scholarly journals Environmental diagnosing of the new algal pollution of Tigris River in Iraq

2021 ◽  
Vol 877 (1) ◽  
pp. 012024
Author(s):  
Warqaa Y. Salih ◽  
Fikrat M. Hassan
Keyword(s):  
Ribosomal Rna ◽  
Computer Software ◽  
Analysis Data ◽  
Molecular Data ◽  
Algal Flora ◽  
Tigris River ◽  
Identification Of Species ◽  
Phylogenetic Identification ◽  
Polymerase Chain ◽  
Flora Of Iraq

Abstract The purpose of this study is to use eDNA in the biodiversity of the Tigris river’s sediment. Algal samples were collected and examined under light microscopy. The collected algae were cultured, and after their growth, the DNA extractions were made from culture and amplified 16S ribosomal RNA gene partial sequences data by Polymerase Chain Reaction (PCR). Phylogenetic identification of species was conducted by the evaluation of obtained sequence analysis data by using computer software. Leptolyngbya benthonica (MN 714226.1) and Nostoc paludosum (MN 714225.1) were identified by molecular analysis and registered at NCBI and considered as a new record to the algal flora of Iraq. Implementing molecular data in the taxonomy of species will be essential to solve the taxonomic problems associated with microscopic methods.

scholarly journals Isolation and Molecular Identification of Some Blue-Green Algae (Cyanobacteria) from Freshwater Sites in Tokat Province of Turkey

2017 ◽  
Vol 5 (11) ◽  
pp. 1371 ◽  
Author(s):  
Tunay Karan ◽  
Ramazan Erenler ◽  
Zekeriya Altuner
Keyword(s):  
Sequence Analysis ◽  
Green Algae ◽  
Computer Software ◽  
Analysis Data ◽  
Molecular Data ◽  
Isolated Cells ◽  
Laboratory Environment ◽  
Blue Green Algae ◽  
Identification Of Species ◽  
Phylogenetic Identification

Collected blue-green algae (cyanobacteria) from freshwater sites throughout Tokat province and its outlying areas were isolated in laboratory environment and their morphological systematics were determined and also their species identifications were studied by molecular methods. Seven different species of blue-green algae collected from seven different sites were isolated by purifying in cultures in laboratory environment. DNA extractions were made from isolated cells and extracted DNAs were amplified by using PCR. Cyanobacteria specific primers were used to amplify 16S rRNA and phycocyanine gene regions using PCR. Phylogenetic identification of species were conducted by evaluation of obtained sequence analysis data by using computer software. According to species identification by sequence analysis, it was seen that molecular data supports morphological systematics.

scholarly journals Molecular Identification of Trypanosoma evansi Isolated from Arabian Camels (Camelus dromedarius) in Riyadh and Al-Qassim, Saudi Arabia

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1149
Author(s):  
Dina M. Metwally ◽  
Isra M. Al-Turaiki ◽  
Najwa Altwaijry ◽  
Samia Q. Alghamdi ◽  
Abdullah D. Alanazi
Keyword(s):  
Saudi Arabia ◽  
Infection Rate ◽  
Ribosomal Rna ◽  
Phylogenetic Analyses ◽  
Trypanosoma Evansi ◽  
Camelus Dromedarius ◽  
18S Ribosomal Rna ◽  
18S Ribosomal Rna Gene ◽  
Polymerase Chain ◽  
Pcr Targeting

We analyzed the blood from 400 one-humped camels, Camelus dromedarius (C. dromedarius), in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite Trypanosoma spp. Polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) gene was used to detect the prevalence of Trypanosoma spp. in the camels. Trypanosoma evansi (T. evansi) was detected in 79 of 200 camels in Riyadh, an infection rate of 39.5%, and in 92 of 200 camels in Al-Qassim, an infection rate of 46%. Sequence and phylogenetic analyses revealed that the isolated T. evansi was closely related to the T. evansi that was detected in C. dromedarius in Egypt and the T. evansi strain B15.1 18S ribosomal RNA gene identified from buffalo in Thailand. A BLAST search revealed that the sequences are also similar to those of T. evansi from beef cattle in Thailand and to T. brucei B8/18 18S ribosomal RNA from pigs in Nigeria.

scholarly journals Clinical Evaluation of a 16S Ribosomal RNA Polymerase Chain Reaction Test for the Diagnosis of Lymph Node Tuberculosis

