scholarly journals MOLECULAR CHARACTERIZATION OF JABOTICABA TREE GENOTYPES LOCATED IN THE MUNICIPALITIES OF RECÔNCAVO OF BAHIA

2016 ◽  
Vol 38 (3) ◽  
Author(s):  
ELAINE SILVA DA CRUZ ◽  
ANA CRISTINA VELLO LOYOLA DANTAS ◽  
CÁTIA DIAS DO CARMO ◽  
LUCIMÁRIO PEREIRA BASTOS
Keyword(s):  
Genetic Divergence ◽  
Polymorphic Information Content ◽  
Issr Markers ◽  
Fruit Tree ◽  
Resolution Power ◽  
Inter Simple Sequence Repeats ◽  
Native Populations ◽  
Simple Sequence ◽  
Issr Primers

ABSTRACT The jaboticaba tree (Plinia sp.) is a native fruit tree belonging to the Myrtaceae family, which occurs spontaneously throughout Brazil. The estimation of genetic divergence among genotypes of native populations can be useful for conservation and knowledge of available genetic resources, in the formation of gene banks and breeding species. In order to evaluate the genetic diversity of jaboticaba trees identified in the Bahia Reconcavo municipalities, 35 genotypes were characterized by ISSR primers (Inter Simple Sequence Repeats). The 18 primers generated a total of 463 amplicons, and the number of initiator fragments ranged from 14 to 36, with an average of 25.72% and 99.65% polymorphism. Polymorphic information content ranged from 0.13 to 0.33 with a mean of 0.22 and the marker resolution power from 4.34 to 15.77, with an average of 8.67. Multivariate analysis allowed the formation of five groups of genetic divergence, where the longest distance was 0.97 between JCA6 and JSF8 genotypes and the lower of JMT2 and JCA1 (0.11). From the use of ISSR markers it is possible to check the variability between genotypes on the evaluated jaboticaba trees.

Characterization of Italian populations ofLoliumspp. resistant and susceptible to diclofop by inter simple sequence repeat

Weed Science ◽  
2004 ◽  
Vol 52 (4) ◽  
pp. 554-563 ◽  
Author(s):  
Giovanni Dinelli ◽  
Alessandra Bonetti ◽  
Ilaria Marotti ◽  
Maurizio Minelli ◽  
Pietro Catizone
Keyword(s):  
Potential Role ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Intergeneric Hybridization ◽  
Banding Patterns ◽  
Inter Simple Sequence Repeats ◽  
Range Of Variation ◽  
Simple Sequence

Three ItalianLoliumweed populations, one susceptible and two resistant to diclofop, were characterized by the technique of inter simple sequence repeats (ISSR). The goal of this study was to taxonomically identify theseLoliumpopulations as well as to evaluate evidence for introgression of ISSR fragments fromFestucaand the potential role of this introgression in the diclofop response. ISSR analysis confirmed the genomic background of the weed populations to be consistent with that ofLolium. However, the great range of variation in ISSR banding patterns highlighted that the three ryegrass accessions are mixed populations made up of individuals resulting presumably from intrageneric and intergeneric hybridization in theLolium–Festucacomplex. TwoFestucagenus-discriminating and 20Festucaspecies-discriminating ISSR markers were screened among all the three ryegrass populations. The resistant Tuscania population carried the highest percentage ofFestucagenome (16.8%) followed by the resistant Roma (13.6%) and susceptible Vetralla (7.6%) populations. On the basis of these data some influence ofFestucagenome in diclofop resistance levels of studied ryegrass populations could be hypothesized.

scholarly journals Characterization of Aegilops kotschyi Boiss. x Triticum aestivum L. hybrid lines

2014 ◽  
Vol 66 (4) ◽  
pp. 109-120 ◽  
Author(s):  
Roman Prażak ◽  
Edyta Paczos-Grzęda
Keyword(s):  
Triticum Aestivum ◽  
Zinc Content ◽  
Triticum Aestivum L ◽  
Issr Markers ◽  
Inter Simple Sequence Repeats ◽  
Iron And Zinc ◽  
Germplasm Analysis ◽  
Hybrid Lines ◽  
Simple Sequence

