scholarly journals Seedling image analysis and traditional tests to evaluate onion seed vigor

2017 ◽  
Vol 39 (3) ◽  
pp. 216-223 ◽  
Author(s):  
Nayara Roberto Gonçalves ◽  
Silvio Moure Cicero ◽  
Haynna Fernandes Abud
Keyword(s):  
Electrical Conductivity ◽  
Image Analysis ◽  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Seedling Length ◽  
Onion Seed ◽  
Emergence Percentage

Abstract: The aim of this study was to verify the possibility of using computerized seedling image analysis to detect differences in vigor among onion seed lots and to compare this analysis with traditional vigor tests. We used five seed lots of two onion cultivars, Bella Catarina and Bella Vista. The Seed Vigor Imaging System (SVIS®) software was used to process the seedling images and assess seedling length and vigor through seedling uniformity and growth indexes. These determinations were compared to traditional tests used to evaluate onion seed vigor: germination percentage and first germination count, seedling emergence percentage and emergence speed, accelerated aging, and electrical conductivity. Results showed that vigor differences among onion seed lots can be identified using the SVIS® in a manner similar to traditional vigor tests; assessments should be performed at five days after sowing.

scholarly journals Computerized imaging analysis of seedlings for assessment of physiological potential of wheat seeds

2012 ◽  
Vol 34 (4) ◽  
pp. 589-596 ◽  
Author(s):  
Vanessa Neumann Silva ◽  
Francisco Guilhien Gomes Junior ◽  
Silvio Moure Cicero
Keyword(s):  
Electrical Conductivity ◽  
Image Analysis ◽  
Relative Humidity ◽  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Wheat Cultivars ◽  
Imaging Analysis ◽  
Wheat Seeds

Nowadays, image analysis is one of the most modern tools in evaluating physiological potential of seeds. This study aimed at verifying the efficiency of the seedling imaging analysis to assess physiological potential of wheat seeds. The seeds of wheat, cultivars IAC 370 and IAC 380, each of which represented by five different lots, were stored during four months under natural environmental conditions of temperature (T) and relative humidity (RH), in municipality of Piracicaba, Stated of São Paulo, Brazil. For this, bimonthly assessments were performed to quantify moisture content and physiological potential of seeds by means of tests of: germination, first count, accelerated aging, electrical conductivity, seedling emergence, and computerized analysis of seedlings, using the Seed Vigor Imaging System (SVIS®). It has been concluded that the computerized analyses of seedling through growth indexes and vigor, using the SVIS®, is efficient to assess physiological potential of wheat seeds.

scholarly journals Seedling imaging analysis and traditional tests to assess okra seed vigor

2013 ◽  
Vol 35 (4) ◽  
pp. 443-448 ◽  
Author(s):  
Ana Lúcia Pereira Kikuti ◽  
Júlio Marcos-Filho
Keyword(s):  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Control Programs ◽  
Seed Industry ◽  
Accelerated Aging Test ◽  
Vigor Index ◽  
Seedling Length ◽  
Germination Speed

Seed vigor testing is an important component of quality control programs adopted by seed industry. The software Seed Vigor Imaging System (SVIS) has been successfully used for seed vigor assessment in different species. The objective of this research was to verify the SVIS efficiency to assess okra seed vigor in comparison to other vigor tests used for this species. Five seed lots of 'Clemson Americano' and four of 'Santa Cruz' were submitted to germination (speed and percentage), cold germination (speed and percentage), traditional and saturated salt accelerated aging (41 ºC/72 and 96 h) and seedling emergence tests during 12 months storage. Vigor index, uniformity of growth and seedling length were determined by the software Seed Vigor Imaging System (SVIS). Results showed that the vigor index and seedling length determined by the SVIS analyses are efficient to determine okra seed vigor as well as the accelerated aging test (41 ºC/72 h).

scholarly journals Assessment of physiological potential of stored pea (Pisum sativum L.) seeds

2013 ◽  
Vol 35 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Fábio Mielezrski ◽  
Julio Marcos-Filho
Keyword(s):  
Electrical Conductivity ◽  
Growth Habit ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Storage ◽  
Seed Vigor ◽  
Seedling Length ◽  
Pea Seeds ◽  
Cold Chamber ◽  
Pea Seed

