O papel do dimetilsulfóxido na criopreservação de sêmen de Curimba (Prochilodus lineatus)

Cryopreservation of Curimba semen (Prochilodus lineatus) is ecological and commercial importance. The objective of this study was to evaluate the effect of different concentrations (2, 5, 8 and 11%) of dimethyl sulfoxide (DMSO) diluted in Betsville Thawing Solution (BTS) on the quality of post-thaw semen Curimba. We analyzed the rate and period motility, sperm viability, membrane integrity and DNA, mitochondrial functionality, and fertilization and hatching rate. The plasma membrane and DNA integrity of a DMSO concentration of 11% obtained better results than the concentration of 5% (p <0.05). However, treatment of 5% DMSO resulted in a longer latency and a higher fertilization rate and hatching, in other sperm quality equal to that of fresh semen. The results of this study indicate that 5% DMSO is ideal for cryopreservation of semen Curimba.

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Application of different honey and dilution ratios on sperm quality of Pangasianodon hypopthalmus

Jurnal Akuakultur Indonesia ◽

10.19027/jai.14.164-170 ◽

2015 ◽

Vol 14 (2) ◽

pp. 164

Author(s):

Harton Arfah ◽

Fahmi Hasan ◽

Mia Setiawati

Keyword(s):

Sperm Quality ◽

Storage Period ◽

Fertilization Rate ◽

Hatching Rate ◽

Sperm Viability ◽

Dilution Ratio ◽

Pangasianodon Hypophthalmus

ABSTRACT The aim of this study was to determine the effect of various types of honey (longan honey, lychee honey, and cottonwoods honey) on sperm quality of Siamese catfish Pangasianodon hypopthalmus with different dilution ratios after the storage period which includes sperm viability, fertilization rate, and hatching rate. The best treatment was obtained on the aplication of longan honey as a sperm diluent at 1:50 dilution ratio, with sperm viability 96.33±0.58%, fertilization rate 97.10±0.70%, and hatching rate 93.44±2.39%. Keywords: Siamese catfish, Pangasianodon hypophthalmus, honey, sperm dilution ratio ABSTRAK Tujuan penelitian ini adalah mengevaluasi pengaruh pemberian berbagai jenis madu (madu kelengkeng, madu leci, dan madu randu) terhadap kualitas sperma ikan patin siam Pangasianodon hypopthalmus dengan rasio pengenceran berbeda setelah masa penyimpanan, yang meliputi viabilitas sperma, tingkat fertilisasi, dan derajat penetasan. Hasil penelitian menunjukkan bahwa perlakuan terbaik didapatkan pada pemberian madu kelengkeng dengan rasio pengenceran 1:50 dengan viabilitas sperma 96,33±0,58%, tingkat fertilisasi 97,10±0,70%, dan derajat penetasan 93,44±2,39%. Kata kunci: ikan patin, Pangasianodon hypopthalmus, madu, rasio pengenceran sperma

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Gamete quality of streaked prochilod Prochilodus lineatus (Characiformes) after GnRHa and dopamine antagonist treatment

Zygote ◽

10.1017/s0967199413000440 ◽

2013 ◽

Vol 23 (2) ◽

pp. 212-221 ◽

Cited By ~ 11

Author(s):

Ana T.M. Viveiros ◽

Antônio C.S. Gonçalves ◽

Isabela M. Di Chiacchio ◽

Ariane F. Nascimento ◽

Elizabeth Romagosa ◽

...

Keyword(s):