A study of four F5 and one BC1F1 <em>Aegilops kotschyi </em>Boiss. x <em>Triticum aestivum </em>L. hybrid lines was conducted to determine their quantitative morphological and qualitative features as well as a molecular investigation was carried out. Observations of ten quantitative traits showed that the F5 hybrid lines exhibited intermediate values between <em>Ae. kotschyi </em>Boiss. and <em>T. aestivum </em>L., or had similar traits to one of the parents. These hybrid lines had a significantly lower number and weight of grains per main spike, main spike fertility and 1000-grain weight than <em>T. aestivum </em>L. cv. ‘Rusałka’. The BC1F1 hybrid line was characterized by wheat-like fertility and phenotype. The F5 hybrid lines were characterized by much higher variability of the analysed morphological traits than <em>T. aestivum </em>L. cv. ‘Rusałka’. Grains of the hybrid lines had higher protein and micronutrient (iron and zinc) content than wheat grains. The presence of DNA fragments specific to <em>Ae. kotschyi </em>Boiss. in the genotypes of the hybrid lines was confirmed by seven ISSR (Inter Simple Sequence Repeats) molecular markers. Two ISSR markers – ISSR23690 and ISSR33650 – were the most effective for germplasm analysis of the hybrid lines. The analysed lines can become a source material for improvement of common wheat <em>T. aestivum </em>L. in crossing programs.

Phylogenetic relationships between Leymus and related diploid Triticeae species revealed by ISSR markers

Biologia ◽  
2014 ◽  
Vol 69 (8) ◽  
Author(s):  
Guo-Ye Guo ◽  
Rui-Wu Yang ◽  
Chun-Bang Ding ◽  
Li Zhang ◽  
Yong-Hong Zhou ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Relationships ◽  
Diploid Species ◽  
Issr Markers ◽  
Similarity Coefficients ◽  
Triticeae Species ◽  
Inter Simple Sequence Repeats ◽  
Great Genetic Diversity ◽  
Simple Sequence ◽  
Issr Primers

AbstractTo investigate the genetic diversity and phylogenetic relationships between polyploid Leymus and related diploid species of the Triticeae tribe, inter-simple sequence repeats (ISSR) markers was used to analyze 41 Leymus accessions representing 22 species and 2 subspecies, together with Pseudoroegneria stipifolia (St), Psathyrostachys fragilis (Ns), Australopyrum retrofractum (W), Hordeum bogdanii, H. chilense (H) and Lophopyrum elongatum (Ee). A total of 376 clear and reproducible DNA fragments were amplified by 29 ISSR primers, among which 368 (97.87%) fragments were found to be polymorphic. 8–18 polymorphic bands were amplified by each polymorphic primer, with an average of 12.69 bands. The data of 376 ISSR bands were used to generate Nei’s similarity coefficients and to construct a dendrogram by means of UPGMA. The similarity coefficients data suggested great genetic diversity in genus Leymus and related diploid Triticeae species, the genetic diversity among the different species more abundant than that of the different accessions. The dendrogram and principal coordinate analysis showed explicit interspecific relationships and demonstrated close phylogenetic relationships between Leymus species and Psathyrostachys.

scholarly journals Genetic diversity and relationships among Hypericum L. species by ISSR Markers: A high value medicinal plant from Northern of Iran

Caryologia ◽  
2021 ◽  
Vol 74 (1) ◽  
pp. 97-107
Author(s):  
Shuyan Ma ◽  
Majid Khayatnezhad ◽  
Amir Abbas Minaeifar
Keyword(s):  
Hypericum Perforatum ◽  
High Capacity ◽  
Molecular Data ◽  
Issr Markers ◽  
Herbal Preparations ◽  
Inter Simple Sequence Repeats ◽  
Hypericum Perforatum L ◽  
Simple Sequence ◽  
Hypericum Species ◽  
Issr Primers

Hypericum L. species are generally known locally in Iran with the names “Hofariqun” which Ebn Sina (or Bo Ali Sina) called it. Plants of the genus Hypericum have traditionally been used as medicinal plants in various parts of the world. Hypericum perforatum L. is the source to one of the most manufactured and used herbal preparations in recent years, especially as a mild antidepressant. Therefore, due to the importance of these plant species, we performed a molecular data for this species. For this study, we used 175 randomly collected plants from 17 species in 9 provinces. Amplification of genomic DNA using 10 primers produced 141 bands, of which 127 were polymorphic (95.78%). The obtained high average PIC and MI values revealed high capacity of ISSR primers to detect polymorphic loci among Hypericum species. The genetic similarities of 17 collections were estimated from 0.617 to 0.911. According to Inter-Simple sequence repeats (ISSR) markers analysis, H. androsaemum and H. hirtellum had the lowest similarity and the species of H. perforaturm and H. triquetrifolium had the highest similarity. The aims of present study are: 1) can ISSR markers identify Hypericum species, 2) what is the genetic structure of these taxa in Iran, and 3) to investigate the species inter-relationship? The present study revealed that ISSR markers can identify the species. 