Research on pea seed storage and the identification of efficient seed vigor tests for this species is still insufficient, especially for cultivars that produce wrinkled seeds used for consumption in natura. The objective of this study was to verify the accuracy of tests to assess the physiological potential of pea seed lots stored in different environments. Four seed lots of two cultivars, 'Telefone Alta' (indeterminate growth habit) and 'Itapuã' (determinate growth habit) were stored under different environmental conditions for 8 months: a) laboratory uncontrolled b) dry and cold chamber (10 ºC and 30% RH), c) controlled environment (20 ºC and 70% RH). Seed vigor (accelerated aging, electrical conductivity, seedling length and seedling emergence) was evaluated initially and after four and eight months storage. Results showed that the use of seed lots of high initial physiological potential is fundamental for guaranteeing the desired performance of pea seeds during storage. Wrinkled pea seeds should be stored between harvest and sowing in an environment in which the sum of the relative humidity (%) and temperature (°C) does not exceed 70. Physiological potential of pea seeds should be evaluated by at least two vigor tests; consideration to accelerated aging and electrical conductivity tests is recommended.

scholarly journals Assessment of physiological potential of cucumber seeds using the software Seedling Vigor Imaging System® (SVIS®)

2012 ◽  
Vol 34 (2) ◽  
pp. 255-263 ◽  
Author(s):  
Adrielle Aparecida Chiquito ◽  
Francisco Guilhien Gomes Junior ◽  
Julio Marcos-Filho
Keyword(s):  
Seedling Growth ◽  
Imaging System ◽  
Seedling Emergence ◽  
Primary Root ◽  
Accelerated Aging ◽  
Objective Evaluation ◽  
Seed Vigor ◽  
Cucumis Sativus L ◽  
Seedling Length ◽  
Definition Of

The software Seed Vigor Imaging System (SVIS®), has been successfully used to evaluate seed physiological potential by automated analyses of scanned seedlings. In this research, the efficiency of this system was compared to other tests accepted for assessing cucumber (Cucumis sativus L.) seed vigor of distinct seed lots of Supremo and Safira cultivars. Seeds were subjected to germination, traditional and saturated salt accelerated aging, seedling emergence, seedling length and SVIS analyses (determination of vigor indices and seedling growth uniformity, lengths of primary root, hypocotyl and whole seedlings). It was also determined whether the definition of seedling growth/uniformity ratios affects the sensitivity of the SVIS®. Results showed that analyses SVIS have provided consistent identification of seed lots performance, and have produced information comparable to those from recommended seed vigor tests, thus demonstrating a suitable sensitivity for a rapid and objective evaluation of physiological potential of cucumber seeds. Analyses of four-days-old cucumber seedlings using the SVIS® are more accurate and growth/uniformity does not affect the precision of results.

scholarly journals Computerized image analysis of seedlings to evaluate broccoli seed vigor

2017 ◽  
Vol 39 (3) ◽  
pp. 303-310 ◽  
Author(s):  
Haynna Fernandes Abud ◽  
Silvio Moure Cicero ◽  
Francisco Guilhien Gomes Junior
Keyword(s):  
Image Analysis ◽  
Imaging System ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Computer Image Analysis ◽  
Computerized Image Analysis ◽  
Randomized Design ◽  
Completely Randomized Design

Abstract: The computerized image analysis of seedlings constitutes an efficient technique to evaluate the quality of seed lots, since it is quick in responding, has a simple execution and reproduction. The goal of this research was to verify the efficiency of the computer image analysis of seedlings to detect vigor differences among broccoli seed lots and compare them with other traditionally used vigor tests. Computerized image analysis were performed by the Seed Vigor Imaging System (SVIS®), using 3-day-old seedlings and the results were compared to germination at first count, seedling emergence, accelerated aging and electrical conductivity tests. Evaluations were performed in three experimental periods (before storage, 6 and 12 months of storage at 20 °C and 45-50% RH). A completely randomized design with eight replications of 25 seeds was used. Data were submitted to analysis of variance and means were compared by Scott-Knott test at 5% error probability. It is concluded that it is possible to detect vigor differences among broccoli seed lots by the computerized image analysis of seedlings with SVIS®, similarly to traditional vigor tests.

scholarly journals Electrical conductivity of the seed soaking solution and soybean seedling emergence

