scholarly journals Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence

2013 ◽  
Vol 48 (3) ◽  
pp. 270-279 ◽  
Author(s):  
Carlos Alexandre Gomes Ribeiro ◽  
Janaína Paula Marques Tanure ◽  
Talles Eduardo Ferreira Maciel ◽  
Everaldo Gonçalves de Barros
Keyword(s):  
Polymorphic Information Content ◽  
Low Cost ◽  
High Accuracy ◽  
Genetic Purity ◽  
Phenotypic Data ◽  
Soybean Cultivars ◽  
Highly Correlated ◽  
Semiautomated Method

The objective of this work was to standardize a semiautomated method for genotyping soybean, based on universal tail sequence primers (UTSP), and to compare it with the conventional genotyping method that uses electrophoresis in polyacrylamide gels. Thirty soybean cultivars were genotypically characterized by both methods, using 13 microsatellite loci. For the UTSP method, the number of alleles (NA) was 50 (2-7 per marker) and the polymorphic information content (PIC) ranged from 0.40 to 0.74. For the conventional method, the NA was 38 (2-5 per marker) and the PIC varied from 0.39 to 0.67. The genetic dissimilarity matrices obtained by the two methods were highly correlated with each other (0.8026), and the formed groups were coherent with the phenotypic data used for varietal registration. The 13 markers allowed the distinction of all analyzed cultivars. The low cost of the UTSP method, associated with its high accuracy, makes it ideal for the characterization of soybean cultivars and for the determination of genetic purity.

scholarly journals Characterization of handmade Nepali paper as a platform for paper analytical device to determine anti-diabetic drug

2021 ◽  
Author(s):  
Ram Bhattarai ◽  
Sanam Pudasaini ◽  
Mukesh Sah ◽  
Bhanu Neupane ◽  
Basant Giri
Keyword(s):  
Limit Of Detection ◽  
Low Cost ◽  
Water Contact ◽  
Regulatory Standards ◽  
Three Samples ◽  
Limit Of Quantitation ◽  
Metformin Concentration ◽  
Analytical Device

The COVID-19 pandemic has highlighted the need of eco-friendly and locally or distributed manufacturing of diagnostic and safety products. Here, we characterized five handmade papers for their potential application to make paper analytical device (PADs). The handmade papers were made from locally available plant fiber using eco-friendly method. Thickness, grammage, and apparent density of the paper samples ranged from 198 μm to 314 μm, 49 g/m2 to 117.8 g/m2, and 0.23 to 0.39 g/cm3, respectively. Moisture content, water filtration and wicking speed ranged from 5.2% to 7.1%, 35.7 to 156.7, and 0.062 to 0.124 mms-1, respectively. Further, water contact angle and porosity ranged from 76˚ to 112˚ and 79% to 83%, respectively. The best paper sample one was chosen to fabricate PADs which were used for the determination of metformin. The metformin assay on PADs followed linear range from 0.0625 to 0.5 mg/mL. The assay had limit of detection and limit of quantitation of 0.05 mg/mL and 0.18 mg/mL respectively. The new method was used to test metformin samples (n=20) collected from local pharmacies. The average amount of metformin concentration in samples was 465.6 ± 15.1mg/tablet. Three samples did not meet the regulatory standards. When compared with spectrophotometric method, PADs assay correctly predicted 18 out of 20 samples. The PADs assay on handmade paper may provide a low-cost and easy-to-use system to screening the quality of drugs and other point-of-need applications.

