Regulation of the expression of NADP-malic enzyme by UV-B, red and far-red light in maize seedlings

The induction of NADP-malic enzyme (NADP-ME) in maize (Zea mays) etiolated seedlings as a function of intensity and duration of exposure to UV-B radiation was investigated by measuring changes in activity and protein, as well as by levels of NADP-ME gene transcripts. Exposures to UV radiation from 2 to 60 min including photon flux densities of UV-B of 0.5–2 mmol quanta m-2 s-1 which are well below levels which occur naturally under full sunlight, caused an increase in Me gene mRNA and NADP-ME protein, as well as in NADP-ME activity. A similar dosage of UV-A radiation was ineffective. A low level of red light (10 µmol quanta m-2 s-1) for only 5 min was also effective in inducing increases in NADP-ME activity equivalent to that with UV-B radiation. A 5 min exposure to far red light (100 µmol quanta m-2 s-1) following UV-B or red light treatment largely reversed the induction of NADP-ME, and this effect of far-red could be eliminated by further treatment with UV-B or red light. These results indicate that physiological levels of UV-B radiation have a positive effect on induction of the synthesis of a photosynthetic enzyme which is involved in C4 photosynthesis.

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The effect of red and far-red light on carotenoid synthesis by etiolated maize seedlings

Phytochemistry ◽

10.1016/s0031-9422(00)88002-4 ◽

1962 ◽

Vol 1 (2) ◽

pp. 67-72 ◽

Cited By ~ 40

Author(s):

Renee Z. Cohen ◽

T.W. Goodwin

Keyword(s):

Red Light ◽

Maize Seedlings ◽

Carotenoid Synthesis

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Red Light-inhibited Mesocotyl Elongation in Maize Seedlings

PLANT PHYSIOLOGY ◽

10.1104/pp.61.4.534 ◽

1978 ◽

Vol 61 (4) ◽

pp. 534-537 ◽

Cited By ~ 22

Author(s):

Larry N. Vanderhoef ◽

Winslow R. Briggs

Keyword(s):

Red Light ◽

Maize Seedlings ◽

Mesocotyl Elongation

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Maize AUXIN-BINDING PROTEIN 1 and AUXIN-BINDING PROTEIN 4 impact on leaf growth, elongation, and seedling responsiveness to auxin and light

Botany ◽

10.1139/b2012-071 ◽

2012 ◽

Vol 90 (10) ◽

pp. 990-1006 ◽

Cited By ~ 3

Author(s):

Dejana Jurišić-Knežev ◽

Mária Čudejková ◽

David Zalabák ◽

Marta Hlobilová ◽

Jakub Rolčík ◽

...

Keyword(s):

Binding Protein ◽

Leaf Growth ◽

Red Light ◽

Maize Seedlings ◽

Exogenous Auxin ◽

Auxin Binding ◽

Auxin Binding Protein ◽

Auxin Binding Proteins ◽

The Relationship

In maize, at least five auxin-binding proteins (ABPs) have been identified, yet their functions remain unclear. The present study reports the use of maize abp1, abp4, and abp1abp4 mutants to investigate the role of ABPs during maize growth and development. Single and double abp mutant plants grown in a greenhouse differ from the wild type (WT) in their leaf declination and leaf blade growth. The effect of the dark (D), blue light (BL), red light (RL), and exogenous auxin on the development of mutant seedlings was also studied. Relative to WT, etiolated mutant seedlings were shorter and showed a reduced responsiveness to exogenous auxin. In BL or RL, the responsiveness of maize seedlings to auxin was distinctly less than in D. The reducing effect of light on seedling responsiveness to auxin is mediated at least by phytochromes. The suppression of ABP1 and (or) ABP4 led to a distinct accumulation of free indole-3-acetic acid (IAA) in etiolated and light-grown seedling organs. We concluded that ABP1 and ABP4 participate in the growth of maize seedlings, mediate seedling responses to auxin, and interact with light signaling pathway(s). We also deduce a functional interaction between ABP1 and ABP4, which is that the relationship between them is light-, organ- and response-dependent.

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The influence of drought stress on the development of maize seedlings and the dynamics of activity of enzymes in their tissues

NATURE AND SCIENCE ◽

10.36719/2707-1146/03/39-42 ◽

2020 ◽

Vol 02 (03) ◽

pp. 39-42

Author(s):

Ziyaddin Mahmud Mammadov ◽

Naila Zahir Aliyeva

Keyword(s):

Drought Stress ◽

Key Words ◽

Malate Dehydrogenase ◽

Malic Enzyme ◽

Maize Seedling ◽

Maize Seedlings ◽

Noticeable Increase ◽

Key Words Maize ◽

Activity Of Enzymes ◽

Glucose 6 Phosphate Dehydrogenase

Investigated the dynamics of the activity of glucose-6-phosphate dehydrogenase (G6PDG, EC 1.1.1.49) and malate dehydrogenase decarboxylating (MDHD, malic- enzyme, EC 1.1.1.40) enzymes that play an important role in the formation of NADPH pool of cells, under drought stress. It has been established that the development of maize seedlings is accompanied by a weakening of the activity of the G6PDH and a noticeable increase in the activity of MDHD. A drought stress causes activation of both of enzymes, in particular G6PGH, both in the root and in the stem tissues of the seedlings. An increase in the concentration is accompanied by the induction of MDHD activity to a greater extent, than that of G6PDH. Key words: maize seedling, drought stress, glucose-6-phosphate dehidrogenase, malate dehydrogenase decarboxylating

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UV-B induction of NADP-malic enzyme in etiolated and green maize seedlings

Plant Cell & Environment ◽

10.1046/j.1365-3040.1998.00240.x ◽

1998 ◽

Vol 21 (1) ◽

pp. 63-70 ◽

Cited By ~ 21

Author(s):

M. F. Drincovich ◽

P. Casati ◽

C. S. Andreo ◽

R. Donahue ◽

G. E. Edwards

Keyword(s):

Malic Enzyme ◽

Maize Seedlings

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Influence of red light on the expression of genes on stomatal formation in maize seedlings

Canadian Journal of Plant Science ◽

10.1139/cjps-2019-0140 ◽

2020 ◽

Vol 100 (3) ◽

pp. 296-303

Author(s):

Tie Dong Liu ◽

Xi Wen Zhang ◽

Yong Xu

Keyword(s):

Blue Light ◽

Stomatal Density ◽

Red Light ◽

Net Photosynthesis ◽

Stomatal Development ◽

Intercellular Signaling ◽

Stomatal Index ◽

Maize Seedlings ◽

Expression Levels ◽

Expression Of Genes

Red light significantly affects the expression of plant photoreceptor genes and influences stomatal development through crosstalk of the constitutive photomorphogenic 1–cryptochrome–phytochrome signaling pathway. When blue light was replaced with red light, the expression levels of ZmCry1, ZmPhyB1, ZmEPF2, and ZmEPFL9 were enhanced, whereas that of ZmCOP1 was restricted. Moreover, the expression levels of ZmSPCH and ZmMUTE were also enhanced, but they were generally lower than those under white light. Consequently, stomatal formation, which was determined by net photosynthesis, stomatal conductance, intercellular CO2 concentration, and transpiration rate, was inhibited through decreased stomatal index and stomatal density. We conclude that red light positively regulates EPFL9 in the intercellular signaling but reduces the positive regulation of blue light on COP1 and epidermal patterning factor 2 in the intracellular and intercellular signaling; therefore, though red light promotes the gene’s function on stomatal development of seedling maize, blue light maybe dominant to red light in seedling stage.

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