scholarly journals Osmolality and composition of the extender during the cold storage of Prochilodus lineatus (Characiformes: Prochilodontidae) sperm

2014 ◽  
Vol 12 (3) ◽  
pp. 643-648 ◽  
Author(s):  
Ana T. M. Viveiros ◽  
Tais R. Taffarel ◽  
Marcelo C. Leal

This study evaluates the effects of extender osmolality and composition on the cooling of Prochilodus lineatussperm. Sperm was diluted in six extenders: two compositions (powdered coconut water(tm) = ACP; Beltsville Thawing Solution(tm) = BTS) x three osmolalities (285, 325, and 365 mOsm/kg) plus an undiluted control, and stored at 6-8°C. Motility rate and velocities (curvilinear, straight line, and average path) were determined every other day. Osmolality did not affect the quality of cooled sperm, thus data were pooled. Motility was higher on d 0 compared to the other days and diluted samples (85-90%) yielded higher motility than control (75%). On d 2, motility was higher in BTS-diluted samples and control, but on d 4 and 6, control yielded the highest motility. Velocities decreased from d 0 to 6 in diluted samples, but not in control. On d 0, velocities were higher in BTS-diluted sperm, but, on d 2, 4, and 6, control yielded higher velocities despite of the large variation among males. Thus P. lineatussperm should be stored in BTS or without dilution, for a maximum of two days at 6-8°C. Extender osmolality between 285 and 365 mOsm/kg does not affect sperm quality during cold storage.

Zygote ◽  
2015 ◽  
Vol 24 (5) ◽  
pp. 662-667 ◽  
Author(s):  
Ana T.M. Viveiros ◽  
Marcelo C. Leal

SummaryThere is a lack of standardization in sperm cryopreservation of aquatic organisms and, thus, a necessity of more accurate investigations in all steps of this process. In this study, the effects of sperm dilution ratio on post-thaw sperm quality of Prochilodus lineatus were evaluated. Sperm was diluted in a standard freezing medium (glucose and methyl glycol) at four different ratios (sperm to final volume = 1:5, 1:10, 1:50 or 1:100), frozen in a nitrogen vapour vessel at –170°C and then stored in liquid nitrogen vessel at –196°C. Post-thaw motility rate and velocities (curvilinear = VCL; average path = VAP; straight line = VSL) were determined using a Computer-Assisted Sperm Analyzer (CASA) at 10 and 40 s post-activation. The highest motility rates were observed when sperm was frozen at a ratio of 1:5 (76%) and 1:10 (75%). The highest VCL (225 μm/s) and VAP (203 μm/s) were observed at a ratio of 1:10, while VSL was similar among samples frozen at 1:5, 1:10 and 1:50 (97–124 μm/s). When those parameters were evaluated again 30 s later, motility decreased significantly in samples frozen at a ratio of 1:5 (57%) and 1:10 (61%), while velocities decreased significantly in all samples regardless of dilution ratio (75–85 μm/s of VCL, 38–53 μm/s of VAP and 25–39 μm/s of VSL). P. lineatus sperm should be frozen at a ratio of 1:10, where both the number of loaded sperm per straw and the post-thaw quality are maximized.


Zygote ◽  
2013 ◽  
Vol 23 (2) ◽  
pp. 212-221 ◽  
Author(s):  
Ana T.M. Viveiros ◽  
Antônio C.S. Gonçalves ◽  
Isabela M. Di Chiacchio ◽  
Ariane F. Nascimento ◽  
Elizabeth Romagosa ◽  
...  

SummaryThe efficiency of Ovaprim™ salmon gonadotropin-releasing hormone agonist (GnRHa) and dopamine antagonist on the induction of spawning and spermiation in Prochilodus lineatus in comparison with the commonly used method using pituitary extract (PE) was evaluated. Females received PE at 0.5 + 5.0 mg/kg and Ovaprim™ at 0.05 + 0.45 ml/kg or at 0.125 + 0.375 ml/kg. All males received a first dose of PE at 0.4 mg/kg and then PE at 4.0 mg/kg or Ovaprim™ at 0.25 ml/kg. Oocyte, egg, larvae and sperm quality were evaluated. All females spawned and oocyte, egg and larvae quality were similar between Ovaprim™-treated (both doses) and PE-treated females. Data from females were pooled and the mean values were: 242 g ova weight, 15% ova index, 1209 oocytes/g ova, 284,539 oocytes/female, 183 oocytes/g body weight, 1.18 mm oocyte diameter, 49% fertilization rate, 43% hatching rate and 89% normal larvae. Sperm quality was similar between Ovaprim™-treated and PE-treated males. Data from males were pooled and the mean values of semen were: volume of 3.0 ml, 14.9 × 109 sperm/ml, osmolality of 283 mOsm/kg, pH of 7.4, 71% motile sperm, 217 μm/s curvilinear velocity, 102 μm/s straight-line velocity and 189 μm/s average path velocity. Ovaprim™ treatment can be used for commercial reproduction of P. lineatus, without any loss of gamete quality in comparison with PE treatment.


