scholarly journals Inibição do desenvolvimento fúngico através da utilização de óleos essenciais de condimentos

2006 ◽  
Vol 30 (4) ◽  
pp. 731-738 ◽  
Author(s):  
Marcelo Cláudio Pereira ◽  
Georgia Rocha Vilela ◽  
Lívia Martinez Abreu Soares Costa ◽  
Reginaldo Ferreira da Silva ◽  
Anderson Felicori Fernandes ◽  
...  

Objetivou-se com este trabalho avaliar os efeitos inibitórios, "in vitro", de óleos essenciais dos condimentos, alecrim (Rosmarinus officinalis L.), cebola (Allium cepa L.), manjericão (Ocimum basilicum L.), menta (Mentha piperita L.) e orégano (Origanum vulgare L.), sobre o desenvolvimento de fungos. Os óleos foram extraídos pela técnica de arraste a vapor e testados nas concentrações de 500; 1000; 1500 e 2000 mg/mL-1. Como culturas de teste foram utilizados os fungos Fusarium sp.; Aspergillus ochraceus Wilhelm.; Aspergillus flavus Link e Aspergillus niger van Tieghem obtidos da micoteca do EcoCentro/EPAMIG em Lavras, MG. O óleo essencial do orégano inibiu o desenvolvimento dos fungos testados em todas as concentrações exceto o fungo A. niger que teve o seu desenvolvimento micelial inibido a partir da concentração de 1000 mg/mL-1,. Os óleos de alecrim, menta, cebola e manjericão tiveram um efeito pronunciado a partir da concentração de 1500 mg/mL-1.

2017 ◽  
Vol 24 (0) ◽  
Author(s):  
Vinícius Spolaor Fantinel ◽  
Luciana Magda de Oliveira ◽  
Ricardo Trezzi Casa ◽  
Priscilla Félix Schneider ◽  
Emerson Couto da Rocha ◽  
...  

RESUMO Acca sellowiana é uma espécie frutífera nativa da Região Sul do Brasil que está despertando grande interesse econômico devido ao alto potencial organoléptico de seus frutos. A principal forma de propagação da espécie é via sexuada. Objetivando determinar a qualidade sanitária das sementes, foram comparadas três formas de detecção de fungos, com ou sem a assepsia superficial, em sementes oriundas de quatro municípios do Estado do Rio Grande do Sul e de três municípios do Estado de Santa Catarina. Foram testados os meios de cultura Batata-Dextrose-Ágar (BDA), V8 (suco de tomate) e o método Blotter Test. Foram identificados os seguintes fungos: Aspergillus niger, Aspergillus flavus, Penicillium sp., Colletotrichum gloeosporioides, Fusarium sp., Curvularia sp., Alternaria sp., Trichoderma sp., Epicoccum sp. e Phomopsis sp. Os meios agarizados são mais sensíveis na detecção de fungos em sementes de goiaba serrana. A assepsia das sementes reduz a incidência de fungos infestantes.


