scholarly journals Evaluation of the phagocytic activity of peripheral blood monocytes of patients with Jorge Lobo's disease

2004 ◽  
Vol 37 (2) ◽  
pp. 165-168 ◽  
Author(s):  
Fátima Regina Vilani-Moreno ◽  
Luciana Moreira Silva ◽  
Diltor Vladimir Araújo Opromolla
Keyword(s):  
Incubation Time ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Healthy Subjects ◽  
Mononuclear Cells ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Significant Difference ◽  
Host Parasite ◽  
And Control

Studies on host-parasite interaction in Jorge Lobo's disease are scarce, with no report in the literature on the phagocytosis of Lacazia loboi by phagocytic mononuclear cells. Thus, the objective of the present study was to assess the phagocytic activity of blood monocytes in the presence of L. loboi in patients with the disease and in healthy subjects (controls) over 3 and 24 hours of incubation. Statistical analyses of the results showed no significant difference in percent phagocytosis of the fungus between patient and control monocytes. With respect to incubation time, however, there was a significant difference, in that percent phagocytosis was higher at 3 hours than at 24 hours (p <0.01). These results suggest that monocytes from patients with the mycosis are able to phagocyte the fungus, as also observed in control individuals.

scholarly journals Glucocerebrosidase Activity is Reduced in Cryopreserved Parkinson’s Disease Patient Monocytes and Inversely Correlates with Motor Severity

2021 ◽  
pp. 1-9
Author(s):  
Laura P. Hughes ◽  
Marilia M.M. Pereira ◽  
Deborah A. Hammond ◽  
John B. Kwok ◽  
Glenda M. Halliday ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Rating Scale ◽  
Disease Patient ◽  
Motor Symptom ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Classical Monocytes

Background: Reduced activity of lysosomal glucocerebrosidase is found in brain tissue from Parkinson’s disease patients. Glucocerebrosidase is also highly expressed in peripheral blood monocytes where its activity is decreased in Parkinson’s disease patients, even in the absence of GBA mutation. Objective: To measure glucocerebrosidase activity in cryopreserved peripheral blood monocytes from 30 Parkinson’s disease patients and 30 matched controls and identify any clinical correlation with disease severity. Methods: Flow cytometry was used to measure lysosomal glucocerebrosidase activity in total, classical, intermediate, and non-classical monocytes. All participants underwent neurological examination and motor severity was assessed by the Movement Disorders Society Unified Parkinson’s Disease Rating Scale. Results: Glucocerebrosidase activity was significantly reduced in the total and classical monocyte populations from the Parkinson’s disease patients compared to controls. GCase activity in classical monocytes was inversely correlated to motor symptom severity. Conclusion: Significant differences in monocyte glucocerebrosidase activity can be detected in Parkinson’s disease patients using cryopreserved mononuclear cells and monocyte GCase activity correlated with motor features of disease. Being able to use cryopreserved cells will facilitate the larger multi-site trials needed to validate monocyte GCase activity as a Parkinson’s disease biomarker.

scholarly journals The effect of human amniotic epithelial cell on dendritic cell differentiation of peripheral blood monocytes: An experimental study

2020 ◽  
Author(s):  
Bahare Keshavarzi ◽  
Meraj Tabatabaei ◽  
Amir Hasan Zarnani ◽  
Fahime Ramezani Tehrani ◽  
Mahmood Bozorgmehr ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Amniotic Membrane ◽  
Peripheral Blood ◽  
Normal Pregnancy ◽  
Interleukin 4 ◽  
Control Group ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Dendritic Cell Differentiation ◽  
Significant Difference

Background: The amniotic membrane plays an important role in maintaining a healthy pregnancy. The main population cells from amniotic membrane include human amnion epithelial cells (hAECs) which have been shown to possess immunomodulatory properties. Objective: The proximity of hAECs with monocyte leads to the generation of tollerogenic dendritic cells. Materials and Methods: hAECs were obtained from normal pregnancy. Peripheral blood monocytes were isolated by anti-CD14 MACS method. Co-cultures of monocytes and hAECs were established in Transwell chambers supplemented with granulocytemacrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) in the absence and presence of lipopolysaccharide (LPS) to produce immature and mature DCs, respectively. Immunophenotyping of the obtained DCs was done through flow cytometry and the production of cytokines was measured by ELISA. Mixed leukocyte Reaction (MLR) was also performed for the functional assessment of DCs. Results: Immunophenotyping of [hAECs - Immature DC (iDC)] and [hAECs - iDC] + LPS cells revealed that the expression of CD1a, CD80, CD86, CD40, HLA-DR, and CD83 markers showed no significant difference as compared with the control group (iDCs and mDCs alone). In the [hAECs-iDCs] + LPS cells, the percentage of CD14 cells at the ratio of 1:2.5 showed significant differences compared to the control group. The production of IL-10 and IL-12 showed no significant difference in any of the cultures as compared to the control groups. Also, co-cultured DCs did not inhibit proliferation of lymphocyte. Conclusion: Our findings show that factors secreted from cultured hAECs are unable to generate of tollerogenic dendritic cells. To achieve a better understanding of other mechanisms more investigations are needed. Key words: Amniotic membrane, Dendritic cells, Human placenta, Immunomodulation, Monocyte.