Determination of Satranidazole through Ion-Associative Complex Reaction

2019 ◽  
Vol 4 (1) ◽  
pp. 23-27
Author(s):  
K. Kiran Kumar ◽  
R. Venkata Nadh ◽  
M. Siva Kishore ◽  
G. Giri Prasad
Keyword(s):  
Statistical Analysis ◽  
Low Cost ◽  
Ion Association ◽  
Pharmaceutical Formulations ◽  
High Accuracy ◽  
Complex Reaction ◽  
Colored Complex ◽  
Absorbance Maximum ◽  
Association Complex

A simple, selective, accurate and low-cost spectrophotometric method has been described for determination of satranidazole in bulk and pharmaceutical formulations. The developed method involves the formation of chloroform extractable colored ion-association complex of satranidazole with Tropaeolin OOO (TPooo). The extracted colored complex showed absorbance maximum at wavelength 484 nm and obeying Beer′s law in the concentration 4-20 μg mL-1 with the correlation coeffiecent of 0.9998. The results of statistical analysis of the proposed method reveals high accuracy and good precession. Thus, the proposed method can be used commercially for the determination of satranidazole in bulk and pharmaceutical formulations.

Amperometric nanobiosensor for quantitative determination of glyphosate and glufosinate residues in corn samples

2009 ◽  
Vol 81 (1) ◽  
pp. 123-139 ◽  
Author(s):  
Everlyne A. Songa ◽  
Vernon S. Somerset ◽  
Tesfaye Waryo ◽  
Priscilla G. L. Baker ◽  
Emmanuel I. Iwuoha
Keyword(s):  
Quantitative Determination ◽  
Low Cost ◽  
Sensor Response ◽  
Synthesis And Characterization ◽  
Disk Electrode ◽  
Injection Volume ◽  
Long Period ◽  
Amperometric Method

This study presents a simple, sensitive, rapid, and low-cost amperometric method for direct and quantitative determination of glyphosate and glufosinate herbicides. Electrochemical synthesis and characterization of poly(2,5-dimethoxyaniline)-poly(4-styrenesulfonic acid) (PDMA-PSS) nanoparticles was achieved by cyclic voltammetry (CV) and scanning electron microscopy (SEM). The nanobiosensor was constructed by immobilizing the enzyme horseradish peroxidase (HRP) electrostatically onto the surface of a rotating gold disk electrode modified with PDMA-PSS nanoparticles. The biosensing principle was based on determination of the sensor response to glyphosate and glufosinate by amperometric methods. Hydrogen peroxide (H2O2) was used to measure activity of the enzyme before injection of the herbicides into the electrolyte solution. The enzyme electrode was stable for a long period of time and was used for over 60 measurements. Glyphosate and glufosinate analyses were realized on spiked corn samples within a concentration range of 2.0-78.0 μg L-1, corroborating that the nanobiosensor is sensitive enough to detect herbicides in these matrices. Based on a 20-μL sample injection volume, the detection limits were 0.1 μg L-1 (10-10 M) for both glyphosate and glufosinate without sample clean-up or preconcentration.

Atom Probe Analysis of III–V and Si-Based Semiconductor Photovoltaic Structures

2007 ◽  
Vol 13 (6) ◽  
pp. 493-502 ◽  
Author(s):  
Brian P. Gorman ◽  
Andrew G. Norman ◽  
Yanfa Yan
Keyword(s):  
High Efficiency ◽  
Low Cost ◽  
Depth Profiling ◽  
Compositional Analysis ◽  
Atom Probe ◽  
Photovoltaic Devices ◽  
Chemical Profiling ◽  
Semiconductor Conductivity

The applicability of atom probe to the characterization of photovoltaic devices is presented with special emphasis on high efficiency III–V and low cost ITO/a-Si:H heterojunction cells. Laser pulsed atom probe is shown to enable subnanometer chemical and structural depth profiling of interfaces in III–V heterojunction cells. Hydrogen, oxygen, and phosphorus chemical profiling in 5-nm-thick a-Si heterojunction cells is also illustrated, along with compositional analysis of the ITO/a-Si interface. Detection limits of atom probe tomography useful to semiconductor devices are also discussed. Gaining information about interfacial abruptness, roughness, and dopant profiles will allow for the determination of semiconductor conductivity, junction depletion widths, and ultimately photocurrent collection efficiencies and fill factors.

