scholarly journals ELECTRICAL CONDUCTIVITY AND ETHANOL RELEASE TO ASSESS RED RICE SEED VIGOR1

2021 ◽  
Vol 34 (4) ◽  
pp. 791-798
Author(s):  
RAFAEL MARANI BARBOSA ◽  
MATHEUS ANDRÉ DE JESUS ◽  
RAFAELA ALVES PEREIRA ◽  
GEDEON ALMEIDA GOMES JUNIOR
Keyword(s):  
Electrical Conductivity ◽  
Efficient Method ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Rice Seed ◽  
Seed Sample ◽  
Red Rice ◽  
Germination Test ◽  
Rice Seeds

ABSTRACT To evaluate seed vigor, electrical conductivity and ethanol tests are fast and efficient methodologies. They have the potential to be used in several species, such as red rice. However, there are no protocols or information about their efficiency. Thus, the objective was to evaluate the efficiency, and define parameters of execution for electrical conductivity and ethanol tests, to evaluate the vigor of red rice seeds. The study was conducted using four lots of ‘BRS 901’ red rice, which was subjected to a germination test, as well as first count, accelerated aging, and field seedling emergence tests. The electrical conductivity test was performed with 25, 50, and 100 seeds soaked in 50 mL and 75 mL of water, at 25 °C and 30 °C, for 3, 6, 20, and 24 hours, respectively. The ethanol test was performed with 50 and 100 seeds soaked in a volume of water equivalent to 1.0, 1.5, 2.0, 2.5, and 3.0× the mass of the seed sample. To assess the vigor of red rice seeds, the electrical conductivity test is an efficient method when conducted with 50 seeds soaked in 50 mL of water at 25 °C for 20 hours. Meanwhile, the ethanol test is most effective when performed with 50 seeds, in a volume of water that is 2.5× the mass of the sample, at 40 °C for 24 hours.

scholarly journals Electrical conductivity of the seed soaking solution and soybean seedling emergence

2004 ◽  
Vol 61 (2) ◽  
pp. 164-168 ◽  
Author(s):  
Roberval Daiton Vieira ◽  
Angelo Scappa Neto ◽  
Sonia Regina Mudrovitsch de Bittencourt ◽  
Maristela Panobianco
Keyword(s):  
Electrical Conductivity ◽  
Water Content ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Soybean Seedling ◽  
Seed Soaking ◽  
Laboratory Conditions ◽  
Field And Laboratory Conditions

Vigor of soybean [Glycine max (L.) Merrill] seeds can be evaluated by measuring the electrical conductivity (EC) of the seed soaking solution, which has shown a satisfactory relationship with field seedling emergence, but has not had aproper definition of range yet. This work studies the relationship between EC and soybean seedling emergence both in the field and laboratory conditions, using twenty two seed lots. Seed water content, standard germination and vigor (EC, accelerated aging and cold tests) were evaluated under laboratory conditions using -0.03; -0.20; -0.40 and -0.60 MPa matric potentials, and field seedling emergence was also observed. There was direct relationship between EC and field seedling emergence (FE). Under laboratory conditions, a decreasing relationship was found between EC and FE as water content in the substrate decreased. Relationships between these two parameters were also found when -0.03; -0.20 and -0.40 MPa matric potentials were used. EC tests can be used successfully to evaluate soybean seed vigor and identify lots with higher or lower field emergence potential.

scholarly journals Assessment of physiological potential of stored pea (Pisum sativum L.) seeds

2013 ◽  
Vol 35 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Fábio Mielezrski ◽  
Julio Marcos-Filho
Keyword(s):  
Electrical Conductivity ◽  
Growth Habit ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Storage ◽  
Seed Vigor ◽  
Seedling Length ◽  
Pea Seeds ◽  
Cold Chamber ◽  
Pea Seed

Research on pea seed storage and the identification of efficient seed vigor tests for this species is still insufficient, especially for cultivars that produce wrinkled seeds used for consumption in natura. The objective of this study was to verify the accuracy of tests to assess the physiological potential of pea seed lots stored in different environments. Four seed lots of two cultivars, 'Telefone Alta' (indeterminate growth habit) and 'Itapuã' (determinate growth habit) were stored under different environmental conditions for 8 months: a) laboratory uncontrolled b) dry and cold chamber (10 ºC and 30% RH), c) controlled environment (20 ºC and 70% RH). Seed vigor (accelerated aging, electrical conductivity, seedling length and seedling emergence) was evaluated initially and after four and eight months storage. Results showed that the use of seed lots of high initial physiological potential is fundamental for guaranteeing the desired performance of pea seeds during storage. Wrinkled pea seeds should be stored between harvest and sowing in an environment in which the sum of the relative humidity (%) and temperature (°C) does not exceed 70. Physiological potential of pea seeds should be evaluated by at least two vigor tests; consideration to accelerated aging and electrical conductivity tests is recommended.

