scholarly journals Fresh, equilibrated and post-thaw sperm quality of Brycon orbignyanus (Valenciennes, 1850) and Prochilodus lineatus (Valenciennes, 1837) treated with either salmon GnRHa and domperidone or pituitary extract

2015 ◽  
Vol 13 (1) ◽  
pp. 157-164 ◽  
Author(s):  
Ana T.M. Viveiros ◽  
Antonio C.S. Gonçalves ◽  
Ariane F. Nascimento ◽  
Marcelo C. Leal
Keyword(s):  
Seminal Plasma ◽  
Volume Concentration ◽  
Sperm Quality ◽  
Plasma Osmolality ◽  
Pituitary Extract ◽  
Prochilodus Lineatus ◽  
Reduced Dose ◽  
Freezing Medium ◽  
Nitrogen Vapor

The effects of reduced doses of Ovaprim™ (GnRHa + domperidone) on sperm release of Brycon orbignyanus and Prochilodus lineatus were evaluated. Furthermore, sperm quality was compared among fresh, equilibrated and post-thaw samples. Males received a single and reduced dose of Ovaprim™ (0.125 or 0.25 ml/kg); control males received pituitary extract (cPE; 3 mg/kg). Fresh sperm was evaluated for volume, concentration, seminal plasma osmolality and seminal plasma pH. Then sperm was diluted in a freezing medium, equilibrated for 15-20 min and frozen in nitrogen vapor vessel (dry-shipper). Sperm motility was analyzed during 60 s post-activation in fresh, equilibrated and post-thaw samples. Sperm quality of males treated with Ovaprim™ (both doses) were not different from that of cPE-treated males, thus these data were pooled. In B. orbignyanus, motility was higher in fresh (99%) than in equilibrated sperm (81%); post-thaw motility dropped to 42%. In P. lineatus, motility was similar in fresh (99%) and equilibrated sperm (92%); post-thaw motility was 73%. Motility decreased as a function of time post-activation, and this decrease was significant after 60 s in fresh and equilibrated sperm, and as soon as 30 s in post-thaw sperm, in both species. Ovaprim™ at 1/4 of the recommended dose can successfully replace cPE.

Sperm dilution ratio affects post-thaw motility rate and velocity of Prochilodus lineatus (Characiformes) sperm

Zygote ◽  
2015 ◽  
Vol 24 (5) ◽  
pp. 662-667 ◽  
Author(s):  
Ana T.M. Viveiros ◽  
Marcelo C. Leal
Keyword(s):  
Sperm Quality ◽  
Aquatic Organisms ◽  
Computer Assisted ◽  
Dilution Ratio ◽  
Prochilodus Lineatus ◽  
Freezing Medium ◽  
Final Volume ◽  
Straight Line ◽  
Motility Rate

SummaryThere is a lack of standardization in sperm cryopreservation of aquatic organisms and, thus, a necessity of more accurate investigations in all steps of this process. In this study, the effects of sperm dilution ratio on post-thaw sperm quality of Prochilodus lineatus were evaluated. Sperm was diluted in a standard freezing medium (glucose and methyl glycol) at four different ratios (sperm to final volume = 1:5, 1:10, 1:50 or 1:100), frozen in a nitrogen vapour vessel at –170°C and then stored in liquid nitrogen vessel at –196°C. Post-thaw motility rate and velocities (curvilinear = VCL; average path = VAP; straight line = VSL) were determined using a Computer-Assisted Sperm Analyzer (CASA) at 10 and 40 s post-activation. The highest motility rates were observed when sperm was frozen at a ratio of 1:5 (76%) and 1:10 (75%). The highest VCL (225 μm/s) and VAP (203 μm/s) were observed at a ratio of 1:10, while VSL was similar among samples frozen at 1:5, 1:10 and 1:50 (97–124 μm/s). When those parameters were evaluated again 30 s later, motility decreased significantly in samples frozen at a ratio of 1:5 (57%) and 1:10 (61%), while velocities decreased significantly in all samples regardless of dilution ratio (75–85 μm/s of VCL, 38–53 μm/s of VAP and 25–39 μm/s of VSL). P. lineatus sperm should be frozen at a ratio of 1:10, where both the number of loaded sperm per straw and the post-thaw quality are maximized.

scholarly journals O papel do dimetilsulfóxido na criopreservação de sêmen de Curimba (Prochilodus lineatus)

2015 ◽  
Vol 36 (5) ◽  
pp. 3471
Author(s):  
Antonio Sergio Varela Junior ◽  
Estela Fernandes Silva ◽  
Tainã Figueiredo Cardoso ◽  
Érica Yokoyama Namba ◽  
Rodrigo Desessards Jardim ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Membrane Integrity ◽  
Fertilization Rate ◽  
Dna Integrity ◽  
Hatching Rate ◽  
Sperm Viability ◽  
Prochilodus Lineatus ◽  
Dmso Concentration ◽  
Fresh Semen

