries and %Open arm time, suggesting that as O/E∆Lux increases, the open arm exploration decreases. The estrous cycle phase did not influence the open-arm exploration in either EPM. MDZ- induced anxiolysis was detected in 96 O/E∆Lux EPM in all phases of the EC.Discussion Results of this study suggest the importance of the O/E∆Lux to establish the arm preference in the EPM, and to preserve the predictive validity of the EPM.

scholarly journals The fertility assessment of normal cyclic Wistar rats following the administration of methanolic extract of Portulaca oleracea: an experimental study

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Izuchukwu Azuka Okafor ◽  
Uchenna Somtochukwu Nnamah ◽  
Jude Nnaka
Keyword(s):  
Estrous Cycle ◽  
Adult Female ◽  
Wistar Rats ◽  
Major Effect ◽  
Reproductive Hormones ◽  
Portulaca Oleracea ◽  
High Dose ◽  
Methanolic Extracts ◽  
Cycle Arrest ◽  
Female Wistar Rats

Abstract Background Purslane is a widely distributed shrub used for the treatment of different ailments. The increasing reproductive complications associated with herbal treatments have led to the need to critically evaluate the safety and/or reproductive potentials of commonly used plant extracts. This study investigated the reproductive effect of methanolic extracts of Portulaca oleracea (MEPO) in adult female Wistar rats. Results Group C showed a significant decrease both in relative ovarian weight (p = 0.000), and relative uterine weight (p = 0.037), when compared with the control. There were no significant (p ˃ 0.05) changes in the levels of follicle-stimulating hormone, luteinizing hormone, progesterone, and estradiol. When compared to the control, groups B and C showed abnormal estrous cycle and cycle arrest especially at the metestrus phase with mild congestion of a few blood vessels in the ovary and uterus. Conclusions MEPO may possess some anti-fertility effect, as it disrupts the estrous cycle of adult female Wistar rats; although it has no major effect on the reproductive hormones, uterus, and ovarian histology of adult female Wistar rats. However, high dose consumption should be taken with precaution.

scholarly journals Montanoa frutescensandMontanoa grandifloraExtracts Reduce Anxiety-Like Behavior during the Metestrus-Diestrus Phase of the Ovarian Cycle in Wistar Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Juan Francisco Rodríguez-Landa ◽  
Julio Vicente-Serna ◽  
Luis Alfredo Rodríguez-Blanco ◽  
María de Jesús Rovirosa-Hernández ◽  
Francisco García-Orduña ◽  
...  
Keyword(s):  
Open Field ◽  
Field Test ◽  
Wistar Rats ◽  
Open Field Test ◽  
Ovarian Cycle ◽  
Male Rats ◽  
Cycle Phase ◽  
Medicinal Use ◽  
Plus Maze ◽  
Estrus Phase

In previous studies, the anxiolytic-like effects ofMontanoa tomentosaandMontanoa frutescenswere reported in male rats, but the potential anxiolytic-like effects ofMontanoaplants during the different phases of the ovarian cycle in rats remain to be explored. The anxiolytic-like effects of the aqueous crude extracts ofM. frutescens(25 and 50 mg/kg) andM. grandiflora(25 and 50 mg/kg) in the elevated plus maze were investigated in Wistar rats during the estrous cycle and compared with 2 mg/kg diazepam as a reference anxiolytic drug. To investigate any motor effect (i.e., hyperactivity, no changes, or hypoactivity) associated with the treatments, the rats were evaluated in the open field test. TheM. frutescens(25 and 50 mg/kg) andM. grandiflora(50 mg/kg) extracts exerted anxiolytic-like effects during the metestrus-diestrus phase, similar to diazepam, without disrupting spontaneous motor activity. No significant effects of the extracts were detected in either behavioral test during the proestrus-estrus phase, whereas diazepam produced motor hypoactivity in the open field test. These results indicate that theM. frutescensandM. grandifloraextracts possess anxiolytic-like effects that depend on the ovarian cycle phase, supporting the Mexican ancient medicinal use of these plants to ameliorate anxiety disorders.

scholarly journals Effect of Penicillin on Reproductive Function in Female Wistar Rats