Author(s):  
Christian Andrei Chacín Zambrano

Colombia por su privilegiada ubicación geográfica y gran variedad de biomas y ecosistemas, ofrece una rica diversidad en flora estimada en más de 45.000 especies, equivalente al 10% del total de los géneros que existen a nivel mundial1. Además, nuestra biodiversidad nos lleva a ostentar el primer lugar en variedades de orquídeas, con 4.010 especies, y a su vez el tercero en variedades de palma, con 231 especies2.  La distribución de la riqueza vegetal en nuestro país muestra la región andina como la más favorecida, seguida por el chocó y la amazonia3. Dentro de la región andina, Santander es uno de los departamentos con mayor número de ecosistemas, gracias a su geomorfología, siendo potencia en la actividad agroforestal la cual ha sido vital en el desarrollo de Santander, donde indudablemente su fortaleza se encuentra en los cultivos permanentes, con un total sembrado de 171. 845 hectáreas de los cuales la caña, la mora y la piña aportan un 16% total4. Sin embargo, la ejecución de políticas inadecuadas de ocupación y utilización del territorio, la deforestación, la sobreexplotación y demás métodos para la producción masiva ha ido deteriorando poco a poco los hábitats poniendo en peligro muchas de las especies nativas de la región por falta de conocimiento al llevar a cabo sistemas agroforestales sostenibles5. Es por ello que, siendo conscientes de las necesidades de la región por salvaguardar los recursos genéticos, se estableció un banco de germoplasma con el fin de preservar las especies propias y que por años han sido un motor en el sector productivo agropecuario para el Departamento de Santander como lo son la Piña oro miel (Ananas sativus (lindl) schult), la guayaba silvestre (Psidium guajava l.),  la mora de castilla (Rubus glaucus), la caña de azúcar (Saccharum officinarum), frijol arbustivo (Phaseolus vulgaris) (y aromáticas tales como Orégano (Origanum vulgare), Mentha Piperita (Mentha x piperita), utilizando la técnica del cultivo in vitro vegetal, para ello se estandarizó  un método de desinfección teniendo en cuenta el tipo de desinfectante, las concentraciones y tiempos de exposición con el explante, realizando mediciones de porcentaje de oxidación, porcentaje de explantes prósperos y porcentajes de explantes contaminados; así mismo, se desarrolló la fase de establecimiento de cada uno de los cultivares a partir de diferentes formulaciones en medios de cultivos teniendo en cuenta los tiempos de formación de brotes, el porcentaje de germinación entre otros, para finalizar con el establecimiento de la fase de multiplicación de los explantes, donde se evaluaron variables tales altura de la plántula, número de hojas, tamaño de la raíz y la relación de multiplicación. Dentro de los resultados generados se obtuvo protocolos para el proceso de desinfección, las formulaciones de los medios de cultivo para cada uno de las especies en su fase de establecimiento y multiplicación y a su vez, una relación de multiplicación de 1:20, es decir que a partir de un explante, se alcanzó la multiplicación de 20 plántulas.Cómo citar este resumen: Chacín Zambrano C A, La Biotecnología Vegetal Como Herramienta Para La Conservación De La Biodiversidad, BUCARAMANGA, COLOMBIA. Innovaciencia facultad cienc. exactas fis. naturales. 2017; 5(1).


2017 ◽  
Vol 29 (1) ◽  
pp. 07
Author(s):  
Gulshan Irshad ◽  
Hira Gazal ◽  
Farah Naz ◽  
Imran Hassan ◽  
Amir Bashir ◽  
...  

The present study was conducted to detect seed borne mycoflora associated with seven cultivars of sunflower (Helianthus annuus L.) and five cultivars of Zinnia (Zinnia elegans L.) by using Agar and Blotter paper methods. A total 12 genera of fungi were detected including; Alternaria alternata, Aspergillus flavus, Aspergillus niger, Cladosporium sp., Stemphylium helianthi, Penicillium sp., Fusarium oxysporum, Mucor sp., Fusarium moniliforme, Fusarium solani, Rhizocotonia solani and Rhizopus sp. The detected fungi depleted germination  10-30%, 10-20% and seedling mortality 10-17%, 10-12%. in sunflower and Zinnia respectively. A comparative study was designed to evaluate three  fungicides to control  seed mycoflora at the rate of of 1.5g/kg, 2.0g/kg, 2.5g/kg, 3.0g/kg, Maximum germination percentage was attained in case of seed dressing with Topsin M-70  followed by Bayton and Dithane M-45 at the rate of 3.0g/kg. 


2007 ◽  
Vol 74 (1) ◽  
pp. 49-53
Author(s):  
M.O. Xavier ◽  
I.M. Madrid ◽  
A.R.M. Meinerz ◽  
M.B. Cleff ◽  
L.F.D. Schuch ◽  
...  

RESUMO A aspergilose é causada por fungos ubíquos e oportunistas do gênero Aspergillus, que liberam milhares de conídios no ar, contaminando o ambiente, sendo de extrema importância a utilização de filtros de ar e programas corretos de anti-sepsia e desinfecção para prevenção da enfermidade. Este estudo avaliou a eficácia “in vitro” dos agentes químicos, iodóforo, amônia quaternária e clorexidina, frente a isolados de Aspergillus fumigatus (8), Aspergillus niger (8), Aspergillus flavus (6) e Aspergillus terreus (1). Para o teste foram preparadas diluições sucessivas dos desinfetantes/antisépticos (log2) em caldo RPMI, e os inóculos foram ajustados até uma concentração final de 5 x 104 UFC/mL. Foi realizada a técnica de microdiluição em caldo de acordo com NCCLS M-38, adaptada para agentes químicos, com incubação das microplacas a 35° C em agitação constante. A leitura visual dos resultados foi realizada após 96 horas, e os isolados de Aspergillus spp. utilizados foram resistentes ao iodóforo nas concentrações testadas. A amônia quaternária e a clorexidina mostraram-se eficazes contra os isolados de Aspergillus spp., com exceção de um A. fumigatus e um A. terreus. Com estes resultados indica-se a utilização da amônia quaternária e da clorexidina na prevenção da aspergilose, questionando-se o uso de iodóforos para este fim.


