scholarly journals RAPD analysis revealing polymorphism in egg parasitoids of soybean stink bugs (Hemiptera: Pentatomidae)

1998 ◽  
Vol 27 (3) ◽  
pp. 413-420 ◽  
Author(s):  
Salah M. Aljanabi ◽  
Marta S. Loiácono ◽  
Rodrigo T. Lourenço ◽  
Miguel Borges ◽  
Myrian S. Tigano
Keyword(s):  
Rapd Markers ◽  
Egg Parasitoids ◽  
Correct Identification ◽  
Stink Bugs ◽  
Soybean Field ◽  
Random Amplification ◽  
Identification Of Species ◽  
Glycine Max L ◽  
Polymerase Chain ◽  
Telenomus Podisi

The solitary egg parasitoids (Hymenoptera: Scelionidae) of Pentatomidae species complex are potential candidates for soybean (Glycine max L.) integrated pest management programs in Brazil. The correct identification of species and strains of these parasitoids is necessary to implement the use of biological control. Polymerase chain reaction (PCR) was used to generate random amplification of polymorphic DNA (RAPD) from male individuals of Telenomus podisi Ashmead and Trissolcus spp. collected in a soybean field in Brasília, Brazil in 1996. The analysis of 18 primers used to screen Trissolcus spp. individuals revealed different patterns related to the species analyzed. These results indicate the potential of RAPD markers to differentiate T. teretis Johnson, T. urichi Crawford and T. basalis (Wollaston). The 13 selected primers used to analyze T. podisi individuals produced 103 clear polymorphic scorable markers. Different genotypes were observed within this population analyzed, although the cluster analysis applied to the RAPD data showed high homogeneity (similarity >78.2%) among individuals.

scholarly journals Genetic profiling of mistletoe (Viscum album L.) using RAPD-analysis

2020 ◽  
Vol 26 ◽  
pp. 82-86
Author(s):  
Yu. O. Bilonozhko ◽  
A. N. Rabokon ◽  
A. S. PostovoitovA ◽  
L. O. Kalafat ◽  
S. M. PrivAlikhin ◽  
...  
Keyword(s):  
Rapd Markers ◽  
Rapd Analysis ◽  
Molecular Genetic ◽  
Viscum Album ◽  
Dna Fragments ◽  
Coniferous Species ◽  
Genetic Profiling ◽  
Random Amplification ◽  
Polymerase Chain ◽  
Genetic Profiles

Aim. The aim of this research was genetic profiling and identification of genetic differences between V. album speciments, growing on deciduous and coniferous species of woody plants using RAPD markers. Methods. The method of polymerase chain reaction (PCR) with random primers (Random Amplification of Polymorphic DNA - RAPD) was used. Amplified DNA fragments were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using the staining with silver nitrate. Results. All the studied mistletoe samples were differentiated from each other, and their unique molecular genetic profiles were obtained. 241 amplified DNA fragments were detected in the range from 200 to 2000 bp, 152 fragments (63%) were polymorphic. The samples were divided into two separate groups depending on the type of host plant. Conclusions. The fact that the samples formed two separate clades confirms the assumption that mistletoe, which grow on pine and grow on maple, represents two separate subspecies of V. album. Keywords: Viscum album L., molecular genetic markers, polymorphism, RAPD.

Genetic diversity among microsporidian isolates from the silkworm, Bombyx mori, as revealed by randomly amplified polymorphic DNA (RAPD) markers

2011 ◽  
Vol 56 (4) ◽  
Author(s):  
B. Surendra Nath ◽  
W. Hassan ◽  
S. Nageswara Rao ◽  
N. Vijaya Prakash ◽  
S. Gupta ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Type Species ◽  
Rapd Markers ◽  
Randomly Amplified Polymorphic Dna ◽  
Chain Reaction ◽  
Rapd Pcr ◽  
Random Amplification ◽  
Major Cluster ◽  
Sources Of Infection ◽  
Polymerase Chain