scholarly journals Changes of Regulatory T and B Cells in Patients with Papillary Thyroid Carcinoma after131I Radioablation: A Preliminary Study

2013 ◽  
Vol 2013 ◽  
pp. 1-8
Author(s):  
Lei Jiang ◽  
Yanxia Zhan ◽  
Yusen Gu ◽  
Yi Ye ◽  
Yunfeng Cheng ◽  
...  
Keyword(s):  
B Cells ◽  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
B Cell ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Papillary Thyroid ◽  
T And B Cells ◽  
Significant Difference ◽  
And Control

Introduction. Lymphocytic infiltration and specific lymphocytes subsets may play important roles in papillary thyroid carcinoma (PTC) progression and prognosis. In this study, we try to understand the influence of131I radioablation on the important lymphocytes subtypes of regulatory T and B cells (Tregs and Bregs).Methods. Peripheral blood mononuclear cells from 30 PTC patients before and after131I therapy, and 20 healthy donors were collected. The expression of Tregs (CD4+CD25+CD127-/low) and B cell (CD5+CD19+) and production and secretion of interleukin 10 (IL-10) were analyzed by FACS and ELISA assay, respectively.Results. For Tregs percentage in peripheral blood lymphocytes, there was no difference between pretreatment and control and between posttreatment and control. Compared with pretherapy, increased Tregs infiltration was noted in posttherapy (P<0.05). Although no difference was between pretreatment and control, compared with these two groups, decreased CD19+and CD5+CD19+B cell percentage in posttreatment was observed (P<0.05). Among these groups, no significant difference was displayed in intracellular IL-10 production and extracellular IL-10 secretion.Conclusions.131I Radioablation increased Tregs and decreased CD19+and CD5+CD19+B cells percentage after treatment. However, it has no effect on IL-10 and lymphocytes in peripheral blood. Therefore, longer follow-up of Tregs and Bregs should be further investigated.

Increased phagocytic activity of peripheral blood monocytes after intravenous injection of phospholipase A2 to monkeys

1991 ◽  
Vol 28 (1) ◽  
pp. 5-9 ◽  
Author(s):  
Alejandro Bravo-Cuellar ◽  
Françoise Homo-Delarche ◽  
Rodolfo Ramos-Zepeda ◽  
Pierre Dubouch ◽  
Jeannine Cabannes ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Intravenous Injection ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes

Respiratory burst in alveolar macrophages of diabetic rats

1990 ◽  
Vol 68 (6) ◽  
pp. 2384-2390 ◽  
Author(s):  
V. Mohsenin ◽  
J. Latifpour
Keyword(s):  
Alveolar Macrophages ◽  
Respiratory Burst ◽  
Peripheral Blood ◽  
Diabetic Rats ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Significant Difference ◽  
Cytochrome C Reduction ◽  
Generating Capacity ◽  
O2 Production

