scholarly journals Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

2019 ◽  
Vol 49 (12) ◽  
Author(s):  
Verucia Maria Dias Brandão ◽  
Pedro Henrique Marques Barrozo ◽  
Luciane Oeiras Sousa ◽  
Rafaelle Cunha dos Santos ◽  
Katiane Schwanke ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Molecular Detection ◽  
Ehrlichia Canis ◽  
Anaplasma Platys ◽  
Blood Samples ◽  
Stray Dogs ◽  
Significant Difference ◽  
The City

ABSTRACT: The occurrence of diseases transmitted by ticks in dogs is very frequent in Brazil, among these diseases we can highlight the ehrlichiosis and anaplasmosis, which are caused by Ehrlichia canis and Anaplasma platys, respectively. The objective of this study was to survey the occurrence of these pathogens in blood samples from domiciled and stray dogs from the city of Belém, Pará. Two hundred and seventy six dogs were sampled for convenience, and the DNA extracted from the blood of these animals was submitted to nested-PCR for research of E. canis and A. platys. E. canis DNA was detected in 39.4% (109/276) and A. platys DNA in 23.1% (64/276) of the samples, there was a statistically significant difference between the frequency of these agents (P<0.0001), and there was coinfection in 13.4% (37/276) of animals. The frequency of detection of these parasites was higher in stray dogs than in those domiciled for both E. canis (OR=2.84) and A. platys (OR=10.5). Considering the results, it was possible to conclude that E. canis and A. platys are present in the studied population, with stray dogs being more affected by these parasites.

scholarly journals Molecular epidemiology of Anaplasma platys, Ehrlichia canis and Babesia vogeli in stray dogs in Paraná, Brazil

2017 ◽  
Vol 37 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Claudia M. Ribeiro ◽  
Aldair C. Matos ◽  
Thainá Azzolini ◽  
Everton R. Bones ◽  
Eduardo A. Wasnieski ◽  
...  
Keyword(s):  
Rhipicephalus Sanguineus ◽  
Molecular Study ◽  
Ehrlichia Canis ◽  
Anaplasma Platys ◽  
Amblyomma Cajennense ◽  
Blood Samples ◽  
Babesia Vogeli ◽  
Polymerase Chain ◽  
Hematological Changes ◽  
The City

ABSTRACT: Hemoparasitic infections are tick-borne diseases, which affect animals and humans. Considering the importance of canine hemoparasitic infections in veterinary clinics, this study aimed to determine the occurrence of Anaplasma platys, Ehrlichia canis and Babesia vogeli in blood samples from 182 dogs not domiciled in the city of Pato Branco, southwestern region of Paraná State, Brazil, using polymerase chain reaction (PCR). The prevalence of A. platys and B. vogeli was 32.9% and 10.9% respectively, and A. platys infection prevailed (p<0.001). The number of dogs positive for A. platys was larger in Winter (p<0.05). All blood samples were negative for E. canis. In the dogs, infestation by Amblyomma cajennense predominated over that by Rhipicephalus sanguineus (p<0.001); but there was no significant association between PCR and the variables presence of ticks, sex and age. Dogs infected by A. platys and B. vogeli showed thrombocytopenia, lymphopenia and leukocytosis; but there was no correlation between such hematological changes and infection by hemoparasites. This appears to be the first molecular study that demonstrates the existence of A. platys and B. vogeli in dogs from the southwestern region of Paraná.

scholarly journals Efeitos de diferentes doses de hiclato de doxiciclina nos parâmetros hematológicos de cães com erliquiose

2016 ◽  
Vol 37 (1) ◽  
pp. 53
Author(s):  
Mariana Cristina Hoeppner Rondelli ◽  
Reinaldo Juan Garrido Palacios Junior ◽  
Igor Luiz Salardani Senhorello ◽  
Áureo Evangelista Santana ◽  
Mirela Tinucci-Costa
Keyword(s):  
Nested Pcr ◽  
Ehrlichia Canis ◽  
Babesia Canis ◽  
Anaplasma Platys ◽  
Leptospira Spp

Resumo: Este estudo objetivou comparar os efeitos de duas doses de hiclato de doxiciclina sobre a contagem de hemácias, concentração de hemoglobina, hematócrito, contagem de leucócitos e plaquetas de cães com erliquiose. Foram estudados os grupos I, composto por cães saudáveis (n=6), negativos na sorologia para Ehrlichia canis e Leptospira spp., na PCR em tempo real para E. canis e Anaplasma platys e na semi–nested PCR para Babesia canis; grupos II (n=6) e III (n=6), compostos por cães com história clínica sugestiva, sorologia e/ou PCR em tempo real positivas para E. canis, e negativos na pesquisa de anticorpos anti-Leptospira spp., na PCR em tempo real para A. platys e na semi–nested PCR para B. canis. Os cães doentes foram tratados com hiclato de doxiciclina via oral a cada 12 horas por 30 dias (5 mg/kg, grupo II; 10 mg/kg, grupo III). Hemogramas foram feitos antes, decorridos 15 dias e aos 10 dias após o término dos tratamentos. Não houve diferenças entre os grupos nos tempos avaliados quanto às variáveis hemácias, hemoglobina, hematócrito e leucócitos. Observou-se diferença quanto aos trombócitos entre o grupo I e os grupos II e III (p<0,0001) no início do estudo. Aos 15 dias de tratamento, a média do grupo III foi menor que dos grupos I (p=0,008) e II (p=0,0007), indicando persistência da trombocitopenia, ausente no grupo II. Não houve diferença entre os grupos no tempo final, sugerindo que ambos os tratamentos promoveram aumento da contagem de plaquetas em cães naturalmente infectados por E. canis.

