scholarly journals Dentin permeability of the apical third in different groups of teeth

2010 ◽  
Vol 21 (3) ◽  
pp. 216-219 ◽  
Author(s):  
Rodrigo Gonçalves Ribeiro ◽  
Melissa Andreia Marchesan ◽  
Ricardo Gariba Silva ◽  
Manoel D. Sousa-Neto ◽  
Jesus Djalma Pécora

This ex vivo study evaluated dentin permeability of the root canal in the apical third of different human groups of teeth. Eighty teeth were used, 8 from each dental group: maxillary and mandibular central incisors, lateral incisors and canines, maxillary first premolars (buccal and palatal roots), mandibular first premolars, and maxillary and mandibular second premolars, totalizing 88 roots that were distributed in 11 groups. The root canals were instrumented, irrigated with 1% NaOCl and 15% EDTA. Roots were immersed in 10% copper sulfate for 30 min and then in 1% rubeanic acid alcohol solution for the same period; this chemical reaction reveals dentin permeability by the formation of copper rubeanate, which is a dark-colored compound. Semi-serial 100-µm-thick cross-sections were obtained from the apical third of the roots. Five sections of each apical third were washed, dehydrated, cleared and mounted on glass slides for examination under optical microscopy. The percentage of copper ion infiltration and the amount of tubular dentin were quantified by morphometric analysis. The penetration of copper ions in the apical third ranged from 4.60 to 16.66%. The mandibular central and lateral incisors presented the highest dentin permeability (16.66%), while the maxillary canines and mandibular second and first premolars presented the lowest dentin permeability (4.60%, 4.80% and 5.71%, respectively; p<0.001). The other teeth presented intermediate permeability. In conclusion, dye penetration into dentin tubules at the apical region is strongly dependent on the group of teeth evaluated.

2016 ◽  
Vol 50 (9) ◽  
pp. 910-918 ◽  
Author(s):  
İ. Kaşıkçı Bilgi ◽  
İ. Köseler ◽  
P. Güneri ◽  
M. Hülsmann ◽  
M. K. Çalışkan

2014 ◽  
Vol 508 ◽  
pp. 012026
Author(s):  
E Pedullà ◽  
C Genovese ◽  
C Scolaro ◽  
M Cutroneo ◽  
G Tempera ◽  
...  
Keyword(s):  
Ex Vivo ◽  

Author(s):  
Luciana Viti Betti ◽  
Clovis Monteiro Bramante ◽  
Ivaldo Gomes de Moraes ◽  
Norberti Bernardineli ◽  
Roberto Brandão Garcia

BDJ Open ◽  
2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Maayan Shacham ◽  
Avi Levin ◽  
Avi Shemesh ◽  
Alex Lvovsky ◽  
Joe Ben Itzhak ◽  
...  

AbstractThe aim of the current study was to determine the accuracy of electronic apex locator (EAL) measurements when using files of different sizes in roots with wide apical foramina while considering a new parameter of stability of EAL reading. Ten teeth with straight roots were subjected to a sequential widening of the apical foramen to 0.6, 0.7, and 0.8 mm. The roots were embedded after each enlargement stage in an alginate mold and subjected to EAL readings. Measurements were done using sequential K-file sizes and the self-adjusting file (SAF). Measurement stability was introduced as a new additional parameter. As the difference between the file size used and the apical diameter of the canal decreases, the results obtained were more accurate and stable. The stability and accuracy of the measurements coincided with each other in a statistically significant manner. Within the limitations of the present ex vivo study, it may be concluded that in straight canals with wide apical foramina of 0.6–0.8 mm, both SS K-files which fit snugly to the walls of apical foramen and the SAF file may offer both accurate and stable EAL measurements.


Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Fauzia Ashraf ◽  
Pushpa Shankarappa ◽  
Abhinav Misra ◽  
Asheesh Sawhney ◽  
Nandamuri Sridevi ◽  
...  

Introduction. The aim of the present study was to evaluate the dentinal cracks after root canal preparation with rotary files: Gates Glidden, ProTaper Universal, ProTaper Next, and HyFlex CM at different instrumentation lengths.Methodology. Sixty-five mandibular premolars were mounted in the acrylic tube with simulated periodontal ligaments and the apex was exposed. The root canals were instrumented with different rotary files, namely, ProTaper Universal, ProTaper Next, and HyFlex CM, to the major apical foramen (AF), short AF, and beyond AF. The root apex was stained with 1% methylene blue dye and digital images of apical surface of every tooth were taken and development of dentinal defects was determined by using stereomicroscope. Multinomial logistic regression test was performed to identify influencing factors.Results. Instrumentation with rotary files terminated 2 mm short AF and did not cause any cracks. Significantly less cracks were seen when instrumentation with rotary files terminated 1 mm short apical foramen when compared with the instrumentation terminated at or beyond apical foramen (p<0.05).Conclusion. ProTaper Universal rotary files caused more dentinal cracks than ProTaper Next and HyFlex CM. Instrumentation short AF reduced the risk of dentinal defects.


