Dose-Response and Time-Course of a-Tocoferol Mediating the Cytoprotection Of Dental Pulp Cells Against Hydrogen Peroxide

This in vitro study evaluated the potential protective effect of vitamin E alpha-tocopherol (α-T) isomer against the toxicity of hydrogen peroxide (HP) applied on dental pulp cells. Odontoblast-like MDPC-23 cells were seeded on 96-well plates for 72 h, treated with different concentrations of α-T (1, 3, 5, and 10 mM) for different times (1, 4, 8, and 24 h) and then exposed or not to a 0.018% HP solution for 30 min. In positive and negative control groups, cells were exposed to HP or culture medium (DMEM containing 5% DMSO), respectively. Cell viability was assessed by the MTT assay and the absorbance numeric data, expressed as percentage values, were subjected to the statistical analysis by Kruskal-Wallis and Mann-Whitney tests (α=5%). Considering the cells in the negative control as having 100% of cell viability, all combinations of α-T concentrations and pretreatment times showed a protective effect against HP cytotoxicity. Significant reduction of cell viability (59%) was observed in the positive control compared with the negative control. The highest values of pulp cell viability were obtained after pretreatment with 1 and 3 mM α-T concentrations for 24 h followed by exposure to HP (126% and 97% of cell viability, respectively). Under the tested conditions, the most effective cell protection against the cytotoxic effects of HP was provided by the lowest concentrations of α-T (1 and 3 mM) applied for 24 h.

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Protective Effect of Alpha-Tocopherol Isomer from Vitamin E against the H2O2Induced Toxicity on Dental Pulp Cells

BioMed Research International ◽

10.1155/2014/895049 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5 ◽

Cited By ~ 6

Author(s):

Fernanda da Silveira Vargas ◽

Diana Gabriela Soares ◽

Ana Paula Dias Ribeiro ◽

Josimeri Hebling ◽

Carlos Alberto De Souza Costa

Keyword(s):

Vitamin E ◽

Protective Effect ◽

Cell Viability ◽

Dental Pulp ◽

Positive Control ◽

Negative Control ◽

Alpha Tocopherol ◽

Dental Pulp Cells ◽

Cell Protection ◽

Pulp Cells

The aim of this study was to evaluate the protective effects of different concentrations of vitamin E alpha-tocopherol (α-T) isomer against the toxicity of hydrogen peroxide (H2O2) on dental pulp cells. The cells (MDPC-23) were seeded in 96-well plates for 72 hours, followed by treatment with 1, 3, 5, or 10 mMα-T for 60 minutes. They were then exposed or not to H2O2for 30 minutes. In positive and negative control groups, the cells were exposed to culture medium with or without H2O2(0.018%), respectively. Cell viability was evaluated by MTT assay (Kruskal-Wallis and Mann-Whitney tests;α=5%). Significant reduction of cell viability (58.5%) was observed in positive control compared with the negative control. Cells pretreated withα-T at 1, 3, 5, and 10 mM concentrations and exposed to H2O2had their viability decreased by 43%, 32%, 25%, and 27.5%, respectively. These values were significantly lower than those observed in the positive control, thereby showing a protective effect ofα-T against the H2O2toxicity. Overall, the vitamin Eα-T isomer protected the immortalized MDPC-23 pulp cells against the toxic effects of H2O2. The most effective cell protection was provided by 5 and 10 mM concentrations ofα-T.

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Cytotoxicity assessment of different doses of ozonated water on dental pulp cells

BMC Oral Health ◽

10.1186/s12903-021-01392-8 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Ferdiye Küçük ◽

Sibel Yıldırım ◽

Serap Çetiner

Keyword(s):

Cell Viability ◽

Repeated Measures ◽

Dental Pulp ◽

Control Group ◽

Dental Pulp Cells ◽

Time Points ◽

Significant Difference ◽

Pulp Cells ◽

Time Point ◽

Ozonated Water

Abstract Background The purpose of this study was to assess the cytotoxicity of various concentrations of ozonated water (OW) on human primary dental pulp cells. Methods Human primary dental pulp cells were isolated from exfoliated primary canine teeth of an 11-year-old patient with good systemic and oral health. Afterwards, cells were divided into 6 experimental groups; four groups of OW in concentrations of 2 mg/L, 4 mg/L, 8 mg/L, and 16 mg/L, untreated control group, and cell culture without cells. Cytotoxicity was evaluated after exposure for 5-min exposure using Mosmann’s Tetrazolium Toxicity (MTT) assay at 0 h and 48 h time points. Data were analyzed using a repeated measures analysis of variance and Post-hoc tests were performed using Bonferroni correction for multiple comparisons. Results All experimental groups showed proliferation at 0 h time point. However, all groups also experienced a decrease in overtime at 48 h time point (p < 0.05). At both time points 2 mg/L OW showed the highest cell viability as well as proliferation. At 0 h time point, the increase in cell viability for all experimental groups was found statistically significant when compared to positive control group (p < 0.05). At 48 h time point, although 8 mg/L and 16 mg/L OW showed statistically significant reduction in compare to 0 h time point, 2 mg/L and 4 mg/L OW groups didn’t experience any statistically significant difference (p < 0.05). Conclusion Considering our findings, due to ozonated water's induced a higher proliferation rate of dental pulp cells, indicating their biocompatibility and a possible adjuvant on irrigating agent in regenerative endodontic procedures.