scholarly journals Tests for evaluating the physiological quality of pitaya seeds

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano
Keyword(s):  
Electrical Conductivity ◽  
Pearson Correlation ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Water Volume ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

scholarly journals Treatment of rice seeds with thiamethoxam: reflections on physiological performance

2014 ◽  
Vol 36 (4) ◽  
pp. 458-464 ◽  
Author(s):  
Andreia da Silva Almeida ◽  
Cristiane Deuner ◽  
Carolina Terra Borges ◽  
Géri Eduardo Meneghello ◽  
Adilson Jauer ◽  
...  
Keyword(s):  
Defense Mechanisms ◽  
Seed Quality ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Cold Test ◽  
Rice Seed ◽  
Seedling Length ◽  
Rice Seeds ◽  
Growing Conditions ◽  
System Length

Thiamethoxam is a systemic insecticide that is transported within the plant through its cells and can activate various physiological reactions such as protein expression. These proteins interact with defense mechanisms against stress in adverse growing conditions. The objective of this study was to evaluate the effect of thiamethoxam in rice seeds and the potential benefits that it can provide. Two experiments were carried out and, in both, seeds were treated with commercial product containing 350 g of thiamethoxam active ingredient per liter of product, at doses 0, 100, 200, 300 and 400 mL.100 kg-1 of seeds: 1) it was conducted with three lots of IRGA BR 424 cultivar rice seeds, which were submitted to the following laboratory tests: germination, cold test, accelerated aging test, as well as field assessment: total seedling length, root system length, number of panicles and productivity; 2) four lots of IRGA BR 424 cultivar rice seeds, two high and two low-vigor, were subjected to the following tests: germination, cold test and greenhouse seedling emergence test. Thiamethoxam rice seed treatment positively favors the seed quality.

scholarly journals Seedling image analysis and traditional tests to evaluate onion seed vigor

2017 ◽  
Vol 39 (3) ◽  
pp. 216-223 ◽  
Author(s):  
Nayara Roberto Gonçalves ◽  
Silvio Moure Cicero ◽  
Haynna Fernandes Abud
Keyword(s):  
Electrical Conductivity ◽  
Image Analysis ◽  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Seedling Length ◽  
Onion Seed ◽  
Emergence Percentage

Abstract: The aim of this study was to verify the possibility of using computerized seedling image analysis to detect differences in vigor among onion seed lots and to compare this analysis with traditional vigor tests. We used five seed lots of two onion cultivars, Bella Catarina and Bella Vista. The Seed Vigor Imaging System (SVIS®) software was used to process the seedling images and assess seedling length and vigor through seedling uniformity and growth indexes. These determinations were compared to traditional tests used to evaluate onion seed vigor: germination percentage and first germination count, seedling emergence percentage and emergence speed, accelerated aging, and electrical conductivity. Results showed that vigor differences among onion seed lots can be identified using the SVIS® in a manner similar to traditional vigor tests; assessments should be performed at five days after sowing.

scholarly journals Computerized imaging analysis of seedlings for assessment of physiological potential of wheat seeds

2012 ◽  
Vol 34 (4) ◽  
pp. 589-596 ◽  
Author(s):  
Vanessa Neumann Silva ◽  
Francisco Guilhien Gomes Junior ◽  
Silvio Moure Cicero
Keyword(s):  
Electrical Conductivity ◽  
Image Analysis ◽  
Relative Humidity ◽  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Wheat Cultivars ◽  
Imaging Analysis ◽  
Wheat Seeds

Nowadays, image analysis is one of the most modern tools in evaluating physiological potential of seeds. This study aimed at verifying the efficiency of the seedling imaging analysis to assess physiological potential of wheat seeds. The seeds of wheat, cultivars IAC 370 and IAC 380, each of which represented by five different lots, were stored during four months under natural environmental conditions of temperature (T) and relative humidity (RH), in municipality of Piracicaba, Stated of São Paulo, Brazil. For this, bimonthly assessments were performed to quantify moisture content and physiological potential of seeds by means of tests of: germination, first count, accelerated aging, electrical conductivity, seedling emergence, and computerized analysis of seedlings, using the Seed Vigor Imaging System (SVIS®). It has been concluded that the computerized analyses of seedling through growth indexes and vigor, using the SVIS®, is efficient to assess physiological potential of wheat seeds.