2015 ◽  
Vol 75 (3) ◽  
pp. 662-669 ◽  
Author(s):  
EG Sanches ◽  
IR Oliveira ◽  
PCS Serralheiro ◽  
VR Cerqueira

AbstractThis study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C –min), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C –min, 1 minute of equilibration time and 1:3 (v/v) dilution ratio. The use of cryopreserved sperm presented fertilization rates >60% validating the present protocol for lane snapper. The cryoconserved sperm of lane snapper is a viable alternative, being possible to maintain appropriate sperm viability.


2015 ◽  
Vol 36 (5) ◽  
pp. 3471
Author(s):  
Antonio Sergio Varela Junior ◽  
Estela Fernandes Silva ◽  
Tainã Figueiredo Cardoso ◽  
Érica Yokoyama Namba ◽  
Rodrigo Desessards Jardim ◽  
...  

<p class="Pa7">Cryopreservation of Curimba semen (Prochilodus lineatus) is ecological and commercial importance. The objective of this study was to evaluate the effect of different concentrations (2, 5, 8 and 11%) of dimethyl sulfoxide (DMSO) diluted in Betsville Thawing Solution (BTS) on the quality of post-thaw semen Curimba. We analyzed the rate and period motility, sperm viability, membrane integrity and DNA, mitochondrial functionality, and fertilization and hatching rate. The plasma membrane and DNA integrity of a DMSO concentration of 11% obtained better results than the concentration of 5% (p &lt;0.05). However, treatment of 5% DMSO resulted in a longer latency and a higher fertilization rate and hatching, in other sperm quality equal to that of fresh semen. The results of this study indicate that 5% DMSO is ideal for cryopreservation of semen Curimba.</p>


1993 ◽  
Vol 23 (8) ◽  
pp. 1711-1715 ◽  
Author(s):  
S.M. Berch ◽  
A.L. Roth

Ectomycorrhizal colonization of container-grown Pseudotsugamenziesii (Mirb.) Franco (Douglas-fir) inoculated with Rhizopogonvinicolor A.H. Smith was determined after cold storage and after one growing season on a clearcut on eastern Vancouver Island. Inoculated Douglas-fir seedlings were taller than noninoculated controls when outplanted but, perhaps because of browse damage, no growth differences were found after one field season. Rhizopogonvinicolor colonized all of the inoculated but none of the control seedlings examined after cold storage. Volunteer Thelephoraterrestris Fr. colonized almost half of the control and 10% of the inoculated seedlings before outplanting. After one field season, inoculated and control seedlings were colonized by 15 ectomycorrhizal fungi each, only eight of which were found on both. Rhizopogonvinicolor persisted on the roots of inoculated plants, but was also present in the field soil since the control seedlings also bore these mycorrhizae after one growing season. The relative abundance of T. terrestris decreased from the nursery to the field. The other common ectomycorrhizae in the field included Myceliumradicisatrovirens Melin, Cenococcumgeophilum Fr., and types resembling Tuber and Endogone.