2015 ◽  
Vol 3 (3) ◽  
pp. 374-379 ◽  
Author(s):  
Neveen Helmy Abou El-Soud ◽  
Mohamed Deabes ◽  
Lamia Abou El-Kassem ◽  
Mona Khalil

BACKGROUND: The leaves of Ocimum basilicum L. (basil) are used in traditional cuisine as spices; its essential oil has found a wide application in perfumery, dental products as well as antifungal agents.AIM: To assess the chemical composition as well as the in vitro antifungal activity of O. basilicum L. essential oil against Aspergillus flavus fungal growth and aflatoxin B1 production.MATERIAL AND METHODS: The essential oil of O. basilicum was obtained by hydrodistillation and analysed using gas chromatography (GC) and GC coupled with mass spectrometry (GC/MS). The essential oil was tested for its effects on Aspergillus flavus (A. flavus) mycelial growth and aflatoxin B1 production in Yeast Extract Sucrose (YES) growth media. Aflatoxin B1 production was determined by high performance liquid chromatography (HPLC).RESULTS: Nineteen compounds, representing 96.7% of the total oil were identified. The main components were as follows: linalool (48.4%), 1,8-cineol (12.2%), eugenol (6.6%), methyl cinnamate (6.2%), α-cubebene (5.7%), caryophyllene (2.5%), β-ocimene (2.1%) and α-farnesene (2.0%).The tested oil showed significant antifungal activity that was dependent on the used oil concentration. The complete inhibition of A. flavus growth was observed at 1000 ppm oil concentration, while marked inhibition of aflatoxin B1 production was observed at all oil concentrations tested (500, 750 and 1000 ppm).CONCLUSION: These results confirm the antifungal activities of O. basilicum L. oil and its potential use to cure mycotic infections and act as pharmaceutical preservative against A. flavus growth and aflatoxin B1 production.


2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Nawal Abd El-Baky ◽  
Raoufa Ahmed Abdel Rahman ◽  
Mona Mohammed Sharaf ◽  
Amro Abd Al Fattah Amara

After introducing the idea of using concentrations equal to or less than the minimum inhibition concentration (MIC) of some active chemical compounds for evacuating microbial cells, different types of microbes were evacuated. The original protocol was given the name sponge-like protocol and then was reduced and modified from a microorganism to another to prepare microbial ghosts for various applications such as immunological applications, drug delivery, and isolation of DNA and protein. Fungal pathogens that infect plants critically affect cost effectiveness, quality, and quantity of their production. They kill plant cells and/or cause plant stress. Plant fungal infections can originate from many sources such as infected soil, seeds, or crop debris causing diseases and quality losses around the world with billions of US dollars annually as costs of the associated productivity loss. This study focused on the application of the sponge-like protocol in protecting in vitro tissue cultures of plants against fungal pathogens. This can be useful for research purposes or may be developed to be introduced in field applications. Aspergillus flavus and Aspergillus niger infection in tissue culture of jojoba (Simmondsia chinensis (Link) Schn.) was used as a model to establish the employment of this protocol to control plant fungal diseases. The best conditions for A. flavus and A. niger ghosts production previously mapped by randomization experimental design (reduced Plackett–Burman experimental design) were used to prepare fungal ghosts. SDS, NaOH, NaHCO3, and H2O2 were used in their MIC (+1 level) or minimum growth concentration (MGC, −1 level) according to the determined optimal experimental design. The release of both of DNA and protein from the fungal cells was evaluated spectrophotometrically at 260nm and 280nm, respectively, as an indicator for cell loss of their cytoplasm. Fungal ghost cells were also examined by transmission electron microscopy. After confirming the preparation of high-quality fungal ghost cells, the same conditions were mimicked to control plant fungal infection. Jojoba grown in tissue culture was sprayed with fungal cells (about 103 CFU) as a control experiment or fungal cells followed by treatment with solution (a) represents the fungal ghost cells formation calculated critical concentration (FGCCC) of SDS, NaOH, and NaHCO3 and then treatment with solution (b) represents H2O2 FGCCC. The plant was examined on day 0 (plant grown before any infection or infection followed by treatment), day 5 (plant at day 5 after infection or infection followed by treatment), and day 10 (plant at day 10 after infection or infection followed by treatment). We observed fungal growth in case of control experiments at days 5 and 10 on the tissue culture medium, as well as plant, and the absence of any fungal growth in case of plant treated with FGCCC even after day 10. We recommend using this FGCCC in the form of chemical spraying formulation to treat the plants aiming to control different plant fungal infections in in vitro tissue culture systems or applied in field.


