PENGARUH SUBSTITUSI DAGING KEONG MAS (Pomacea canaliculata L) DAN DAGING AYAM TERHADAP KARAKTERISTIK ORGANOLEPTIK DAN NILAI GIZI NUGGET

ABSTRACTThis study aimed to determine the effect of golden apple snail and chicken meat substitution on the organoleptic characteristics and nutritional values of chicken nuggets. This study used a single factor completely randomized design (CRD), with five levels of treatment, namely P0 (0% golden apple snail meat: 50% chicken meat: 50% wheat flour), P1 (45% golden apple snail meat: 5% chicken meat: 50% flour), P2 (40% golden apple snail meat: 10% chicken meat: 50% flour), P3 (35% golden apple snail meat: 15% chicken meat: 50% flour), and P4 (30% golden apple snail meat: 52% chicken meat: 50% flour). Data were analyzed using Analysis of Variance (ANOVA). The results show that the substitution treatment of golden apple snail (Pomacea canaliculata l.) and chicken meat had a very significant effect on increasing color, taste, aroma, and texture. The P1 treatment (45% golden apple snail meat; 5% chicken meat and 50% wheat flour) was the most preferred treatment by panelists with preference scores of color, aroma, texture, and taste reached 3.74 (like), 3.93 (like), 3.68 (like), and 3.68 (like), respectively. Meanwhile, the analysis of the nutritional values shows that the selected treatment contained 30.68% water, 2.44% ash, 10.24% fat, 9.04% protein, and 47.6% carbohydrates. Based on the standard of SNI 01-6638-2002, the golden apple snail and chicken meat nuggets met the quality standards on water, ash, and protein contents.Keywords: golden apple snail meat, chicken meat, nuggets.ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh subtitusi keong mas dan daging ayam terhadap karakteristik organoleptik dan nilai gizi pada pembuatan nugget. Penelitian ini menggunakan Rancangan Acak lengkap (RAL) faktor tunggal, dengan lima Perlakuan P0 (daging keong mas 0% : daging ayam 50% : tepung terigu 50%), P1 (daging keong mas 45% : daging ayam 5% : tepung terigu 50%), P2 (daging keong mas 40% : daging ayam 10% : tepung terigu 50%), P3 (daging keong mas 35% : daging ayam 15% : tepung terigu 50%) dan P4 (daging keong mas 30% : daging ayam 52% : tepung terigu 50%). Data dianalisis menggunakan Analysis of Varian (ANOVA). Hasil penelitian menunjukkan bahwa perlakuan Substitusi daging keong mas (pomacea canaliculata l.) dan daging ayam berpengaruh sangat nyata terhadap peningkatan warna, rasa, aroma dan tekstur.. Perlakuan P1 (daging keong mas 45%; daging ayam 5% dan tepung terigu 50%) merupakan perlakuan yang paling disukai panelis dengan skor penilaian kesukaan terhadap warna, aroma, tekstur dan rasa berturut-turut sebesar 3,74 (suka), 3,93 (suka), 3,68 (suka), 3,68 (suka), sedangkan berdasarkan analisis nilai gizi meliputi kadar air, abu, lemak, protein dan karbohidrat berturut-turut sebesar 30,68%, 2,44%, 10,24%, 9,04% dan 47,6%. Berdasarkan standar mutu SNI 01-6638-2002 produk nugget daging keong mas dan daging ayam telah memenuhi standar mutu pada kadar air, kadar abu, dan kadar protein. Sedangkan kadar lemak dan kadar karbohidrat belum memenuhi standar mutu SNI 01-6638-2002Kata kunci: daging keong mas, daging ayam, nugget.

THE MOLLUSCICIDAL EFFECT OF THE STEM EXTRACTS OF Tinospora crispa IN CONTROLLING THE GOLDEN APPLE SNAIL Pomacea canaliculata

This study investigated the molluscicidal effect of the stem extracts of Tinospora crispa in controlling the golden apple snail Pomacea canaliculata. Extracts were prepared in four solvents, i.e., hexane, chloroform, methanol, and distilled water at three concentrations (1,000, 5,000, and 10,000 ppm) per solvent. The phytochemical contents of the extracts were qualitatively identified, and the lethal concentration (LC50) of the extracts for mollusicicidal potential was determined using the probit analysis. The effect of T. crispa extracts on the snail was monitored for three days and the snail mortality was recorded every 24 h. The stem extract prepared in methanol at 10,000 ppm showed the highest molluscicidal effect with a mortality of 80% at 72 h. Phytochemicals identified in the stem extracts included alkaloids, flavonoids, saponin, tannin, and terpenoids. Based on the probit analysis, stem extracts of T. crispa prepared in methanol showed the lowest LC50 value of 3,428 ppm for mollusicicidal potential and followed by extracts prepared in chloroform, hexane, and distilled water at 5,888, 14,771, and 14,993 ppm, respectively.

