scholarly journals Development of microsatellite loci for Cryptocarya mandioccana Meisner (Lauraceae) and their genotyping success in different tissues

Hoehnea ◽  
2017 ◽  
Vol 44 (4) ◽  
pp. 580-587
Author(s):  
Thabata Carvalho ◽  
Giuliana Garcia ◽  
Carolina Silva Carvalho ◽  
Clarisse Palma-Silva ◽  
Laurence Culot
Keyword(s):  
Genetic Structure ◽  
Microsatellite Loci ◽  
Spatial Genetic Structure ◽  
Atlantic Rainforest ◽  
Adult Trees ◽  
Polymorphic Microsatellite ◽  
Large Animals ◽  
Two Populations ◽  
Different Tissues

ABSTRACT Nine polymorphic microsatellite loci were isolated and characterized for Cryptocarya mandioccana Meisner, a tree from the Atlantic Rainforest with seeds dispersed by large animals. The loci were characterized using 48 individuals from two populations and their genotyping success tested in four tissues: leaves from adults and seedlings, and two diaspore maternal tissues. Maternity analyses were also performed on diaspores and leaves from nine adult trees. The number of alleles per locus ranged from nine to 15 and the observed and expected heterozygosities ranged from 0.214 to 0.864 and 0.745 to 0.892, respectively. The loci genotyping success did not significantly differ between tissues and varied from 56 to 96%. The microsatellites showed enough polymorphism to assign the nine adult trees to their diaspores. The successful genotyping in all tissues and identification of mother trees show that the microsatellites are suitable for studies such as spatial genetic structure and maternity analyses.

Population genetic structure of wild and farmed rusa deer (Cervus timorensis russa) in New-Caledonia inferred from polymorphic microsatellite loci

Genetica ◽  
2009 ◽  
Vol 137 (3) ◽  
pp. 313-323 ◽  
Author(s):  
M. de Garine-Wichatitsky ◽  
T. de Meeûs ◽  
C. Chevillon ◽  
D. Berthier ◽  
N. Barré ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Population Genetic Structure ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
New Caledonia ◽  
Polymorphic Microsatellite

Genetic differences within natural and planted stands of Quercus petraea

2011 ◽  
Vol 6 (4) ◽  
pp. 597-605 ◽  
Author(s):  
Jiří Dostálek ◽  
Tomáš Frantík ◽  
Miroslava Lukášová
Keyword(s):  
Genetic Structure ◽  
Genetic Differentiation ◽  
Spatial Genetic Structure ◽  
Quercus Petraea ◽  
Genetic Differences ◽  
Sessile Oak ◽  
Population Structures ◽  
Nuclear Microsatellite ◽  
The Impact ◽  
Two Populations

AbstractFive sessile oak [Quercus petraea (Matt.) Liebl.] stands from the Czech Republic were studied to learn about the impact of different types of forest management regimes on the genetic differences among tree populations and on population structures. One population had not been markedly affected by human activity, two populations represented unplanted stands that were extensively managed for a long period of time using the coppice system, and two populations were planted stands. Approximately 100 trees from each stand were mapped and subsequently genotyped using 10 nuclear microsatellite loci. We determined the spatial genetic structure of each population and the genetic differentiation among the populations. We found that: (i) the populations were genetically differentiated, but the differences between the unplanted and planted stands were not markedly significant; (ii) the genetic differentiation among the populations depended on the geographical distribution of the populations; (iii) within unplanted stands, a strong spatial genetic structure was seen; and (iv) within planted stands, no spatial genetic structure was observed. Our findings implies that the analysis of spatial genetic structure of the sessile oak forest stand can help reveal and determine its origin.

scholarly journals Spatial Genetic Structure Within Two Contrasting Stands of Scots Pine (Pinus sylvestris L.)

2008 ◽  
Vol 57 (1-6) ◽  
pp. 193-202 ◽  
Author(s):  
I. J. Chybicki ◽  
A. Dzialuk ◽  
M. Trojankiewicz ◽  
M. Slawski ◽  
J. Burczyk
Keyword(s):  
Genetic Variation ◽  
Gene Flow ◽  
Genetic Structure ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Spatial Genetic Structure ◽  
Nuclear Markers ◽  
Genetic Pool ◽  
Establishment History ◽  
Two Populations

