scholarly journals Co-infection by Tritrichomonas foetus and Pentatrichomonas hominis in asymptomatic cats

2015 ◽  
Vol 35 (12) ◽  
pp. 980-988 ◽  
Author(s):  
Caroline Spitz dos Santos ◽  
Vera Lúcia Teixeira de Jesus ◽  
Douglas McIntosh ◽  
Bruno Pereira Berto ◽  
Carlos Wilson Gomes Lopes
Keyword(s):  
Molecular Analysis ◽  
Morphological Analysis ◽  
Correct Diagnosis ◽  
Diagnostic Techniques ◽  
Tritrichomonas Foetus ◽  
Specific Primers ◽  
Transmission Electron ◽  
Appropriate Treatment ◽  
Simultaneous Infection ◽  
Pentatrichomonas Hominis

Abstract: Tritrichomonas foetus, a parasite well known for its significance as a venereally transmitted pathogen in cattle, has been identified as a cause of chronic large bowel diarrhea in domestic cats in many countries of the world. In Brazil, several studies on the diagnosis of bovine trichomoniasis have been performed, but until now, no study was made regarding feline trichomoniasis. Thus, this is the first study to report the occurrence of T. foetus and Pentatrichomonas hominis in cats using morphological and molecular analysis. Feces from 77 cats were examined, four of which (5.2%) were positive for the presence of parabasalids. Morphological analysis of stained smears revealed piriform trophozoites showing the three anterior flagella, elongated nucleus and axostyle ending abruptly in fillet, characteristic of T. foetus. In scanning and transmission electron microscopy, identification characters similar to those previously reported for T. foetus were observed. The cultures containing trophozoites were submitted for molecular analysis, which resulted positive for T. foetus DNA using specific primers (TFR3 and TFR4), and all samples were positive and subjected to sequencing in which they showed 99.7-100% similarity with another isolate sequencing of T. foetus (JX960422). Although no trophozoite with consistent morphology of P. hominis has been visualized in the samples, differential diagnosis was performed using specific primers for P. hominis (TH3 and TH5) amplicon. In three of the four samples (3.89%) sequencing revealed 100% similarity when compared with another sequence of P. hominis deposited in Genbank (KC623939). Therefore, the present study revealed through the diagnostic techniques employed the simultaneous infection by T. foetus and P. hominis in the feces of cats. However, it was necessary to use more than one technique for the diagnosis of the co-infection. These results demonstrate the importance of a correct diagnosis to allow an appropriate treatment by the veterinarian.

scholarly journals Renal Tumors of Childhood—A Histopathologic Pattern-Based Diagnostic Approach

Cancers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 729 ◽  
Author(s):  
Ariadne H.A.G. Ooms ◽  
Gordan M. Vujanić ◽  
Ellen D’Hooghe ◽  
Paola Collini ◽  
Aurore L’Herminé-Coulomb ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Molecular Analysis ◽  
Pediatric Oncology ◽  
Correct Diagnosis ◽  
Renal Tumors ◽  
Pediatric Tumors ◽  
Cell Type ◽  
Appropriate Treatment ◽  
Histologic Pattern

Renal tumors comprise approximately 7% of all malignant pediatric tumors. This is a highly heterogeneous group of tumors, each with its own therapeutic management, outcome, and association with germline predispositions. Histopathology is the key in establishing the correct diagnosis, and therefore pathologists with expertise in pediatric oncology are needed for dealing with these rare tumors. While each tumor shows different histologic features, they do have considerable overlap in cell type and histologic pattern, making the diagnosis difficult to establish, if based on routine histology alone. To this end, ancillary techniques, such as immunohistochemistry and molecular analysis, can be of great importance for the correct diagnosis, resulting in appropriate treatment. To use ancillary techniques cost-effectively, we propose a pattern-based approach and provide recommendations to aid in deciding which panel of antibodies, supplemented by molecular characterization of a subset of genes, are required.

scholarly journals Mucoadhesive Nanoparticles for Drug Delivery to the Anterior Eye

