scholarly journals Lethal effect of a bait for Rhodnius prolixus (Hemiptera: Reduviidae), the vector of Chagas' disease, containing hexachlorocyclohexane (HCH), under laboratory conditions

1992 ◽  
Vol 34 (4) ◽  
pp. 295-301 ◽  
Author(s):  
Marli Maria Lima ◽  
Luis Rey ◽  
Rubens Pinto de Mello
Keyword(s):  
Mortality Rate ◽  
Chagas Disease ◽  
Room Temperature ◽  
Lethal Effect ◽  
Rhodnius Prolixus ◽  
Active Principle ◽  
Laboratory Conditions

The lethal effect of a bait containing an aqueous hexachlorocyclohexane (HCH) suspension at the concentration of 1g/l and maintained at room temperature was studied in the laboratory over a period of 12 weeks. The suspension was placed in a latex bag hanging inside a 1000-ml beaker tightly covered with nylon netting, and left there with no changes for 85 days. Sixteen groups of R. prolixas bugs, consisting on average of 30 specimens each, were successively exposed to the bait and observed at different intervals for one week each. The mortality rate was 100% for all groups, except for the 16th, whose mortality rate was 96.7%. As the groups succeeded one another, mortality started to occur more rapidly and was more marked at the 6- and 24-h intervals. Later tests respectively started at 6:00 a.m. and 6:00 p.m. showed that diurnal and nocturnal periodicity in the offer of food had no effect on mortality. First- and 2nd- instar nymphs and adults male were more sensitive and 5th- instar nymphs were more resistant to the active principle of the bait.

scholarly journals Lethality of triatomines (Hemiptera: Reduviiae), vectors of Chagas' disease, feeding on blood baits containing synthetic insecticides, under laboratory conditions

1991 ◽  
Vol 33 (6) ◽  
pp. 427-433 ◽  
Author(s):  
Marli Maria Lima ◽  
Luis Rey ◽  
Rubens Pinto de Mello
Keyword(s):  
Chagas Disease ◽  
Laboratory Study ◽  
Room Temperature ◽  
Oral Route ◽  
Triatoma Infestans ◽  
Active Principle ◽  
Defibrinated Sheep Blood ◽  
Panstrongylus Megistus ◽  
Killing Rate ◽  
Triatomine Species

A laboratory study was conducted to test the toxicity of synthetic insecticides added to defibrinated sheep blood kept at room temperature and offered as food to the following triatomine species: Triatoma infestans, Panstrongylus megistus, Triatoma vitticeps, Triatoma pseudomaculata, Triatoma brasiliensis and Rhodnius prolixus. The insecticides used, at a concentration of 1g/l, were: HCH, DDT, Malathion and Trichlorfon, and the lethalithy observed at the end of a 7-day period varied according to the active principle of each. HCH was the most effective by the oral route, killing 100% of the insects, except P. megistus (95.7%) and T. pseudomaculata (94.1%). Trichlorfon killed the insects at rates ranging from 71.8% (T. vitticeps) to 98% (R. prolixus). Malathion was slightly less efficient, killing the insects at rates from 56.8% (T. vitticeps) to 97% (T.brasiliensis). DDT was the least effective, with a killing rate of 10% (T. vitticeps) to 75% (T.brasiliensis). Since the tests were performed at room temperature, we suggest that baits of this type should be tried for the control of triatomines in the field.

scholarly journals Silencing the odorant receptor co-receptor RproOrco affects the physiology and behavior of the Chagas disease vector Rhodnius prolixus

2016 ◽  
Vol 69 ◽  
pp. 82-90 ◽  
Author(s):  
Thiago A. Franco ◽  
Daniele S. Oliveira ◽  
Monica F. Moreira ◽  
Walter S. Leal ◽  
Ana C.A. Melo
Keyword(s):  
Chagas Disease ◽  
Odorant Receptor ◽  
Rhodnius Prolixus ◽  
Disease Vector ◽  
And Behavior

scholarly journals Evaluation of temporal, seasonal and geographic stability of the molluscicidal property of Euphorbia splendens latex

1992 ◽  
Vol 34 (3) ◽  
pp. 183-191 ◽  
Author(s):  
Virginia Torres Schall ◽  
Mauricio Carvalho de Vasconcellos ◽  
Ana Luiza Villaça-Coelho ◽  
Fátima Eliana Ferreira-Lopes ◽  
Ivonise Paz da Silva
Keyword(s):  
Aqueous Solutions ◽  
Laboratory Tests ◽  
Large Scale ◽  
Room Temperature ◽  
Tropical Climate ◽  
Wide Distribution ◽  
Promising Material ◽  
Active Principle ◽  
Reference Substance ◽  
Belo Horizonte

Laboratory tests with aqueous solutions of Euphorbia splendens var. hislopii latex have demonstrated seasonal stability of the molluscicidal principle, with LD90 values of 1.14 ppm (spring), 1.02 ppm (fall), 1.09 ppm (winter), and 1.07 ppm (summer) that have been determined against Biomphalaria tenagophila in the field. Assays on latex collected in Belo Horizonte and Recife yielded LD90 values similar to those obtained with the reference substance collected in Rio de Janeiro (Ilha do Governador), demonstrating geographic stability of the molluscicidal effect. The molluscicidal action of aqueous dilutions of the latex in natura, centrifuged (precipitate) and lyophilized, was stable for up to 124 days at room temperature (in natura) and for up to 736 days in a common refrigerator at 10 to 12ºC (lyophilized product). A 5.0 ppm solution is 100% lethal for snails up to 13 days after preparation, the effect being gradually lost to almost total inactivity by the 30th day. This observation indicated that the active principle is instable. These properties together with the wide distribution of the plant, its resistance and adaptation to the tropical climate, its easy cultivation and the easy obtention of latex and preparation of the molluscicidal solution, make this a promising material for large-scale use in the control of schistosomiasis