scholarly journals Occurrence of Ehrlichia canis and Anaplasma platys in household dogs from northern Parana

2012 ◽  
Vol 21 (4) ◽  
pp. 379-385 ◽  
Author(s):  
Gislaine Cristina Ferreira da Silva ◽  
Aline do Nascimento Benitez ◽  
Aline Girotto ◽  
Alessandra Taroda ◽  
Marilda Carlos Vidotto ◽  
...  
Keyword(s):  
Ehrlichia Canis ◽  
Differential Diagnoses ◽  
Laboratory Evaluation ◽  
Blood Collection ◽  
Routine Laboratory ◽  
Anaplasma Platys ◽  
Pcr Assay ◽  
Blood Samples ◽  
Canine Monocytic Ehrlichiosis ◽  
Hematological Alterations

Canine monocytic ehrlichiosis caused primarily by Ehrlichia canis and canine thrombocytic anaplasmosis induced by Anaplasma platys are important emerging zoonotic tick-borne diseases of dogs. There is evidence that these pathogens can also affect humans. This study evaluated the presence of E. canis and A. platys in blood samples collected from 256 domiciled dogs in the municipality of Jataizinho, located in north region of the State of Parana, Brazil, by PCR assay. The occurrence of E. canis and A. platys was 16.4% (42/256) and 19.4% (49/256), respectively; while 5.47% (14/256) of the dogs evaluated were co-infected by these two organisms. The presence of E. canis and A. platys was not significantly associated with the variables evaluated (sex, age, outdoor access, and presence of ticks during blood collection). Infection of dogs by E. canis was associated with anemia and thrombocytopenia, while infection induced by A. platys was related only to thrombocytopenia. Canine monocytic ehrlichiosis and canine thrombocytic anaplasmosis should be included in the differential diagnoses when these hematological alterations are observed during routine laboratory evaluation of dogs.

scholarly journals Molecular detection of feline arthropod-borne pathogens in cats in Cuiabá, state of Mato Grosso, central-western region of Brazil

2013 ◽  
Vol 22 (3) ◽  
pp. 385-390 ◽  
Author(s):  
Natasha Gandolfi Miceli ◽  
Fernando Antonio Gavioli ◽  
Luiz Ricardo Gonçalves ◽  
Marcos Rogério André ◽  
Valéria Régia Franco Sousa ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Western Region ◽  
Mato Grosso ◽  
Animal Shelters ◽  
Blood Samples ◽  
Cytauxzoon Felis ◽  
The City ◽  
Veterinary Hospital

Hemotrophic mycoplasmas (hemoplasmas), Bartonellasp., Hepatozoon sp. and Cytauxzoon felis are prominent pathogens that circulate between cats and invertebrate hosts. The present study aimed to detect the presence of DNA from hemoplasmas,Bartonella sp., Hepatozoon sp. andCytauxzoon felis, and then confirm it by means of sequencing, in blood samples from cats in Cuiabá, MT, Brazil. From February 2009 to February 2011, blood samples with added EDTA were collected from 163 cats that were being housed in four different animal shelters in the city of Cuiabá, state of Mato Grosso, Brazil and from 15 cats that were admitted to the veterinary hospital of the Federal University of Mato Grosso (UFMT). Out of the 178 cats sampled, 15 (8.4%) were positive for hemoplasmas: four (2.2%) forMycoplasma haemofelis, 12 (6.7%) for ‘Candidatus M. haemominutum’ and one (0.5%) for ‘Candidatus M. turicensis’. One cat (0.5%), a patient that was attended at the veterinary hospital, was coinfected with M. haemofelis, ‘Candidatus M. haemominutum’ and ‘Candidatus M. turicensis’, based on sequencing confirmation. Four cats were positive for Bartonella spp.: three (1.7%) for B. henselae and one (0.5%) for B. clarridgeiae. None of the animals showedCytauxzoon sp. or Hepatozoon sp. DNA in their blood samples. This study showed that cats housed in animal shelters in the city of Cuiabá, state of Mato Grosso, are exposed to hemoplasmas andBartonella species.