2006 ◽  
Vol 43 (7) ◽  
pp. 855-859 ◽  
Author(s):  
Fernando Osores ◽  
Oscar Nolasco ◽  
Kristien Verdonck ◽  
Jorge Arevalo ◽  
Juan Carlos Ferrufino ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Node ◽  
Rna Polymerase ◽  
Ribosomal Rna ◽  
Polymerase Chain Reaction Test ◽  
Chain Reaction ◽  
Reaction Test ◽  
Polymerase Chain ◽  
Lymph Node Tuberculosis ◽  
Chain Reaction Test

scholarly journals Detection of Periprosthetic Infections With Use of Ribosomal RNA-Based Polymerase Chain Reaction

2010 ◽  
Vol 92 (3) ◽  
pp. 654-663 ◽  
Author(s):  
Patrick F Bergin ◽  
Jason D Doppelt ◽  
William G Hamilton ◽  
Gudrun E Mirick ◽  
Angela E Jones ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Ribosomal Rna ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Periprosthetic Infections

DIVERSITY OF GRACILARIACEAE (RHODOPHYTA) IN BRAZIL: INTEGRATING MORPHOLOGICAL AND MOLECULAR DATA

Phytotaxa ◽  
2021 ◽  
Vol 496 (1) ◽  
pp. 1-53
Author(s):  
GOIA DE MATTOS LYRA ◽  
JOSÉ MARCOS DE CASTRO NUNES ◽  
EDILENE MARIA DOS SANTOS PESTANA ◽  
JOÃO CARLOS GAMA DE MATOS ◽  
TAIARA AGUIAR CAIRES ◽  
...  
Keyword(s):  
Economic Value ◽  
Molecular Data ◽  
Tropical Species ◽  
Brazilian Coast ◽  
Type Specimens ◽  
Mitochondrial Coi ◽  
Dichotomous Key ◽  
Identification Of Species ◽  
Morphological And Molecular Data ◽  
Selection Of Species

The Gracilariaceae comprises 242 mainly tropical species, including some with significant economic value for the food and pharmaceutical industry. Accurate taxonomy is crucial for the selection of species for cultivation. However, species identification is still challenging taxonomists. Nineteen species of Gracilariaceae have so far been referred to the Brazilian coast. We describe this diversity across approximately 32 degrees of latitude, using mitochondrial (COI-5P) and plastid (UPA and rbcL) markers in species delimitation analyses (ABGD and SPN). We provide phylogenetic (based on a concatenated three-genes tree and a taxa rich rbcL tree) and barcoding analyses from a broad and partially original collection combined with morphoanatomical data, including information resulting from analyses of type specimens and topotype material. We identified twenty-five specific entities occurring on the Brazilian coast, including four new species yet to be described. Spermatangial arrangements and aspects of thallus morphology were the most useful characters for species and genera delimitation, while characters based on the anatomy of the cystocarp overlap. Our morphological analyses demonstrate the lack of exclusive characters that justify the recently proposed Gracilariaceae infrafamilial categories, and therefore we use in this work the Gracilaria sensu lato genus concept. We provide descriptions, illustrations and a dichotomous key for the identification of species occurring in Brazil.

scholarly journals Viability of pathogenic dermatophytes during a 4-week treatment with 1% topical luliconazole for tinea pedis

2019 ◽  
Vol 58 (3) ◽  
pp. 401-403 ◽  
Author(s):  
Tomoyuki Iwanaga ◽  
Tsuyoshi Ushigami ◽  
Kazushi Anzawa ◽  
Takashi Mochizuki
Keyword(s):  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Copy Number ◽  
Tinea Pedis ◽  
Pathogenic Fungi ◽  
Topical Administration ◽  
Initial Visit ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Average Copy

Abstract The viability of pathogenic fungi in the scale was investigated during topical administration of 1% luliconazole (LLCZ). Thirteen tinea pedis patients found to be positive on KOH examination were assessed by mycological examinations and quantitative real-time polymerase chain reaction (PCR) targeted internal transcribed spacer (ITS) in ribosomal RNA gene at the initial visit and after 2 and 4 weeks of treatment. Assays showed that the average copy number of ITS DNA had significantly decreased to 22.9% at 2 weeks and 4.8% at 4 weeks compared with the initial visit. LLCZ topical treatment could defeat almost pathogenic dermatophytes in the scales within 4 weeks.

