scholarly journals ITS-2 sequences-based identification of Trichogramma species in South America

2015 ◽  
Vol 75 (4) ◽  
pp. 974-982 ◽  
Author(s):  
R. P. Almeida ◽  
R. Stouthamer
Keyword(s):  
South America ◽  
Species Identification ◽  
Internal Transcribed Spacer ◽  
Restriction Analysis ◽  
Molecular Technique ◽  
Reliable Identification ◽  
Trichogramma Species ◽  
Dichotomous Key ◽  
Pcr Product ◽  
First Time

Abstract ITS2 (Internal transcribed spacer 2) sequences have been used in systematic studies and proved to be useful in providing a reliable identification of Trichogramma species. DNAr sequences ranged in size from 379 to 632 bp. In eleven T. pretiosum lines Wolbachia-induced parthenogenesis was found for the first time. These thelytokous lines were collected in Peru (9), Colombia (1) and USA (1). A dichotomous key for species identification was built based on the size of the ITS2 PCR product and restriction analysis using three endonucleases (EcoRI, MseI and MaeI). This molecular technique was successfully used to distinguish among seventeen native/introduced Trichogramma species collected in South America.

scholarly journals New Baetidae (Insecta: Ephemeroptera) Records from Venezuela and Nymph Description of an UnnamedFallceonSpecies

2012 ◽  
Vol 2012 ◽  
pp. 1-6
Author(s):  
Paulo Vilela Cruz ◽  
Rafael Boldrini ◽  
Carlos F. Quinto ◽  
Hortencia Frontado
Keyword(s):  
South America ◽  
Species Identification ◽  
Number Of Species ◽  
First Time

The Ephemeroptera fauna in Venezuela is poorly known, as is also the case in others areas in South America. Recently, two studies increased from 33 to 50 the number of species recorded in Venezuela. The objective of the present study is to report for the first time in Venezuela the following species:Baetodes levis;Camelobaetidius edmundsi;Fallceonsp.;Mayobaetis ellenae.The nymph ofFallceonsp. is described, but is not named because adults were not reared to allow species identification; however, description of this nymph indicates the presence of this genus in South America.

scholarly journals Podostemaceae in Southern Brazil

Rodriguésia ◽  
2011 ◽  
Vol 62 (4) ◽  
pp. 867-885 ◽  
Author(s):  
Anderson Santos de Mello ◽  
Aldaléa Sprada Tavares ◽  
Rafael Trevisan
Keyword(s):  
South America ◽  
Species Identification ◽  
Equatorial Region ◽  
Southern Brazil ◽  
Taxonomic Treatment ◽  
Dichotomous Key ◽  
Taxonomic Studies ◽  
Aquatic Angiosperms

Abstract This study provides a taxonomic treatment of the Podostemaceae family in southern Brazil. Podostemaceae is the largest family of strictly aquatic angiosperms. The center of family richness is the equatorial region of South America. Taxonomic studies are still scarce in Brazil. For southern Brazil there are six genera and 10 species recognized. Dichotomous key and illustrations are presented for species identification.

Fruit fly (Diptera: Tephritidae) species identification: a rapid molecular diagnostic technique for quarantine application

1997 ◽  
Vol 87 (2) ◽  
pp. 111-118 ◽  
Author(s):  
K. F. Armstrong ◽  
C. M. Cameron ◽  
E. R. Frampton
Keyword(s):  
New Zealand ◽  
Restriction Analysis ◽  
Life Stage ◽  
Geographic Origin ◽  
Diagnostic Technique ◽  
Molecular Diagnostic ◽  
Molecular Technique ◽  
Generic Level ◽  
Restriction Patterns ◽  
Pcr Product