Molecular detection of Anaplasma platys, Anaplasma phagocytophilum and Wolbachia sp. but not Ehrlichia canis in Croatian dogs

2017 ◽  
Vol 116 (11) ◽  
pp. 3019-3026 ◽  
Author(s):  
Doroteja Huber ◽  
Irena Reil ◽  
Sanja Duvnjak ◽  
Daria Jurković ◽  
Damir Lukačević ◽  
...  
Keyword(s):  
Anaplasma Phagocytophilum ◽  
Molecular Detection ◽  
Ehrlichia Canis ◽  
Anaplasma Platys

scholarly journals Molecular detection of Babesia bovis in cattle in Al-Qadisiyah province

2017 ◽  
Vol 40 (2) ◽  
pp. 155-158
Author(s):  
Noaman N. A,aiz
Keyword(s):  
Polymerase Chain Reaction ◽  
Infection Rate ◽  
Molecular Detection ◽  
Babesia Bovis ◽  
Chain Reaction ◽  
Adult Cattle ◽  
Blood Samples ◽  
Significant Difference ◽  
Polymerase Chain ◽  
Age And Sex

     This study aim to determine Babesia bovis infection in cattle based on genetic methods. A total of 96 blood samples were collected from alive and slaughtered cattle from different areas in addition to the abattoir of Al-Qadisiyah province from December 2013 to August 2014. Real time polymerase chain reaction (RT.PCR) technique was used to detect the presence of the protozoan with the effect of animal's age and sex in the infection rate 47.91 % (46/96) of examined cattle were given positive result to B. bovis infection. The highest infections were shown among the adult cattle (≥1 year), while there was non-significant difference (P>0.05) in the infection rate according to the sex. So the most cattle in Al-Qadisiyah province appear to be bearing the infection predominantly as a carrier hosts.

scholarly journals OCCURRENCE OF TICK-BORNE PATHOGENS IN STRAY DOGS FROM HAVANA, CUBA

2018 ◽  
Vol 3 (4) ◽  
pp. 158-159
Author(s):  
B. G. Corona ◽  
A. A. Díaz-Sánchez ◽  
M. L. Meli ◽  
E. V. Cañizares ◽  
L. R. Arias ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
16S Rrna ◽  
Real Time ◽  
Real Time Pcr ◽  
Ixodid Tick ◽  
Ehrlichia Canis ◽  
Hepatozoon Canis ◽  
Anaplasma Platys ◽  
Rickettsia Felis ◽  
Stray Dogs

The article presents the results of examination of stray dogs from Havana, Cuba for six ixodid tick-borne diseases. Analysis was carried  out using real-time PCR. Overall 107 dogs, 95 (89.09 %) were infected.  41 dogs (38.31 %), 66 (61.68 %), 28 (26.17 %) and 40 (37.38 %)  were found to be infected with Anaplasma platys, Ehrlichia canis,  Rickettsia spp. and Hepatozoon canis, respectively. Sequence analysis of 16S rRNA and groEL genes for Rickettsia spp. revealed 99 % identity  with Rickettsia felis. There were no dogs infected with A. phagocytophilum and Borrelia spp.

scholarly journals INFECÇÃO POR Ehrlichia canis E Anaplasma platys EM CADELAS E NEONATOS DE CUIABÁ, MATO GROSSO

2010 ◽  
Vol 15 (3) ◽  
Author(s):  
Arleana B P F Almeida ◽  
Daphine A J Paula ◽  
Valéria Dutra ◽  
Luciano Nakazato ◽  
Adriane J Mendonça ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Ehrlichia Canis ◽  
Anaplasma Platys ◽  
Mato Grosso

Os canídeos podem ser infectados por diversos agentes pertencentes à Família Anaplasmataceae dentre os mais comumente relatados encontram-se Ehrlichia canis e Anaplasma platys, causando a ehrlichiose monocítica canina e a trombocitopenia infecciosa cíclica canina, respectivamente. O diagnóstico pode ser feito pela detecção de mórulas em esfregaço sanguíneo, sorologicamente, pelo isolamento, com exceção de A. platys e pelos métodos moleculares, como a PCR. O objetivo desta pesquisa foi avaliar a presença de infecção por E. canis e A. platys em cães neonatos e suas respectivas mães. Durante o período de junho a dezembro de 2006, foram avaliados clinicamente 21 fêmeas hígidas e 40 filhotes de suas respectivas ninhadas com idade entre cinco a 29 dias de idade. Para a avaliação da infecção por E. canis, realizaram-se análise sorológica com o ensaio SNAP 3DX, observação de mórulas em esfregaço sanguíneo e nested PCR e para A. platys, a nested PCR. Dos 61 animais avaliados, 36 (59%) apresentaram-se positivos para E. canis e 16 (26,2%) para A. platys.  Infecção isolada por A. platys e co-infecção com E. canis foi observada em cinco (13,8%) e 11 (30,5%) cães, respectivamente, não havendo diferença estatisticamente significativa (P=0,63). Com os dados obtidos pela técnica da nPCR conseguiu-se detectar infecção por E. canis e A. platys em neonatos e suas respectivas mães, levantando a possibilidade de transmissão transplacentária, entretanto estudos mais aprofundados devem ser realizados para se confirmar tal aspecto, principalmente em áreas endêmicas para a doença.

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