scholarly journals Prospective study of strongyloidosis in patients with hematologic malignancies

1997 ◽  
Vol 30 (5) ◽  
pp. 355-357 ◽  
Author(s):  
Carlos Graeff-Teixeira ◽  
Cristina S.M. Leite ◽  
Cristina L. Sperhacke ◽  
Katia Fassina ◽  
Simone M.G. Petry ◽  
...  
Keyword(s):  
Prospective Study ◽  
Hematological Malignancies ◽  
Severe Disease ◽  
Strongyloides Stercoralis ◽  
Hematologic Malignancies ◽  
Porto Alegre ◽  
Stool Samples ◽  
A Prospective Study ◽  
Disseminated Disease ◽  
Baermann Method

Immunocompromised individuals infected with Strongyloides stercoralis may develop severe hyperinfection or disseminated disease with high mortality. Patients with hematological malignancies are at risk because of immunodepression produced either by the disease or its treatment. A prospective study was undertaken at the Hospital de Clínicas de Porto Alegre, from July 1994 to July 1995. Seventy-two (HIV negative), had 3 stool samples collected at different days and had not received recent anti-helmintic therapy. Larvae, isolated in a modified Baermann method, were found in 6 patients, with a resultant prevalence of 8.3%. No complicated strongyloidosis was documented. The positive result for S. stercoralis larvae was significantly associated (p < 0.001) with eosinophilia. Knowledge of prevalence figures and incidence of severe disease is important to adequate guidelines for empirical treatment besides the rigorous search for strongyloidosis in patients with hematological malignancies.

A prospective study of characteristics and outcomes of bacteremia in patients with solid organ or hematologic malignancies

2013 ◽  
Vol 21 (9) ◽  
pp. 2521-2526 ◽  
Author(s):  
George Samonis ◽  
Konstantinos Z. Vardakas ◽  
Sofia Maraki ◽  
Giannoula S. Tansarli ◽  
Dimitra Dimopoulou ◽  
...  
Keyword(s):  
Prospective Study ◽  
Hematologic Malignancies ◽  
Solid Organ ◽  
A Prospective Study

Predictors of bone loss in childhood hematologic malignancies: a prospective study

2011 ◽  
Vol 23 (2) ◽  
pp. 665-674 ◽  
Author(s):  
Ghada El-Hajj Fuleihan ◽  
S. Muwakkit ◽  
A. Arabi ◽  
L. E.-O. Daouk ◽  
T. Ghalayini ◽  
...  
Keyword(s):  
Bone Loss ◽  
Prospective Study ◽  
Hematologic Malignancies ◽  
A Prospective Study

scholarly journals Evaluation of Two DNA Extraction Methods for Detection of Strongyloides stercoralis Infection

2018 ◽  
Vol 56 (4) ◽  
Author(s):  
Beatrice Barda ◽  
Rahel Wampfler ◽  
Somphou Sayasone ◽  
Khampheng Phongluxa ◽  
Syda Xayavong ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Strongyloides Stercoralis ◽  
Extraction Methods ◽  
Molecular Techniques ◽  
Diagnostic Methods ◽  
Content Type ◽  
Stool Samples ◽  
Dna Extraction Methods ◽  
Baermann Method

ABSTRACT Strongyloides stercoralis is present worldwide, but its prevalence is still uncertain, mainly due to the lack of sensitivity of diagnostic methods. Molecular techniques are under development, but a standardized protocol is still unavailable. We compared the sensitivity of real-time PCR, using two extraction protocols, with that of the Baermann technique. Samples were collected in the framework of the baseline screening of a randomized clinical trial evaluating moxidectin against S. stercoralis in Lao People's Democratic Republic. Two stool samples from each participant were processed by the Baermann method, and one subsample was processed by PCR. DNA was extracted using the QIAamp DNA stool minikit based on the standard protocol for the QIAamp DNA minikit (QIA) and using a modification of the QIA procedure (POL). Subsequently, all extracted samples were analyzed by real-time PCR. Overall, 95 samples were analyzed by the three diagnostic methods. Sixty-nine (72.6%) samples were positive according to the Baermann method, 25 (26.3%) by the QIA method, and 62 (65.3%) by the POL method. The sensitivities were 86% (95% confidence interval [CI], 76.7 to 92.9), 31.0% (95% CI, 21.3 to 42.6), and 78.0% (95% CI, 66.8 to 86.1) for the Baermann, QIA, and POL methods, respectively. The sensitivities calculated for each day of the Baermann method separately were 60% (48.4 to 70.8%) and 64% (52.2 to 74.2%) for days 1 and 2, respectively. In conclusion, the POL method revealed a good performance and was comparable to the Baermann test performed on two stool samples and superior to the Baermann method performed on one stool sample. Additional studies are needed to standardize a PCR protocol for S. stercoralis diagnosis.