Author(s):  
Giovanna Dornelas Mantovani ◽  
Brenda Paula Figueiredo de Almeida Gomes ◽  
Lidiane Mendes Louzada ◽  
Rodrigo Arruda Vasconcelos ◽  
Maria Cristina Carvalho ◽  
...  

The present study evaluated the effectiveness of XP-Endo Finisher (XPF) associated with XP-Endo Shaper (XPS) or Reciproc Blue (RB) files in the reduction of Enterococcus faecalis in oval-shaped root canals. Eighty mandibular incisors with single oval-shaped root canals were contaminated with E. faecalis for 10 days at 37°C with centrifugation on alternate days. The teeth were randomly assigned to eight experimental groups (n=10) as follows: G1 – XP-Endo Shaper, G2 – XP-Endo Shaper + XP-Endo Finisher, G3 – Reciproc Blue, and G4 – Reciproc Blue + XP-Endo Finisher. Chemomechanical preparation was performed with saline solution (NaCl) or 2.5% sodium hypochlorite (NaOCl). Microbial reduction was assessed by colony forming units (CFU/mL) count before (S1) and after (S2) CMP by using sterile paper points. ANOVA, Tukey’s test and Bonferroni’s post-hoc tests were used at 5% significance level. Bacteria were present in all initial samples (P>.05). All instrumentation techniques reduced bacteria, irrespective of the irrigating solution (P<.05). With the use of NaCl, Reciproc Blue was more effective than XP-Endo Shaper (P=.035). The association of XP-Endo Finisher improved the cleaning efficacy of both systems (P=.239). With the use of NaOCl, XP-Endo Shaper and Reciproc Blue presented similar effectiveness (P=.779). XP-Endo Finisher enhanced the bacterial reduction of both systems tested (P<.05). The use of NaOCl improved the CMP, irrespective of the instrumentation technique used (P<.05).


2012 ◽  
Vol 13 (1) ◽  
pp. 23-26 ◽  
Author(s):  
KG Nischith ◽  
GPV Srikumar ◽  
Shuaib Razvi ◽  
R Vinay Chandra

ABSTRACT Aim The purpose of this study was to evaluate the effect of smear layer on apical seal of endodontically treated teeth. Materials and methods Sixty freshly extracted human maxillary central incisor teeth were selected and were randomly divided into two experimental groups. Group A of 25 teeth and group B of 25 teeth and a control group of 10 teeth. Cleaning and shaping of the root canals were performed using endodontic K-files up to no. 50 size file in step-back technique. During the process, in root canals of group A, 17% EDTA (ethylenediaminetetraacetic acid) followed by 3% NaOCl (sodium hypochlorite) was used as root canal irrigant. In group B, MTAD (mixture tetracycline citric acid and detergent) was used as a root canal irrigant. In control group, saline was used as root canal irrigant. The root surfaces were then coated with nail polish of both experimental groups and control group. The samples were then immersed in India ink dye for 1 week at 37°C. Results The statistical analysis of the results showed that the apical leakage was significantly more in teeth, where smear layer was not removed. Conclusion The removal of smear layer improves the longterm apical seal and success of endodontically treated teeth. Clinical significance The development and maintenance of apical seal is desirable and considered to be a major prerequisite to improve the outcome of root canal treatment. How to cite this article Nischith KG, Srikumar GPV, Razvi S, Chandra RV. Effect of Smear Layer on the Apical Seal of Endodontically Treated Teeth: An ex vivo Study. J Contemp Dent Pract 2012;13(1):23-26.


2019 ◽  
Vol 45 (2) ◽  
pp. 129-135 ◽  
Author(s):  
Thomas Gerhard Wolf ◽  
Stefan Wentaschek ◽  
Richard J. Wierichs ◽  
Benjamín Briseño-Marroquín

2017 ◽  
Vol 46 (3) ◽  
pp. 153-157
Author(s):  
Juliane Maria GUERREIRO-TANOMARU ◽  
Alana Priscila SOUZA AGUIAR ◽  
Gisselle Moraima CHÁVEZ-ANDRADE ◽  
Maria Inês Basso BERNARDI ◽  
Mário TANOMARU-FILHO

Abstract Introduction Nanoparticles and associations to calcium hydroxide can be used to increase antimicrobial activity. Objective To evaluate antibacterial activity against Enterococcus faecalis and pH of intracanal medications (IM) based on calcium hydroxide (CH) and zinc oxide (ZnO) micro- or nanoparticles, and their association with 0.4% chlorhexidine (CHX). Material and method Root canals from single-rooted human teeth were inoculated and incubated for 21 days. After sample (S1), the root canals were filled with the medications for 7 days and samples were collected immediately after medication (S2) and 7 days later (S3). Counting of CFU mL-1 was performed. Polyethylene tubes filled with the medications were used for the pH evaluation after 3, 7, 14 and 28 days. Data were submitted to ANOVA and Tukey tests (p<0.05). Result All IM promoted elimination of E. faecalis immediately after medication (S2). All the pastes promoted a similar pH increase. Conclusion CH/ZnO micro- or nanoparticles associated with CHX promoted greater bacterial reduction in the root canals and similar pH.


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