scholarly journals Morphoagronomic and molecular characterization of ornamental passion fruit cultivars

2017 ◽  
Vol 52 (10) ◽  
pp. 849-860 ◽  
Author(s):  
Kenia Gracielle da Fonseca ◽  
Fábio Gelape Faleiro ◽  
Nilton Tadeu Vilela Junqueira ◽  
Mariana Barth ◽  
Nelson Pires Feldberg
Keyword(s):  
Molecular Markers ◽  
Simple Sequence Repeats ◽  
Multivariate Analyses ◽  
Random Amplified Polymorphic Dna ◽  
Issr Markers ◽  
Passion Fruit ◽  
Validation Rate ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

Abstract: The objective of this work was to validate the morphoagronomic descriptors used in the protection processes of plant cultivars in Brazil, by characterizing six cultivars of ornamental passion fruit. The BRS Rubiflora, BRS Rosea Púrpura, BRS Céu do Cerrado, BRS Roseflora, BRS Estrela do Cerrado, and BRS Pérola do Cerrado cultivars, 33 morphoagronomic descriptors, and two molecular markers were used. The categorical morphoagronomic descriptors were analyzed by frequency distribution and multivariate analyses. The quantitative morphoagronomic descriptors were subjected to the analysis of variance and to the comparison of the means of each cultivar. The random amplified polymorphic DNA (RAPD) and the inter-simple sequence repeats (ISSR) markers were used for molecular analysis. A high-validation rate was observed for the morphoagronomic descriptors used in the protection of plant cultivars. The analyses of variance showed significant differences between the quantitative descriptors, and the molecular markers confirmed the genetic differences among the cultivars. There was a high correlation between the calculated distances based on the categorical morphoagronomic descriptors and molecular markers. The morphoagronomic descriptors and molecular markers are useful and complementary for the characterization and differentiation of cultivars.

scholarly journals Morphological and Molecular Characterization of Iranian Wild Blackberry Species Using Multivariate Statistical Analysis and Inter-Simple Sequence Repeats (ISSR) Markers 

2021 ◽  
Author(s):  
Ali Gharaghani ◽  
Mehdi Garazhian ◽  
Saeid Eshghi ◽  
Ahmad Tahmasebi
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeats ◽  
Morphological Traits ◽  
Diversity Index ◽  
Issr Markers ◽  
Multivariate Statistical ◽  
Principal Coordinates ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence ◽  
Issr Primers

Abstract This study was carried out to estimate the genetic diversity and relationships of 74 Iranian blackberry genotypes assigned to 5 different species using inter-simple sequence repeats (ISSR) marker analysis and morphological trait characterization. Sixteen traits including phenological, vegetative and reproductive attributes were recorded, and 10 ISSR primers were screened. Results showed that yield and leaf width have the highest and lowest genetic diversity, (diversity index = 62.57 and 13.74), respectively. Flowering and ripening date recorded as traits having the strongest correlations (r = 0.98). The selected 10 ISSR primers produced a total of 161 amplified fragments (200 to 3500 bp) of which 113 were polymorphic. The highest, lowest and average PIC values were 0.53, 0.38 and 0.44, respectively. Principle component analysis (PCA) based on morphological traits showed that the first six components explained 84.9% of the variations of traits studied, whilst the principal coordinate analysis (PCoA) based on ISSR data implied the first eight principal coordinates explained 67.06% of the total variation. Cluster analysis based on morphological traits and ISSR data classified all genotypes into two and three major groups, respectively, and the distribution pattern of genotypes was mainly based on species and the geographic origins.

scholarly journals Molecular identification and genetic relationships among Alcea (Malvaceae) species by ISSR Markers: A high value medicinal plant

Caryologia ◽  
2021 ◽  
Vol 74 (3) ◽  
pp. 65-75
Author(s):  
Jinxin Cheng ◽  
Dingyu Hu ◽  
Yaran Liu ◽  
Zetian Zhang ◽  
Majid Khayatnezhad
Keyword(s):  
Genetic Relationships ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Issr Markers ◽  
Great Capacity ◽  
Inter Simple Sequence Repeats ◽  
Plant Recognition ◽  
The Family ◽  
Simple Sequence ◽  
Issr Primers