2021 ◽  
Vol 71 (2) ◽  
Author(s):  
Oyedeji K.O ◽  
Momoh R.O ◽  
Oderinde Gbenga
Keyword(s):  
Estrous Cycle ◽  
Wistar Rats ◽  
Reproductive Function ◽  
Daily Basis ◽  
Female Rats ◽  
Histopathological Study ◽  
Fertility Effect ◽  
Female Wistar Rats ◽  
Student’S T ◽  
Histopathological Studies

This study was designed to investigate the effect of penicillin on reproductive function in female Wistar rats. Fifteen female rats (120 – 160 g) were used for the estrous cycle and histopathological studies. Penicillin (17.14 mg/kg) was administered orally on daily basis for 21 and 50 days respectively for the estrous cycle and histological studies. Estrous cycle was carried out using the technique of Marcondes et al., histologies of the ovaries and uteri were also carried out. Data were analysed using descriptive statistics and student’s t-test at p=0.05. Treatment of rats for 21 days with penicillin (17.14 mg/kg) produced significant (p<0.05) increments in the estrous and metestrous phases as well as a significant (p<0.05) reduction in the proestrous phase of the estrous cycle relative to their respective controls. The histopathological study presented with a moderate endometrial congestion. It can therefore be concluded that penicillin probably has a pro-fertility effect with a moderate deleterious effect on the uteri at histological level in female Wistar rats.

scholarly journals Gossypol Promotes Degeneration of Ovarian Follicles in Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Ivana Cristina Nunes Gadelha ◽  
Michelly Fernandes de Macedo ◽  
Sílvia Catarina Salgado Oloris ◽  
Marilia Martins Melo ◽  
Benito Soto-Blanco
Keyword(s):  
Estrous Cycle ◽  
Wistar Rats ◽  
Serum Levels ◽  
Ovarian Follicles ◽  
The Other ◽  
Control Group ◽  
Stages Of Development ◽  
Hormone Levels ◽  
Stage Of Development ◽  
Female Wistar Rats

The present study aimed to determine if gossypol interferes with ovarian follicles in rats. Twenty-four female Wistar rats were assigned to two equal groups: one control group and the other dosed with gossypol (25 mg/kg/day, subcutaneously) for 15 days. Ovarian follicles were histologically classified according to the stage of development and as normal or atretic. Gossypol treatment reduced the length of estrous with an increase in the duration of the diestrus phase. This compound was responsible for reduced serum levels of T4 and progesterone. Treatment with gossypol was responsible for a significant reduction in the number of normal ovarian follicles and a significant increase in the number of atretic follicles, both in all stages of development. Thus, treatment of rats with gossypol was responsible for reduction in the number of viable follicles and changes in hormone levels that resulted in interference of the estrous cycle.

Estrous-cycle-dependent Variation in Orthodontic Tooth Movement

2002 ◽  
Vol 81 (6) ◽  
pp. 406-410 ◽  
Author(s):  
N. Haruyama ◽  
K. Igarashi ◽  
S. Saeki ◽  
M. Otsuka-Isoya ◽  
H. Shinoda ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Wistar Rats ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Serum Estradiol ◽  
Serum Trap ◽  
Female Wistar Rats ◽  
Cycle Dependent ◽  
Cyclic Changes ◽  
Estradiol Levels

Sex hormones, including estradiol, play important physiological roles in bone metabolism. The purpose of this study was to investigate whether there is estrous-cycle-dependent variation in orthodontic tooth movement, and, if so, to determine the mechanism. Ten-week-old female Wistar rats were used. They received repeated orthodontic force during specific phases in the estrous cycle. Tooth movement in animals that received force principally in estrus was about 33% greater than that in animals that received such force principally in pro-estrus (p < 0.05). Serum estradiol levels also varied according to the estrous cycle, with a peak during pro-estrus and a nadir during estrus, and were inversely related to tooth movement. Furthermore, there were negative correlations between estradiol and both serum TRAP activity and pyridinoline (r = -0.42, p < 0.05; r = -0.59, p < 0.001). These results suggest that cyclic changes in the estradiol level may be associated with the estrous-cycle-dependent variation in tooth movement through its effects on bone resorption.