AbstractWhen considering neutral nuclear markers, genetic differentiation of Scots pine (Pinus sylvestris L.) populations is known to be low. The homogeneity arises particularly as an effect of common ancestry in a recent evolutionary history as well as an extensive gene flow, especially through pollen. However, within populations several other forces may shape the spatial distribution of genetic variation, including establishment history, environmental and silvicultural selection. These local forces are known to produce non-random spatial patterns of genetic variation, however little is known on fine-scale spatial genetic structure of Scots pine. In this study, two stands of this species with different establishment histories, selected within one larger population located in northern Poland were genotyped and analysed for genetic variation and within-stand spatial genetic structure. Results revealed no differences in genetic variation, although stands are separated about 60 km, suggesting that the two populations share a common genetic pool. The spatial genetic structure in both stands was found to be slightly different and was attributed to differences in the mode of populations’ establishments. Finally, results confirmed that gene flow in Scots pine is extensive, causing genetic homogeneity within a single population.

scholarly journals Genetic diversity and spatial genetic structure in populations of Orbignya phalerata Mart. under different exploitation intensities in the Brazilian savanna

2015 ◽  
Vol 64 (1-6) ◽  
pp. 201-211 ◽  
Author(s):  
Bruna Ibanes ◽  
A.M. Sebbenn ◽  
V.C.R. Azevedo ◽  
M.A. Moreno ◽  
F.B. Gandara ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Spatial Genetic Structure ◽  
Spatial Distance ◽  
Null Alleles ◽  
Fixation Index ◽  
Genetic Studies ◽  
Expected Heterozygosity ◽  
Adult Trees ◽  
Diversity Structure

Abstract Genetic studies in tropical tree species have found signs of decreased genetic diversity and increased levels of inbreeding and spatial genetic structure (SGS) in fragmented and exploited populations. The aim of this paper was to investigate genetic diversity, structure, and intrapopulation SGS using eight microsatellite loci for three Orbignya phalerata populations that have undergone different intensities of seed harvesting. From each population, we georeferenced and sampled 30 seedlings, 30 juveniles, and 30 adult trees. The total number of alleles over all loci (k), and observed (Ho) and expected heterozygosity (He) presented lower values for the population experiencing more intense fruit harvesting than less heavily exploited populations, suggesting that fruit harvesting may decrease genetic diversity. Null alleles were detected in practically all loci among seedlings, juveniles, and adults in all populations, indicating that the estimates of Ho, He, and fixation index (F) are biased. When corrected for null alleles (FNull), the fixation index decreased for all samples, resulting in significantly higher than zero results for seedlings of all populations, but not for juveniles and adults of all populations. The comparison of FNull values between cohorts in the most heavily exploited population (ESP) suggests that inbred individuals are eliminated between seedling and adult stages. Significant SGS was detected up to 60 m in all populations, which indicates short distance seed dispersal. Genetic differentiation (G’ST) between pairwise populations was related to spatial distance between populations, with the greatest difference between more distant populations.

Population structure and parentage analysis of black poplar along the Morava River

2006 ◽  
Vol 36 (5) ◽  
pp. 1067-1076 ◽  
Author(s):  
Markéta Pospíšková ◽  
Ivana Šálková
Keyword(s):  
Genetic Structure ◽  
Populus Deltoides ◽  
Spatial Genetic Structure ◽  
Glacial Refugia ◽  
Parentage Analysis ◽  
Hybrid Poplars ◽  
Adult Trees ◽  
Nuclear Microsatellite ◽  
Dispersal Distances ◽  
Morava River

Twelve nuclear microsatellite markers were used to assess the population genetic structure of the riparian pioneer tree species Populus nigra L. along the Morava River in the Czech Republic. Parentage analysis of 30 seedlings was performed to examine the extent of hybridization between P. nigra and introduced hybrid poplars and to determine the distances of pollen and seed movement. Additionally, spatial genetic structure was analysed and gene dispersal was estimated indirectly. In spite of the limited size of our studied population (65 adult trees), the apparent overall genetic diversity was high (expected heterozygosity He = 0.82) and comparable to the known diversity of P. nigra in southern Europe, where its glacial refugia were located. Introgression of Populus deltoides Bartr. ex Marsh. genes to P. nigra was confirmed, since 13% of tested seedlings descended from a Populus ×canadensis Moench female. The results of parentage analysis showed that a low percentage (20%) of offspring originated from parents located outside the study site. Dispersal distances for pollen and seeds movement ranged from 10 to 230 m and from 163 to 370 m, respectively. The study revealed significant spatial genetic structure (regression slope –0.0158), which was probably caused by limited gene flow.

scholarly journals Gene flow in an overexploited population of Swietenia macrophylla King (Meliaceae) in the Bolivian Amazon