Nanomaterials ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1400
Author(s):  
Nicole Mangiacotte ◽  
Graeme Prosperi-Porta ◽  
Lina Liu ◽  
Megan Dodd ◽  
Heather Sheardown
Keyword(s):  
Morphological Analysis ◽  
Phenylboronic Acid ◽  
Spherical Particles ◽  
Target Tissue ◽  
Hydroxyethyl Methacrylate ◽  
Potential Analysis ◽  
Transmission Electron ◽  
Nanoparticle System ◽  
Zeta Potential Analysis

While the use of topical drops for the delivery of drugs to the anterior of the eye is well accepted, it is far from efficient with as little as 5% of the drug instilled on the eye actually reaching the target tissue. The ability to prolong the residence time on the eye is desirable. Based on the acceptability of 2-hydroxyethyl methacrylate based polymers in contact lens applications, the current work focuses on the development of a poly(2-hydroxyethyl methacrylate (HEMA)) nanoparticle system. The particles were modified to allow for degradation and to permit mucoadhesion. Size and morphological analysis of the final polymer products showed that nano-sized, spherical particles were produced. FTIR spectra demonstrated that the nanoparticles comprised poly(HEMA) and that 3-(acrylamido)phenylboronic acid (3AAPBA), as a mucoadhesive, was successfully incorporated. Degradation of nanoparticles containing N,N′-bis(acryloyl)cystamine (BAC) after incubation with DL-dithiothreitol (DTT) was confirmed by a decrease in turbidity and through transmission electron microscopy (TEM). Nanoparticle mucoadhesion was shown through an in-vitro zeta potential analysis.

scholarly journals Prenatal diagnosis of 7 cases with uniparental disomy by utilization of single nucleotide polymorphism array

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lili Zhou ◽  
Zhaoke Zheng ◽  
Yunzhi Xu ◽  
Xiaoxiao Lv ◽  
Chenyang Xu ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Prenatal Diagnosis ◽  
Molecular Analysis ◽  
Correct Diagnosis ◽  
Snp Array ◽  
Uniparental Disomy ◽  
Chromosome 1 ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Rescue Mechanism

Abstract Background The phenotypes of uniparental disomy (UPD) are variable, which may either have no clinical impact, lead to clinical signs and symptoms. Molecular analysis is essential for making a correct diagnosis. This study involved a retrospective analysis of 4512 prenatal diagnosis samples and explored the molecular characteristics and prenatal phenotypes of UPD using a single nucleotide polymorphism (SNP) array. Results Out of the 4512 samples, a total of seven cases of UPD were detected with an overall frequency of 0.16%. Among the seven cases of UPD, two cases are associated with chromosomal aberrations (2/7), four cases (4/7) had abnormal ultrasonographic findings. One case presented with iso-UPD (14), and two case presented with mixed hetero/iso-UPD (15), which were confirmed by Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) as maternal UPD (15) associated with Prader-Willi syndrome (PWS). Four cases had iso-UPD for chromosome 1, 3, 14, and 16, respectively; this is consistent with the monosomy rescue mechanism. Another three cases presented with mixed hetero/isodisomy were consistent with a trisomy rescue mechanism. Conclusion The prenatal phenotypes of UPD are variable and molecular analysis is essential for making a correct diagnosis and genetic counselling of UPD. The SNP array is a useful genetic test in prenatal diagnosis cases with UPD.

scholarly journals Type Va extrahepatic bile duct duplication: a case report

2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Bharat Mani Banjade ◽  
Ashish Rajbhandari ◽  
Rabin Koirala ◽  
Tuhin Shah ◽  
Chitra Lal Bhattachan
Keyword(s):  
Case Report ◽  
Bile Duct ◽  
Extrahepatic Bile Duct ◽  
Correct Diagnosis ◽  
Diagnostic Techniques ◽  
Case Presentation ◽  
Double Common Bile Duct ◽  
Unusual Variant ◽  
Rural Nepal ◽  
Calculous Cholecystitis