Saponin Stabilization via Progressive Freeze Concentration and Sterilization Treatment

Saponin is a biopesticide used to suppress the growth of the golden apple snail population. This study aims to determine the stabilized conditions for saponin storage. The maceration process was used for saponin extraction, and for saponin concentration, progressive freeze concentration (PFC) was used. Afterwards, stability analysis was performed by storing the sample for 21 days in two conditions: Room temperature (26 °C) and cold room (10 °C). The samples kept in a cold room were sterilized samples that undergo thermal treatment by placing the sample in the water bath. The non-sterilized samples were kept in room temperature condition for 21 days. The results showed that saponin stored in the cold room (sterilized sample) has low degradation with higher concentration than those stored at room temperature in stability analysis with the highest saponin concentration (0.730 mg/mL) at a concentration temperature of −6 °C and concentration time of 15 min. The lowest saponin concentration obtained by saponin stored at room temperature (non-sterilized sample) is 0.025 mg/mL at a concentration temperature of −6 °C and concentration time of 10 min. Thus, the finding concluded that saponin is sensitive to temperature. Hence, the best storage condition to store saponin after thermal treatment is to keep it in a cold room at 10 °C.

MOLLUSCICIDAL ACTIVITY OF ZINGIBER OFFICINALE AND CARICA PAPAYA ON THE EGG HATCHABILITY OF THE GOLDEN APPLE SNAIL, POMACEA CANALICULATA (GASTROPODA: AMPULIRIIDAE)

The study was conducted to assess the effect of Zingiber officinale and Carica papaya extract on the egg hatchability of the golden apple snail, Pomacea canaliculata. This is a freshwater snail that is considered a pest in agriculture as well as natural ecosystems. In combating the population of the aforementioned pest, this study evaluates the use of plant extracts such as biomolluscicides to control the production of new hatchlings from the clusters of eggs. The egg clusters were exposed to different concentrations of Z. officinale ethanolic extract and C. papaya methanolic extracts (25 ppm, 50 ppm, 100 ppm) using the direct single spraying method. Observation was made for a maximum of 30 days. The time taken for the eggs to hatch and the number of eggs successfully hatched were measured to reflect the potential of both extracts in affecting egg hatchability. The finding shows that the eggs hatch later when exposed to increasing concentrations (25 to 100 ppm) of Z. officinale extract. The number of eggs successfully hatched was significantly decreased from 86% to 9% after exposure to 25 ppm and above. Meanwhile, the extract of C. papaya did not show any significant effect on both parameters for egg hatchability of P. canaliculata, regardless of the increase in concentration from 25 to 100 ppm. Our findings reveal that the extract of Z. officinale contains higher molluscicidal activity than the extract of C. papaya. Therefore, it has the potential to be commercialized as a biomolluscicide to control the population of golden apple snails specifically at the egg stage.

Effect of Shrimp Feed Supplemented with Astaxanthin on Golden Apple Snail Pomacea diffusa