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Involvement of SDF-1 and monocyte chemoattractant protein-1 in hydrogen peroxide-induced extracellular matrix degradation in human dental pulp cells

International Endodontic Journal ◽

10.1111/iej.12147 ◽

2013 ◽

Vol 47 (3) ◽

pp. 298-308 ◽

Cited By ~ 9

Author(s):

D.-S. Kim ◽

S. I. Kang ◽

S.-Y. Lee ◽

K.-T. Noh ◽

E.-C. Kim

Keyword(s):

Hydrogen Peroxide ◽

Dental Pulp ◽

Matrix Degradation ◽

Dental Pulp Cells ◽

Monocyte Chemoattractant Protein 1 ◽

Human Dental Pulp Cells ◽

Monocyte Chemoattractant ◽

Monocyte Chemoattractant Protein ◽

Human Dental Pulp ◽

Pulp Cells

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Butein protects human dental pulp cells from hydrogen peroxide-induced oxidative toxicity via Nrf2 pathway-dependent heme oxygenase-1 expressions

Toxicology in Vitro ◽

10.1016/j.tiv.2013.01.003 ◽

2013 ◽

Vol 27 (2) ◽

pp. 874-881 ◽

Cited By ~ 25

Author(s):

Dong-Sung Lee ◽

Bin Li ◽

Kyoung-Su Kim ◽

Gil-Saeng Jeong ◽

Eun-Cheol Kim ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Heme Oxygenase ◽

Dental Pulp ◽

Heme Oxygenase 1 ◽

Dental Pulp Cells ◽

Nrf2 Pathway ◽

Human Dental Pulp Cells ◽

Human Dental Pulp ◽

Pulp Cells

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TOXICITY ANALYSIS OF RGD-CHITOSAN FROM SHRIMP SHELL SCAFFOLD MEMBRANES TOWARD HUMAN DENTAL PULP CELLS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2017.v9s1.07_08 ◽

2017 ◽

Vol 9 ◽

pp. 13

Author(s):

Mauldina Shabrina ◽

Dewi Fatma Suniarti ◽

Lisa R Amir ◽

Erik Idrus

Keyword(s):

Cell Viability ◽

Dental Pulp ◽

Control Group ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

Membrane Toxicity ◽

Toxicity Analysis ◽

Human Dental Pulp ◽

Pulp Cells ◽

Group 2

Objective: This study aimed to analyze RGD-Chitosan from Shrimp Shells’ Scaffolds’ (RCSSS) and CSSS membrane toxicity toward human dental pulpcells.Methods: Human dental pulp cells were cultured for 5 days and then exposed to RCSSS or CSSS membranes for 24 hrs. Cell viability was determinedusing an MTT assay method.Results: Cell viability of the RCSSS group and CSSS group was higher than the cell viability of the control group. The cell viability of the RCSSSgroup 2 mg (537.39%) was significantly higher than the CSSS group 2 mg (301.74%).Conclusions: RCSSS membranes were not toxic toward human dental pulp cells and showed better effect toward human dental pulp cells comparedto CSSS membranes.

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Hydrogen Peroxide Induces Apoptosis in Human Dental Pulp Cells via Caspase-9 Dependent Pathway

Journal of Endodontics ◽

10.1016/j.joen.2013.06.006 ◽

2013 ◽

Vol 39 (9) ◽

pp. 1151-1155 ◽

Cited By ~ 18

Author(s):

Tian-tian Wu ◽

Li-fen Li ◽

Rong Du ◽

Long Jiang ◽

Ya-Qin Zhu

Keyword(s):

Hydrogen Peroxide ◽

Dental Pulp ◽

Dental Pulp Cells ◽

Caspase 9 ◽

Human Dental Pulp Cells ◽

Human Dental Pulp ◽

Pulp Cells ◽

Dependent Pathway

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Solvent and HEMA Increase Adhesive Toxicity and Cytokine Release from Dental Pulp Cells

Materials ◽

10.3390/ma12172750 ◽

2019 ◽

Vol 12 (17) ◽

pp. 2750 ◽

Cited By ~ 4

Author(s):

Helder Massaro ◽

Lígia Zambelli ◽

Auriléia Britto ◽

Rodolfo Vieira ◽

Ana Ligeiro-de-Oliveira ◽

...