AbstractRandom amplification of polymorphic DNA polymerase chain reaction (RAPD-PCR) was carried out to assess the genetic diversity of five new microsporidian isolates viz., NIWB-11bp, NIWB-12n, NIWB-13md, NIWB-14b and NIWB-15mb identified from the silkworms. A type species, NIK-1s_mys was used as control for comparison. Differences in the spore shape, length and width were observed. Of the 30 decamer random primers tested, 22 primers gave repeatable RAPD profiles and yielded a total of 143 fragments, of which 78 were polymorphic (55%). The resulting data was used to derive genetic similarity values for constructing a dendrogram. The neighbour joining method based on Dice coefficients indicate a major cluster comprising NIK-1s_mys, NIWB-11bp and NIWB-12n, whereas NIWB-13md, NIWB-14b and NIWB-15mb appear to be different from each other as well from the major cluster mentioned above which includes the type species (NIK-1s_mys). Based on the reproducibility of RAPD profiles, we are able to identify these microsporidians as different isolates. The RAPD technique may be useful in detecting sources of infection of this economically important domestic insect.

scholarly journals New Records of Egg Parasitoids of Stink Bugs (Hemiptera: Pentatomidae) on Rice in Piauí, Brazil: Rate Parasitism, Incidence and Seasonality

2021 ◽  
Vol 3 ◽  
pp. ec03020
Author(s):  
Nadja N. P. Silva ◽  
Kátia K. A. Sousa ◽  
Paulo Henrique S. Silva ◽  
Ranyse B. Querino
Keyword(s):  
Rainy Season ◽  
New Records ◽  
Egg Parasitoids ◽  
Stink Bugs ◽  
Parasitoid Species ◽  
Naturally Occurring ◽  
Euschistus Heros ◽  
Dry Seasons ◽  
Telenomus Podisi

This study was conducted to investigate parasitism of naturally occurring pentatomid eggs in rice crops in a Cerrado-Caatinga ecosystem during the rainy and dry seasons in Teresina, Piauí, Brazil. Also, it provides information on the incidence and seasonality of parasitoid species. Parasitism was registered only during the rainy season, where Telenomus podisi (Ashmead, 1893) parasitized Oebalus poecilus (Dallas, 1851), Tibraca limbativentris (Stal, 1860) and Euschistus heros (Fabricius, 1798) eggs. Additionally, Trissolcus urichi (Crawford, 1913) was collected from O. poecilus and T. limbativentris eggs. Ooencyrtus anasae (Ashmead, 1887) was found in O. poecilus eggs. Such findings provide information that can be used to supplement the management of pentatomid pests in the rice agroecosystems, in Piauí, Brazil.

Response of the Egg Parasitoids Trissolcus basalis and Telenomus podisi to Compounds from Defensive Secretions of Stink Bugs

2009 ◽  
Vol 35 (1) ◽  
pp. 8-19 ◽  
Author(s):  
Raúl A. Laumann ◽  
Michely F. S. Aquino ◽  
Maria C. B. Moraes ◽  
Martín Pareja ◽  
Miguel Borges
Keyword(s):  
Egg Parasitoids ◽  
Stink Bugs ◽  
Defensive Secretions ◽  
Trissolcus Basalis ◽  
Telenomus Podisi

scholarly journals Random Amplification of Polymorphic DNA Analysis for Genetic Characterization of Two Breeds of Dogs, German Shepherd and Japanese Spitz

2013 ◽  
Vol 13 (2) ◽  
pp. 73-78
Author(s):  
Jarina Joshsi ◽  
Lumanti Manandhar ◽  
Patima Shrestha ◽  
Rani Gupta ◽  
Rojlina Manadhar ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Genetic Characterization ◽  
Random Amplified Polymorphic Dna ◽  
Neighbor Joining ◽  
German Shepherd ◽  
Polymorphic Loci ◽  
Random Amplification

