scholarly journals The "in vitro" antifungal activity evaluation of propolis G12 ethanol extract on Cryptococcus neoformans

2007 ◽  
Vol 49 (2) ◽  
pp. 93-95 ◽  
Author(s):  
Fabrício Freitas Fernandes ◽  
Amanda Latercia Tranches Dias ◽  
Cíntia Lacerda Ramos ◽  
Masaharu Ikegaki ◽  
Antonio Martins de Siqueira ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Cryptococcus Neoformans ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Biological Properties ◽  
Standard Strain ◽  
Antiviral Activities ◽  
Prolonged Antibiotic Therapy ◽  
In Vitro Antifungal Activity

Cryptococcosis is a worldwide disease caused by the etiological agent Cryptococcus neoformans. It affects mainly immunocompromised humans. It is relatively rare in animals only affecting those that have received prolonged antibiotic therapy. The propolis is a resin that can present several biological properties, including antibacterial, antifungal and antiviral activities. The standard strain C. neoformans ATTC 90112 was used to the antifungal evaluation. The tests were realized with propolis ethanol extract (PEE) G12 in concentrations from 0.1 to 1.6 mg mL-1. The evaluation of MIC and MFC were done according to DUARTE (2002)5. The inhibitory effect of PEE G12 on the fungal growing was seen at the concentration of 0.2 mg mL-1 and 1.6 mg mL-1 was considered a fungicidal one.

scholarly journals ANTIFUNGAL ACTIVITIES OF ETHANOL EXTRACT OF ROSEMARY LEAF (Rosemarinus officinalis L.) AGAINST Candida albicans

2020 ◽  
Vol 2 (1) ◽  
pp. 28
Author(s):  
Dewa Ayu Made Adnya Swari ◽  
I Wayan Martadi Santika ◽  
I Gusti Made Aman
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Rosmarinic Acid ◽  
Extraction Method ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Rosmarinus Officinalis ◽  
Control Group ◽  
Rosmarinus Officinalis L

Background: This study was a preliminary study aimed to investigating the in vitro activity of rosmarinic acid contained on Rosemary leaves (Rosemarinus officinalis L.) on the Candida albicans. Rosmarinic acid can be an inhibitor of the RTPase enzyme in the fungus Candida albicans. Objective: This study is expected to provide information in the development of science about the antifungal activity of ethanol extract of Rosemary leave (Rosmarinus officinalis L.) againsts C.albicans. The recent research was an laboratory experimental. Experiments on an ethanol extract of Rosemary leaves (Rosemarinus officinalis L.) and post-test design of the control group. Methods: The extraction method used in this study was a multilevel extraction method. The antifungal activity testing method uses discs and MIC methods. Replication was done six times at two concentration namely, 80% (b/v) and 100% for each measurement. Results: The data analysis was carried out using SPSS with 95% of confidence level with the Kruskal Wallis analysis then completed with the Mann Whitney U test. The results of the MIC experiment showed all the various concentrations of ethanol extract performed Candida albicans growth on the surface of the media. Extract with 80% (b/v) concentration showed greater antifungal activity. Furthermore the MIC was shown by 80% (b/v) concentration of the ethanol extract of Rosemary leaves against Candida albicans. The test results of the antifungal activity of the Rosemary leaf ethanol extract showed the presence of inhibitory diameter in the ethanol extract of the Rosemary leaf concentration of 80% (b/v) with a mean ± SD of 10.6 ± 0.57735 and RSD of 5.774%. Conclusion: The ethanol extract of Rosemary leaves (Rosmarinus officinalis L.) has an inhibitory effect on the fungus Candida albicans at a concentration of 80% (b / v) Keywords: Antifungal activity, Candida albicans, Rosemary, RTPase, ethanol extract

scholarly journals Antifungal Activity of Plumericin and Isoplumericin

2011 ◽  
Vol 6 (11) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Dharmendra Singh ◽  
Umakant Sharma ◽  
Parveen Kumar ◽  
Yogesh K Gupta ◽  
M. P. Dobhal ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Minimum Inhibitory Concentration ◽  
Antifungal Activity ◽  
Cryptococcus Neoformans ◽  
Inhibitory Concentration ◽  
Chloroform Extract ◽  
Drug Candidate ◽  
Minimum Fungicidal Concentration ◽  
In Vitro Antifungal Activity

This study evaluated the in vitro antifungal activity of the chloroform extract of Plumeria bicolor and its phytoconstituents plumericin and isoplumericin against Candida species and Cryptococcus neoformans by measuring the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC). Plumericin's consistently high activity against Candida albicans, C. krusei, C. glabrata, C. tropicalis and Cryptococcus neoformans was more potent than isoplumericin and the standard antifungal drug nystatin suggesting its potential as a drug candidate for candidiasis and cryptococcosis.

