scholarly journals INFECÇÃO POR Ehrlichia canis E Anaplasma platys EM CADELAS E NEONATOS DE CUIABÁ, MATO GROSSO

2010 ◽  
Vol 15 (3) ◽  
Author(s):  
Arleana B P F Almeida ◽  
Daphine A J Paula ◽  
Valéria Dutra ◽  
Luciano Nakazato ◽  
Adriane J Mendonça ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Ehrlichia Canis ◽  
Anaplasma Platys ◽  
Mato Grosso

Os canídeos podem ser infectados por diversos agentes pertencentes à Família Anaplasmataceae dentre os mais comumente relatados encontram-se Ehrlichia canis e Anaplasma platys, causando a ehrlichiose monocítica canina e a trombocitopenia infecciosa cíclica canina, respectivamente. O diagnóstico pode ser feito pela detecção de mórulas em esfregaço sanguíneo, sorologicamente, pelo isolamento, com exceção de A. platys e pelos métodos moleculares, como a PCR. O objetivo desta pesquisa foi avaliar a presença de infecção por E. canis e A. platys em cães neonatos e suas respectivas mães. Durante o período de junho a dezembro de 2006, foram avaliados clinicamente 21 fêmeas hígidas e 40 filhotes de suas respectivas ninhadas com idade entre cinco a 29 dias de idade. Para a avaliação da infecção por E. canis, realizaram-se análise sorológica com o ensaio SNAP 3DX, observação de mórulas em esfregaço sanguíneo e nested PCR e para A. platys, a nested PCR. Dos 61 animais avaliados, 36 (59%) apresentaram-se positivos para E. canis e 16 (26,2%) para A. platys.  Infecção isolada por A. platys e co-infecção com E. canis foi observada em cinco (13,8%) e 11 (30,5%) cães, respectivamente, não havendo diferença estatisticamente significativa (P=0,63). Com os dados obtidos pela técnica da nPCR conseguiu-se detectar infecção por E. canis e A. platys em neonatos e suas respectivas mães, levantando a possibilidade de transmissão transplacentária, entretanto estudos mais aprofundados devem ser realizados para se confirmar tal aspecto, principalmente em áreas endêmicas para a doença.

scholarly journals Efeitos de diferentes doses de hiclato de doxiciclina nos parâmetros hematológicos de cães com erliquiose

2016 ◽  
Vol 37 (1) ◽  
pp. 53
Author(s):  
Mariana Cristina Hoeppner Rondelli ◽  
Reinaldo Juan Garrido Palacios Junior ◽  
Igor Luiz Salardani Senhorello ◽  
Áureo Evangelista Santana ◽  
Mirela Tinucci-Costa
Keyword(s):  
Nested Pcr ◽  
Ehrlichia Canis ◽  
Babesia Canis ◽  
Anaplasma Platys ◽  
Leptospira Spp

Resumo: Este estudo objetivou comparar os efeitos de duas doses de hiclato de doxiciclina sobre a contagem de hemácias, concentração de hemoglobina, hematócrito, contagem de leucócitos e plaquetas de cães com erliquiose. Foram estudados os grupos I, composto por cães saudáveis (n=6), negativos na sorologia para Ehrlichia canis e Leptospira spp., na PCR em tempo real para E. canis e Anaplasma platys e na semi–nested PCR para Babesia canis; grupos II (n=6) e III (n=6), compostos por cães com história clínica sugestiva, sorologia e/ou PCR em tempo real positivas para E. canis, e negativos na pesquisa de anticorpos anti-Leptospira spp., na PCR em tempo real para A. platys e na semi–nested PCR para B. canis. Os cães doentes foram tratados com hiclato de doxiciclina via oral a cada 12 horas por 30 dias (5 mg/kg, grupo II; 10 mg/kg, grupo III). Hemogramas foram feitos antes, decorridos 15 dias e aos 10 dias após o término dos tratamentos. Não houve diferenças entre os grupos nos tempos avaliados quanto às variáveis hemácias, hemoglobina, hematócrito e leucócitos. Observou-se diferença quanto aos trombócitos entre o grupo I e os grupos II e III (p<0,0001) no início do estudo. Aos 15 dias de tratamento, a média do grupo III foi menor que dos grupos I (p=0,008) e II (p=0,0007), indicando persistência da trombocitopenia, ausente no grupo II. Não houve diferença entre os grupos no tempo final, sugerindo que ambos os tratamentos promoveram aumento da contagem de plaquetas em cães naturalmente infectados por E. canis.