Random amplified polymorphic DNA (RAPD) markers were used to study genetic diversity in dog samples belonging to populations of German Shepherd and Japanese Spitz. A total of twelve samples were typed using eight RAPD primers. Out of eight primers, three primers gave result in six individuals of dogs. The phylogenetic tree constructed by the neighbor joining method based on Nei. Original measures revealed highest genetic identity found in German Shepherd as 0.9444 and highest genetic distance as 1.2809. The analysis predicts the number of polymorphic loci as 15 and the percentage of polymorphic loci as 83.3. Nepal Journal of Science and Technology Vol. 13, No. 2 (2012) 73-78 DOI: http://dx.doi.org/10.3126/njst.v13i2.7717

scholarly journals Environmental diagnosing of the new algal pollution of Tigris River in Iraq

2021 ◽  
Vol 877 (1) ◽  
pp. 012024
Author(s):  
Warqaa Y. Salih ◽  
Fikrat M. Hassan
Keyword(s):  
Ribosomal Rna ◽  
Computer Software ◽  
Analysis Data ◽  
Molecular Data ◽  
Algal Flora ◽  
Tigris River ◽  
Identification Of Species ◽  
Phylogenetic Identification ◽  
Polymerase Chain ◽  
Flora Of Iraq

Abstract The purpose of this study is to use eDNA in the biodiversity of the Tigris river’s sediment. Algal samples were collected and examined under light microscopy. The collected algae were cultured, and after their growth, the DNA extractions were made from culture and amplified 16S ribosomal RNA gene partial sequences data by Polymerase Chain Reaction (PCR). Phylogenetic identification of species was conducted by the evaluation of obtained sequence analysis data by using computer software. Leptolyngbya benthonica (MN 714226.1) and Nostoc paludosum (MN 714225.1) were identified by molecular analysis and registered at NCBI and considered as a new record to the algal flora of Iraq. Implementing molecular data in the taxonomy of species will be essential to solve the taxonomic problems associated with microscopic methods.

Inheritance of random amplified polymorphic DNA markers in an interspecific cross in the genus Stylosanthes

Genome ◽  
1993 ◽  
Vol 36 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Kemal Kazan ◽  
John M. Manners ◽  
Don F. Cameron
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Rapd Markers ◽  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Interspecific Cross ◽  
Parental Origin ◽  
Chain Reaction ◽  
Stylosanthes Hamata ◽  
Polymerase Chain

The inheritance of random amplified polymorphic DNA (RAPD) markers generated via the polymerase chain reaction amplification of genomic DNA sequences in an F2 family of an interspecific cross between Stylosanthes hamata and S. scabra was investigated. An initial comparison between the parental species, S. hamata cv. Verano and S. scabra cv. Fitzroy, demonstrated that 34% of detected RAPD bands were polymorphic. Of 90 primers tested, 35 showed relatively simple and reliably scorable polymorphisms and were used for segregation analysis. Sixty F2 individuals were scored for the segregation of 73 RAPD markers and 55 of these markers fit a 3:1 ratio. Segregation of eight other RAPD markers deviated significantly from a 3:1 ratio. There was no bias in the inheritance of RAPD markers regarding parental origin of the segregating RAPD markers. Linkage analysis revealed 10 linkage groups containing a total of 44 RAPD loci. Another 10 RAPD markers (7 of maternal origin) that were polymorphic between the parents did not segregate in the F2 population. One of the maternally inherited RAPD bands hybridized to chloroplast DNA. Analysis of RAPD loci by DNA hybridization indicated that mainly repeated sequences were amplified. These data indicate that RAPDs are useful genetic markers in Stylosanthes spp. and they may be suitable for genetic mapping.Key words: genetic mapping, molecular markers, polymerase chain reaction, Stylosanthes hamata, Stylosanthes scabra.

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