AbstractNew Zealand is currently the only major fruit producing country in the world that is free of economically important fruit flies. As part of the effort to maintain this status, there is a need to supplement quarantine decision-making procedures with a means of rapidly identifying immature life stage infestations to the species level. Here we describe a molecular method that achieves this, using simple restriction patterns of ribosomal DNA (rDNA) as diagnostic markers. The 18S and 18S plus internal transcribed spacer (ITS) regions were amplified from larval DNA by the polymerase chain reaction (PCR). Nineteen species, spanning four genera (including five subgenera ofBactrocera) were analysed. Restriction analysis of the 18S PCR product provided poor resolution, even at the generic level. Digestion of the 18S + ITS PCR product, however, generated thirteen diagnostic haplotypes as defined by the composite restriction patterns fromRsaI,Sau3aHaeIII andAluI. No variation was detected at these restriction sites within or between populations. Twenty two restriction enzymes have been screened, but diagnostic RFLPs have yet to been found for six out of the tenBactrocera (Bactrocera)species;B. passiflorae(Froggatt) cannot be distinguished fromB. facialis(Coquillet), norB. kirki(Froggatt) fromB. trilineola(Froggatt) orB. neohumeralis(Hardy) fromB. tryoni(Froggatt). Geographic origin could assist in distinguishing the first four species, but the latter pair are very closely related with overlapping origins, hosts and adult morphology. All six species, however, are considered high risk with respect to their likely establishment in New Zealand. Therefore diagnosis based on this molecular technique would support the same quarantine decision. We consider this method could be useful as a diagnostic technique and discuss directions for further development.

Effects of insecticides on adults and eggs of Drosophila suzukii (Diptera, Drosophilidae)

2017 ◽  
Vol 43 (2) ◽  
pp. 208 ◽  
Author(s):  
Daniele Cristine Hoffmann Schlesener ◽  
Jutiane Wollmann ◽  
Juliano De Bastos Pazini ◽  
Anderson Dionei Grützmacher ◽  
Flávio Roberto Mello Garcia
Keyword(s):  
North America ◽  
South America ◽  
Chemical Control ◽  
Stone Fruits ◽  
Drosophila Suzukii ◽  
Ovicidal Effect ◽  
Mere Presence ◽  
South Of Brazil ◽  
Different Cultures ◽  
First Time

Drosophila suzukii (Diptera, Drosophilidae) is an exotic species, endemic to Asia and currently a pest to small and stone fruits in several countries of North America and Europe. It was detected in 2013 for the first time in South America, in the south of Brazil. Unlike most drosophilids, this species deserves special attention, because the females are capable of oviposit inside healthy fruits, rendering their sale and export prohibited. Despite the confirmed existence of this species in different states of Brazil, this insect is yet been to be given the pest status. Nevertheless, the mere presence of this species is enough to cause concern to producers of small fruits and to justify further investigation for it’s control, especially chemical control for a possible change in status. Therefore, the goal of this work was to evaluate, in laboratory, mortality of D. suzukii adults and ovicidal effect when exposed to different insecticides registered for species of the Tephritidae and Agromyzidae families in different cultures. The insecticides deltamethrin, dimethoate, spinosad, fenitrothion, phosmet, malathion, methidathion, and zeta-cypermethrin resulted in mortality to 100 % of the subjects three days after the treatment (DAT). Regarding the effects over eggs, it was  established that the insecticides fenitrothion, malathion, and methidathion deemed 100 % of the eggs not viable, followed by phosmet and diflubenzuron, which also caused elevated reduction in the eclosion of larvae two DAT.

Cas de suie de l'érable et de chancre du peuplier dans le canton de Genève

2016 ◽  
Vol 167 (2) ◽  
pp. 98-104
Author(s):  
Bastien Cochard ◽  
François Lefort
Keyword(s):  
Internal Transcribed Spacer ◽  
Pathogenic Fungi ◽  
Fungal Flora ◽  
Acer Campestre ◽  
Cytospora Chrysosperma ◽  
Pure Cultures ◽  
Populus X Euramericana ◽  
Bark Peeling ◽  
Specific Symptoms ◽  
First Time

