scholarly journals Wound Healing Potential of Rhodomyrtus tomentosa and its Bioactive Compounds- Rhodomyrtone

2021 ◽  
pp. 262-273
Author(s):  
Evana Kamarudin ◽  
Hasseri Halim ◽  
Tengku Shahrul Anuar ◽  
Roslinah Hussain
Keyword(s):  
Wound Healing ◽  
Bioactive Compounds ◽  
Human Fibroblast ◽  
Ethanolic Extract ◽  
Fibroblast Cells ◽  
Human Fibroblast Cells ◽  
Healing Agent ◽  
Wound Healing Activity ◽  
Rhodomyrtus Tomentosa

Aims: The present work was aimed to study the phytochemical composition of a crude ethanolic extract of Rhodomyrtus tomentosa [SERT], and the presence of rhodomyrtone and SERT's in vitro wound healing activity. Introduction: Rhodomyrtus tomentosa is native plant to southern and southeastern Asia, India, east to southern China, Taiwan, Philippines, and south to Malaysia. In the traditional Vietnamese, Chinese and Malaysia, all its part, including leaves, roots, buds, and fruits have been used. A need for a new source of wound healing agent is the call for the investigation of the potential of R. tomentosa as the source of health-promoting agent, specifically as a natural wound healing agent. Methodology: SERT was screen for its phytochemicals and the detection of rhodomyrtone using liquid chromatography–mass spectrometry, /Quadrupole time-of-flight [LC-MS/QTOF] analysis. Cell viability, cell proliferation, and migration assay were performed to examine the SERT effect's in vitro wound healing activity on human fibroblast cells [CRL-2522]. Results: The phytochemical study showed the presence of saponins, flavonoids, tannins and steroid in the crude ethanolic extract. The LC-MS analysis of crude ethanolic extract of SERT showed presents of rhodomyrtone which is one of the major compounds in the extract. SERT exhibit proliferative and migratory rate in human fibroblast cells [CRL 2522] in dose-dependent manner, which supports wound healing process. Its bioactive compounds presented wound healing activities at 0.325 up to 2.5 µg/mL. Conclusion: Both SERT and rhodomyrtone portrayed in vitro wound healing activities. Further studies to elucidate the mechanism of action of SERT and rhodomyrtone is recommended.

scholarly journals Grammatophyllum speciosum Ethanolic Extract Promotes Wound Healing in Human Primary Fibroblast Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Saraporn Harikarnpakdee ◽  
Verisa Chowjarean
Keyword(s):  
Wound Healing ◽  
Ethanolic Extract ◽  
Skin Wound ◽  
Total Phenolic ◽  
Fibroblast Cells ◽  
Skin Wound Healing ◽  
Primary Fibroblast ◽  
Human Primary Fibroblast ◽  
Phytochemical Compounds

Grammatophyllum speciosum is a plant in Orchidaceae family which contains a variety of phytochemical compounds that might be beneficial for medicinal use. This study aimed to evaluate the activity of pseudobulb of G. speciosum extract (GSE) in wound healing processes in human primary fibroblast cells along with in vitro antioxidant activity and total phenolic content of GSE. Scratch wound healing assay indicated that GSE was capable of increasing migration rate after 6 and 9 hours of treatment. Besides, the extract was able to scavenge DPPH, ABTS, and superoxide anion radicals indicating the antioxidative property of GSE. This study suggested a novel role of the of pseudobulb extract of G. speciosum as a wound healing enhancer. The results from this study might be beneficial for the development of further novel active compounds for skin wound healing.

scholarly journals Lack of DNA damage induced by fluoride on mouse lymphoma and human fibroblast cells by single cell gel (comet) assay

2006 ◽  
Vol 17 (2) ◽  
pp. 91-94 ◽  
Author(s):  
Daniel Araki Ribeiro ◽  
Patrícia Lepage Alves de Lima ◽  
Mariângela Esther Alencar Marques ◽  
Daisy Maria Fávero Salvadori
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Single Cell ◽  
Human Fibroblast ◽  
Fibroblast Cells ◽  
Potential Health ◽  
Human Fibroblast Cells ◽  
Genotoxicity Tests ◽  
Mouse Lymphoma