Bactericidal ability of alveolar macrophages is depressed in rats with diabetes mellitus. To define the mechanism of this abnormality, we measured the parameters of respiratory burst in alveolar macrophages, peripheral blood monocytes, and neutrophils of rats 8 wk after the induction of diabetes by streptozocin. Superoxide anion (O2-.) generation during basal conditions and after stimulation with phorbol myristate acetate (PMA) was measured as superoxide dismutase-inhibitable cytochrome c reduction. NADPH, the principal substrate for NADPH-oxidase-dependent O2-. generation, was measured in the alveolar macrophages and quick-frozen lungs by the enzyme-cycling method. O2-. generation after PMA was significantly lower in the alveolar macrophages of diabetics than in the controls (14.4 +/- 2.0 nmol.10(6) cells-1.20 min-1 vs. 26.2 +/- 1.9, P less than 0.05). Conversely the peripheral blood monocytes of diabetics demonstrated an enhanced O2-. production after PMA stimulation. There was no significant difference in the neutrophil O2-.-generation between the groups. The alveolar macrophage NADPH (control 0.44 +/- 0.15 nmol/10(6) cells vs. diabetic 0.21 +/- 0.04, P less than 0.05) and lung tissue NADPH levels (control 81.4 +/- 16.3 nmol/g dry wt vs. diabetic 35.8 +/- 20.5, P less than 0.05) were significantly lower in the diabetics than in the controls. These data indicate that the O2-.-generating capacity of alveolar macrophages is markedly depressed in diabetes, whereas their precursors, monocytes, are primed to generate O2-. with PMA stimulation.(ABSTRACT TRUNCATED AT 250 WORDS)

Effects of Culture Supernatants of Leukocytes from Periodontal Patients on Monocyte-Mediated Hydroxyapatite Resorption

1988 ◽  
Vol 2 (2) ◽  
pp. 368-371
Author(s):  
Y. Marumoto ◽  
I. Sato ◽  
K. Ikeda
Keyword(s):  
Periodontal Disease ◽  
Peripheral Blood ◽  
Healthy Subjects ◽  
Mononuclear Cells ◽  
Healthy Controls ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Significant Difference ◽  
Culture Supernatants

In this study, the effects of culture supernatants on various activities of the monocyte, as a bone-resorbing cell, were compared between peripheral blood leukocyte (PBL) cultures from patients with periodontal disease and those from subjects with a clinically healthy periodontium. We have reported that normal human monocytes in vitro induce the release of calcium from synthetic hydroxyapatite particles and that the activity is enhanced by supernatants from cultures of stimulated or non-stimulated peripheral blood leukocytes. Monocytes from both patients and healthy subjects induced the release of calcium from hydroxyapatite particles (HA) to an equal degree. This activity of monocytes from healthy subjects showed a statistically significant increase by addition of supernatants from stimulated or unstimulated cultures of peripheral blood leukocytes from periodontitis patients. This increase was greater than that seen with supernatants from cells of healthy controls. The Nitro Blue Tetrazolium reduction activity and [3H]-thymidine incorporation of monocytes were also increased by addition of the supernatants from leukocyte cultures from either patients or healthy controls, but no significant difference was noted in the increase. These results suggest that the HA-resorbing activity of monocytes was enhanced by factors from cultured leukocytes. Furthermore, these studies showed that production of these factors by peripheral mononuclear cells from patients with periodontal disease was greater than that seen with cells from normal subjects.

INCREASED PRODUCTION OF PROCOAGULANT ACTIVITY BY PERIPHERAL BLOOD MONOCYTES IN HUMAN AND EXPERIMENTAL OBSTRUCTIVE JAUNDICE

1987 ◽  
Author(s):  
N Semeraro ◽  
P Montemurro ◽  
G Chetta ◽  
D Altomare ◽  
D Giordano ◽  
...  
Keyword(s):  
Obstructive Jaundice ◽  
Peripheral Blood ◽  
Prognostic Significance ◽  
Mononuclear Phagocytes ◽  
Procoagulant Activity ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Duct Ligation ◽  
Significant Difference ◽  
Low Levels

Fibrin formation and subsequent microvascular thrombosis are important pathogenetic factors in renal failure associated with severe obstructive jaundice (OJ) particularly after surgery, but the mechanism of blood clotting activation is poorly understood. We have studied the procoagulant activity (PCA) of peripheral blood monocytes (M) in 35 patients with severe OJ (serum bilirubin > 8 mg% ) and in 27 nonjaundiced control patients, using a one-stage clotting assay. Monocytes from jaundiced patients, tested immediately after isolation, expressed low levels of PCA (7.3 ± 2.0 u/ 105 M) which was, however, significantly higher than in cells from controls (2.5 ± 0.4 u; p<0.05). In addition, following incubation in short-term cultures with and without endotoxin, they generated significantly more PCA than did control cells (p< 0.005) No significant difference in PCA was found between patients with and without malignancy in either group. In rabbits made icteric by bile duct ligation (15 days), the endotoxin-induced monocyte PCA was markedly increased as compared to sham-operated animals ( p<0.05). In all instances PCA was identified as tissue factor. When related to the clinical outcome of the disease, PCA was about 3-fold higher in the jaundiced patients who died than in the survivors (p<0.01). All patients with a fatal evolution had more than 500 u of endotoxin-induced PCA/105 M; such high levels of PCA were found only in 26% of icteric, uncomplicated patients and in 4% of controls (all without complications). The increased capacity of mononuclear phagocytes to produce PCA might help explain activation of blood coagulation in severe 0J. The association between exceedingly high levels of PCA and lethal outcome suggests that PCA may have a prognostic significance.