scholarly journals Electrical conductivity and soybean seedling emergence

2004 ◽  
Vol 61 (4) ◽  
pp. 386-391 ◽  
Author(s):  
Julio Cesar Fachini Colete ◽  
Roberval Daiton Vieira ◽  
Alek Sandro Dutra
Keyword(s):  
Electrical Conductivity ◽  
Water Potential ◽  
Laboratory Tests ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Before And After ◽  
Glycine Max L ◽  
The Relationship

Evaluation of soybean [Glycine max (L.) Merrill] seeds vigor and its relation with field seedling emergence can provide secure indicatives of seed physiological potential. The present research studied the relationship between the electrical conductivity test and the seedling emergence of soybean in field and laboratory. The field experiment was sown in November 26th, December 3td and 10th, 2000. Laboratory tests were performed using levels of water potential in the substrate (soil) -0.03; -0.2; -0.4; and -0.6 MPa. Fourteen seed lots, seven of 'Embrapa 48' and seven of 'BRS 133' soybean cultivars were used. Laboratory tests comprised seed water content determination, before and after the accelerated aging; germination and vigor tests (accelerated aging and electrical conductivity). Germination and seedling emergence decreased as the substrate water potential was reduced, indicating a relationship among the germination, water potential and seed vigor. For the 'Embrapa 48' higher vigor seed lots, the best performance occurred at -0.6 MPa water potential. The electrical conductivity test may be efficient to evaluate soybean seed vigor and, consequently, the performance potential in the field. However, further studies are necessary to determine the values or the range of values that indicate the seed vigor level and the adequate use of a given seed lot.

scholarly journals Physiological quality of soybean seeds treated with imidacloprid before and after storage

2020 ◽  
Vol 66 (No. 10) ◽  
pp. 513-518
Author(s):  
Renata Cristiane Pereira ◽  
Murilo Fuentes Pelloso ◽  
Larissa Vinis Correia ◽  
Thaisa Cavalieri Matera ◽  
Rayssa Fernanda dos Santos ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Seed Treatment ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Soybean Seeds ◽  
Germination Test ◽  
Soybean Cultivars ◽  
Before And After ◽  
Physiological Quality

This study aimed to evaluate the effect of different slurry volume of imidacloprid insecticide on the physiological potential during the storage of seeds of three soybean cultivars. A completely randomised experimental design was adopted, in a 6 × 3 × 2 factorial scheme, which treatments were six slurry volumes with imidacloprid insecticide (0, 200, 500, 800, 1 100 and 1 400 mL/100 kg of seeds), 3 soybean cultivars (SYN15630, M5947, and NS5959) and 2 storage periods (0 and 30 days after seed treatment), with four replications. The treated seeds were stored in kraft paper bags with controlled temperature and humidity. The physiological potential of the seeds was evaluated by standard germination test, electrical conductivity, accelerated aging, seedling emergence in the sand seedbed, and the field. The germination and vigor of soybean seeds were reduced during storage, especially with the increase in the volumes of the insecticide solution.  

scholarly journals Parameters of the Accelerated Aging Test for the Determination of Tobacco (Nicotiana tabacum L.) Seed Vigor

2020 ◽  
Vol 8 (1) ◽  
pp. 247
Author(s):  
Luis Henrique Konzen ◽  
Daniele Brandstetter Rodrigues ◽  
Alcimar Spindola Mazon ◽  
William Aldrei Guareschi ◽  
Jerffeson Araujo Cavalcante ◽  
...  
Keyword(s):  
Saline Solution ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Exposure Period ◽  
Seed Vigor ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Tobacco Seeds ◽  
Aging Test

Vigor tests are very important to obtain additional information to the standard germination test and can assist in decision-making. The aim of this work was to evaluate accelerated aging test methodologies for the determination of vigor in tobacco seeds. For the study, ten seed lots of tobacco were used. The initial quality of the tobacco seeds was determined through the germination test, first count of germination, germination speed index, seedling emergence at 7, 14 and 21 days after sowing, emergence speed index and the accelerated aging with water performed as proposed by the Association of Official Seed Analysts. After the determination of seed initial quality, the accelerated aging test was studied in the following methods: accelerated aging with water, saturated saline solution (40g NaCl 100mL-1 water) and unsaturated saline solution (11g NaCl 100mL-1 water), submitted to the temperatures of 45 and 41°C, for the exposure periods of 24, 48 and 72 hours. According to the results obtained, the accelerated aging test with water conducted under a temperature of 45°C combined with a 24-hour exposure period is shown as adequate for evaluating the vigor of tobacco seeds.

scholarly journals Tests for evaluating the physiological quality of pitaya seeds

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano
Keyword(s):  
Electrical Conductivity ◽  
Pearson Correlation ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Water Volume ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

<p>Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe <em>Hylocereus undatus </em>fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p &lt; 0.05). The germination test for <em>H. undatus </em>seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.</p>

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