scholarly journals Arbitrarily primed PCR fingerprinting of RNA and DNA in Entamoeba histolytica

2000 ◽  
Vol 42 (5) ◽  
pp. 249-253 ◽  
Author(s):  
Paulo Renato VALLE ◽  
Maria Betânia G. SOUZA ◽  
Edna M. PIRES ◽  
Edward F. SILVA ◽  
Maria A. GOMES
Keyword(s):  
Gene Expression ◽  
Rapd Analysis ◽  
Polymorphic Markers ◽  
Biological Features ◽  
Pcr Products ◽  
The Individual ◽  
Arbitrarily Primed Pcr ◽  
High Degree ◽  
Rna And Dna

Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the construction of dendograms. The dendogram obtained based on the RAPD products permitted the distinction of symptomatic and asymptomatic isolates, as well the correlation between the polymorphism exhibited and the virulence of the strains. The dendogram obtained for the RAP-PCR products did not show a correlation with the virulence of the strains but revealed a high degree of intraspecific transcriptional variability that could be related to other biological features, whether or not these are involved in the pathogenesis of amebiasis.

scholarly journals Genetic characterization of the domestic pig (Sus scrofa domestica) in Cerete-Colombia, using microsatellite markers

2014 ◽  
pp. 4150-4157
Author(s):  
Iván Meléndez G ◽  
Enrique Pardo P ◽  
Teodora Cavadia M
Keyword(s):  
Sus Scrofa ◽  
Polyacrylamide Gel Electrophoresis ◽  
Proteinase K ◽  
Domestic Pig ◽  
Expected Heterozygosity ◽  
Hair Samples ◽  
Pcr Products ◽  
High Degree ◽  
Sus Scrofa Domestica

ABSTRACTObjective. The purpose of this study was to characterize a population of domestic pig (Sus scrofa domestica) in Cereté, Córdoba, using 20 microsatellite; calculate heterozygosity per locus and average heterozygosity. Materials and methods. Hair samples were collected from 62 specimens. DNA was extracted by proteinase K digestion and phenol-chloroform purification. Information from 20 microsatellites was selected out of those recommended for swine biodiversity studies. PCR products were separated by a vertical polyacrylamide gel electrophoresis. The bands were visualized by staining with silver nitrate. Results. All microsatellites used were polymorphic. Between 3 (SW1067) and 15 (IFNG) alleles were detected with an average number of 6.7 and a total de 134 alleles. The average expected and observed heterozygosities were 0.5278 and 0.5479, respectively. PIC values ranged between 0.1999 and 0.8300 for loci SW1067 and SW911, respectively. Conclusions. Levels of observed and expected heterozygosity found in the present study indicate that the domestic pig (Sus scrofa domestica) in Córdoba Cereté show high degree of genetic variability

scholarly journals Characterization of Alstroemeria Species using Random Amplified Polymorphic DNA (RAPD) Analysis

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 482F-482 ◽  
Author(s):  
Deric D. Picton ◽  
Harrison G. Hughes
Keyword(s):  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Randomly Amplified Polymorphic Dna ◽  
Banding Patterns ◽  
Extraction Buffer ◽  
Rapd Pcr ◽  
High Degree ◽  
Species Hybrids ◽  
Color Variants

In this study, 11 species, hybrids, and color variants were characterized using randomly amplified polymorphic DNA (RAPD) analysis. Total genomic DNA was extracted using a 2% CTAB extraction buffer using fresh or frozen leaf material. The DNA was amplified using standard RAPD-PCR protocols utilizing 10-mer primers. All primers utilized exhibited a high degree of polymorphism in their banding patterns among the species and hybrids studied. The primers used produced ≈40 reproducible bands. It was possible to identify and uniquely distinguish all species and hybrids investigated using these bands.

scholarly journals Characterization of the Spatiotemporal Attributes of Sclerotinia Flower Blight Epidemics in a Perennial Pyrethrum Pathosystem

Plant Disease ◽  
2010 ◽  
Vol 94 (11) ◽  
pp. 1305-1313 ◽  
Author(s):  
Sarah J. Pethybridge ◽  
Frank S. Hay ◽  
David H. Gent
Keyword(s):  
Binomial Distribution ◽  
Management Strategies ◽  
Spatial Association ◽  
Sampling Unit ◽  
Data Sets ◽  
Estimated Parameters ◽  
Unit Scale ◽  
The Individual ◽  
High Degree