In vitro Antifungal Activity of Dihydropyrimidinones/Thiones Against Candida albicans and Cryptococcus neoformans

2020 ◽  
Vol 15 (6) ◽  
pp. 648-655
Author(s):  
Gabriel O. de Azambuja ◽  
Laura Svetaz ◽  
Itamar L. Gonçalves ◽  
Patricia F. Corbelini ◽  
Gilsane L. von Poser ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Drug Design ◽  
Cryptococcus Neoformans ◽  
Biginelli Reaction ◽  
Fungal Growth ◽  
Chemical Library ◽  
Antifungal Effect ◽  
In Vitro Antifungal Activity

Background: Since the Monastrol discovery in 1999 as the first inhibitor of Eg5, functionalized dihydropyrimidinones/thiones (DHPMs) have emerged as prototypes for drug design in different targets. The present work aimed to evaluate the antifungal activity of a chemical library of DHPMs. Methods: The compounds were obtained employing Biginelli reaction. Their antifungal activities were assessed against C. neoformans and C. albicans. Results: The compounds 1-i and 1-k inhibited moderately the fungal growth of C. neoformans, with compound 2-k presenting MIC80 values of 62.5-125 µg·mL-1. Considering activity against C. albicans, the compounds 1-i and 1-n present an MIC50 value of 125-250 µg·mL-1. Conclusion: The changes performed in DHPM scaffold appear to be valuable for generating compounds with potential antifungal effect.

In vitro antifungal activity of eugenol and vanillin against Candida albicans and Cryptococcus neoformans

1982 ◽  
Vol 28 (11) ◽  
pp. 1235-1241 ◽  
Author(s):  
Chuenchit Boonchird ◽  
T. W. Flegel
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Cryptococcus Neoformans ◽  
Tube Formation ◽  
Toxicity Tests ◽  
Short Term ◽  
Minimal Inhibitory Concentrations ◽  
The Mean ◽  
In Vitro Antifungal Activity

Eugenol and vanillin were examined for in vitro antifungal activity against the medically important yeasts, Candida albicans and Cryptococcus neoformans. Minimal inhibitory concentrations (MIC) and minimal fungicidal concentrations (MFC) were determined for each compound against 31 strains of C. albicans and 33 strains of C. neoformans. With eugenol, the mean MIC's for C. albicans and C. neoformans were 625 and 293 μg/mL, respectively, while the mean MFC's were 1209 and 521 μg/mL, respectively. With vanillin, the mean MIC's for C. albicans and C. neoformans were 1250 and 738 μg/mL, respectively, while the mean MFC's were 5000 and 1761 μg/mL, respectively. With C. albicans, inhibition and retardation of growth were similar for yeast and mycelial forms, but germ tube formation was inhibited at concentrations lower than those which inhibited growth. Short-term toxicity tests with mice using the intraperitoneal route gave maximum tolerated doses of 62.5 mg/kg for eugenol and 125 mg/kg for vanillin and excluded their use as therapeutic agents for systemic mycoses.

Prevention of fungal diseases in transgenic, bialaphos- and glufosinate-resistant creeping bentgrass (Agrostis palustris)

Weed Science ◽  
1998 ◽  
Vol 46 (1) ◽  
pp. 139-146 ◽  
Author(s):  
Chien-An Liu ◽  
Heng Zhong ◽  
Joseph Vargas ◽  
Donald Penner ◽  
Mariam Sticklen
Keyword(s):  
Antifungal Activity ◽  
Fungal Pathogens ◽  
Creeping Bentgrass ◽  
Inhibitory Effect ◽  
Sclerotinia Homoeocarpa ◽  
Agrostis Palustris ◽  
Symptomatic Infection ◽  
Simultaneous Control ◽  
In Vitro Antifungal Activity