Detection of Circulating Tissue Factor and Factor VII in a Normal Population

1996 ◽  
Vol 75 (05) ◽  
pp. 772-777 ◽  
Author(s):  
Sybille Albrecht ◽  
Matthias Kotzsch ◽  
Gabriele Siegert ◽  
Thomas Luther ◽  
Heinz Großmann ◽  
...  
Keyword(s):  
Tissue Factor ◽  
Normal Population ◽  
Mean Value ◽  
Factor Vii ◽  
Significant Difference ◽  
Age Dependent ◽  
The Mean ◽  
Highly Correlated ◽  
And Gender

SummaryThe plasma tissue factor (TF) concentration was correlated to factor VII concentration (FVIIag) and factor VII activity (FVIIc) in 498 healthy volunteers ranging in age from 17 to 64 years. Immunoassays using monoclonal antibodies (mAbs) were developed for the determination of TF and FVIIag in plasma. The mAbs and the test systems were characterized. The mean value of the TF concentration was 172 ± 135 pg/ml. TF showed no age- and gender-related differences. For the total population, FVIIc, determined by a clotting test, was 110 ± 15% and the factor VIlag was 0.77 ± 0.19 μg/ml. FVII activity was significantly increased with age, whereas the concentration demonstrated no correlation to age in this population. FVII concentration is highly correlated with the activity as measured by clotting assay using rabbit thromboplastin. The ratio between FVIIc and FVIIag was not age-dependent, but demonstrated a significant difference between men and women. Between TF and FVII we could not detect a correlation.

Characterization of Impressed Current Technique to Model Corrosion of Reinforcement in Concrete

2020 ◽  
Vol 11 (1) ◽  
pp. 93-99
Author(s):  
Abu Zakir Morshed ◽  
Sheikh Shakib ◽  
Tanzim Jahin
Keyword(s):  
Immersion Time ◽  
Chloride Content ◽  
Engineering Science ◽  
Coastal Regions ◽  
Faraday’S Law ◽  
Current Technique ◽  
Corrosion Cell ◽  
Impressed Current

Corrosion of reinforcement is an important durability concern for the structures exposed to coastal regions. Since corrosion of reinforcement involves long periods of time, impressed current technique is usually used to accelerate the corrosion of reinforcement in laboratories. Characterization of impressed current technique was the main focus of this research,which involved determination of optimum chloride content and minimum immersion time of specimens for which the application of Faraday’s law could be efficient. To obtain optimum chloride content, the electrolytes in the corrosion cell were prepared similar to that of concrete pore solutions. Concrete prisms of 200 mm by 200 mm by 300 mm were used to determine the minimum immersion time for saturation. It was found that the optimum chloride content was 35 gm/L and the minimum immersion time for saturation was 140 hours. Accounting the results, a modified expression based on Faraday’s law was proposed to calculate weight loss due to corrosion. Journal of Engineering Science 11(1), 2020, 93-99

The Determination of Sense via Deleuze and Blanchot: Paradoxes of the Habitual, the Immemorial, and the Eternal Return

2008 ◽  
Vol 2 (2) ◽  
pp. 155-177 ◽  
Author(s):  
Eugene Brently Young
Keyword(s):  
Eternal Return

Eternal return is the paradox that accounts for the interplay between difference and repetition, a dynamic at the heart of Deleuze's philosophy, and Blanchot's approach to this paradox, even and especially through what it elides, further illuminates it. Deleuze draws on Blanchot's characterisations of difference, forgetting, and the unlivable to depict the ‘sense’ produced via eternal return, which, for Blanchot, is where repetition implicates or ‘carries’ pure difference. However, for Deleuze, difference and the unlivable are also developed by the living repetition or ‘contraction’ of habit, which results in his distinctive characterization of ‘force’, ‘levity’, and sense in eternal return.

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