Alcea L. is one of the largest genera of Malvaceae family with nearly 70 species worldwide mainly distributed in SW Asia. According to the latest revision of the family, it is represented by 34 species in the Flora of Iran, among them, 15 species are endemic. It is tough to accurate germplasm/ plant recognition by using morphological characteristics because of its propagation, growing and using. We conducted a molecular data analysis on these plant species due to their importance. We examined 156 plants from 14 species in 16 regions that were selected randomly for this investigation. It has been 119 polymorphic bands (94.33%) were resulted from 128 bands of 10 primers in amplification of genomic DNA. ISSR primers have a great capacity to detect polymorphic loci among Alcea species, as evidenced by the high average PIC and MI values found. The genetic similarity of 14 species was calculated and ranged between 0.635 to 0.990. Inter-Simple sequence repeats (ISSR) markers research revealed that Alcea tarica Pakravan & Ghahreman and Alcea kopetdaghensis lljin had the least similarity, while Alcea semnanica Pakravan and Alcea mazandaranica Pakravan & Ghahreman had the most. The current study attempts to answer three questions: 1) can ISSR markers identify Alcea species? 2) what is the genetic structure of these taxa in Iran? and 3) what is the inter-relationship between these taxa? The current study discovered that ISSR markers can be used to identify species.

scholarly journals Genetic diversity and relationships among salvia species by ISSR markers

Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 559-574
Author(s):  
Zhao Yanpeng ◽  
Wang Hongmei ◽  
Liang Wei ◽  
Majid Khayatnezhad ◽  
F Faisal
Keyword(s):  
Genetic Diversity ◽  
Conservation Biology ◽  
Issr Markers ◽  
High Genetic Diversity ◽  
Marker Index ◽  
Relationship Analysis ◽  
Inter Simple Sequence Repeats ◽  
Issr Molecular Markers ◽  
Simple Sequence ◽  
Issr Primers

Species identification is fundamentally important within the fields of conservation, biology, biogeography and ecology. Salvia species are herbaceous, biennial or annual, strongly aromatic. Inter-Simple sequence repeats (ISSR) molecular markers were used for evaluate genetic diversity and relationship analysis of 30 Salvia species. Ten selected ISSR primers amplified 116 loci, respectively, of which all were polymorphic. The obtained average polymorphism information content 0.39, average band informativeness 10.5 and the marker index 3.1 revealed high genetic diversity prevailing among Salvia accessions. The dendrogram was constructed based on ISSR separated the individuals into sub-clusters in accordance with their species. Our results indicated that ISSR markers can be used as a reliable and informative technique for evaluation of genetic diversity and relationships among Salvia species. The objectives of present study are: 1) can ISSR markers identify Salvia species, 2) what is the genetic of these taxa in Iran, and 3) to investigate the species inter-relationship?

scholarly journals Study on genetic diversity between Erodium (Geranaiceae) species based on inter-simple sequence repeat markers

Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 927-939
Author(s):  
Shu-Chen Mowang ◽  
Fu-Ji Chen ◽  
Z Zeenat
Keyword(s):  
Biological Activities ◽  
High Capacity ◽  
Molecular Data ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Inter Simple Sequence Repeats ◽  
Whole Plant ◽  
Biological Studies ◽  
Simple Sequence ◽  
Issr Primers

Identifying the accurate boundaries of a species is critical to have a better perspective of any biological studies. Therefore, species delimitation is a subject of extensive part of studies in the framework of biology. Erodium species possess significant pharmacological and biological activities. The whole plant was used as astringent and haemostatic in uterine and other bleeding Therefore, due to the importance of these plant species, we performed a molecular data analysis for this species. For this study, we used 60 randomly collected plants from 5 species in five provinces. Amplification of genomic DNA using 10 primers produced 52 bands, of which 50 were polymorphic (98.48%). The obtained high average PIC and MI values revealed high capacity of ISSR primers to detect polymorphic loci among Erodium species. The genetic similarities of five collections were estimated from 0.77 to 0.91. According to Inter-Simple sequence repeats (ISSR) markers analysis, E. cicutarium and E. malacoides had the lowest similarity and the species of E. malacoides and E. oxyrrhynchum had the highest similarity. The aims of present study are: 1) can ISSR markers identify Erodium species, and 2) to investigate the species inter-relationship? The present study revealed that ISSR markers can identify the species.

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