Abstract Background Extrahepatic bile duct duplication is an extremely rare congenital anomaly in which two common bile ducts exist. There are five different types of this anomaly and we present an unusual variant of duplication of an extrahepatic biliary system of type Va variety. Case presentation This case report describes a 63-year-old women from rural Nepal who presented with type Va of duplicated extrahepatic bile duct, with chronic calculous cholecystitis and choledocholithiasis. She was managed with cholecystectomy with hepatic ductoplasty and hepaticojejunostomy. Conclusion A rare case of double common bile duct (type Va) complicated by choledocholithiasis, cholangitis, and chronic cholecystitis is reported here. Rare cases are sometimes overlooked by modern diagnostic techniques. Correct diagnosis helps appropriate surgical intervention.

scholarly journals An intrasellar germinoma with normal tumor marker concentrations mimicking primary lymphocytic hypophysitis

2013 ◽  
Vol 57 (7) ◽  
pp. 566-570 ◽  
Author(s):  
Mariana F. Guzzo ◽  
Cristina B. Formiga Bueno ◽  
Thiago T. Amancio ◽  
Sergio Rosemberg ◽  
Cleonice Bueno ◽  
...  
Keyword(s):  
Clinical Laboratory ◽  
Correct Diagnosis ◽  
Careful Analysis ◽  
Malignant Neoplasms ◽  
Pituitary Hormone ◽  
Appropriate Treatment ◽  
Suprasellar Region ◽  
Neoplastic Lesions ◽  
Posterior Pituitary Hormone ◽  
Pituitary Stalk Thickening

Intracranial germinomas (GE) are malignant neoplasms most commonly found in the suprasellar region, which may cause anterior and particularly posterior pituitary hormone deficits with central diabetes insipidus (DI). Differential diagnosis of pituitary stalk thickening includes granulomatous, inflammatory, infectious, and neoplastic lesions. Although careful analysis of clinical, laboratory, and imaging findings may facilitate the diagnosis, transsphenoidal biopsy is indicated to confirm the disease, as the correct diagnosis directs the appropriate treatment.

Morphological analysis of interstitial cells in murine epididymis using light microscopy and transmission electron microscopy

2021 ◽  
Vol 123 (6) ◽  
pp. 151761
Author(s):  
Tasuku Hiroshige ◽  
Kei-Ichiro Uemura ◽  
Shingo Hirashima ◽  
Kiyosato Hino ◽  
Akinobu Togo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Morphological Analysis ◽  
Interstitial Cells ◽  
Transmission Electron

scholarly journals Recurrent leiomyomatosis peritonealis disseminate: a case report

2021 ◽  
Vol 55 (2) ◽  
pp. 160-164
Author(s):  
Yaw B. Mensah ◽  
Lawrence Buadi ◽  
Afua Abrahams ◽  
Andrea A. Y. Appau ◽  
Kwadwo Mensah
Keyword(s):  
Correct Diagnosis ◽  
Reproductive Age ◽  
Gnrh Analogue ◽  
Ultrasound Guided ◽  
Pelvic Ultrasound ◽  
Women Of Reproductive Age ◽  
Unusual Condition ◽  
Appropriate Treatment ◽  
Leiomyomatosis Peritonealis Disseminata ◽  
Histopathologic Analysis

Leiomyomatosis peritonealis disseminata (LPD), a rare and unusual condition affecting mainly women of reproductive age, causes peritoneal and subperitoneal nodules formed by smooth muscle. Very few cases have been diagnosed since the disease was first described. We present a 42year old female who was managed for infertility and uterine myomata at a Municipal hospital in Ghana. Following a pelvic ultrasound diagnosis of multiple uterine myomata the patient was booked for myomectomy. At surgery to remove her myomata, the patient was found to have several peritoneal nodules some of which were attached to peritoneum, omentum and the surface of bowel loops in addition to a uterine myoma. The disease has since recurred twice after two laparotomies. The diagnosis was made by histopathology of ultrasound-guided biopsy of the nodules, and she has since been on GnRH analogue treatment. LPD simulates peritoneal carcinomatosis; thus, a good history, clinical evaluation, radiological imaging, and histopathologic analysis must be accurately diagnosed. Surgeons’ and Radiologists’ knowledge of the condition is fundamental to ensuring correct diagnosis and appropriate treatment and to minimising the probability of malignant transformation.

scholarly journals Importance of Laparoscopy to Solve the Diagnostic Dilemma of Abdominal Tuberculosis