Fluoride has widely been used in Dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury on genetic apparatus. Genotoxicity tests constitute an important part of cancer research for risk assessment of potential carcinogens. In this study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Mouse lymphoma and human fibroblast cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 µg/mL for 3 h at 37ºC. The results pointed out that NaF in all tested concentrations did not contribute to DNA damage as depicted by the mean tail moment and tail intensity for both cellular types assessed. These findings are clinically important because they represent a valuable contribution for evaluation of the potential health risk associated with exposure to agents usually used in dental practice.

scholarly journals In vitro cytotoxicity of chemical preservatives on human fibroblast cells

2018 ◽  
Vol 54 (1) ◽  
Author(s):  
Daniel Gonsales Spindola ◽  
Andre Hinsberger ◽  
Valéria Maria de Souza Antunes ◽  
Luis Felipe Gomes Michelin ◽  
Claudia Bincoletto ◽  
...  
Keyword(s):  
Human Fibroblast ◽  
In Vitro Cytotoxicity ◽  
Fibroblast Cells ◽  
Human Fibroblast Cells ◽  
Chemical Preservatives

Differential effects of phorbol ester on the in vitro invasiveness of malignant and non-malignant human fibroblast cells

1990 ◽  
Vol 142 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Rafael Fridman ◽  
Juan Carlos Lacal ◽  
Reuven Reich ◽  
Daniel R. Bonfil ◽  
Chang-Ho Ahn
Keyword(s):  
Phorbol Ester ◽  
Human Fibroblast ◽  
Fibroblast Cells ◽  
Differential Effects ◽  
Human Fibroblast Cells ◽  
In Vitro Invasiveness

scholarly journals Polysaccharides as wound healing agent: a mini review

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 31-37
Author(s):  
J. Rohini ◽  
M.F. Wan Ezumi ◽  
M.S. Rabeta
Keyword(s):  
Wound Healing ◽  
Bioactive Compounds ◽  
Scientific Evidence ◽  
Well Being ◽  
In Vivo Model ◽  
Current Trends ◽  
Healing Agent ◽  
Pharmaceutical Industries

Medicinal eukaryotes, such as plants and fungi, have prompted researchers to conduct extensive studies on their medicinal values for drug discovery. Current trends focus on bioactive compounds of medicinal plants to produce inventions in the medical and health fields. Among many bioactive compounds, polysaccharides attract attention because they are non-toxic and have no side effects. Polysaccharides have been widely used in food and pharmaceutical industries as a secondary ingredient for several decades. This paper reviewed the applications of polysaccharides as wound healing agents. Wounds can affect the patient’s well-being, self-image, working capacity and independence. Research studies on different sources of polysaccharides by in vitro and in vivo model have been investigated. Based on the scientific evidence related to polysaccharides, this work will be a baseline study for future investigations in different fields. All literature was accessed through available electronic databases

scholarly journals The effect of intermediate frequency electromagnetic field at 22 kHz on human fibroblast cells in vitro

2021 ◽  
Author(s):  
Bertalan Pintér ◽  
Zsófia Szilágyi ◽  
Erika Szabó ◽  
Györgyi Kubinyi ◽  
Yves Le Drean ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Human Fibroblast ◽  
Intermediate Frequency ◽  
Fibroblast Cells ◽  
Human Fibroblast Cells

Reactivity trends of cobalt(III) complexes towards various amino acids based on the properties of the amino acid alkyl chains

2020 ◽  
Vol 76 (7) ◽  
pp. 663-672
Author(s):  
Charmaine Arderne ◽  
Kyle Fraser Batchelor ◽  
Bhawna Uprety ◽  
Rahul Chandran ◽  
Heidi Abrahamse
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Human Fibroblast ◽  
Fibroblast Cells ◽  
Normal Human Fibroblast ◽  
Alkyl Chains ◽  
Human Fibroblast Cells ◽  
Vis Spectroscopy ◽  
Normal Human

