scholarly journals Evaluation of the Chilli veinal mottle virus CP gene expressing transgenic Nicotiana benthamiana plants for disease resistance against the virus

2022 ◽  
Vol 82 ◽  
Author(s):  
T. Riaz ◽  
M. Ashfaq ◽  
Z. Khan
Keyword(s):  
Nicotiana Benthamiana ◽  
Yield Loss ◽  
Molecular Level ◽  
Plant Viruses ◽  
Mottle Virus ◽  
Cp Gene ◽  
Capsicum Spp ◽  
Pathogen Derived Resistance ◽  
Gene Expressing ◽  
Chilli Veinal Mottle Virus

Abstract Vegetables are an important source of income and high-value crops for small farmers. Chilli (Capsicum spp.) is one of the most economically important vegetables of Pakistan and it is grown throughout the country. It is a rich source of nutrition especially vitamins A, B, C and E along with minerals as folic acid, manganese (Mn), potassium (K) and molybdenum (Mo). Chilli possesses seven times more amount of vitamin C than an orange. Vitamin A, C and beta-carotenoids are strong antioxidants to scavenge the free radicals. Chilli production is restricted due to various biotic factors. Among these viruses, Chilli veinal mottle virus (ChiVMV) is one of the most destructive and menacing agents that inflicts heavy and colossal losses that accounted for 50% yield loss both in quality and quantity. Pathogen-Derived Resistance (PDR) approach is considered one of the effective approaches to manage plant viruses. In this study, ChiVMV was characterized on a molecular level, the coat protein (CP) gene of the virus was stably transformed into Nicotiana benthamiana plants using Agrobacterium tumefaciens. The transgenic plants were challenged with the virus to evaluate the level of resistance of plants against the virus. It was observed that the plants expressing CP gene have partial resistance against the virus in terms of symptoms’ development and virus accumulation. Translation of this technique into elite chilli varieties will be resulted to mitigate the ChiVMV in the crop as well as an economic benefit to the farmers.

scholarly journals KETAHANAN BEBERAPA KULTIVAR CABAI TERHADAP CUCUMBER MOSAIC VIRUS DAN CHILLI VEINAL MOTTLE VIRUS

2012 ◽  
Vol 7 (2) ◽  
pp. 130-139
Author(s):  
Muhammad Taufik ◽  
Sri Hendrastuti Hidayat ◽  
Sriani Sujiprihati ◽  
Gede Suastika ◽  
Sientje Mandang Sumaraw
Keyword(s):  
Virus Infection ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Yield Loss ◽  
Disease Incidence ◽  
Growth And Yield ◽  
Mottle Virus ◽  
Tolerance Response ◽  
Chilli Veinal Mottle Virus ◽  
Das Elisa

Resistance Evaluation of Chillipepper Cultivars for Cucumber Mosaic Virus and Chilli Veinal Mottle Virus.  The use of resistance culivars is an important strategy for management of virus infection in chillipepper. A research was undergone to study the effect of single and mix infection of CMV and ChiVMV on the disease incidence and on the growth and yield of nine chillipepper cultivars, i.e. Cilibangi 4, Cilibangi 5, Cilibangi 6, Helem, Jatilaba, Tit Bulat, Tit Segitiga, Tit Super and Tampar. Mechanical inoculation was conducted to transmit the virus. Infection of the virus was then confirmed with DAS-ELISA.  In general, inoculated chillipepper cultivars developed similar symptoms, i.e. mosaic type for CMV and mottle type for ChiVMV.  More severe symptom was not always observed from mix infection of CMV and ChiVMV. Disease incidence occurred in the range of 16.67 – 86.0% and this caused 18.3 – 98.6% yield loss.  Based on symptom expression, ELISA result, and reduction on yield, it can be concluded that all chillipepper cultivars used in this study could not hold up the virus infection. However, several cultivars showed tolerance response :  Jatilaba, Tit Super, and Tampar for CMV; Cilibangi 4 for ChiVMV; Tit Super for mix infection; and Cilibangi 5 for CMV, ChiVMV, and mix infection.  Further evaluation and investigation involving different chillipepper cultivars should be conducted.

scholarly journals Molecular characterization of the CP gene and 3'UTR of Chilli veinal mottle virus from South and Southeast Asia