Keyword(s):

Cell Viability ◽

Dental Pulp ◽

Culture Medium ◽

Control Group ◽

Dental Pulp Cells ◽

Cytokine Release ◽

Human Dental Pulp Cells ◽

Medium Components ◽

Tnf Α ◽

Pulp Cells

The aim of the present study was to evaluate the effect of the hydroxyethyl-methacrylate (HEMA) concentration and solvent content of dental adhesives on cell viability and cytokine (IL-1b, IL-6, IL-10, TNF-α) release by human dental pulp cells (HDPCs). HDPCs were obtained from fresh extracted human third molars. Experimental adhesives were prepared containing different concentrations of HEMA (0%, 10%, and 20%) with and without solvent (ethanol 10%). Cylindrical specimens were immersed on culture medium during 24 h to obtain the extracts. The cells were incubated with extracts (culture medium + components leached from the adhesives) of different adhesives, and cell viability and cytokine release were evaluated after 6 and 24 h of exposure. Adhesives containing HEMA promoted high cell viability reduction after 6 h of exposure; but after 24 h, the results were similar to the ones found among control group cells. These effects on cell viability were prominently increased with the addition of solvent. Although IL-1b release was not affected by exposure to eluates, other cytokines (IL-10, IL-6, TNF-α) were modulated by the different experiment conditions, directly influenced by the HEMA concentration and presence of solvent. Higher HEMA concentrations, combined with the presence of solvent, can promote significant reduction on HDPC viability, increasing the release of anti- and pro-inflammatory mediators.

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Influence of Hydrogen Peroxide on Mineralization in Dental Pulp Cells: A Systematic Review

Frontiers in Dental Medicine ◽

10.3389/fdmed.2021.689537 ◽

2021 ◽

Vol 2 ◽

Author(s):

Alexandre Henrique dos Reis-Prado ◽

Isadora Rodrigues Grossi ◽

Hebertt Gonzaga dos Santos Chaves ◽

Carolina Bosso André ◽

Luís Fernando dos Santos Alves Morgan ◽

...

Keyword(s):

Systematic Review ◽

Hydrogen Peroxide ◽

Dental Pulp ◽

Cochrane Library ◽

Dental Pulp Cells ◽

Pulp Tissue ◽

Alp Activity ◽

Dental Hard Tissues ◽

Pulp Cells

Background: Dental bleaching agents show the ability to permeate through dental hard tissues, which may lead to pulp tissue changes. This systematic review (PROSPERO register: CRD42020213767) is aimed at understanding the effects of bleaching agents on the process of mineralization of the pulp tissue.Methods: Only in vitro studies evaluating the influence of hydrogen peroxide (HP) on mineralization in dental pulp cells were included. Studies without a non-bleached control group or cells after co-treatment with a bleaching agent other than HP and/or carbamide peroxide were excluded. The primary outcomes evaluated were alkaline phosphatase (ALP) activity and mineralized nodule deposition. The mineralization markers analysis in dental pulp cells and the cell viability were considered secondary outcomes. Two independent authors conducted a systematic search (PubMed/MEDLINE, Scopus, Embase, Cochrane Library, and OpenGrey until January 2021) with no language restrictions and performed data extraction. The quality assessment was appraised according to a modified Joanna Briggs Institute critical appraisal checklist.Results: The search resulted in 473 studies, and 11 were considered eligible. Overall, a reduction in the process of mineralization was observed among pulp cells after bleaching. A reduction in the ALP activity was reported in the mostly bleached groups using different protocols and analysis periods of nine studies. Regarding mineralized nodule deposition, 6 studies reported a significant reduction from 7 to 21 days among bleached groups. Of those three studies that investigated other mineralization markers, two found a reduction in the expression of dentin matrix acidic phosphoprotein (DMP)-1, dentin sialophosphoprotein (DSPP), and matrix extracellular phosphoglycoprotein (MEPE) among some bleaching gel concentrations. In contrast, one study showed a greater expression of osteopontin (OPN) and osteocalcin (OCN) in 100 μmol/L HP after 5 or 10 min of exposure, and another study showed significant induction of DSPP in concentrations of up to 0.5 mmol/L HP.Conclusion: Especially, high concentrations of bleaching gel reduce the potential of mineralization in pulp cells in in vitro studies; however, different HP concentrations, bleaching protocols, and analysis periods can influence this outcome.

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