scholarly journals Ehrlichia canis e Anaplasma platys em carrapatos de cães de Cuiabá, Mato Grosso

2012 ◽  
Vol 33 (3) ◽  
pp. 1123-1126 ◽  
Author(s):  
Arleana do Bom P F de Almeida ◽  
Daphine Ariadne Jesus de Paula ◽  
Magyda Arabia A Dahroug ◽  
Agradia Goncalves de Freitas ◽  
Jose Nivaldo da Silva ◽  
...  
Keyword(s):  
Ehrlichia Canis ◽  
Anaplasma Platys ◽  
Mato Grosso

scholarly journals ComparaÇão de nested-PCR com o diagnóstico direto na detecÇão de Ehrlichia canis e Anaplasma platys em cães

2009 ◽  
Vol 18 (e1) ◽  
pp. 58-62 ◽  
Author(s):  
C. A. N. Ramos ◽  
R. A. N. Ramos ◽  
F. R. Araújo ◽  
D. S. Guedes Júnior. ◽  
I. I. F. Souza ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Ehrlichia Canis ◽  
Anaplasma Platys

scholarly journals Molecular survey of Anaplasma platys and Ehrlichia canis in dogs from Campo Grande, Mato Grosso do Sul, Brazil

2017 ◽  
Vol 89 (1) ◽  
pp. 301-306 ◽  
Author(s):  
RODRIGO SOARES ◽  
CARLOS ALBERTO RAMOS ◽  
THATIANNA PEDROSO ◽  
VERÔNICA BABO-TERRA ◽  
HERBERT CLEVELAND ◽  
...  
Keyword(s):  
Ehrlichia Canis ◽  
Anaplasma Platys ◽  
Mato Grosso ◽  
Molecular Survey ◽  
Mato Grosso Do Sul

scholarly journals Molecular detection of Cercopithifilaria bainae and other tick-borne pathogens in Rhipicephalus sanguineus s.l. isolated from dogs in Midwest Brazil

2020 ◽  
Vol 29 (1) ◽  
Author(s):  
Rodrigo Leite Soares ◽  
Angélica Oliveira da Silva ◽  
Mateus Lotério Coelho ◽  
Jessica Teles Echeverria ◽  
Michelli Lopes de Souza ◽  
...  
Keyword(s):  
Rhipicephalus Sanguineus ◽  
Ehrlichia Canis ◽  
Hepatozoon Canis ◽  
Anaplasma Platys ◽  
16S Rrna Analysis ◽  
Mato Grosso ◽  
Babesia Vogeli ◽  
Cercopithifilaria Bainae ◽  
Definition Of ◽  
Mato Grosso Do Sul

Abstract The aim of the present study was to detect Cercopithifilaria bainae and other tick-borne pathogens and to perform molecular characterization of the tick Rhipicephalus sanguineus s.l. collected from dogs. Ticks (n = 432, including 8 larvae, 59 nymphs, and 365 adults) were sampled from domiciled dogs (n = 73) living in Campo Grande, Mato Grosso do Sul (Midwest Brazil). All ticks were morphologically identified as R. sanguineus. Genomic DNA was extracted in pools (three to five ticks per animal) and was used for definition of R. sanguineus haplotypes (based on 16S rRNA analysis) and pathogen identification (Cercopithifilaria sp., Ehrlichia canis, Anaplasma platys, Hepatozoon canis, Babesia vogeli and Rickettsia spp.). Rhipicephal us sanguineus specimens were identified as haplotypes A and B. DNA of Cercopithifilaria bainae (43.83%; 32/73), Ehrlichia canis (24.65%; 18/73), Anaplasma platys (19.17%; 14/73), and Hepatozoon canis (5.47%; 4/73) was detected. The identity of pathogens was confirmed by DNA sequence analysis. The present study confirms the presence of haplotypes A and B of R. sanguineus in the state of Mato Grosso do Sul and its importance as a vector of several pathogens of veterinary concern. Finally, this is the first report to identify C. bainae in ticks in the Midwestern region of Brazil.