Transformation of Rhodococcus rhodnii, a symbiont of the Chagas disease vector Rhodnius prolixus, with integrative elements of the L1 mycobacteriophage

2003 ◽  
Vol 3 (2) ◽  
pp. 103-109 ◽  
Author(s):  
Ellen M Dotson ◽  
Bonnie Plikaytis ◽  
Thomas M Shinnick ◽  
Ravi V Durvasula ◽  
Charles B Beard
Keyword(s):  
Chagas Disease ◽  
Rhodnius Prolixus ◽  
Disease Vector

scholarly journals Growth of immature Chironomus calligraphus (Diptera, Chironomidae) in laboratory conditions

2011 ◽  
Vol 83 (4) ◽  
pp. 1279-1286 ◽  
Author(s):  
Rita de Cássia S.A. Canteiro ◽  
Edélti F. Albertoni
Keyword(s):  
Native Species ◽  
Room Temperature ◽  
Average Length ◽  
Larval Growth ◽  
Time Duration ◽  
Constant Growth Rate ◽  
Fish Food ◽  
Laboratory Conditions ◽  
Spiral Form ◽  
Constant Growth

Chironomidae larvae are important macroinvertebrates in limnic environments, but little knowledge exists about their biometrics development characteristics. This study aims to describe the immature Chironomus calligraphus Goeldi, 1905 under laboratory conditions by the accomplishment of thirteen egg masses from eggs eclosion to adults emergency, at controlled room temperature (25ºC) and photoperiod (12-12h). Larvae were feed ad libitum with "Alcon Basic - MEP 200 Complex" fish food and commercial dehydrated Spirulina. The postures had a mean length of 9 ± 1 mm (n = 13) and 348 ± 66 eggs. The brownish colored eggs with elliptical shape had length of 160.3 ± 17.7 µm (n = 130), being arranged as an organized string in a pseudo spiral form. The time duration from the first to the four instars were three, four, four and eight days, and the average length of a cephalic capsule to each one of the instars (66.3 ± 12.3 µm, 102.9 ± 22.1 µm, 159 ± 24.6 µm, 249.2 ± 29.7 µm, n = 456) were significantly different (ANOVA, p < 0.001). The Dyar’s Rule showed a constant growth rate, r = 1.5. Our results demonstrated that C. calligraphus is a species with short life cycle, low mortality rate, food adaptability, fast larval growth and easily maintained at laboratory, factors that allowed the use of this native species as a tool for ecotoxicological tests.

scholarly journals High-Throughput Identification of the Rhodnius prolixus Midgut Proteome Unravels a Sophisticated Hematophagic Machinery

Proteomes ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 16 ◽  
Author(s):  
Radouane Ouali ◽  
Karen Caroline Valentim de Brito ◽  
Didier Salmon ◽  
Sabrina Bousbata
Keyword(s):  
Digestive Tract ◽  
Chagas Disease ◽  
High Throughput ◽  
Control Strategies ◽  
Anterior Part ◽  
Rhodnius Prolixus ◽  
Etiologic Agent ◽  
Parasitic Infections ◽  
Developmental Cycle ◽  
Starting Point

Chagas disease is one of the most common parasitic infections in Latin America, which is transmitted by hematophagous triatomine bugs, of which Rhodnius prolixus is the vector prototype for the study of this disease. The protozoan parasite Trypanosoma cruzi, the etiologic agent of this disease, is transmitted by the vector to humans through the bite wound or mucosa. The passage of the parasite through the digestive tract of its vector constitutes a key step in its developmental cycle. Herewith, by a using high-throughput proteomic tool in order to characterize the midgut proteome of R. prolixus, we describe a set of functional groups of proteins, as well as the biological processes in which they are involved. This is the first proteomic analysis showing an elaborated hematophagy machinery involved in the digestion of blood, among which, several families of proteases have been characterized. The evaluation of the activity of cathepsin D proteases in the anterior part of the digestive tract of the insect suggested the existence of a proteolytic activity within this compartment, suggesting that digestion occurs early in this compartment. Moreover, several heat shock proteins, blood clotting inhibitors, and a powerful antioxidant enzyme machinery against reactive oxygen species (ROS) and cell detoxification have been identified. Highlighting the complexity and importance of the digestive physiology of insects could be a starting point for the selection of new targets for innovative control strategies of Chagas disease.

scholarly journals Structure Determination of Juvenile Hormone from Chagas Disease Vectors, Rhodnius prolixus and Triatoma infestans

2020 ◽  
Vol 49 (5) ◽  
pp. 538-541
Author(s):  
Keiji Matsumoto ◽  
Yoko Yasuno ◽  
Kohei Yasuda ◽  
Tsuyoshi Hayashi ◽  
Shin G. Goto ◽  
...  
Keyword(s):  
Juvenile Hormone ◽  
Chagas Disease ◽  
Structure Determination ◽  
Rhodnius Prolixus ◽  
Triatoma Infestans ◽  
Disease Vectors ◽  
Chagas Disease Vectors
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