Popularmente conhecidas como ampulárias, esses moluscos gastrópodes são usualmente cultivados como organismos ornamentais pelo mundo. A coloração desses animais ornamentais é um dos pontos que atrai os seus admiradores e estimula sua aquisição no mercado. O pigmento mais difundido como suplemento para intensificação de cor em organismos aquáticos é a astaxantina. Ainda não existem rações específicas para a ampulária reforçando a necessidade de testar alternativas de alimentos e suplementos que melhorem o desempenho produtivo e a atratividade do animal. Os objetivos desse estudo foram mensurar a capacidade de acumulação de astaxantina sintética em diferentes níveis de inclusão na dieta de Pomacea diffusa, sua influência na concentração do pigmento e nos índices zootécnicos ao final do cultivo. E em paralelo verificar a influência da mudança da dieta de ração de peixes para ração de camarão na condição corporal dos moluscos em comparação com o início do trabalho. As ampulárias alimentadas com ração comercial extrusada para camarão marinho tiveram baixos índices de conversão alimentar (126,7 ± 18,3), altos índices de eficiência alimentar (126,7 ± 18,3%) e fator de condição (1,0 ± 0,14) acima da média inicial (0,90 ± 0,17), o que demonstra que esse alimento pode ser utilizado para seu cultivo. A suplementação dietética de astaxantina sintética não resulta em aumento significativo da concentração desse pigmento na musculatura desse molusco (P˃0,05). Palavras-chave: Ampulária. Moluscos. Gastrópodes. Pigmentos. Carotenoides. Abstract Known as apple snail, these gastropods are popular as aquarium pets around the world. The bright color of these animals is one of the key points that attract their attention in the ornamental aquaculture, where deep-colored animals are well appreciated. Astaxanthin is the most widespread color enhancing pigment used as a food supplement for aquatic organisms. There is no specific commercial food for apple snails, emphasizing the need to search for food alternatives and supplements that improve their performance and attractiveness. The objectives of this study are to measure the synthetic astaxanthin accumulation capacity of Pomacea diffusa at different levels of diet supplementation, and the effect on pigment concentration and zootechnical indexes at the end of the culture. It was also aimed to verify the effect of the diet change from fish food to extruded shrimp food on the mollusks’ body condition. Apple snails fed with commercial shrimp ration had low feed conversion rates (0.81 ± 0.13), high food efficiency (126.7 ± 18.3%), and condition factor (1.0 ± 0.14) above the initial average (0.90 ± 0.17), demonstrating that this might be an advantageous alternative food. Dietary supplementation with synthetic astaxanthin did not result in a significant concentration increase of this pigment in muscle tissue of these mollusks (P˃0.05). Keywords: Aquaculture. Mollusks. Gastropods. Pigments. Carotenoids

Pomacea canaliculata (golden apple snail).

P. canaliculata is a freshwater snail native to parts of Argentina and Uruguay. The distribution of P. canaliculata has been steadily increasing since its introduction to Asia, primarily as a human food resource but perhaps also by the aquarium trade, beginning around 1979 or 1980 (Mochida, 1991; Halwart, 1994a; Cowie, 2002; Joshi and Sebastian, 2006). Once introduced to an area, it spreads rapidly through bodies of water such as canals and rivers and during floods. It feeds on aquatic plants and can devastate rice (in South-east Asia), taro (in Hawaii) and other aquatic or semi-aquatic crops. It may out-compete native apple snails (Halwart, 1994a; Warren, 1997), prey on native fauna (Wood et al., 2005, 2006) and alter natural ecosystem function (Carlsson et al. 2004a). It is also an important vector of various parasites including the nematode Angiostrongyulus cantonensis, which causes human eosinophillic meningitis (Lv et al., 2011; Yang et al., 2013).It is listed among '100 of the world's worst invasive species' (Lowe et al., 2000). In the United States its transport between states is restricted (Gaston, 2006), as is its transport between islands in the Hawaiian archipelago (Tamaru et al., 2006). It is listed as a quarantine pest in Malaysia (Yahaya et al., 2006) and in Japan. Australia has strong quarantine restrictions and is particularly concerned about P. canaliculata (Cowie, 2005).

Pomacea maculata.

P. maculata is a freshwater snail native to a wide geographical area in South America from the Rio de la Plata in Argentina and Uruguay to the Amazon in Brazil. It is commonly confused with any number of similar large apple snails, including the well-known invasive golden apple snail Pomacea canaliculata (listed among '100 of the world's worst invasive species'). Both species have been introduced to South-East and East Asia, although for many years they were not distinguished and the Asian introductions were widely identified as "golden apple snails" and the name P. canaliculata was applied to them. Due to the confusion in species identification, the history of introduction of P. maculata remains somewhat uncertain as does its invasiveness and pest potential. Much of the literature is confounded, for example, the snails illustrated by Cowie (2002) as P. canaliculata are in fact P. maculata. The majority of invasive populations in Asia appear to be P. canaliculata, often not mixed with P. maculata (Hayes et al., 2008; Tran et al., 2008) and the pest potential of P. canaliculata in such cases is clear. However, much less has been written about the invasiveness and pest potential of 'P. maculata'.