Sperm Quality ◽

Fertilization Rate ◽

Dopamine Antagonist ◽

Hatching Rate ◽

Oocyte Diameter ◽

Prochilodus Lineatus ◽

Mean Values ◽

Straight Line ◽

The Mean

SummaryThe efficiency of Ovaprim™ salmon gonadotropin-releasing hormone agonist (GnRHa) and dopamine antagonist on the induction of spawning and spermiation in Prochilodus lineatus in comparison with the commonly used method using pituitary extract (PE) was evaluated. Females received PE at 0.5 + 5.0 mg/kg and Ovaprim™ at 0.05 + 0.45 ml/kg or at 0.125 + 0.375 ml/kg. All males received a first dose of PE at 0.4 mg/kg and then PE at 4.0 mg/kg or Ovaprim™ at 0.25 ml/kg. Oocyte, egg, larvae and sperm quality were evaluated. All females spawned and oocyte, egg and larvae quality were similar between Ovaprim™-treated (both doses) and PE-treated females. Data from females were pooled and the mean values were: 242 g ova weight, 15% ova index, 1209 oocytes/g ova, 284,539 oocytes/female, 183 oocytes/g body weight, 1.18 mm oocyte diameter, 49% fertilization rate, 43% hatching rate and 89% normal larvae. Sperm quality was similar between Ovaprim™-treated and PE-treated males. Data from males were pooled and the mean values of semen were: volume of 3.0 ml, 14.9 × 109 sperm/ml, osmolality of 283 mOsm/kg, pH of 7.4, 71% motile sperm, 217 μm/s curvilinear velocity, 102 μm/s straight-line velocity and 189 μm/s average path velocity. Ovaprim™ treatment can be used for commercial reproduction of P. lineatus, without any loss of gamete quality in comparison with PE treatment.

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The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v12i1.22353 ◽

2020 ◽

Vol 12 (1) ◽

pp. 125-131

Author(s):

Raudhah Mahfudhah ◽

Kartini Eriani ◽

Zainal Abidin Muchlisin ◽

Cut Ruhul Muthmainnah

Keyword(s):

Plasma Membrane ◽

Clarias Gariepinus ◽

Membrane Integrity ◽

Dna Integrity ◽

Freezing And Thawing ◽

Randomized Design ◽

Fetal Bovine ◽

Fresh Semen ◽

Different Doses

The cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent. This study aimed to examine the effect of the addition of different doses of glutathione in cryopreservation of Aceh Local catfish (Clarias gariepinus) spermatozoa after freezing. The method used was a completely randomized design (CRD) with four treatments and four replications. Fresh semen was diluted in Ringer, 15% DMSO, and 20% Fetal Bovine Serum (FBS) and then added with glutathione antioxidants of 0.0 mgL-1, 0.5 mgL-1, 1.0 mgL-1, and 2.0 mgL-1. The parameters observed in this study were motility, integrity of the plasma membrane, fertility, and DNA integrity. The results showed that the concentration of glutathione had no effect on motility, integrity ofthe plasma membrane, or fertility, but had an effect on DNA integrity. The highest percentage of motility and plasma membrane integrity respectively was 40.50% (P3) and 70.87% (P2). Furthermore, the assessment of DNA integrity showed that there was no DNA fragmentation both treatments and fresh spermatozoa. This research is the first study regarding glutathione supplementation in cryo-diluent of Aceh Local catfish spermatozoa. Finally, the results obtained can provide information about the exact concentration of glutathione in the extender on the quality of spermatozoa of Aceh Local catfish (C. gariepinus) after the cryopreservation process. These results can also increase the success of fertility be used by the seed hall unit and the aquaculture industry to increase the productivity and supply high quality seeds.

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Metformin Improves Quality of Post-Thaw Canine Semen

Animals ◽

10.3390/ani10020287 ◽

2020 ◽

Vol 10 (2) ◽

pp. 287 ◽

Cited By ~ 2

Author(s):

Jérémy Grandhaye ◽

Agnieszka Partyka ◽

Zuzanna Ligocka ◽

Agata Dudek ◽

Wojciech Niżański ◽

...

Keyword(s):

Oxidative Stress ◽

Sperm Quality ◽

Membrane Integrity ◽

Fertilization Rate ◽

Mitochondrial Activity ◽

Assisted Reproductive Technique ◽

Dna Damages ◽

Semen Extender ◽

Metabolic Activities

Sperm cryopreservation is an assisted reproductive technique routinely used in canine species for genetic conservation. However, during cryopreservation, the DNA damages are still elevated, limiting the fertilization rate. The present study was conducted to evaluate whether supplementation of canine semen extender with a molecule limiting the metabolic activities can improve the quality of frozen-thawed canine spermatozoa. We used metformin, known to limit the mitochondrial respiratory and limit the oxidative stress. Before and during the freezing procedure, metformin (50µM and 500µM) has been added to the extender. After thawing, sperm exposed to metformin conserved the same viability without alteration in the membrane integrity or acrosome reaction. Interestingly, 50µM metformin improved the sperm motility in comparison to the control, subsequently increasing mitochondrial activity and NAD+ content. In addition, the oxidative stress level was reduced in sperm treated with metformin improving the sperm quality as measured by a different molecular marker. In conclusion, we have shown that metformin is able to improve the quality of frozen-thawed dog semen when it is used during the cryopreservative procedure.

