Differentiation of Colletotrichum gloeosporioides isolates by using total proteins and esterase electrophoretic patterns and extracellular enzymes production

Isolates of Colletotrichum gloeosporioides (ISO-1, ISO-2, ISO-3, ISO-4, ISO-5 and ISO-6), the causal agent of anthracnose disease on mango fruits, were characterized by electrophoretic patterns of total proteins and esterase in polyacrylamida gel, and also, by production of extracellular enzymes on specific solid substrate. The electrophoretic analysis showed variation in number, intensity of coloration and position of the bands in the gel at each studied system tested. In contrast to the monomorphic behavior to total proteins, high esterase polymorfism was observed indicating difference among isolates. All isolates showed the activity of extracellular enzymes such as amylase, lipase, and protease with some variation among them. The proteolitic activity seemed to be more accentuated than the two other enzymes studied.

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Morphological and Molecular Identification of the Causal Agent of Anthracnose Disease of Avocado in Kenya

International Journal of Microbiology ◽

10.1155/2018/4568520 ◽

2018 ◽

Vol 2018 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

S. K. Kimaru ◽

E. Monda ◽

R. C. Cheruiyot ◽

J. Mbaka ◽

A. Alakonya

Keyword(s):

Colletotrichum Gloeosporioides ◽

Causal Agent ◽

Economic Losses ◽

Lower Side ◽

Cultural Characteristics ◽

Anthracnose Disease ◽

Pestalotiopsis Microspora ◽

Black Spots ◽

Postharvest Rot ◽

Growing Region

Anthracnose disease of avocado contributes to a huge loss of avocado fruits due to postharvest rot in Kenya. The causal agent of this disease has not been clear but presumed to beColletotrichum gloeosporioidesas reported in other regions where avocado is grown. The fungus mainly infects fruits causing symptoms such as small blackish spots, “pepper spots,” and black spots with raised margin which coalesce as infection progresses. Due to economic losses associated with the disease and emerging information of other species of fungi as causal agents of the disease, this study was aimed at identifying causal agent(s) of the disease. A total of 80 fungal isolates were collected from diseased avocado fruits in Murang’a County, the main avocado growing region in Kenya. Forty-six isolates were morphologically identified asColletotrichumspp. based on their cultural characteristics, mainly whitish, greyish, and creamish colour and cottony/velvety mycelia on the top side of the culture and greyish cream with concentric zonation on the reverse side. Their spores were straight with rounded end and nonseptate. Thirty-four isolates were identified asPestalotiopsisspp. based on their cultural characteristics: whitish grey mycelium with black fruiting structure on the upper side and greyish black one on the lower side and septate spores with 3-4 septa and 2 or 3 appendages at one end. Further molecular studies using ITS indicatedColletotrichum gloeosporioides,Colletotrichum boninense, andPestalotiopsis microsporaas the causal agents of anthracnose disease in avocado. However, with this being the first report, there is a need to conduct further studies to establish whether there is coinfection or any interaction thereof.

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In vitro Screening of 75 Essential Oils Against Colletotrichum gloeosporioides: A Causal Agent of Anthracnose Disease of Mango

International Journal of Fruit Science ◽

10.1080/15538362.2017.1377666 ◽

2017 ◽

Vol 18 (1) ◽

pp. 1-13 ◽

Cited By ~ 9

Author(s):

Vijya P. Rabari ◽

Kiran S. Chudashama ◽

Vrinda S. Thaker

Keyword(s):

Essential Oils ◽

Colletotrichum Gloeosporioides ◽

Causal Agent ◽

In Vitro Screening ◽

Anthracnose Disease

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In Vitro Screening of Fungicides and Plant Extracts Against Colletotrichum Gloeosporioides (Penz.) Sacc. the Causal Agent of Anthracnose Disease of Rauwolfia Serpentina (L.) Benth Ex Kurz

Journal of the Asiatic Society of Bangladesh Science ◽

10.3329/jasbs.v45i1.46568 ◽

2019 ◽

Vol 45 (1) ◽

pp. 35-43

Author(s):

Zuhra Yasmin ◽

Shamim Shamsi

Keyword(s):