The Validation of a Developmental Scale for Seriation

1985 ◽  
Vol 45 (2) ◽  
pp. 321-328 ◽  
Author(s):  
Johannes Kingma ◽  
Franciska L. Loth
Keyword(s):  
Regression Analysis ◽  
Multiple Regression Analysis ◽  
Predictive Validity ◽  
Number Line ◽  
Stepwise Multiple Regression ◽  
Stepwise Multiple Regression Analysis ◽  
Developmental Scale ◽  
Explained Variance ◽  
Criterion Variables ◽  
Selection Of

This study was concerned with the validation of the developmental Mokken scale for seriation. It was shown that the observed scale was invariant for different samples from previous research. A high correlation of .89 was found between the total score of the items on the scale and number line comprehension task (criterion variables). Stepwise multiple regression analysis revealed that a selection of two seriation tasks on the scale accounted for 76.4% of the explained variance, whereas the remaining tasks on the scale did not add significantly to the predictive validity. It was concluded that these two seriation tasks might be used for diagnostic purposes, whereas the found seriation scale consisting of six seriation tasks might be employed for the evaluation of seriation development.

scholarly journals Effect of Artesunate on Reproductive Function in Female Wistar Rats

2021 ◽  
Vol 71 (2) ◽  
Author(s):  
Oyedeji K.O
Keyword(s):  
Estrous Cycle ◽  
Wistar Rats ◽  
Reproductive Function ◽  
Daily Basis ◽  
Female Rats ◽  
Histopathological Study ◽  
Fertility Effect ◽  
Female Wistar Rats ◽  
Student’S T ◽  
Histopathological Studies

This study was designed to investigate the effect of artesunate on reproductive function in female Wistar rats. Fifteen female rats (120 – 160 g) were used for the estrous cycle and histopathological studies. Artesunate (1.43 mg/kg) was administered orally on daily basis for 21 and 50 days respectively for the estrous cycle and histological studies. Estrous cycle was carried out using the technique of Marcondes et al., histologies of the ovaries and uteri were also carried out. Data were analysed using descriptive statistics and student’s t-test at p=0.05. Treatment of rats for 21 days with artesunate (1.43 mg/kg) produced significant (p<0.05) increments in the estrous and metestrous phases as well as a significant (p<0.05) reduction in the proestrous phase of the estrous cycle relative to their respective controls. The histopathological study presented with ovarian medullar that is severely congested (hemorrhagic) including expanded lumen of the endometrial glands. It can therefore be concluded that artesunate probably has pro-fertility effect with deleterious effect on the ovaries at histological level in female Wistar rats.

scholarly journals Effect of Methotrexate on Reproductive Function in Female Wistar Rats

2021 ◽  
Vol 71 (2) ◽  
Author(s):  
Oyedeji K.O ◽  
Nwume B. E ◽  
Zachariah Richard
Keyword(s):  
Estrous Cycle ◽  
Wistar Rats ◽  
Reproductive Function ◽  
Daily Basis ◽  
Female Rats ◽  
Histopathological Study ◽  
Fertility Effect ◽  
Female Wistar Rats ◽  
Student’S T ◽  
Histopathological Studies

This study was designed to investigate the effect of methotrexate on reproductive function in female Wistar rats. Eighteen female rats (120 – 160 g) were used for the estrous cycle and histopathological studies. Methotrexate (0.071 mg/kg) was administered orally on daily basis for 21 and 50 days respectively for the estrous cycle and histological studies. Estrous cycle was carried out using the technique of Marcondes et al., histologies of the ovaries and uteri were also carried out. Data were analysed using descriptive statistics and student’s t-test at p=0.05. Treatment of rats for 21 days with methotrexate (0.071 mg/kg) produced significant (p<0.05) reductions in the proestrous and estrous phase of the estrous cycle as well as significant (p<0.05) increments in the metestrous and diestrous phases of the estrous cycle relative to their respective controls. The histopathological study revealed that treatment of rats with methotrexate (0.071 mg/kg) for 50 days presented with ovarian medullary part that is congested and oedematous as well as with multiple foci of hemorrhage within the endometria. It can therefore be concluded that that methotrexate probably has dominant pro-fertility effect, but also probably induced deleterious effects on the ovaries and uteri in female Wistar rats.

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