Prevalence of Human Intestinal Parasitic Nematode Among OutPatients Attending Wudil General Hospital, Kano State, Nigeria

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
Lurwan Muazu ◽  
Yahaya Abdullahi ◽  
Zaharaddeen Umar
Keyword(s):  
General Hospital ◽  
Prevalence Rate ◽  
Parasitic Nematode ◽  
Age Groups ◽  
Strongyloides Stercoralis ◽  
Parasitic Nematodes ◽  
National Immunization ◽  
Stool Samples ◽  
The Difference ◽  
A Prospective Study

A prospective study was carried out to determine the prevalence of human intestinal parasitic nematodes among outpatients attending Wudil General Hospital, Wudil Local Government Area of Kano State, Nigeria. A total of 56 stool samples were randomly collected from the outpatients; processed and examined (macroscopic and microscopic) by formal ether sedimentation techniques. The prevalence of human intestinal parasitic nematode among the patient in the study area was 46.4%. The Males had the highest (48.98%) infection rate, while females had the least (28.6%) prevalence rate, however, this is not statistically significant (p>0.05). The 36-40years age groups had the highest prevalence of 75%, while 21-25years age groups had the least prevalence rate of 25%, the difference in prevalence among the ages was found to be statistically not significant (p>0.05). Strongyloides stercoralis had the highest prevalence of 30.36% while Trichuris trichiura had the least prevalence rate of 3.57%, the differences among the species of human intestinal parasitic nematode was found to be statistically not significant (p>0.05). The control of human intestinal parasitic nematode should be done by adopting drug treatment for those already infected similar to the national immunization program, improve standard sanitation and health services in Wudil L.G.A, particularly the rural area.

Pain and anxiety associated with bone marrow aspiration and biopsy: a prospective study on 152 Italian patients with hematological malignancies

2011 ◽  
Vol 90 (10) ◽  
pp. 1233-1235 ◽  
Author(s):  
Gregorio Antonio Brunetti ◽  
Andrea Tendas ◽  
Elisabetta Meloni ◽  
Daniela Mancini ◽  
Paolo Maggiore ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Prospective Study ◽  
Hematological Malignancies ◽  
Bone Marrow Aspiration ◽  
A Prospective Study

Prospective study applying spoligotyping directly to DNA from sputum samples of patients suspected of having tuberculosis

2009 ◽  
Vol 55 (7) ◽  
pp. 895-900 ◽  
Author(s):  
Patricia Izquierdo Cafrune ◽  
Lia Gonçalves Possuelo ◽  
Andrezza Wolowski Ribeiro ◽  
Marta Osório Ribeiro ◽  
Gisela Unis ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Prospective Study ◽  
Clinical Setting ◽  
Turnaround Time ◽  
Clinical Usefulness ◽  
Porto Alegre ◽  
Lower Accuracy ◽  
A Prospective Study

A prospective study was designed to evaluate the clinical usefulness of spoligotyping applied directly to sputum samples. Patients suspected of having tuberculosis were recruited at the Hospital Sanatorio Partenon in Porto Alegre, Brazil. Of the 197 samples included in the analysis, 175 (88.8%) yielded a spoligotyping result that fully matched that obtained from culture. Low bacillary samples presented lower accuracy (50%). From 135 Mycobacterium tuberculosis spoligopatterns, we identified 44 different spoligotypes, of which 21 were shared patterns and 23 were unique. T1 was the most frequent subfamily. The genotyping strategy proposed here presents a short turnaround time and could be helpful in providing rapid information on strain identities in a clinical setting.

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