KYAMC Journal ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 14-17
Author(s):  
Md Saiful Islam ◽  
Md Masudar Rahman ◽  
M Fardil Hossain Faisal ◽  
Md Alamgir Jalil Pramanik ◽  
Muhammad Abdur Rouf
Keyword(s):  
Minimally Invasive ◽  
Diagnostic Laparoscopy ◽  
Abdominal Cavity ◽  
Correct Diagnosis ◽  
Abdominal Tuberculosis ◽  
Diagnostic Dilemma ◽  
Peritoneal Tissue ◽  
Appropriate Treatment ◽  
Liver Surface ◽  
Minimally Invasive Access

Background: Diagnosis of abdominal tuberculosis as well as histopathological confirmation is difficult because of suboptimal access to the intraperitoneal pathology. Laparoscopy provides minimally invasive access to the peritoneal cavity and materials can be collected for confirmation of diagnosis. Objectives: To study the importance of laparoscopy as a tool for the diagnosis of abdominal tuberculosis and initiation of appropriate treatment without delay. Materials & Methods: In this study 25 patients with suspected abdominal tuberculosis were selected within the period of May, 2014 to October, 2014. Diagnostic laparoscopy performed on all patients with biopsy of tissue from accessible sites. Results: Diagnostic laparoscopy with biopsy confirmed the diagnosis in 24 (96%) patients, 23 of these patients (96%) had nodules at different site of abdominal cavity and 19 of these patients (76%) had ascites. In two cases there were nodules over liver surface; biopsy was taken also from both liver nodules. One nodule revealed fibrosis and another nodule revealed tuberculosis. Conclusion: Imaging and culture of ascitic fluid may fail to confirm or exclude abdominal tuberculosis in clinically suspected cases. Laparoscopy with peritoneal tissue biopsy provided rapid and correct diagnosis of abdominal tuberculosis and should be performed early in suspected cases. KYAMC Journal.2021;12(01): 14-17

scholarly journals Morphological and molecular identifications of three native arbuscular mycorrhizal fungi isolated from the rhizosphere of Elaeis guineensis and Jatropha curcas in Indonesia

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
Maria Viva Rini ◽  
Fitri Yelli ◽  
Darwin Leonardo Tambunan ◽  
Inggar Damayanti
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Molecular Analysis ◽  
Jatropha Curcas ◽  
Mycorrhizal Fungi ◽  
Morphological Analysis ◽  
Agricultural Land ◽  
Elaeis Guineensis ◽  
Arbuscular Mycorrhizal ◽  
Fungal Colonization ◽  
Morphological Studies

Abstract. Rini MV, Yelli F, Tambunan DL, Damayanti I. 2021. Morphological and molecular identifications of three native arbuscular mycorrhizal fungi isolated from the rhizosphere of Elaeis guineensis and Jatropha curcas in Indonesia. Biodiversitas 22: 4940-4947. Molecular analysis has been widely used to provide more accurate identification within arbuscular mycorrhizal fungi (AMF) species than identification based on morphology. However, morphological analysis is essential for a basic preliminary of classification studies. Therefore, a study is needed to complete the identification of AMF isolates through morphological and molecular analyses. This research used three AMF isolates, namely MV 5, MV 17, and MV 18, which were isolated from Indonesian agricultural land. Spore-based taxonomy (shape, size, color, ornamentation, PVLG, and Melzer’s reaction) and fungal colonization on roots of maize trap plants were employed for the morphological studies. AMF species identification was performed using molecular analysis through nested-Polymerase Chain Reaction (PCR) to amplify a fragment of SSU rRNA followed by sequencing and phylogenetic tree construction. Morphological analysis showed that MV 5 had spores borne from the neck of the sporiferous saccule, MV 17 was found to have a bulbous suspensor without a germination shield, and MV 18 had spores borne from subtending hyphae. The SSUR rRNA analysis revealed that MV 5, MV 15, and MV 18 were identified as Acaulospora longula, Gigaspora margarita, and Glomus etunicatum, respectively. Both morphological and molecular methods demonstrated reliable and consistent results that complement AMF taxonomy studies.

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