<p class="Pa7">Cryopreservation of Curimba semen (Prochilodus lineatus) is ecological and commercial importance. The objective of this study was to evaluate the effect of different concentrations (2, 5, 8 and 11%) of dimethyl sulfoxide (DMSO) diluted in Betsville Thawing Solution (BTS) on the quality of post-thaw semen Curimba. We analyzed the rate and period motility, sperm viability, membrane integrity and DNA, mitochondrial functionality, and fertilization and hatching rate. The plasma membrane and DNA integrity of a DMSO concentration of 11% obtained better results than the concentration of 5% (p &lt;0.05). However, treatment of 5% DMSO resulted in a longer latency and a higher fertilization rate and hatching, in other sperm quality equal to that of fresh semen. The results of this study indicate that 5% DMSO is ideal for cryopreservation of semen Curimba.</p>

scholarly journals Assessment of sperm quality and short term preservation of sperm in grey mullet Mugil cephalus Linnaeus, 1758

2016 ◽  
Vol 63 (4) ◽  
Author(s):  
Balamurugan Ramachandran ◽  
Munuswamy Natesan
Keyword(s):  
Marine Fish ◽  
Seminal Plasma ◽  
Sperm Quality ◽  
Plasma Osmolality ◽  
Ringer Solution ◽  
Mugil Cephalus ◽  
Grey Mullet ◽  
Short Term ◽  
Sperm Density ◽  
Two Samples

Quality of fresh milt of grey mullet Mugil cephalus Linnaeus, 1758 was assessed based on motility grade, motility duration, sperm count and seminal plasma osmolality. Freshly collected milt from live males (n=17) were examined individually for motility assessment. Motility grade was four in fifteen samples whereas three in other two samples analysed. Other sperm quality parameters such as motility duration, sperm density and seminal plasma osmolality were : 421.38±137 sec, 1.67±0.3x108 nos. ml-1 and 362.28±79.13 m Osmol kg-1 respectively. To standardise short term preservation of sperm, four different extenders such as ringer solution for marine fish, modified marine fish ringer solution, V2E extender and honey in fish ringer solution were experimented. The results indicated that short term preservation at 4oC over a period of 10 days with V2E extender at 1:1 ratio significantly (p<0.05) retained the highest sperm motility grade (2.6±0.5) over other dilutions such as 1:2 and 1:3 on 5th day. However, on 10th day of observation, sperms were immotile after activation with seawater, in undiluted as well as diluted milt samples. This probably indicates that V2E extender is effective for short term preservation of sperm in grey mullet.

scholarly journals Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm

2014 ◽  
Vol 12 (3) ◽  
pp. 643-648 ◽  
Author(s):  
Ana T. M. Viveiros ◽  
Tais R. Taffarel ◽  
Marcelo C. Leal
Keyword(s):  
Cold Storage ◽  
Sperm Quality ◽  
The Other ◽  
Coconut Water ◽  
Prochilodus Lineatus ◽  
Straight Line ◽  
And Control ◽  
Motility Rate

This study evaluates the effects of extender osmolality and composition on the cooling of Prochilodus lineatussperm. Sperm was diluted in six extenders: two compositions (powdered coconut water(tm) = ACP; Beltsville Thawing Solution(tm) = BTS) x three osmolalities (285, 325, and 365 mOsm/kg) plus an undiluted control, and stored at 6-8°C. Motility rate and velocities (curvilinear, straight line, and average path) were determined every other day. Osmolality did not affect the quality of cooled sperm, thus data were pooled. Motility was higher on d 0 compared to the other days and diluted samples (85-90%) yielded higher motility than control (75%). On d 2, motility was higher in BTS-diluted samples and control, but on d 4 and 6, control yielded the highest motility. Velocities decreased from d 0 to 6 in diluted samples, but not in control. On d 0, velocities were higher in BTS-diluted sperm, but, on d 2, 4, and 6, control yielded higher velocities despite of the large variation among males. Thus P. lineatussperm should be stored in BTS or without dilution, for a maximum of two days at 6-8°C. Extender osmolality between 285 and 365 mOsm/kg does not affect sperm quality during cold storage.

scholarly journals Effect of Centrifugation on Motility, Sperm Capacitation and Acrosome Reaction in Soy Bean and Avocado Seed Milk Extenders of Cryopreserved Goat Spermatozoa

2017 ◽  
Vol 50 (1) ◽  
pp. 13-18
Author(s):  
James Ola Daramola
Keyword(s):  
Sperm Motility ◽  
Seminal Plasma ◽  
Acrosome Reaction ◽  
Sperm Quality ◽  
West African ◽  
West African Dwarf ◽  
Artificial Vagina ◽  
Avocado Seed

Abstract Removal of seminal plasma by centrifugation (0 centrifugation, 1 centrifugation, 2 centrifugations, 3 centrifugations) and preservation in two different tris-extenders viz., avocado seed milk (ASM) and soy bean milk (SBM) based extenders were studied for their ability to support motility, in vitro capacitation and acrosome reaction of spermatozoa obtained from West African Dwarf (WAD) goat bucks during cryopreservation. Semen samples collected with the aid of artificial vagina were centrifuged for one, two and three times. The centrifuged samples were diluted with the two tris-extenders each containing 20 mL of avocado seed milk and soybean milk and cryopreserved for 30 days. The results showed higher (P < 0.05) sperm motility (P < 0.05) with increased centrifugation times. Spermatozoa that were centrifuged had higher (P < 0.05) percentage of acrosome reaction and capacitation with increased centrifugation times compared to the control. Optimal improvement in these parameters was obtained with increased centrifugation times. The findings revealed that removal of seminal plasma by centrifugation improved sperm quality of WAD goat bucks during cryopreservation and optimum improvement was achieved consistently with 3 centrifugations.