1955 ◽  
Vol 27 (1) ◽  
pp. 221-228
Author(s):  
Martti Sipilä

In carrying out a time study in the field of agricultural work it is very rarely that situations occur in which the outward conditions which affect the work could be regarded as completely invariable and in which the time spent on the work would be the only varying factor to be recorded. On the contrary, most often quite a number of different factors have an effect on the carrying out of the work, so many factors, indeed, that only the most important of them can be taken into account when carrying out time study. Almost without exception, therefore, a preliminary study must be made first, its aim being to clear up the most important of the factors affecting the work and to determine which of them should be taken into account in the final study. Furthermore, the preliminary study determins the measurement of the affecting factors either with objective measuring methods or by creating a classification, based on a subjective estimate made by a time study engineer, which can be considered sufficiently clear and practicable. In the study now in hand the thinning of sugar-beet with a shortshafted hoe, using the pull push method a preliminary study has been made of the work performed by several men on one day. On the basis of the preliminary study the following factors affecting the work were finally chosen to be included: the average length of the seedlings, the quality of the surface of the soil, abundance of weeds in the soil, and the number of the seedlings left at the thinning. As the unit used in the study the work done on 10 linear metres was chosen, and the determination of the different factors was correspondingly fixed. For the estimation of the abundance of weeds in the soil the following classification was made: I Class no weeds II » weeds in places III » some weeds IV » a moderate number of weeds V » weeds in abundance The quality of the surface of the soil was divided into three classes: I Class loose II » clods, somewhat encrusted III » clods, very encrusted, the soil hard. Furthermore, special instructions were given in regard to both of the aforementioned classifications, and their application was made uniform by the group training of the persons collecting the material. The length of the seedlings was measured by taking samples from three points on the unit under observation, and the mean average was computed. The number of the seedlings left at the thinning was taken from each research unit as the sum total. For the mathematical treatment of the material, the different factors were given symbols as follows: x1 = time spent on work/10 linear metres x2 = average length of seedlings/10 linear metres x3 = quality of surface of soil/10 linear metres x4 = abundance of weeds in soil/10 linear metres x5 = number of seedlings left at thinning/10 linear metres The statistically-mathematical treatment of the material is based on the hypothesis, reached on the basis of a preliminary study, that the time x1 is linearly dependent on the factors x2, x3, x4 and x5 which affect the work. The phenomenon may be described by a straight line the equation of which has the form: x1 = a + b * x2 + c * x3 + d * x4 + e * x5 and shows the simultaneous variation of the different arguments which affect the work. On the basis of the characteristics computed from the primary material the estimated final value of the aforementioned regression line of the work is: x1 = 0.15 + 0.05 * x2 + 0.40 * x3 + 0.47 * x4 + 0.05 * x5. To allow comparison the mathematical treatment was carried out, also by using a partial correlation computation. The coefficients of the correlation series are shown on page 224. In the series of the final coefficients of correlation r12x345 = 0.10 r13x245 = 0.33 r14x235 = 0.63 r15x234 = 0.35 the significance of the value r12x315 = 0.10 is so small that it cannot be regarded as probable. The other values, on the other hand, describe the phenomenon with an accuracy probability of 95%. To check the mathematical treatment on the basis of the aforementioned coefficients of correlation, the values of the coefficients a, b, c, d and e in the equation x1 = a + bx2 + cx3 + dx4 + ex5 have been computed and a result has been arrived at which is completely consistent with the result reached by the computation of the regression line. For practical application, working time values/10 linear metres, which are in accordance with the straight line obtained, have been computed on to Table No. 1, page 225. Also the additional 7.5 % have been taken into consideration in the calculations as corresponding to accepted interruptions, in accordance with the preliminary study. Since the importance of the lenght of the seedlings is of very little significance and not even probable, it has not been taken into consideration. The computations have been made as corresponding to a length of 5 cm. of the seedlings. Table No. 1 shows the money values which are in accordance with the time values on Table No. 1 and which keep to the basis agreed upon at the negotiations between the organizations on the labour-market. As the work in question has been work on a contract a 30 % increase for the contract has been agreed upon to be observed. Table No. 2 has been computed according to wages of 80 mks. per hour and the prices have been stated in pennies as the price paid for work per metre. The increase corresponding to the contract has been allowed in the figures.