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Effects of Cryopreservation on Gene Expression and Post Thaw Sperm Quality of Pacific Abalone, Haliotis discus hannai

Frontiers in Marine Science ◽

10.3389/fmars.2021.652390 ◽

2021 ◽

Vol 8 ◽

Author(s):

Shaharior Hossen ◽

Zahid Parvez Sukhan ◽

Yusin Cho ◽

Kang Hee Kho

Keyword(s):

Gene Expression ◽

Mrna Expression ◽

Sperm Quality ◽

Membrane Integrity ◽

Dna Integrity ◽

Haliotis Discus Hannai ◽

Pacific Abalone ◽

Haliotis Discus

Pacific abalone, Haliotis discus hannai, is a high commercial seafood in South-East Asia. The aim of the present study was to determine effects of cryopreservation on gene expression and post thaw sperm quality of Pacific abalone. Two ions, Na+ (459.1 ± 3.1 mM) and Cl– (515.9 ± 1.1 mM), were predominant in the seminal plasma (pH: 6.8 ± 0.1; osmolarity: 1,126 ± 3 mOsmL–1). Cryopreservation reduced mRNA expression levels of protein kinase A (PKA-C) and heat shock proteins (HSP70 and HSP90) genes in sperm. Fluorescent technique was used to compare morphological defects, acrosome integrity (AI), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), and DNA integrity of sperm cryopreserved with five different cryopreservation solutions (8% Me2SO, 8% EG, 6% PG, 2% GLY, and 2% MeOH). Droplet in tail and coiled tail defects was not observed for sperm cryopreserved with 8% Me2SO or 2% GLY. Sperm cryopreserved with 8% Me2SO showed improved DNA integrity and lower cryodamage than sperm cryopreserved with other cryoprotectants. Sperm to egg ratio of 10,000:1 was found to be the most suitable ratio for in vitro fertilization among different ratios tested. The fertilization rate of sperm cryopreserved with 8% Me2SO was not significantly (p > 0.05) different from that of sperm cryopreserved with 2% GLY. DNA fragmentation showed strongly negative relationships with sperm quality parameters. Sperm cryopreserved with 8% Me2SO showed higher post thaw quality and mRNA expression of sperm motility associated gene than those cryopreserved with other cryoprotectants. The present research suggests to use 8% Me2SO for cryopreservation of Pacific abalone sperm as well as for hatchery production.

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Potential of Flamboyant Flower (Delonix regia (Boj. ex Hook) Raf) Extract to Maintain Post-Dilution Quality of Aceh Cattle (Bos taurus indicus) Spermatozoa

E3S Web of Conferences ◽

10.1051/e3sconf/202015101044 ◽

2020 ◽

Vol 151 ◽

pp. 01044

Author(s):

Marlina Muhammad ◽

Kartini Eriani ◽

Aida Fithri ◽

Amalia Rusdi ◽

Hendra Saputra

Keyword(s):

Artificial Insemination ◽

Bos Taurus ◽

Sperm Quality ◽

Membrane Integrity ◽

Plasma Membrane Integrity ◽

Flower Extract ◽

Randomized Design ◽

Bos Taurus Indicus ◽

Fresh Semen

The objective of this research was to investigate the effect of flamboyant flower extract as a natural antioxidant on sperm quality of Aceh cattle after dilution and equilibration. Fresh semen was collected from 2 healthy bull aged 3.5-4 years old. The research took place in BIB Laboratory. The method used was CRD (Completed Randomized Design), with 6 treatments and 5 replications each The treatments (m/v) were 0%, 0.05%, 0.10%, 0.15% and 0.20% of flamboyant flower extracts. The method used was CRD (Completed Randomized Design), with 6 treatments and 5 replications each. The treatments (m/v) were 0%, 0.05%, 0.10%, 0.15% and 0.20% of flamboyant flower extracts. The data obtained were analyzed by analysis of variance (ANOVA). Parameters evaluated in this study were motility, viability, plasma membrane integrity, and abnormality. Results showed that there was an insignificant effect (p>0.05) of flamboyant flower extract on the quality of Aceh cattle spermatozoa. The best percentage of motility and viability, 80.0% and 88.4%, respectively, were found at P3 (0.15% flamboyant flower extract). In can be concluded from the research that the addition of 0.15% (m/v) D. regia flower extract was able to maintain sperm quality of Aceh cattle to be used in the artificial insemination program.