scholarly journals Molecular detection of Ehrlichia canis and Anaplasma platys in dogs from municipality of Belém, State of Pará, Brazil

2019 ◽  
Vol 49 (12) ◽  
Author(s):  
Verucia Maria Dias Brandão ◽  
Pedro Henrique Marques Barrozo ◽  
Luciane Oeiras Sousa ◽  
Rafaelle Cunha dos Santos ◽  
Katiane Schwanke ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Molecular Detection ◽  
Ehrlichia Canis ◽  
Anaplasma Platys ◽  
Blood Samples ◽  
Stray Dogs ◽  
Significant Difference ◽  
The City

ABSTRACT: The occurrence of diseases transmitted by ticks in dogs is very frequent in Brazil, among these diseases we can highlight the ehrlichiosis and anaplasmosis, which are caused by Ehrlichia canis and Anaplasma platys, respectively. The objective of this study was to survey the occurrence of these pathogens in blood samples from domiciled and stray dogs from the city of Belém, Pará. Two hundred and seventy six dogs were sampled for convenience, and the DNA extracted from the blood of these animals was submitted to nested-PCR for research of E. canis and A. platys. E. canis DNA was detected in 39.4% (109/276) and A. platys DNA in 23.1% (64/276) of the samples, there was a statistically significant difference between the frequency of these agents (P<0.0001), and there was coinfection in 13.4% (37/276) of animals. The frequency of detection of these parasites was higher in stray dogs than in those domiciled for both E. canis (OR=2.84) and A. platys (OR=10.5). Considering the results, it was possible to conclude that E. canis and A. platys are present in the studied population, with stray dogs being more affected by these parasites.

scholarly journals Serologic and Molecular Diagnosis of Anaplasma platys and Ehrlichia canis Infection in Dogs in an Endemic Region

Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 488
Author(s):  
Bianca Lara ◽  
Anne Conan ◽  
Mary Anna Thrall ◽  
Jennifer K. Ketzis ◽  
Gillian Carmichael Branford ◽  
...  
Keyword(s):  
Early Stage ◽  
Point Of Care ◽  
False Negative ◽  
Ehrlichia Canis ◽  
Test Results ◽  
Anaplasma Platys ◽  
Endemic Region ◽  
Life Threatening ◽  
Improve Patient Management ◽  
Antibody Levels

Anaplasma platys and Ehrlichia canis are obligate intracellular, tick-borne rickettsial pathogens of dogs that may cause life-threatening diseases. In this study, we assessed the usefulness of PCR and a widely used commercial antibody-based point-of-care (POC) test to diagnose A. platys and E. canis infection and updated the prevalence of these pathogens in dogs inhabiting the Caribbean island of Saint Kitts. We detected A. platys in 62/227 (27%), E. canis in 84/227 (37%), and the presence of both in 43/227 (19%) of the dogs using PCR. POC testing was positive for A. platys in 53/187 (28%), E. canis in 112/187 (60%), and for both in 42/187 (22%) of the samples tested. There was only a slight agreement between A. platys PCR and POC test results and a fair agreement for E. canis PCR and POC test results. Our study suggests that PCR testing may be particularly useful in the early stage of infection when antibody levels are low or undetectable, whereas, POC test is useful when false-negative PCR results occur due to low bacteremia. A combination of PCR and POC tests may increase the ability to diagnose A. platys and E. canis infection and consequently will improve patient management.

scholarly journals Soroprevalência de anticorpos anti-Ehrlichia canis em cães de Cuiabá, Mato Grosso

2010 ◽  
Vol 19 (02) ◽  
pp. 108-111 ◽  
Author(s):  
J. N. da Silva ◽  
A. do B. P. F. de Almeida ◽  
E. da C. Boa Sorte ◽  
A. G. de Freitas ◽  
L. G. F. do Santos ◽  
...  
Keyword(s):  
Ehrlichia Canis ◽  
Mato Grosso

scholarly journals Occurrence of Ehrlichia canis and Anaplasma platys in household dogs from northern Parana