2008 ◽  
Vol 57 (3) ◽  
pp. 408-416 ◽  
Author(s):  
W. S. Tsai ◽  
Y. C. Huang ◽  
D. Y. Zhang ◽  
K. Reddy ◽  
S. H. Hidayat ◽  
...  
Keyword(s):  
Southeast Asia ◽  
Molecular Characterization ◽  
Mottle Virus ◽  
South And Southeast Asia ◽  
Cp Gene ◽  
Chilli Veinal Mottle Virus

scholarly journals Towards plant resistance to viruses using protein-only RNase P

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Anthony Gobert ◽  
Yifat Quan ◽  
Mathilde Arrivé ◽  
Florent Waltz ◽  
Nathalie Da Silva ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Replication ◽  
Yield Loss ◽  
Rnase P ◽  
Plant Viruses ◽  
Yellow Mosaic Virus ◽  
Resistance To Viruses ◽  
Target Plant

AbstractPlant viruses cause massive crop yield loss worldwide. Most plant viruses are RNA viruses, many of which contain a functional tRNA-like structure. RNase P has the enzymatic activity to catalyze the 5′ maturation of precursor tRNAs. It is also able to cleave tRNA-like structures. However, RNase P enzymes only accumulate in the nucleus, mitochondria, and chloroplasts rather than cytosol where virus replication takes place. Here, we report a biotechnology strategy based on the re-localization of plant protein-only RNase P to the cytosol (CytoRP) to target plant viruses tRNA-like structures and thus hamper virus replication. We demonstrate the cytosol localization of protein-only RNase P in Arabidopsis protoplasts. In addition, we provide in vitro evidences for CytoRP to cleave turnip yellow mosaic virus and oilseed rape mosaic virus. However, we observe varied in vivo results. The possible reasons have been discussed. Overall, the results provided here show the potential of using CytoRP for combating some plant viral diseases.

scholarly journals First report of Chilli veinal mottle virus in Naga chilli (Capsicum chinense) in Meghalaya, India

VirusDisease ◽  
2013 ◽  
Vol 25 (1) ◽  
pp. 142-143 ◽  
Author(s):  
Amrita Banerjee ◽  
Ram Dutta ◽  
Somnath Roy ◽  
S. V. Ngachan
Keyword(s):  
Mottle Virus ◽  
Capsicum Chinense ◽  
First Report ◽  
Chilli Veinal Mottle Virus

scholarly journals Chloroplast Hsp70 Isoform Is Required for Age-Dependent Tissue Preference of Bamboo mosaic virus in Mature Nicotiana benthamiana Leaves

2017 ◽  
Vol 30 (8) ◽  
pp. 631-645 ◽  
Author(s):  
Ying Wen Huang ◽  
Chung Chi Hu ◽  
Ching Hsiu Tsai ◽  
Na Sheng Lin ◽  
Yau Heiu Hsu
Keyword(s):  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Transit Peptide ◽  
Plant Viruses ◽  
Age Dependent ◽  
Efficient Replication ◽  
Underlying Mechanisms ◽  
Lines Of Evidence ◽  
Suitable Environment

Plant viruses may exhibit age-dependent tissue preference in their hosts but the underlying mechanisms are not well understood. In this study, we provide several lines of evidence to reveal the determining role of a protein of the Nicotiana benthamiana chloroplast Hsp70 (NbcpHsp70) family, NbcpHsp70-2, involved in the preference of Bamboo mosaic virus (BaMV) to infect older tissues. NbcpHsp70 family proteins were identified in complexes pulled down with BaMV replicase as the bait. Among the isoforms of NbcpHsp70, only the specific silencing of NbcpHsp70-2 resulted in the significant decrease of BaMV RNA in N. benthamiana protopalsts, indicating that NbcpHsp70-2 is involved in the efficient replication of BaMV RNA. We further identified the age-dependent import regulation signal contained in the transit peptide of NbcpHsp70-2. Deletion, overexpression, and substitution experiments revealed that the signal in the transit peptide of NbcpHsp70-2 is crucial for both the import of NbcpHsp70-2 into older chloroplasts and the preference of BaMV for infecting older leaves of N. benthamiana. Together, these data demonstrated that BaMV may exploit a cellular age-dependent transportation mechanism to target a suitable environment for viral replication.