Sclerotinia flower blight, caused by Sclerotinia sclerotiorum, causes substantial losses in Australian pyrethrum fields. The spatiotemporal characteristics of epidemics were characterized in five fields over 3 years. Log likelihood tests indicated that the β-binomial distribution fit better than the binomial distribution for 92% of the data sets. The index of dispersion, D, was significantly greater than 1 in 97% of the data sets. The estimated parameters of the slope and intercept terms of the binary power law were 1.631 (standard error [SE] = 0.059) and 0.678 (SE = 0.099), indicating a high degree of aggregation at the individual sampling unit scale. In 69% of the data sets, the magnitude of the first-order autocorrelation coefficient [Formula: see text], was significantly greater than 0. In 11 of the 12 epidemics, the monomolecular model provided the best fit, indicative of monocyclic processes. A significant spatial association between apothecia and incidence of Sclerotinia flower blight within the lag of one sampling unit was also quantified. This study suggests that S. sclerotiorum apothecia emergence was closely synchronized with flower development, and epidemics were dominated by localized sources of ascosporic inoculums. This research provides the basis for improved management strategies for Sclerotinia flower blight in pyrethrum.

scholarly journals Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systems

2020 ◽  
Author(s):  
Timothy C. Yu ◽  
Winnie L. Liu ◽  
Marcia Brinck ◽  
Jessica E. Davis ◽  
Jeremy Shek ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inducible Promoter ◽  
Transcriptional Responses ◽  
Promoter Elements ◽  
Sequence Composition ◽  
Inducible Promoters ◽  
Mechanics Model ◽  
The Individual ◽  
Repressor Binding Site

AbstractA crucial step towards engineering biological systems is the ability to precisely tune the genetic response to environmental stimuli. In the case of Escherichia coli inducible promoters, our incomplete understanding of the relationship between sequence composition and gene expression hinders our ability to predictably control transcriptional responses. Here, we profile the expression dynamics of 8,269 rationally designed IPTG-inducible promoters that collectively explore the individual and combinatorial effects of RNA polymerase and LacI repressor binding site strengths. Using these data, we fit a statistical mechanics model that accurately models gene expression and reveals properties of theoretically optimal inducible promoters. Furthermore, we characterize three novel promoter architectures and show that repositioning binding sites within promoters influences the types of combinatorial effects observed between promoter elements. In total, this approach enables us to deconstruct relationships between inducible promoter elements and discover practical insights for engineering inducible promoters with desirable characteristics.

scholarly journals Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systems

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Timothy C. Yu ◽  
Winnie L. Liu ◽  
Marcia S. Brinck ◽  
Jessica E. Davis ◽  
Jeremy Shek ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inducible Promoter ◽  
Transcriptional Responses ◽  
Promoter Elements ◽  
Sequence Composition ◽  
Inducible Promoters ◽  
Mechanics Model ◽  
The Individual ◽  
Repressor Binding Site

AbstractA crucial step towards engineering biological systems is the ability to precisely tune the genetic response to environmental stimuli. In the case of Escherichia coli inducible promoters, our incomplete understanding of the relationship between sequence composition and gene expression hinders our ability to predictably control transcriptional responses. Here, we profile the expression dynamics of 8269 rationally designed, IPTG-inducible promoters that collectively explore the individual and combinatorial effects of RNA polymerase and LacI repressor binding site strengths. We then fit a statistical mechanics model to measured expression that accurately models gene expression and reveals properties of theoretically optimal inducible promoters. Furthermore, we characterize three alternative promoter architectures and show that repositioning binding sites within promoters influences the types of combinatorial effects observed between promoter elements. In total, this approach enables us to deconstruct relationships between inducible promoter elements and discover practical insights for engineering inducible promoters with desirable characteristics.

scholarly journals Structural Variability of Subtelomeres Applied for Genotyping of Selected Potato Varieties

2011 ◽  
Vol 41 (No. 1) ◽  
pp. 17-22
Author(s):  
K. Neplechová ◽  
E. Sýkorová ◽  
M. Novotná ◽  
J. Ptáček ◽  
J. Fajkus
Keyword(s):  
Specific Primers ◽  
Potato Varieties ◽  
Region I ◽  
Pcr Products ◽  
Subtelomeric Sequence ◽  
Internal Arrangement ◽  
The Individual ◽  
High Degree ◽  
Pcr Genotyping ◽  
Subtelomeric Regions

Subtelomeric regions reveal a relatively high degree of polymorphism due to the increased frequency of recombination events in these chromosome loci. In a search for molecular markers applicable to genotyping of potato varieties, we focused on two possible sources of polymorphism occurring in this region: (i) arrangement of blocks of subtelomeric chromatin; (ii) structure of telomere-subtelomere boundary. Analysis of the internal arrangement of subtelomeric sequences showed several types of cultivar-specific spectra of PCR products arising from the variant orientation of sequence units of the ST3-subtelomeric sequence, or from different lengths of regions linking the individual sequence units. Further, the telomere-subtelomere boundary sequences were amplified using telomeric and ST3-specific primers and the obtained products were cloned. Sequence analysis of the clones resulted in characterisation of a novel telomere-associated sequence (FIN2). Primers derived from this sequence were then used alone or in combination with telomeric or ST3-specific primers to generate cultivar-specific spectra of PCR products. The described combinations of sequence-specific primers may be used for the fast, cheap and reproducible PCR-genotyping of selected potato varieties.    