Growth Inhibition ◽

Plant Extracts ◽

Azadirachta Indica ◽

Moringa Oleifera ◽

Colletotrichum Gloeosporioides ◽

Psidium Guajava ◽

Causal Agent ◽

Citrus Limon ◽

Anthracnose Disease ◽

Rauwolfia Serpentina

Ten fungicides viz., Amcogin 50 WP, Autostin 50 WDG, Capvit 77 WP, Dithane M 45, Greengel 72 WP, Haybit 80 WG, Oxivit 40 SC, Ridomil Gold MZ, Sulcox 50 WP and Tilt 250 EC at 100, 200, 300, 400 and 500 ppm concentrations were tested against, Colletotrichum gloeosporioides (Penz.) Sacc., the causal agent of anthracnose disease of Rauwolfia serpentina (L.) Benth ex Kurz following poisoned food technique. Amcogin, Autostin and Tilt showed complete growth inhibition of C. gloeosporioides at 100 ppm concentration. Ethanol extracts of ten plants viz., Adhatoda vesica, Azadirachta indica, Citrus limon, Curcuma longa, Lantana camara, Moringa oleifera, Ocimum sanctum, Psidium guajava, Thuja orientalis and Vitex negundo were selected to evaluate their efficacy at 5, 10, 15 and 20% concentrations against the same pathogenic fungus. Out of the ten plant extracts, Azadirachta indica, Citrus limon, Moringa oleifera and Psidium guajava showed complete radial growth inhibition of C. gloeosporioides at 20% concentration. Amcogin, Autostin, and Tilt are suggested as best inhibiting chemical fungicides for C. gloeosporioides. Extracts of A. indica, C. limon, M. oleifera and Psidium guajava were also found to be superior to other plant extracts tested in controlling the test pathogen. Asiat. Soc. Bangladesh, Sci. 45(1): 35-43, June 2019

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Colletotrichum Gloeosporioides Species Complex: Pathogen Causing Anthracnose, Gummosis and Die-Back Diseases of Cashew (Anacardium Occidentale L.) In Ghana

European Journal of Agriculture and Food Sciences ◽

10.24018/ejfood.2020.2.6.146 ◽

2020 ◽

Vol 2 (6) ◽

Author(s):

A. Muntala ◽

P. M. Norshie ◽

K. G. Santo ◽

C. K. S. Saba

Keyword(s):

Species Complex ◽

Colletotrichum Gloeosporioides ◽

Potato Dextrose Agar ◽

Causal Agent ◽

Anacardium Occidentale ◽

Causative Organism ◽

Pathogenicity Test ◽

Small Water ◽

Colletotrichum Gloeosporioides Species Complex

A survey was conducted in twenty-five cashew (Anacardium occidentale) orchards in five communities in the Dormaa-Central Municipality of Bono Region of Ghana to assess the incidence and severity of anthracnose, gummosis and die-back diseases on cashew. Cashew diseased samples of leaves, stem, inflorescences, twigs, flowers, nuts and apples showing symptoms (e. g. small, water-soaked, circular or irregular yellow, dark or brown spots or lesions on leaves, fruits and flowers, sunken surface, especially on the apples, blight, gum exudates) were collected for isolation of presumptive causative organism. The pathogen was isolated after disinfecting the excised diseased pieces in 70% ethanol, plated on potato dextrose agar (PDA) and incubated at 28 oC for 3 to 7 days. The identity of the putative pathogen was morphologically and culturally confirmed as belonging to Colletotrichum gloeosporioides species complex using standard mycological identification protocols. The pathogen had varied conidia sizes of between 9-15 up to 20 μm in length and diameter of 3-6 μm. The conidia were straight and cylindrically shaped with rounded or obtuse ends. The septate mycelium was whitish-grey, velvety and cotton-like in appearance from the top. The results confirmed the presence of the pathogen in the orchards with incidence ranging from 6.9% and 14.0% for gummosis and averaged 22.9% for anthracnose infected orchards. The result of the pathogenicity test confirmed the isolates to be pathogenic on inoculated cashew seedlings and were consistently re-isolated, thereby establishing the pathogen as the true causal agent of the said diseases in cashew trees and thus completed the Koch’s postulate.

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