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Comparison between Tris-buffer and INRA-82 extenders on the quality of chilled rabbit spermatozoa

World Rabbit Science ◽

10.4995/wrs.2020.12287 ◽

2020 ◽

Vol 28 (1) ◽

pp. 13

Author(s):

A.M. Fadl ◽

A.M. Ghallab ◽

M.M. Abou-Ahmed

Keyword(s):

Membrane Integrity ◽

Zealand White Rabbit ◽

Tris Buffer ◽

Dna Integrity ◽

Sperm Viability ◽

New Zealand White Rabbit ◽

Progressive Motility ◽

Viability Percentage ◽

Rabbit Spermatozoa

The aim of the present study was to compare the effects of tris-buffer and INRA-82 extenders on the quality of cooled rabbit spermatozoa. Pooled semen samples were collected from eight New Zealand White rabbit bucks, divided into three groups and diluted 1:5 with three different semen extenders: INRA-82, tris-citrate glucose (TCG) and tris-citrate trehalose (TCT). Following dilution, the samples were stored at 5°C for up to 48 h. Sperm motility was recorded at 24, 36 and 48 h post-cooling. Furthermore, sperm viability, morphology, membrane integrity, acrosome status and DNA integrity were assessed at 24 h post-cooling. We found that progressive motility percentages were significantly higher (P<0.05) in samples diluted in INRA-82 (61.00, 53.50, 44.00% at 24, 36 and 48 h post-cooling, respectively) than those diluted in either TCG (39.25, 32.00, 19.75%) or in TCT (47.25, 40.50, 29.00%). We also reported that sperm viability, percentage of normal spermatozoa, percentage of spermatozoa with intact acrosome and DNA integrity after 24 h cooling were significantly higher (P<0.05) in INRA-82 diluted samples compared to TCG and TCT diluted ones. In summary, our results show that dilution of rabbit semen in INRA-82 improved sperm physiological parameters post-cooling compared to those diluted in TCG or TCT. Our findings also suggest that INRA-82 is a promising diluent that can be used effectively to maintain the viability of chilled rabbit semen.

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PENGARUH PENAMBAHAN MADU DENGAN DOSIS BERBEDA DALAM MEDIA PENGENCER NaCl FISIOLOGIS TERHADAP KUALITAS SPERMA IKAN TAWES (Barbonymus gonionotus)

Sains Akuakultur Tropis ◽

10.14710/sat.v3i2.3904 ◽

2019 ◽

Vol 3 (2) ◽

Author(s):

Oni Septiana Devi ◽

Titik Susilowati ◽

Ristiawan Agung Nugroho

Keyword(s):

Sperm Quality ◽

Sperm Concentration ◽

Fertilization Rate ◽

Hatching Rate ◽

Dilute Solution ◽

Randomized Design ◽

Test Fish ◽

Different Doses ◽

Silver Barb

Silver barb (Barbonymus gonionotus) is one of the freshwater fish that is native in Indonesia. One of the problems in hatching of silver barb is because the maturation period of gamete broodstock male and female fish does not happen together. The solution to solving this problem is through the application of reproductive biotechnology in form of sperm storage. High sperm concentration can inhibit spermatozoa activity and affect sperm to find microphils. The addition of honey in dilute solution of physiological NaCl is expected to be able to improve the quality of sperm of silver barb. The aim this research was to know the effect and the best honey dosage in physiological NaCl on sperm quality of silver barb. The test fish that will be used in this research is 1 broodstock male and 1 broodstock female with 1-1,5 years old. In this research, the Completely Randomized Design experimental method is used. This research also using 4 treatments and 3 repetitions. Those treatment were A (0 ml honey + 100 ml physiological NaCl), B (0,2 ml honey + 99,8 ml physiological NaCl), C (0,4 ml honey + 99,6 ml physiological NaCl), D (0,6 ml honey + 99,4 ml physiological NaCl). The result of this research shows, addition of honey with different doses in dilute solution of physiological NaCl gave significant effect (p < 0,05) on fertilization rate and hatching rate of silver barb. The treatment D (0,6 ml honey + 99,4 ml physiological NaCl) gave the best on fertilization rate 90,67% and hatching rate 86,67%.

