In vitro Screening of 75 Essential Oils Against Colletotrichum gloeosporioides: A Causal Agent of Anthracnose Disease of Mango

Isolates of Colletotrichum gloeosporioides (ISO-1, ISO-2, ISO-3, ISO-4, ISO-5 and ISO-6), the causal agent of anthracnose disease on mango fruits, were characterized by electrophoretic patterns of total proteins and esterase in polyacrylamida gel, and also, by production of extracellular enzymes on specific solid substrate. The electrophoretic analysis showed variation in number, intensity of coloration and position of the bands in the gel at each studied system tested. In contrast to the monomorphic behavior to total proteins, high esterase polymorfism was observed indicating difference among isolates. All isolates showed the activity of extracellular enzymes such as amylase, lipase, and protease with some variation among them. The proteolitic activity seemed to be more accentuated than the two other enzymes studied.

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Control of Anthracnose Disease (Colletotrichum gloeosporioides) Using Nano Chitosan Hydrolyzed by Chitinase Derived from Burkholderia cepacia Isolate E76

Jurnal AgroBiogen ◽

10.21082/jbio.v13n2.2017.p111-122 ◽

2018 ◽

Vol 13 (2) ◽

pp. 111 ◽

Cited By ~ 2

Author(s):

Yadi Suryadi ◽

Tri Puji Priyatno ◽

I Made Samudra ◽

Dwi Ningsih Susilowati ◽

Tuti Septi Sriharyani ◽

...

Keyword(s):

Burkholderia Cepacia ◽

Colletotrichum Gloeosporioides ◽

Sodium Tripolyphosphate ◽

Average Particle Size ◽

Chitosan Nanoparticles ◽

Average Particle ◽

Coating Application ◽

Anthracnose Disease

<p>Anthracnose (Colletotrichum gloeosporioides) is one of the important diseases of fruit crops that need to be controlled. This study was aimed to obtain the best formula of hydrolyzed nano chitosan and its potensial in controlling anthracnose. The hydrolyzed chitosan was prepared using chitinase enzyme extracted from Burkholderia cepacia isolate E76. Chitosan nanoparticles were synthesized using ionic gelation method by reacting hydrolyzed chitosan (0.2%) with Sodium tripolyphosphate (STPP) (0.1%) as cross-linking agent using 30&ndash;60 minutes stirring condition. The bioactivity of the nano chitosan formula was tested to C. gloeosporioides under in vitro and in vivo assays. The specific enzymatic activity of the purified chitinase was higher (0.19 U/mg) than that of crude enzyme (supernatant) with the purity increased by 3.8 times. Of the four formula tested, Formula A (hydrolyzed chitosan to STPP volume ratio of 5 : 1 with 60 minutes stirring condition) was found good in terms of physical characteristic of the particle. The formula nano chitosan particle had the spherical-like shape with an average particle size of 126.2+3.8 nm, polydispersity index (PI) of 0.4+0.02, and zeta potential (ZP) value of 27.8+0.2 mV. Nano chitosan had an inhibitory activity to C. gloeosporioides in vitro up to 85.7%. Moreover, it could inhibit 61.2% of C. gloeosporioides spores germination. It was shown that nano chitosan was also effective to reduce anthracnose disease severity in vivo when applied as a preventive measure on chili and papaya fruits. This study could be used as a reference for further fruit coating application using nano chitosan as a promising postharvest biocontrol agent to C. gloeosporioides.</p>

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EVALUATION OF DIFFERENT ESSENTIAL OILS AND BIO CONTROL AGENTS AGAINST ALTERNARIA ALTERNATA THE CAUSAL AGENT OF FRUIT ROT OF JUJUBE

Journal of Applied Research in Plant Sciences ◽

10.38211/joarps.2020.1.1.1 ◽

2020 ◽

Vol 1 (1) ◽

pp. 1-8

Author(s):

Keyword(s):