2012 ◽  
Vol 21 (4) ◽  
pp. 379-385 ◽  
Author(s):  
Gislaine Cristina Ferreira da Silva ◽  
Aline do Nascimento Benitez ◽  
Aline Girotto ◽  
Alessandra Taroda ◽  
Marilda Carlos Vidotto ◽  
...  
Keyword(s):  
Ehrlichia Canis ◽  
Differential Diagnoses ◽  
Laboratory Evaluation ◽  
Blood Collection ◽  
Routine Laboratory ◽  
Anaplasma Platys ◽  
Pcr Assay ◽  
Blood Samples ◽  
Canine Monocytic Ehrlichiosis ◽  
Hematological Alterations

Canine monocytic ehrlichiosis caused primarily by Ehrlichia canis and canine thrombocytic anaplasmosis induced by Anaplasma platys are important emerging zoonotic tick-borne diseases of dogs. There is evidence that these pathogens can also affect humans. This study evaluated the presence of E. canis and A. platys in blood samples collected from 256 domiciled dogs in the municipality of Jataizinho, located in north region of the State of Parana, Brazil, by PCR assay. The occurrence of E. canis and A. platys was 16.4% (42/256) and 19.4% (49/256), respectively; while 5.47% (14/256) of the dogs evaluated were co-infected by these two organisms. The presence of E. canis and A. platys was not significantly associated with the variables evaluated (sex, age, outdoor access, and presence of ticks during blood collection). Infection of dogs by E. canis was associated with anemia and thrombocytopenia, while infection induced by A. platys was related only to thrombocytopenia. Canine monocytic ehrlichiosis and canine thrombocytic anaplasmosis should be included in the differential diagnoses when these hematological alterations are observed during routine laboratory evaluation of dogs.

scholarly journals Detection of Pneumocystis in lungs of bats from Brazil by PCR amplification

2009 ◽  
Vol 29 (6) ◽  
pp. 469-473 ◽  
Author(s):  
Edna Maria Cavallini Sanches ◽  
Susi M. Pacheco ◽  
Alison S. Cericatto ◽  
Rosane M. Melo ◽  
Edson Molleta Colodel ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Urban Areas ◽  
Pcr Amplification ◽  
Small Subunit ◽  
Rrna Gene ◽  
Wild Animals ◽  
Tadarida Brasiliensis ◽  
Desmodus Rotundus ◽  
Mato Grosso ◽  
Wide Range

Pneumocystis has been isolated from a wide range of unrelated mammalian hosts, including humans, domestic and wild animals. It has been demonstrated that the genome of Pneumocystis of one host differs markedly from that of other hosts. Also, variation in the chromosome and DNA sequence of Pneumocystis within a single host species has been observed. Since information about the occurrence and nature of infections in wild animals is still limited, the objective of this work was to detect the presence of Pneumocystis sp. in lungs of bats from two states from Brazil by Nested-PCR amplification. The bats, captured in caves and in urban areas, were obtained from the Program of Rabies Control of two States in Brazil, Mato Grosso and Rio Grande do Sul, located in the Mid-Western and Southern regions of the country, respectively. DNAs were extracted from 102 lung tissues and screened for Pneumocystis by nested PCR at the mtLSU rRNA gene and small subunit of mitochondrial ribosomal RNA (mtSSU rRNA). Gene amplification was performed using the mtLSU rRNA, the primer set pAZ102H - pAZ102E and pAZ102X - pAZY, and the mtSSU rRNA primer set pAZ102 10FRI - pAZ102 10R-RI and pAZ102 13RI - pAZ102 14RI. The most frequent bats were Tadarida brasiliensis (25), Desmodus rotundus (20), and Nyctinomops laticaudatus (19). Pneumocystis was more prevalent in the species Nyctinomops laticaudatus (26.3% = 5/19), Tadarida brasiliensis (24% = 6/25), and Desmodus rotundus (20% = 4/20). Besides these species, Pneumocystis also was detected in lungs from Molossus molossus (1/11, 9.1%), Artibeus fimbriatus (1/1, 100%), Sturnira lilium (1/3, 33.3%), Myotis levis (2/3, 66.7%)and Diphylla ecaudata (1/2, 50%). PCR products which could indicate the presence of Pneumocystis (21.56%) were identified in DNA samples obtained from 8 out of 16 classified species from both states (5 bats were not identified). This is the first report of detection of Pneumocystis in bats from Brazil.

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