2004 ◽  
Vol 61 (2) ◽  
pp. 164-168 ◽  
Author(s):  
Roberval Daiton Vieira ◽  
Angelo Scappa Neto ◽  
Sonia Regina Mudrovitsch de Bittencourt ◽  
Maristela Panobianco
Keyword(s):  
Electrical Conductivity ◽  
Water Content ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Soybean Seedling ◽  
Seed Soaking ◽  
Laboratory Conditions ◽  
Field And Laboratory Conditions

Vigor of soybean [Glycine max (L.) Merrill] seeds can be evaluated by measuring the electrical conductivity (EC) of the seed soaking solution, which has shown a satisfactory relationship with field seedling emergence, but has not had aproper definition of range yet. This work studies the relationship between EC and soybean seedling emergence both in the field and laboratory conditions, using twenty two seed lots. Seed water content, standard germination and vigor (EC, accelerated aging and cold tests) were evaluated under laboratory conditions using -0.03; -0.20; -0.40 and -0.60 MPa matric potentials, and field seedling emergence was also observed. There was direct relationship between EC and field seedling emergence (FE). Under laboratory conditions, a decreasing relationship was found between EC and FE as water content in the substrate decreased. Relationships between these two parameters were also found when -0.03; -0.20 and -0.40 MPa matric potentials were used. EC tests can be used successfully to evaluate soybean seed vigor and identify lots with higher or lower field emergence potential.

scholarly journals Effect of seed vigor and sowing densities on the yield and physiological potential of wheat seeds

2021 ◽  
Vol 43 ◽  
Author(s):  
Carolina Pereira Cardoso ◽  
José Henrique Bizzarri Bazzo ◽  
Jéssica de Lucena Marinho ◽  
Claudemir Zucareli
Keyword(s):  
Seed Yield ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Wheat Crop ◽  
Seedling Length ◽  
Physiological Quality ◽  
Initial Seed ◽  
Wheat Seeds

Abstract: Initial seed vigor and sowing density interact in establishment of plants in the field, and can thus affect expression of the plasticity of the wheat crop and the yield and physiological quality of the seeds produced. The aim of this study was to evaluate the effect of seed vigor levels in combination with sowing densities on the yield and physiological potential of wheat seeds. The cultivars BRS Gralha-Azul and BRS Sabiá were used in a randomized block experimental design in a 2 × 4 factorial arrangement, with four replications. The treatments consisted of seeds of high and low vigor and four sowing densities (150, 250, 350, and 450 seeds.m-2). The following evaluations were made: seed yield, germination, first germination count, seedling length, seedling dry matter, accelerated aging, emergence speed index, and seedling emergence in sand. An increase in sowing density favors the seed yield of both cultivars; however, it reduces the vigor of the seeds produced by the cultivar BRS Gralha-Azul, especially of the seeds produced by plants originating from high vigor seeds. For the cultivar BRS Sabiá, an increase in sowing density decreases the germination performance of seeds produced by plants originating from low vigor seeds.

scholarly journals Correlation between vigor by accelerated aging at pre-sowing and soybean seedling emergence in the field

2021 ◽  
Vol 42 (2) ◽  
pp. 455-470
Author(s):  
Vanderléia Mathias ◽  
◽  
Cileide Maria Medeiros Coelho ◽  
Keyword(s):  
Exposure Time ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Growing Seasons ◽  
Accelerated Aging Test ◽  
Physiological Quality ◽  
Field Emergence

Although the accelerated aging test is widely used to determine seed vigor, there are methodological variations in seed exposure time at 41 °C. Therefore, the objective was to indicate the most appropriate seed exposure time to segregate lots that also exhibits a correlation with seedling emergence in the field, and establish vigor levels using the aforementioned test. Soybean seed samples were collected at pre-sowing, representing 100 lots in the 2016/2017 growing season and 125 lots in the 2017/2018 season from different warehouses in 4 regions of Santa Catarina state (SC). Physiological quality was evaluated by germination, accelerated aging (24 and 48 h at 41 °C) and field emergence tests. The results were submitted to descriptive statistical analysis and Pearson’s correlation analysis (p < 0.05). The rank sum index, associated with the Scott-Knott clustering method, was used to classify physiological quality. The average germination percentage was 90 and 91% in the 2016/2017 and 2017/2018 growing seasons, respectively. Vigor obtained an average of 86 (2016/2017) and 85% (2017/2018), when seeds were submitted to accelerated aging for 48 h, and 89% (both seasons) for 24 h. In regard to field emergence, the average was 85 and 80% in the 2016/2017 and 2017/2018 growing seasons, respectively. Considering the 225 lots, the greatest correlation with field emergence was observed in vigor - 48 h (r = 0.71 p < 0.05). Rank sum classification made it possible to establish vigor-based categories, whereby batches with over 85% vigor by 48-h accelerated aging were classified as high vigor, 70 to 84% medium vigor, and under 70% low vigor.