scholarly journals Molecular Characterization of Common Olive Varieties in Israel and the West Bank Using Randomly Amplified Polymorphic DNA (RAPD) Markers

1998 ◽  
Vol 123 (5) ◽  
pp. 837-841 ◽  
Author(s):  
Z. Wiesman ◽  
N. Avidan ◽  
S. Lavee ◽  
B. Quebedeaux
Keyword(s):  
Rapd Markers ◽  
Rapd Analysis ◽  
West Bank ◽  
The West ◽  
Mountain Ridge ◽  
Olive Trees ◽  
Olive Varieties ◽  
High Degree ◽  
Degree Of Similarity

RAPD analysis was performed on the main traditional and introduced varieties of olive grown in Israel and the West Bank. Primers were identified and used in combination to discriminate between different varieties. Significant biodiversity was demonstrated among `Nabali' olive trees growing along the central mountain ridge of the West Bank, suggesting that the grouping known as `Nabali' is actually a mixture of genetically distinct variants. On the other hand, RAPD profiles of selected variants of `Souri', cultivated mainly in the northern mountains of Israel, revealed a high degree of similarity, indicating that these variants represent environmental phenotypes of the same genome. Molecular differences were demonstrated between the `Nabali' group variants and `Souri'. Other more recently developed or introduced varieties showed individually distinct RAPD profiles. Possible future developments based on these data are mentioned.

scholarly journals Phylogenetic characterization of Babesia canis vogeli in dogs in the state of Goiás, Brazil

2011 ◽  
Vol 20 (4) ◽  
pp. 274-280 ◽  
Author(s):  
Sabrina Castilho Duarte ◽  
Juliana Alves Parente ◽  
Maristela Pereira ◽  
Célia Maria de Almeida Soares ◽  
Guido Fontgalland Coelho Linhares
Keyword(s):  
Molecular Similarity ◽  
Clinical Signs ◽  
Babesia Canis ◽  
Babesia Gibsoni ◽  
Phylogenetic Characterization ◽  
Pcr Products ◽  
High Degree ◽  
Veterinary Hospital ◽  
Babesia Canis Canis

The genus Babesia comprises protozoa that cause diseases known as babesiosis. Dogs are commonly affected by Babesia canis or Babesia gibsoni. Babesia canis is divided into the subspecies Babesia canis canis, Babesia canis vogeli and Babesia canis rossi. Among these, Babesia canis vogeli predominates in Brazil. The objective of this study was to conduct a phylogenetic analysis on Babesia isolates from dogs in Goiânia, Goiás. Blood samples were obtained from 890 dogs presenting clinical signs suggestive of canine babesiosis that were attended at a veterinary hospital of Goiás. Only samples presenting typical intraerythrocytic parasites were used in the study. These were subjected to DNA extraction and amplification of a fragment of the 18S rRNA, by means of PCR. The PCR products were purified and sequenced. Sequences were obtained from 35 samples but only 17 of these were kept after quality assessment. Similarity analysis using BLASTn demonstrated that all 17 sequences corresponded to B. canis vogeli. Analysis using the Mega4 software showed that the isolates of B. canis vogeli from dogs in Goiânia present a high degree of molecular similarity (99.2 to 100%) in comparison with other reference isolates from other regions of Brazil and worldwide, deposited in GenBank.

Characterization of RF Sputtered ZnO Piezoelectric Films Using Transmission Electron Microscope

1975 ◽  
Vol 33 ◽  
pp. 86-87
Author(s):  
Kemining W. Yeh ◽  
Richard S. Muller ◽  
Wei-Kuo Wu ◽  
Jack Washburn
Keyword(s):  
Integrated Circuit ◽  
Acoustic Waves ◽  
New Technology ◽  
Surface Acoustic Waves ◽  
Electromechanical Properties ◽  
Leading Role ◽  
Saw Devices ◽  
Transmission Electron ◽  
High Degree

Considerable and continuing interest has been shown in the thin film transducer fabrication for surface acoustic waves (SAW) in the past few years. Due to the high degree of miniaturization, compatibility with silicon integrated circuit technology, simplicity and ease of design, this new technology has played an important role in the design of new devices for communications and signal processing. Among the commonly used piezoelectric thin films, ZnO generally yields superior electromechanical properties and is expected to play a leading role in the development of SAW devices.

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