A case of sooty bark disease and Cytospora poplar canker in the Canton of Geneva In summer 2014, a case of sooty bark disease caused by Cryptostroma corticale on an individual field maple (Acer campestre) and two cases of poplar canker due to Cytospora chrysosperma on Populus x euramericana were identified genetically for the first time on the territory of the Canton of Geneva. In both cases, the trees presented signs of very advanced dieback, accompanied by specific symptoms such as bark peeling and sooty plaques for the maple, and loose twisted bark layers and black colouring of the wood in structural branches of the poplars. Sampling was carried out in the symptomatic areas and components of the fungal flora were isolated in pure cultures in order to identify any pathogenic fungi. The molecular analysis of the rDNA internal transcribed spacer (ITS) sequences made it possible to identify precisely all pure isolates obtained. The results showed a majority presence of C. corticale in the maple tree, and of C. chrysosperma in the two poplars. Both these fungi are little known in Switzerland and Europe, and their presence is maybe associated with changes in the climate.

Additions to the Placopsis mycobiota (Trapeliaceae, Ascomycota) of southern South America, with notes on new records (including Aspiciliopsis macrophthalma), and a revised regional key to the species

2010 ◽  
Vol 42 (6) ◽  
pp. 727-737 ◽  
Author(s):  
David J. GALLOWAY
Keyword(s):  
South America ◽  
New Records ◽  
Southern South America ◽  
First Time

AbstractAspiciliopsis macrophthalma, Placopsis fusciduloides, P. gelidioides and P. tararuana are reported for the first time from southern South America. New records for 13 species of Placopsis in southern South America are reported, and a revised key to 22 species of Placopsis and A. macrophthalma in the region is given.

scholarly journals First Report of Echinococcus ortleppi in Free-Living Wild Boar (Sus scrofa) from Portugal

2021 ◽  
Vol 9 (6) ◽  
pp. 1256
Author(s):  
Teresa Letra Mateus ◽  
Maria João Gargaté ◽  
Anabela Vilares ◽  
Idalina Ferreira ◽  
Manuela Rodrigues ◽  
...  
Keyword(s):  
Wild Boar ◽  
Internal Transcribed Spacer ◽  
Sus Scrofa ◽  
One Health ◽  
Control Strategies ◽  
Free Living ◽  
First Report ◽  
Wildlife Reservoirs ◽  
First Time ◽  
Echinococcus Granulosus Sensu Lato

Cystic echinococcosis (CE) is a zoonosis that is prevalent worldwide. It is considered endemic in Portugal but few studies have been performed on Echinococcus granulosus sensu lato and their hosts. In this study, CE cysts are reported for the first time in a free-living wild boar (Sus scrofa) in Portugal. The presence of the metacestodes in the liver of the wild boar was identified by morphological features, microscopic examination and molecular analysis. The sequencing of part of the DNA nuclear ribosomal internal transcribed spacer-1 (ITS-1) region revealed a G5 genotype that presently corresponds to Echinococcus ortleppi. This is the first report of E. ortleppi in Portugal and to the best of the authors’ knowledge, in Europe. These results suggest that wild boar may be a host of CE, namely, crossing the livestock–wildlife interface, which has important public health implications. Wildlife reservoirs must be taken into account as CE hosts and surveillance of game as well as health education for hunters should be implemented using a One Health approach, with implementation of feasible and tailor-made control strategies, namely, proper elimination of byproducts in the field.

scholarly journals Prevalence of Eimeria spp. in calves from dairy farms in northern Paraná state, Brazil

2018 ◽  
Vol 27 (1) ◽  
pp. 118-122
Author(s):  
Sérgio Tosi Cardim ◽  
Mércia Seixas ◽  
Victor Bittencourt Dutra Tabacow ◽  
Alessandra Taroda ◽  
Priscilla Gomes Carneiro ◽  
...  
Keyword(s):  
Species Identification ◽  
Dairy Farms ◽  
Northern Region ◽  
The State ◽  
Pathogenic Species ◽  
Eimeria Spp ◽  
High Prevalence ◽  
The World ◽  
Cattle Herds ◽  
First Time