The antifungal activity of the herbicides bialaphos and glufosinate, the active moiety of bialaphos, was assessed. Bialaphos showed a higher level of in vitro antifungal activity againstRhizoctonia solani, Sclerotinia homoeocarpa, andPythium aphanidermatumthan glufosinate. Glufosinate suppressed the mycelial growth ofR. solaniandS. homoeocarpa, but it had no inhibitory effect onP. aphanidermatumup to the highest concentration in our testing regimes. Various concentrations of bialaphos solutions were applied to transgenic, bialaphos- and glufosinate-resistant creeping bentgrass inoculated with fungal pathogens. Bialaphos applications were able to significantly reduce symptomatic infection byR. solaniandS. homoeocarpaon transgenic plants. Bialaphos significantly inhibitedP. aphanidermatum, but not to the same degree thatR. solaniandS. homoeocarpawere inhibited. These results indicate that bialaphos may provide a means for the simultaneous control of weeds and fungal pathogens in turf areas with transgenic, bialaphos-resistant creeping bentgrass.

scholarly journals Antifungal activity of Morinda citrifolia leaf extracts against Fusarium oxysporum

2020 ◽  
Vol 4 (1) ◽  
pp. 20-26
Author(s):  
Oktira Roka Aji ◽  
Yuni Rohmawati
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Morinda Citrifolia ◽  
Leaf Extracts ◽  
Mycelium Growth ◽  
Pathogen Control ◽  
Negative Impacts

Fusarium oxysporum is an important disease that causes wilt disease in plants. Fungal pathogen control using synthetic fungicides can cause negative impacts on the environment. Morinda citrifolia is one of the herbs that is known for many benefits. M. citrifolia leaf contains anthraquinone which have potential as antifungal agents. In this study, in vitro anti-fungal assay was conducted against F. oxysporum to analyze the antifungal activity of ethanol extract of M. citrifolia leaves. In vitro evaluation was carried out using poisoned food technique at four different concentrations i.e., 20%, 40%, 60% and 80%. The results showed concentration 60% gave the highest inhibitory effect (21,82%) on F. oxysporum mycelium growth.

scholarly journals Rutaceae: conservation at Eka Karya Bali Botanic Garden and its in vitro antifungal activity screening

2021 ◽  
Vol 6 (2) ◽  
pp. 108
Author(s):  
I Putu Agus Hendra Wibawa ◽  
Arrohmatus Syafaqoh Li'aini ◽  
Putri Sri Andila ◽  
Frelyta Ainuz Zahro'
Keyword(s):  
Antifungal Activity ◽  
Methanolic Extract ◽  
Pathogenic Fungi ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Botanic Garden ◽  
Murraya Paniculata ◽  
Activity Screening ◽  
In Vitro Antifungal Activity

Several species of Rutaceae have been widely used and commercialized in all regions in Indonesia. Some species of Rutaceae are consumed as fresh fruit and traditional medicine for various kinds of diseases, as well as to add aroma to various Indonesian culinary. Since 1959, Eka Karya Bali Botanic Garden (Eka Karya BBG) has successfully collected dozens of Rutaceae species with unknown potential. In addition to reporting the conservation of Rutaceae in Eka Karya BBG, this study aimed to screen the antifungal activity of Rutaceae methanolic extract toward Aspergillus niger, Cladosporium sp., and Fusarium solani. Leaves of 13 species of Rutaceae (Boenninghausenia sp., Citrus aurantifolia, C. maxima, C. medica, Clausena sp., Melicope sp., Micromelum sp., Murraya paniculata, Toddalia sp., Zanthoxylum sp., Z. alatum, Z. limonella, and Z. ovalifolium) were collected, cleaned, air-dried, soaked in methanol for three days, then evaporated using a rotary evaporator to obtain the plant crude extract. The in vitro inhibitory assay was conducted by the diffusion method. As a result, only C. medica, Clausena sp., and Z. limonella exhibited antifungal activity against those tested fungi. Their antifungal activity increased on day 2 post-treatment but slowly decreased on day 3. Thus, the result of this experiment can be used as preliminary data to researchRutaceae plant extracts as an alternative method to control pathogenic fungi. However, further research is needed to maintain and increase its inhibitory effect.

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