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Effect of Incubation Time During Sperm Sexing Process on Sperm Quality of Pasundan Bull

Jurnal Ilmu Ternak dan Veteriner ◽

10.14334/jitv.v25i3.2494 ◽

2020 ◽

Vol 25 (3) ◽

pp. 112

Author(s):

Siti Darodjah Rasad ◽

Nurcholidah Solihati ◽

Kikin Winangun ◽

Annisa Yusrina ◽

Fahmy Avicenna

Keyword(s):

Plasma Membrane ◽

Incubation Time ◽

Lower Layer ◽

Sperm Quality ◽

Membrane Integrity ◽

Dna Integrity ◽

Test Parameter ◽

Plasma Membrane Integrity ◽

Randomized Design

The research was conducted to evaluate the effect of incubation time on viability, plasma membrane integrity, abnormality, and DNA integrity of sexed Pasundan’s bulls sperm. The sperm sexing used 5% and 10% concentrations of Bovine Serum Albumin (BSA). A completely randomized design with three treatments and six replications was used in this study. The data were analyzed using variance analysis followed by Duncan’s multiple distance test. Parameter evaluated were sperm longevity, plasma membrane integrity (PMI), abnormality, and DNA integrity of sexed Pasundan bulls sperm. Results showed that incubation time gave significant effect (P<0.05) on the longevity of sperm, but not on the PMI of Pasundan bulls sexed sperm. The incubation time of 45 minutes gave the highest value of longevity sperm on the upper layer (4.33 days) and the lower layer (4.17 days). Furthermore, the abnormality of sperm X in the upper layer was 4.00%-4.20% and the lower layer was 4.10%- 4.40%. Meanwhile, the DNA integrity of an upper layer was 98.16%-98.66%, and the lower layer was 97.83%-98.58%. It is concluded that 45 minutes of incubation time significantly affected the longevity of sperm, but not plasma membrane integrity, abnormality, and DNA integrity of Pasundan bulls sexed sperm.

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Quality of ram spermatozoa separated with modified swim up method

Bangladesh Veterinarian ◽

10.3329/bvet.v33i2.36459 ◽

2018 ◽

Vol 33 (2) ◽

pp. 62-70 ◽

Cited By ~ 2

Author(s):

A Hossain ◽

MM Islam ◽

F Naznin ◽

RN Ferdousi ◽

FY Bari ◽

...

Keyword(s):

Semen Quality ◽

Membrane Integrity ◽

Quality Parameters ◽

Fluid Medium ◽

Normal Morphology ◽

The Mean ◽

Mass Activity ◽

Fresh Semen ◽

Artificial Vagina

Semen was collected from four rams, using artificial vagina and viability%, motility% and plasma membrane integrity% were measured. Fresh ejaculates (n = 32) were separated by modified swim-up separation using modified human tubal fluid medium. Four fractions of supernatant were collected at 15-minute intervals. The mean volume, mass activity, concentration, motility%, viability%, normal morphology and membrane integrity% (HOST +ve) of fresh semen were 1.0 ± 0.14, 4.1 ± 0.1 × 109 spermatozoa/ml, 85.0 ± 1.3, 89.4 ± 1.0, 85.5 ± 0.7, 84.7 ± 0.5 respectively. There was no significant (P>0.05) difference in fresh semen quality parameters between rams. The motility%, viability% and HOST +ve % of first, second, third and fourth fractions were 53.4 ± 0.5, 68.2 ± 0.3, 74.8 ± 0.3 and 65.5 ± 0.4; 55.5 ± 0.4, 66.2 ± 0.4, 74.5 ± 0.3 and 73.6 ± 0.3 and 66.7 ± 0.5, 66.8 ± 0.5, 65.2 ± 0.4 and 74.7 ± 0.5 respectively. The motility%, viability% and membrane integrity% of separated semen samples differed significantly (P<0.05) between four fractions. The mean motility% and viability% were significantly higher (P<0.05) in third fraction (74.8 ± 0.3%), whereas the mean HOST +ve% was significantly higher (P<0.05) in fourth fraction (74.7 ± 0.5). All quality parameters of separated spermatozoa were significantly (P<0.05) lower than that of fresh semen. The pregnancy rates were higher with fresh semen (71%) in comparison to that of separated sample (57%).Bangl. vet. 2016. Vol. 33, No. 2, 62-70

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