2020 ◽  
Vol 25 (3) ◽  
pp. 18-29
Author(s):  
Mohammed Al-mohaidi ◽  
Abdul_ameer S. Saadon

       Samples were collected from the stores , fields and local markets of Diwaniya province for the purpose of study  from August / October 2019, the following fungi were isolated: Aspergillus niger, Aspergillus flavus, Aspergillus ochraceus, Aspergillus fumigatus, Penicillium natatum , Rhizopus stolanifer, Alternaria Alternata, Fusarium solani, Fusarium proliferatum, Fusarium sp. Tricoderma sp. . Some significant differences were observed in the frequency of fungi isolated from fodder grains , the  frequency percentage highest in the treatment of non-sterilized grains reach 15.23% for the A. niger fungus and in sterilized grain about 13.88% . A.niger diagnosed phenotypic using classification keys and molecular diagnosed with PCR technique (polymerase chain reaction) with sequencing analysis and phylogenic tree analysis for the purpose of comparing A.niger fungi isolates with some global strains . The effect of  p.ostreatus showed ability of this fungus to inhibit growth of A.niger fungus when the antagonism occur between two fungus  . Interference between p.ostreatus filtrate concentration  and calcium citrate effect in the radial growth of the this fungus showed a clear decrease in the level of colonial diameters , where the inhibition percent reached to 71.79±0.06% at concentration 30% .


2017 ◽  
Vol 27 (6) ◽  
pp. 24-28
Author(s):  
Diana Barragan Ferrer ◽  
Jesus Manuel Barragan Ferrer ◽  
Rugilė Drulytė

Prieskoniniai (aromatiniai) augalai yra svarbus natūralių medžiagų šaltinis, juose yra daug antioksidantų – polifenolių, kurie svarbūs žmogaus organizmui: neutralizuoja žalingas reaktyvias deguonies ir azoto formas, sustiprina ląstelės antioksidantinės apsaugos sistemas bei padeda atkurti pažeistas struktūras. Per paskutinį dešimtmetį populiarėja prieskoninių augalų naudojimas ne tik kulinarijoje, bet ir medicinoje - gydymo tikslais. Tačiau prieskoniniuose augaluose esantys natūralūs junginiai, kurie pasižymi antioksidacinėmis savybėmis, gali skirtis nuo daugelio faktorių. Užsienio mokslininkų darbuose pateikti rezultatai tai ir atspindi. Norint išsiaiškinti prieskonių naudingumą, svarbu palyginti skirtingų rūšių ir kilmių biologines ypatybes, nes joms įtakos gali turėti skirtingos klimato sąlygos, augimo vietovė, prieskonių džiovinimo/paruošimo būdas ir ekstrakcijos laikas. Šio tyrimo tikslas nustatyti skirtingų kilmingumų, kvapiojo baziliko (Ocimum basilicum L.), paprastojo raudonėlio (Origanum vulgare L.) ir kvapiojo rozmarino (Rosmarinus officinalis L.) kokybę pagal bendrą fenolinių junginių kiekį ir antiradikalinį aktyvumą. Tyrimui buvo pasirinkti trijų rūšių prieskoniai iš skirtingų šalių: paprastasis raudonėlis (Lietuva, Ispanija, Gruzija, Kipras), kvapusis bazilikas (Lietuva, Ispanija, Gruzija, Egiptas), kvapusis rozmarinas (Lietuva, Ispanija, Sirija, Marokas). Iš gautų rezultatų nustatyta, kad didžiausias fenolinių junginių kiekis yra kilusio iš Gruzijos paprastojo raudonėlio augale ir kvapiojo baziliko, kurio kilmės šalis yra Egiptas. Stipriausias antiradikalinis aktyvumas modelinėje DPPH sistemoje nustatytas paprastojo raudonėlio ekstrakte, o mažiausias kvapiojo baziliko, kurių kilmės šalis Lietuva.


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