The reactivity of the cobalt(III) complexes dichlorido[tris(2-aminoethyl)amine]cobalt(III) chloride, [CoCl2(tren)]Cl, and dichlorido(triethylenetetramine)cobalt(III) chloride, [CoCl2(trien)]Cl, towards different amino acids (L-proline, L-asparagine, L-histidine and L-aspartic acid) was explored in detail. This study presents the crystal structures of three amino acidate cobalt(III) complexes, namely, (L-prolinato-κ2 N,O)[tris(2-aminoethyl)amine-κ4 N,N′,N′′,N′′′]cobalt(III) diiodide monohydrate, [Co(C5H8NO2)(C6H18N4)]I2·H2O, I, (L-asparaginato-κ2 N,O)[tris(2-aminoethyl)amine-κ4 N,N′,N′′,N′′′]cobalt(III) chloride perchlorate, [Co(C4H7N2O3)(C6H18N4)](Cl)(ClO4), II, and (L-prolinato-κ2 N,O)(triethylenetetramine-κ4 N,N′,N′′,N′′′)cobalt(III) chloride perchlorate, [Co(C4H7N2O3)(C6H18N4)](Cl)(ClO4), V. The syntheses of the complexes were followed by characterization using UV–Vis spectroscopy of the reaction mixtures and the initial rates of reaction were obtained by calculating the slopes of absorbance versus time plots. The initial rates suggest a stronger reactivity and hence greater affinity of the cobalt(III) complexes towards basic amino acids. The biocompatibility of the complexes was also assessed by evaluating the cytotoxicity of the complexes on cultured normal human fibroblast cells (WS1) in vitro. The compounds were found to be nontoxic after 24 h of incubation at concentrations up to 25 mM.

Thermal Properties, Biodegradability and Cytotoxicity of PLA/Sericin Films

2011 ◽  
Vol 410 ◽  
pp. 86-89
Author(s):  
Suriyan Rakmae ◽  
Nitinat Suppakarn
Keyword(s):  
Thermal Properties ◽  
Human Fibroblast ◽  
Film Surface ◽  
Fibroblast Cells ◽  
Human Fibroblast Cells

In this study, PLA/sericin films at various contents of sericin were prepared. Thermal properties, in vitro degradability and in vitro cytotoxicity of the films were characterized. The results illustrated that the incorporation of sericin into PLA matrix crucially affected thermal properties and biodegradability of the films and also enhanced human fibroblast cells attachment and proliferation on the film surface.

COMPARATIVE EVALUATION OF IN VITRO ANTIMICROBIAL AND IN VIVO WOUND HEALING ACTIVITY OF ETHANOLIC AND ETHYL ACETATE EXTRACTS OF ABUTILON INDICUM ROOT

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (04) ◽  
pp. 21-31
Author(s):  
K Ravishankar ◽  
G.V.N Kiranmayi ◽  
J. M. Sruthi ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Wound Healing Activity ◽  
Ethyl Acetate Extracts ◽  
Abutilon Indicum

The present study was aimed at comparing the in vivo wound healing and in vitro antibacterial activity of Abutilon indicum roots. Wound healing is a complex phenomenon which consists of three stages inflammatory, proliferative, and remodelling. Traditionally, A. indicum is used for wound healing. There is no specific scientific data available for the wound healing activity of Abutilon indicum root. The present study was designed to investigate the same. The wound healing activity of ethanolic and ethyl acetate extracts of A. indicum was evaluated in incision and burn wound models. The parameters studied include tensile strength, epithelialisation period and rate of wound contraction. A. indicum also possesses antimicrobial activity. Ethyl acetate and ethanolic extract of A. indicum was tested against both Gram positive, Gram negative and fungal organisms using agar well diffusion method. From the results, it was concluded that ethyl acetate extract of A. indicum root had greater wound activity and antimicrobial activity than the ethanolic extract.

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