2012 ◽  
Vol 61 (1-6) ◽  
pp. 212-220 ◽  
Author(s):  
A. M. Sebbenn ◽  
J. C. Licona ◽  
B. Mostacedo ◽  
B. Degen
Keyword(s):  
Genetic Structure ◽  
Spatial Genetic Structure ◽  
Pollen Dispersal ◽  
Dispersal Distance ◽  
Fixation Index ◽  
Permanent Plots ◽  
Swietenia Macrophylla ◽  
Genetic Structure Of Populations ◽  
Bolivian Amazon ◽  
Adult Trees

Abstract Pollen and seed movement among and within populations connect individuals and populations, and therefore are among the most important evolutionary processes determining the genetic structure of populations. Seven microsatellite loci were used to investigate the realized pollen dispersal and intra-population spatial genetic structure (SGS) in four permanent plots located in an overexploited big-leaf mahogany (Swietenia macrophylla King) population in the Bolivian Amazon. All adult trees found in the plots were mapped, sampled and genotyped. Seedlings were sampled below the canopy of reproductive trees. Private alleles in the sub-population of the adults and the seedlings were observed. The observed heterozygosity was significantly lower and fixation index was significantly higher for the seedlings (Ho=0.697, F=0.068) compared to the adults (Ho=0.761, F=-0.023). In one plot, seed immigration was observed (18%). Realized pollen immigration ranged among the plots from zero to 41% and selfing ranged from zero to 5.8%. We observed an average pollen dispersal distance from 75 to 255 m, with the maximum reaching 576 m. We found a significant SGS up to 150 m, showing that near neighbour individuals are relatives. The observed data on pollen- and seed dispersal provides important information for the sustainable management of the endangered mahogany species.

scholarly journals Isolation and Characterization of 10 Polymorphic Microsatellite Loci in Paphiopedilum concolor (Batem.) Pfitzer (Orchidaceae) and Cross-species Amplification

HortScience ◽  
2010 ◽  
Vol 45 (8) ◽  
pp. 1286-1287 ◽  
Author(s):  
Long-na Li ◽  
Songjun Zeng ◽  
Feng Zheng ◽  
Zhi-lin Chen ◽  
Kun-lin Wu ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Microsatellite Locus ◽  
Microsatellite Loci ◽  
Genomic Library ◽  
Enriched Genomic Library ◽  
Relationship Patterns ◽  
Isolation And Characterization ◽  
Expected Heterozygosity ◽  
Polymorphic Microsatellite

Ten polymorphic microsatellite loci were isolated and characterized from an enriched genomic library of Paphiopedilum concolor (Batem.) Pfitzer. The number of alleles per microsatellite locus ranged from three to 11 with an average of 6.4 in a sample of 30 individuals from three populations. The observed and expected heterozygosity ranged from 0.200 to 0.800 and from 0.544 to 0.827, respectively. These microsatellites can be used as tools to investigate the genetic structure of P. concolor populations and relationship patterns with closely related taxa.

scholarly journals Genetic diversity and structure of Astrocaryum jauari (Mart.) palm in two Amazon river basins

2014 ◽  
Vol 14 (3) ◽  
pp. 166-173 ◽  
Author(s):  
Liliane D. Santos Oliveira ◽  
Santiago L. Ferreyra Ramos ◽  
Maria T. Gomes Lopes ◽  
Gabriel Dequigiovanni ◽  
Elizabeth Ann Veasey ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Structure ◽  
Microsatellite Loci ◽  
Plant Material ◽  
Spatial Genetic Structure ◽  
River Basins ◽  
Genetic Analyses ◽  
Main River ◽  
Genetic Diversity And Structure

Astrocaryum jauari is a non-domesticated palm that is exploited by poachers. Our objective was to investigate the organization of the geneticdiversity and structure of three A. jauari populations. The study was carried out in the state of Amazonas, between the municipalities of Coari and Manaus. Nine microsatellite loci were used for the genetic analyses. High genetic variation was found, with a mean number of alleles per locus varying from 3.9 to 4.4. The average observed heterozygosity, varying from 0.71 to 0.78, was higher than expected. No spatial genetic structure was detected, since only one cluster was observed. Our results indicate a possible dispersion strategy and suggest that conservation measures of this species should focus mainly on the populations found at the end of the main river (Solimões) where most of the plant material originating from the headwaters of the tributaries of this river is concentrated.

Sign in / Sign up

Export Citation Format

Share Document