Abstract Bovine coccidiosis is a disease of major importance in cattle herds across the world. The disorder mainly affects young calves, and E. bovis and E. zuernii are considered the most pathogenic species of the genus, however, E. alabamensis have been described in grazing calves. In this study, the prevalence of Eimeria spp. was evaluated in calves on dairy farms in the northern region of the state of Paraná, Brazil. Four hundred calves on 44 dairy farms were tested for the presence of coccidian oocysts. The positives were re-examined and the oocysts were morphometrically analyzed for species identification. All the farms were contaminated and 205 animals (51.25%) presented Eimeria spp. oocysts. Among these, 146 animals (71.22%) were co-infected by two or more species of coccidia. Ten species of Eimeria were identified: E. bovis (in 30.25% of the positive samples), E. alabamensis (26.75%), E. zuernii (22.00%), E. ellipsoidalis (18.50%), E. auburnensis (13.75%), E. canadensis (8.00%), E. cylindrica (7.25%), E. subspherica (5.00%), E. bukidnonensis (3.00%) and E. brasiliensis (0.75%). This study demonstrates the high prevalence of Eimeria spp. in the northern region of Paraná, Brazil, and detection for the first time in our region the pathogenic species E. alabamensis.

A review of Sharp's types of Aleochara from Latin America (Coleoptera: Staphylinidae)

1989 ◽  
Vol 20 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Jan Klimaszewski
Keyword(s):  
Latin America ◽  
South America ◽  
North American ◽  
Additional Information ◽  
First Time

AbstractA review of David Sharp's types of Aleochara from Mexico, and Central and South America is presented. Nineteen species are treated here, one of which is described as new (A. felixiana), seven are redescribed [A. angusticeps Sharp, A. funestior Sharp, A. mexicana Sharp, A. oxypodia Sharp, A. simulatrix Sharp, A. gracilis (Sharp), and A. duplicata (Sharp)] and one [A. miradoris Sharp] is put in synonymy under A. mexicana Sharp. The remaining ten species were treated in my 1984-revision of North American Aleochara but are here included in a checklist with references to description, redescription, and illustrations. Some additional information is provided for previously synonymized A. torquata Sharp (= A. sallaei Sharp), and for A. quadrata Sharp. Illustrations of genitalic structures of both sexes are provided for the first time for the seven redescribed species. Lectotypes are designated for A. angusticeps, A. duplicata, A. funestior, A. mexicana, and A. oxypodia.

Pomacea maculata.

2021 ◽  
Author(s):  
Rob Cowie ◽  
Romi L. Burks ◽  
Amy E. Miller ◽  
Alexandria L. Hill
Keyword(s):  
Invasive Species ◽  
South America ◽  
Species Identification ◽  
Geographical Area ◽  
Freshwater Snail ◽  
Pomacea Canaliculata ◽  
Apple Snail ◽  
Pomacea Maculata ◽  
Golden Apple Snail ◽  
History Of

Abstract P. maculata is a freshwater snail native to a wide geographical area in South America from the Rio de la Plata in Argentina and Uruguay to the Amazon in Brazil. It is commonly confused with any number of similar large apple snails, including the well-known invasive golden apple snail Pomacea canaliculata (listed among '100 of the world's worst invasive species'). Both species have been introduced to South-East and East Asia, although for many years they were not distinguished and the Asian introductions were widely identified as "golden apple snails" and the name P. canaliculata was applied to them. Due to the confusion in species identification, the history of introduction of P. maculata remains somewhat uncertain as does its invasiveness and pest potential. Much of the literature is confounded, for example, the snails illustrated by Cowie (2002) as P. canaliculata are in fact P. maculata. The majority of invasive populations in Asia appear to be P. canaliculata, often not mixed with P. maculata (Hayes et al., 2008; Tran et al., 2008) and the pest potential of P. canaliculata in such cases is clear. However, much less has been written about the invasiveness and pest potential of 'P. maculata'.

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