2021 ◽  
Vol 10 (2) ◽  
pp. 147
Author(s):  
Khusaini Khusaini ◽  
Ali Sandy Mulya

<p class="JurnalASSETSABSTRAK"><strong>ABSTRACT</strong></p><p>This research aims to measure expectations, gender, learning quality, and control variables on the performance of the undergraduate program of Accounting at Tangerang Municipality. The target population is 1,898 students, with 331 of them becoming the sample. The sampling technique was Disproportionate Random Sampling and used a Binary Probit Regression. The distribution of the questionnaires used a Google Form. The results showed that the expectations and quality of learning had a significant effect on improving performance. On the other hand, gender was insignificant. This study's findings contribute to universities always maintaining student expectations and the quality of learning so that graduates have good performance.</p><p class="JurnalASSETSABSTRAK"><strong><em>ABSTRAK</em></strong><em></em></p><p><em>Penelitian ini bertujuan untuk menguji harapan, gender, kualitas pembelajaran, dan variabel kontrol terhadap kinerja mahasiswa program sarjana S-1 Akuntansi di Perguruan Tinggi Kota Tangerang. Populasi sebanyak 1.898 mahasiswa dan ukuran sampel sebanyak 331 sampel. Teknik sampling adalah </em>disproportionate random sampling<em> dan menggunakan analisis </em>binary probit regression<em>. Distribusi kuesioner menggunakan google form. Hasil penelitian menunjukkan bahwa harapan dan kualitas pembelajaran berpengaruh signifikan terhadap peningkatan kinerja mahasiswa, sebaliknya gender tidak signifikan. Temuan penelitian ini memberikan konstribusi kepada perguruan tinggi untuk selalu menjaga ekspektasi mahasiswa dan mutu pembelajaran agar para lulusannya memiliki kinerja yang baik.</em></p>


2015 ◽  
Vol 13 (1) ◽  
pp. 157-164 ◽  
Author(s):  
Ana T.M. Viveiros ◽  
Antonio C.S. Gonçalves ◽  
Ariane F. Nascimento ◽  
Marcelo C. Leal

The effects of reduced doses of Ovaprim™ (GnRHa + domperidone) on sperm release of Brycon orbignyanus and Prochilodus lineatus were evaluated. Furthermore, sperm quality was compared among fresh, equilibrated and post-thaw samples. Males received a single and reduced dose of Ovaprim™ (0.125 or 0.25 ml/kg); control males received pituitary extract (cPE; 3 mg/kg). Fresh sperm was evaluated for volume, concentration, seminal plasma osmolality and seminal plasma pH. Then sperm was diluted in a freezing medium, equilibrated for 15-20 min and frozen in nitrogen vapor vessel (dry-shipper). Sperm motility was analyzed during 60 s post-activation in fresh, equilibrated and post-thaw samples. Sperm quality of males treated with Ovaprim™ (both doses) were not different from that of cPE-treated males, thus these data were pooled. In B. orbignyanus, motility was higher in fresh (99%) than in equilibrated sperm (81%); post-thaw motility dropped to 42%. In P. lineatus, motility was similar in fresh (99%) and equilibrated sperm (92%); post-thaw motility was 73%. Motility decreased as a function of time post-activation, and this decrease was significant after 60 s in fresh and equilibrated sperm, and as soon as 30 s in post-thaw sperm, in both species. Ovaprim™ at 1/4 of the recommended dose can successfully replace cPE.


2004 ◽  
Vol 47 (5) ◽  
pp. 799-804 ◽  
Author(s):  
Simone Marques ◽  
Hugo Pereira Godinho

Sperm of the following Neotropical Characiformes fish species were tested for cold storage: Brycon lundii, Piaractus mesopotamicus, Leporinus elongatus, Leporinus friderici, Prochilodus lineatus and Prochilodus marggravii. Each sperm sample was split into two aliquots. The first was placed into a plastic bag with air or oxygen and the second, in a plastic tube with air. The samples were maintained at temperatures between 1.7-4.9 ºC. The rate of sperm motility was estimated using a 50 mM NaCl solution as the activating solution. The shortest sperm storage duration (7 h) was recorded for L. friderici, when the sperm motility rate reached ~ 30%, whereas the longest duration (20 h) was obtained with the sperm of P. lineatus. A fertilisation test using Prochilodus marggravii sperm refrigerated for 8 h yielded 88-90% of viable embryos. The refrigerated storage method could be of practical applications, especially in fish reproductive management at hatchery stations.


2019 ◽  
Vol 35 (3) ◽  
pp. 747-753 ◽  
Author(s):  
Luana Barbosa Pires ◽  
Eduardo Antonio Sanches ◽  
Elizabeth Romagosa ◽  
Ruy Alberto Caetano Corrêa Filho ◽  
Rosiane Araujo Rodrigues Nass ◽  
...  

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