Effect of antiinflammatory drugs on the phagocytic activity of peripheral blood monocytes

1982 ◽  
Vol 9 (3) ◽  
pp. 321-330 ◽  
Author(s):  
B. Klein ◽  
A. Tiomny ◽  
A. Globerson ◽  
I. Elian ◽  
I. Notti ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Blood Monocytes ◽  
Antiinflammatory Drugs ◽  
Peripheral Blood Monocytes

scholarly journals Phenotypic and genetic evaluation of human monocyte-derived dendritic cells generated from whole blood for immunotherapy

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Yousri M. Hussein ◽  
Doaa M. Hendawy ◽  
Abdalrahman N. Alghamdy ◽  
Nermin Raafat
Keyword(s):  
Flow Cytometry ◽  
Dendritic Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Surface Antigens ◽  
Interleukin 4 ◽  
Blood Monocytes ◽  
Hla Dr ◽  
Significant Difference

Abstract Background Dendritic cells (DCs) recognize different pathogens and cancer cells and activate the adaptive immune response. The generation of effective DC-based cancer vaccines depends on the appropriate differentiation of monocytes in vitro. This study aimed to standardize a protocol for the in vitro differentiation of human peripheral blood monocytes into immature DCs upon treatment with growth factors and generate monocyte-derived DCs (MoDCs). Peripheral blood mononuclear cells were separated from peripheral blood. After monocyte enrichment by plastic adhesion, monocytes were cultured for 6 days in the presence of granulocyte-macrophage colony-stimulating factor and interleukin-4 to generate immature DCs. The cells were examined by microscopy. Using flow cytometry, DCs were evaluated for the expression of the CD83 and HLA-DR surface antigens, for the uptake of fluorescein isothiocyanate conjugated dextran, and also for the expression of CD80 and CD86 mRNA. Results CD80 and CD86 genes expression was upregulated at day six and exhibited a significant difference (P < 0.05). DCs showed positive expression of the CD83 and HLA-DR surface antigens by flow cytometry and FITC-conjugated dextran uptake. Conclusion This study represents a preliminary trial to generate immature MoDCs in vitro from blood monocytes collected by the flask adherence method. It offers a panel of surface markers for DCs characterization and provides Immature DCs for experimental procedures after 6 incubation days.

scholarly journals The Oxidative Response of Human Monocytes to Surface Modified Commercially Pure Titanium

2021 ◽  
Vol 12 ◽  
Author(s):  
Robert P. De Poi ◽  
Michael Kowolik ◽  
Yoshiki Oshida ◽  
Karim El Kholy
Keyword(s):  
Incubation Time ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Pure Titanium ◽  
Commercially Pure Titanium ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Commercially Pure ◽  
Surface Modified ◽  
Secondary Stimulation

Cellular responses to implanted biomaterials are key to understanding osseointegration. The aim of this investigation was to determine the in vitro priming and activation of the respiratory burst activity of monocytes in response to surface-modified titanium. Human peripheral blood monocytes of healthy blood donors were separated, then incubated with surface-modified grade 2 commercially pure titanium (CPT) disks with a range of known surface energies and surface roughness for 30- or 60-min. Secondary stimulation by phorbol 12-myrisate 13-acetate (PMA) following the priming phase, and luminol-enhanced-chemiluminescence (LCL) was used to monitor oxygen-dependent activity. Comparison among groups was made by incubation time using one-way ANOVA. One sample from each group for each phase of the experiment was viewed under scanning electron microscopy (SEM) and qualitative comparisons made. The results indicate that titanium is capable of priming peripheral blood monocytes following 60-min incubation. In contrast, 30 min incubation time lead to reduced LCL on secondary stimulation as compared to cells alone. At both time intervals, the disk with the lowest surface energy produced significantly less LCL compared to other samples. SEM examination revealed differences in surface morphology at different time points but not between differently surface-modified disks. These results are consistent with the hypothesis that the titanium surface characteristics influenced the monocyte activity, which may be important in regulating the healing response to these materials.

Sign in / Sign up

Export Citation Format

Share Document