scholarly journals ELECTRICAL CONDUCTIVITY AND ETHANOL RELEASE TO ASSESS RED RICE SEED VIGOR1

2021 ◽  
Vol 34 (4) ◽  
pp. 791-798
Author(s):  
RAFAEL MARANI BARBOSA ◽  
MATHEUS ANDRÉ DE JESUS ◽  
RAFAELA ALVES PEREIRA ◽  
GEDEON ALMEIDA GOMES JUNIOR
Keyword(s):  
Electrical Conductivity ◽  
Efficient Method ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Rice Seed ◽  
Seed Sample ◽  
Red Rice ◽  
Germination Test ◽  
Rice Seeds

ABSTRACT To evaluate seed vigor, electrical conductivity and ethanol tests are fast and efficient methodologies. They have the potential to be used in several species, such as red rice. However, there are no protocols or information about their efficiency. Thus, the objective was to evaluate the efficiency, and define parameters of execution for electrical conductivity and ethanol tests, to evaluate the vigor of red rice seeds. The study was conducted using four lots of ‘BRS 901’ red rice, which was subjected to a germination test, as well as first count, accelerated aging, and field seedling emergence tests. The electrical conductivity test was performed with 25, 50, and 100 seeds soaked in 50 mL and 75 mL of water, at 25 °C and 30 °C, for 3, 6, 20, and 24 hours, respectively. The ethanol test was performed with 50 and 100 seeds soaked in a volume of water equivalent to 1.0, 1.5, 2.0, 2.5, and 3.0× the mass of the seed sample. To assess the vigor of red rice seeds, the electrical conductivity test is an efficient method when conducted with 50 seeds soaked in 50 mL of water at 25 °C for 20 hours. Meanwhile, the ethanol test is most effective when performed with 50 seeds, in a volume of water that is 2.5× the mass of the sample, at 40 °C for 24 hours.

scholarly journals PRODUTIVIDADE E QUALIDADE FISIOLÓGICA DE SEMENTES DE CULTIVARES DE TRIGO EM DIFERENTES DENSIDADES DE SEMEADURA

2018 ◽  
Vol 14 (3) ◽  
pp. 122-131
Author(s):  
Jéssica de Lucena Marinho ◽  
José Henrique Bizzarri Bazzo ◽  
Carolina Pereira Cardoso ◽  
Claudemir Zucareli ◽  
Inês Cristina de Batista Fonseca
Keyword(s):  
Agricultural Research ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Dry Weight ◽  
Seed Vigor ◽  
Seeding Density ◽  
Seed Productivity ◽  
Seedling Length ◽  
Physiological Quality

Determination of optimum wheat sowing density, adequate to the genotype and the environment, is essential to avoid lodging and competition between plants, which can reduce seed productivity and quality. In this sense, the objective of this study was to evaluate the effect of seeding density (in cultivars BRS Sabiá e BRS Gralha-Azul) on productivity and physiological quality of seeds of two wheat cultivars. The seeds were produced in the experimental field of the Brazilian Agricultural Research Corporation, in Londrina-PR, at the National Center for Soy Research (Embrapa Soja) in experimental design of randomized blocks in a 2x4 factorial scheme, with four replications. The following evaluations were performed: seed productivity, thousand seeds mass, first count of germination, germination, seedling length, seedling dry weight, accelerated aging, seedling emergence in sand and emergency speed index. In general, the increase in seed density increases seed production and does not alter germination and emergence of seedlings of both cultivars. The cultivar BRS Gralha-Azul proved to be more productive than BRS Sabiá. The increase in seeding density increases seed vigor for BRS Sabiá cultivar and reduces to BRS Gralha-Azul cultivar.

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