Gamete quality of streaked prochilod Prochilodus lineatus (Characiformes) after GnRHa and dopamine antagonist treatment

Zygote ◽  
2013 ◽  
Vol 23 (2) ◽  
pp. 212-221 ◽  
Author(s):  
Ana T.M. Viveiros ◽  
Antônio C.S. Gonçalves ◽  
Isabela M. Di Chiacchio ◽  
Ariane F. Nascimento ◽  
Elizabeth Romagosa ◽  
...  
Keyword(s):  
Sperm Quality ◽  
Fertilization Rate ◽  
Dopamine Antagonist ◽  
Hatching Rate ◽  
Oocyte Diameter ◽  
Prochilodus Lineatus ◽  
Mean Values ◽  
Straight Line ◽  
The Mean

SummaryThe efficiency of Ovaprim™ salmon gonadotropin-releasing hormone agonist (GnRHa) and dopamine antagonist on the induction of spawning and spermiation in Prochilodus lineatus in comparison with the commonly used method using pituitary extract (PE) was evaluated. Females received PE at 0.5 + 5.0 mg/kg and Ovaprim™ at 0.05 + 0.45 ml/kg or at 0.125 + 0.375 ml/kg. All males received a first dose of PE at 0.4 mg/kg and then PE at 4.0 mg/kg or Ovaprim™ at 0.25 ml/kg. Oocyte, egg, larvae and sperm quality were evaluated. All females spawned and oocyte, egg and larvae quality were similar between Ovaprim™-treated (both doses) and PE-treated females. Data from females were pooled and the mean values were: 242 g ova weight, 15% ova index, 1209 oocytes/g ova, 284,539 oocytes/female, 183 oocytes/g body weight, 1.18 mm oocyte diameter, 49% fertilization rate, 43% hatching rate and 89% normal larvae. Sperm quality was similar between Ovaprim™-treated and PE-treated males. Data from males were pooled and the mean values of semen were: volume of 3.0 ml, 14.9 × 109 sperm/ml, osmolality of 283 mOsm/kg, pH of 7.4, 71% motile sperm, 217 μm/s curvilinear velocity, 102 μm/s straight-line velocity and 189 μm/s average path velocity. Ovaprim™ treatment can be used for commercial reproduction of P. lineatus, without any loss of gamete quality in comparison with PE treatment.

Qualitative indicators of sperm bulls of spermogonial jsc "krasnoyarskugol"

2018 ◽  
Vol 22 (03) ◽  
pp. 26-28
Author(s):  
Е V Chetvertakova ◽  
Y V Abaza
Keyword(s):  
Sperm Motility ◽  
Volume Concentration ◽  
Sperm Quality ◽  
Sperm Donor

Sperm quality is one of the most important criteria for bull manufacturer is used in the mode of the sperm donor was a. On spermatogenesis can affect both genotypical and paratypical factors. The quality of sperm is determined by ejaculate volume, concentration, sperm motility and morphology.

Effect of new dietary supplement on sperm quality

2012 ◽  
Vol 153 (45) ◽  
pp. 1787-1792 ◽  
Author(s):  
Mária Horváth ◽  
Endre Czeizel
Keyword(s):  
Adverse Effect ◽  
Clinical Trial ◽  
Dietary Supplement ◽  
Male Fertility ◽  
Sperm Quality ◽  
Sperm Count ◽  
Before And After ◽  
The Usa ◽  
New Treatments

Introduction: There is a decline in male fertility thus new treatments are needed. Aims: To test the efficacy of a new dietary supplement developed in the USA and registered as a curing drug in Hungary (OGYI). Methods: In a clinical trial 100 men with low sperm quality (spermium count 5–20 M/ml, good motility 10–40%, and adverse shape 30–50%) were examined. Results: Sperm parameters were measured before and after a 3-month treatment and after another 3-month without treatment. This dietary supplement statistically and clinically significantly improved sperm count and motility. In 74 cases this dietary supplement demonstrated a beneficial effect on sperm quality (more than 10% increase in sperm count, or quality of motility, or shape); in 16 cases the improvement exceeded 30%. No adverse effect could be accounted for this treatment. Conclusions: This new dietary supplement may contribute to the treatment of male infertility. Orv. Hetil., 2012, 153, 1787–1792.

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