Molecular data provide new insights into the phylogeny of Cladonotinae (Orthoptera: Tetrigoidea) from China with the description of a new genus and species

Zootaxa ◽  
2020 ◽  
Vol 4809 (3) ◽  
pp. 547-559
Author(s):  
RONG-JIAO ZHANG ◽  
CONG-LIN ZHAO ◽  
FEI-PENG WU ◽  
WEI-AN DENG
Keyword(s):  
Ribosomal Rna ◽  
Phylogenetic Relationships ◽  
New Genus ◽  
Molecular Data ◽  
18S Rrna Gene ◽  
Rrna Gene ◽  
Mitochondrial Cytochrome Oxidase Subunit ◽  
Close Relationship ◽  
Phylogenetic Framework ◽  
Mitochondrial Cytochrome Oxidase

Considerable effort has been devoted to elucidating the phylogenetic relationships of tetrigides. However, there is still no commonly accepted phylogenetic hypothesis. Therefore, the phylogenetic relationships among some subfamilies remain unclear; e.g., Cladonotinae is a controversial group, in which the phylogenetic relationships between genera and the boundaries of some of the included genera are unclear, causing some of the taxa to be difficult to identify. Therefore, an in-depth phylogenetic analysis of Cladonotinae is urgently needed. In this study, a robust phylogenetic framework for the tetrigides was reconstructed based on the combined mitochondrial cytochrome oxidase subunit I (COI), 16S ribosomal RNA (16S rRNA), and nuclear 18S ribosomal RNA (18S rRNA) gene sequences of 25 species belonging to 16 genera of Tetrigoidea from China, which included 13 species from 8 genera of Cladonotinae. Phylogenetic inferences were performed using the combined dataset and Bayesian inference (BI) and Maximum Parsimony (MP) methods, and the phylogenetic tree of Cladonotinae was reconstructed. All inferences based on the results of the present study supported the Cladonotinae subfamily as a polyphyletic group; within the Cladonotinae subfamily, Tetradinodula, and Tuberfemurus were closely related to Tetriginae, while Austrohancockia and Gibbotettix showed a close relationship to the Scelimenidae subfamily. Additionally, a new genus and new species of the Cladonotinae subfamily are described and illustrated: Hainantettix Deng, gen. nov. and Hainantettix strictivertex Deng, sp. nov. 

A new species of Omolicna (Hemiptera: Auchenorrhyncha: Fulgoroidea: Derbidae) from coconut palm in Costa Rica and new country records for Omolicna brunnea and Omolicna triata

Zootaxa ◽  
2019 ◽  
Vol 4577 (3) ◽  
pp. 501 ◽  
Author(s):  
BRIAN W. BAHDER ◽  
CHARLES R. BARTLETT ◽  
EDWIN A. BARRANTES BARRANTES ◽  
MARCO A. ZUMBADO ECHAVARRIA ◽  
ALESSANDRA R. HUMPHRIES ◽  
...  
Keyword(s):  
New Species ◽  
Costa Rica ◽  
Ribosomal Rna ◽  
Sequence Data ◽  
Cocos Nucifera ◽  
Molecular Data ◽  
New Taxon ◽  
Additional Species ◽  
New Subgenus ◽  
A New Species

An ongoing survey for novel phytoplasmas and viruses that affect palms (Arecaceae) is being conducted in Costa Rica along with potential vectors. During that survey, a new species of derbid planthopper (Hemiptera: Fulgoroidea) in the genus Omolicna Fennah was detected from Heredia and Limón State and is here described as Omolicna xavieri sp. n. This new taxon was first found on declining coconut palms (Cocos nucifera) and subsequently on healthy coconut palms in Tortuguero National Park. Also, two additional species of Omolicna, O. brunnea and O. triata, were collected on coconut in the same habitat and represent new country records. There are no previously published records of Omolicna from Costa Rica. Sequence data for the cytochrome c oxidase subunit I (COI) and the 18S ribosomal RNA gene were obtained. In addition, novel 18S primers specific to auchenorrhynchan insects were developed and presented. The molecular data show high pairwise distances between O. xavieri sp. n. and other Omolicna species (24% to 31% and 10% using COI and 18S, respectively). Based on both morphological and sequence data, we erect and briefly describe the new subgenus Agoo for the new species to reflect the observed differences. A checklist of Omolicna species is presented. 

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