scholarly journals Elimination of pepper mild mottle virus from infected tobacco (Nicotiana benthamiana L.) plants by callus culture and the sieving technique

2012 ◽  
Vol 48 (6) ◽  
pp. 595-599 ◽  
Author(s):  
Youngsun Kwon ◽  
Md. Alamgir Kabir ◽  
Hong Wei Wang ◽  
Thirupathi Karuppanapandian ◽  
Jun-Cheol Moon ◽  
...  
Keyword(s):  
Callus Culture ◽  
Nicotiana Benthamiana ◽  
Mottle Virus ◽  
Pepper Mild Mottle Virus ◽  
Infected Tobacco

scholarly journals Storage Root Yield of Sweetpotato as Influenced by Sweetpotato leaf curl virus and Its Interaction With Sweetpotato feathery mottle virus and Sweetpotato chlorotic stunt virus in Kenya

Plant Disease ◽  
2020 ◽  
Vol 104 (5) ◽  
pp. 1477-1486
Author(s):  
Bramwel W. Wanjala ◽  
Elijah M. Ateka ◽  
Douglas W. Miano ◽  
Jan W. Low ◽  
Jan F. Kreuze
Keyword(s):  
Yield Loss ◽  
Virus Disease ◽  
Block Design ◽  
Mottle Virus ◽  
Yield Response ◽  
Yield Reduction ◽  
Root Yield ◽  
Moderately Resistant ◽  
Leaf Curl Virus ◽  
Leaf Curl

In this study, the effect of a Kenyan strain of Sweetpotato leaf curl virus (SPLCV) and its interactions with Sweetpotato feathery mottle virus (SPFMV) and Sweetpotato chlorotic stunt virus (SPCSV) on root yield was determined. Trials were performed during two seasons using varieties Kakamega and Ejumula and contrasting in their resistance to sweetpotato virus disease in a randomized complete block design with 16 treatments replicated three times. The treatments included plants graft inoculated with SPLCV, SPFMV, and SPCSV alone and in possible dual or triple combinations. Yield and yield-related parameters were evaluated at harvest. The results showed marked differences in the effect of SPLCV infection on the two varieties. Ejumula, which is highly susceptible to SPFMV and SPCSV, suffered no significant yield loss from SPLCV infection, whereas Kakamega, which is moderately resistant to SPFMV and SPCSV, suffered an average of 47% yield loss from SPLCV, despite only mild symptoms occurring in both varieties. These results highlight the variability in yield response to SPLCV between sweetpotato cultivars as well as a lack of correlation of SPLCV-related symptoms with yield reduction. In addition, they underline the lack of correlation between resistance to the RNA viruses SPCSV and SPFMV and the DNA virus SPLCV. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

scholarly journals Pepper Mild Mottle Virus Coat Protein Alone Can Elicit the Capsicum spp. L3 Gene-Mediated Resistance

1998 ◽  
Vol 11 (12) ◽  
pp. 1253-1257 ◽  
Author(s):  
P. Gilardi ◽  
I. García-Luque ◽  
M. T. Serra
Keyword(s):  
Coat Protein ◽  
Resistance Gene ◽  
Protein Gene ◽  
Expression System ◽  
Potato Virus X ◽  
Mottle Virus ◽  
Capsicum Chinense ◽  
Pepper Mild Mottle Virus ◽  
Capsicum Spp ◽  
Virus Coat

The pepper mild mottle virus (PMMoV-S) (an L3 hypersensitive response [HR]-inducer strain) coat protein was expressed in Capsicum chinense (L3L3) plants with the heterologous potato virus X (PVX)-based expression system. The chimeric virus was localized in the inoculated leaves and induced the HR, thus indicating that the tobamoviral sequences that affect induction of the HR conferred by the L3 resistance gene reside in the coat protein gene. Furthermore, transient expression of the PMMoV-S coat protein in C. chinense leaves by biolistic co-bombardment with a plasmid expressing the β-glucuronidase (GUS) gene leads to the induction of cell death and expression of host defense genes. Thus, the coat protein of PMMoV-S is the elicitor of the Capsicum spp. L3 resistance gene-mediated HR.

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