Growth Inhibition ◽

Essential Oils ◽

Alternaria Alternata ◽

Colony Growth ◽

Causal Agent ◽

Biocontrol Agents ◽

Fruit Rot ◽

Pathogenicity Test ◽

Neem Oil

This study was carried out on the evaluation of different essential oils and biocontrol agents against Alternaria alternata the causal agent of fruit rot. For the pathogenicity test against A. alternata was performed through cut and injection inoculation methods. The antifungal potential of different essential oils like turpentine, laung, neem and castus root was carried out at different doses i.e. 5, 10 and 15% by food poisoned method to find out the effective and suitable oil for the growth inhibition of A. alternata and evaluate the effect of some biocontrol agents on growth inhibition of A. alternata. The findings of this investigation stated that cut method of inoculation showed higher percentage (2.60) of rotting as compared to injection method of inoculation (2.35). Minimum colony growth of A. alternata (31.60, 21.25 and 15.16%) was examined under Laung oil at the dosage of 5, 10 and 15% followed by Neem oil (42.60, 31.60 and 21.30%), respectively. Maximum colony growth of A. alternata (62.71, 52.40 and 41.75%) was observed under Castus root oil at the dosage of 5, 10 and 15%. Zero growth of target pathogen was examined under Turpentine at 5, 10 and 15%. Under control the A. alternata showed (90 mm) colony growth. Minimum linear colony growth of A. alternata was observed for Hypoxylon Sp1 (50.31%), followed by Neurospora spp. (52.97%), Lasiodiplodia theobromae (54.7%), Chactomium subaffine (57.07%) and Fusarium sp. (65.4%). Maximum mycelial colony growth (90%) was recorded in control. Based on present investigation, Similarly, for controlling the linear colony growth of A. alternata under in vitro conditions Turpentine oil ranked 1st, Laung oil ranked 2nd, Neem oil ranked 3rd, Castus root oil ranked 4th.

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In vitro effect of essential oils on Mushroom pathogen Mycogone perniciosa causal agent of Wet Bubble Disease of White Button Mushroom.

Indian Journal Of Applied Research ◽

10.15373/2249555x/apr2014/152 ◽

2011 ◽

Vol 4 (4) ◽

pp. 482-484 ◽

Cited By ~ 1

Author(s):

Aprna Sabharwal ◽

◽

S. Kapoor S. Kapoor

Keyword(s):

Essential Oils ◽

Causal Agent ◽

Button Mushroom ◽

White Button Mushroom ◽

Vitro Effect

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In vitro Screening of Antibacterial and Antioxidant Activities of Essential Oils from Four Moroccan Medicinal Plants

Microbiology Research Journal International ◽

10.9734/mrji/2017/30073 ◽

2017 ◽

Vol 18 (4) ◽

pp. 1-10 ◽

Cited By ~ 10

Author(s):

Abdelhakim Bouyahya ◽

Youssef Bakri ◽

Abdeslam Et-Touys ◽

Ahmed Talbaoui ◽

Aya Khouchlaa ◽

...

Keyword(s):

Medicinal Plants ◽

Essential Oils ◽

Antioxidant Activities ◽

In Vitro Screening

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Morphological and Molecular Identification of the Causal Agent of Anthracnose Disease of Avocado in Kenya

International Journal of Microbiology ◽

10.1155/2018/4568520 ◽

2018 ◽

Vol 2018 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

S. K. Kimaru ◽

E. Monda ◽

R. C. Cheruiyot ◽

J. Mbaka ◽

A. Alakonya

Keyword(s):

Colletotrichum Gloeosporioides ◽

Causal Agent ◽

Economic Losses ◽

Lower Side ◽

Cultural Characteristics ◽

Anthracnose Disease ◽

Pestalotiopsis Microspora ◽

Black Spots ◽

Postharvest Rot ◽

Growing Region

Anthracnose disease of avocado contributes to a huge loss of avocado fruits due to postharvest rot in Kenya. The causal agent of this disease has not been clear but presumed to beColletotrichum gloeosporioidesas reported in other regions where avocado is grown. The fungus mainly infects fruits causing symptoms such as small blackish spots, “pepper spots,” and black spots with raised margin which coalesce as infection progresses. Due to economic losses associated with the disease and emerging information of other species of fungi as causal agents of the disease, this study was aimed at identifying causal agent(s) of the disease. A total of 80 fungal isolates were collected from diseased avocado fruits in Murang’a County, the main avocado growing region in Kenya. Forty-six isolates were morphologically identified asColletotrichumspp. based on their cultural characteristics, mainly whitish, greyish, and creamish colour and cottony/velvety mycelia on the top side of the culture and greyish cream with concentric zonation on the reverse side. Their spores were straight with rounded end and nonseptate. Thirty-four isolates were identified asPestalotiopsisspp. based on their cultural characteristics: whitish grey mycelium with black fruiting structure on the upper side and greyish black one on the lower side and septate spores with 3-4 septa and 2 or 3 appendages at one end. Further molecular studies using ITS indicatedColletotrichum gloeosporioides,Colletotrichum boninense, andPestalotiopsis microsporaas the causal agents of anthracnose disease in avocado. However, with this being the first report, there is a need to conduct further studies to establish whether there is coinfection or any interaction thereof.

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