scholarly journals SUITABILITY OF ELECTRICAL CONDUCTIVITY TESTING AND ACCELERATED AGING FOR EVALUATING THE VIGOR OFCITHAREXYLUM MYRIANTHUM CHAM. DIASPORES

FLORESTA ◽  
2019 ◽  
Vol 49 (4) ◽  
pp. 701
Author(s):  
Ana Paula Bazzanella ◽  
Gabriele Larissa Mathias ◽  
Henrique Roberto Gattermann Mittelstaedt ◽  
Maiara Iadwizak Ribeiro ◽  
Andréa Maria Teixeira Forte ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Accelerated Aging ◽  
Germination Test ◽  
High Demand ◽  
Tukey Test ◽  
Aging Period ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Randomized Experimental Design ◽  
Native Tree Species

The Atlantic Forest is one of the most threatened Brazilian biomes by anthropic action. Thus, the interest inrecovering degraded areas through the use of native tree species has increased, causing a high demand in the use of vigorous seeds. This research aimed at evaluating the suitabilityof the electrical conductivity and accelerated aging tests to evaluate the vigor of C. myrianthum diaspores. For the adequacy of the electrical conductivity test, a completely randomized experimental design (CRD) was used in a 2 x 3 x9 (number of diaspores x volume of water x imbibition time)at a constant temperature of 25 °C. The procedure for the accelerated aging test was conducted at 40 ºC for 0 (no aging), 24, 48 and 72 hours. ACRDwas also used for the accelerated aging test, totaling 4 treatments. The results were submitted to analysis of variance (ANOVA) and the means were compared by the Tukey test at 5% probability. It is concluded that the electrical conductivity test for C. myrianthum diaspores can be conducted with 25 diaspores soaked with 50 mL of deionized water for 96 hours, thus replacing the germination test with the advantage of being carried out in a shorter period of time. Moreover,the 24 and 48 hour accelerated aging period is adequate to discriminate the vigor of C. myrianthum diaspores, with GSI and MGT being the most sensitive variables.

scholarly journals Accelerated Aging Test to Determine the Vigor of Mungbean Seeds

2019 ◽  
Vol 11 (7) ◽  
pp. 42
Author(s):  
Luciana Maria da Silva ◽  
Anderson Dias Vaz de Souza ◽  
Rogério Lámim Silva Junior ◽  
Natália Arruda ◽  
Nei Peixoto ◽  
...  
Keyword(s):  
Seed Quality ◽  
Germination Rate ◽  
Accelerated Aging ◽  
Dry Weight ◽  
Precise Determination ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Seedling Length ◽  
Aging Test ◽  
Randomized Experimental Design

The use of good quality seeds is fundamental for proper establishment of a crop. In this way, for more precise determination of seed quality, vigor tests are performed in addition to the germination test. These tests enable the selection of the best lots for commercialization and planting. This study evaluates the effects of temperature and exposure times of the accelerated aging test for vigor classification of mungbean seed lots. Seeds of the mungbean cultivar Esmeralda were used, being obtained from four cultivated plots at the State University of Goiás (UEG), Ipameri Câmpus, in the 2013, 2015, 2016, and 2017 harvests. The lots were initially characterized using germination and vigor tests (first germination count, germination rate index, seedling length, and seedling fresh and dry weight). The accelerated aging test was conducted with a completely randomized experimental design, in a 2 × 4 factorial arrangement consisting of two temperatures (40 and 42 °C) and four times (24, 48, 72, and 96 hours), with four replicates of 50 seeds per lot. The lots showed significant differences in the germination test, first germination count, germination rate index, and seedling length. The accelerated aging test was efficient in classifying lots. Lot 3 obtained the best results, while lot 1 obtained the lowest ones. The combination of 42 °C temperature and 72 h of seed exposure to the accelerated aging test is the best to classify mungbean seed lots.

scholarly journals Tests for evaluating the physiological quality of pitaya seeds

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano
Keyword(s):  
Electrical Conductivity ◽  
Pearson Correlation ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Water Volume ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe Hylocereus undatus fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p < 0.05). The germination test for H. undatus seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.

scholarly journals Seed vigor of Piptadenia moniliformis Benth. subjected to thermal stress

2020 ◽  
Vol 42 ◽  
pp. e51287
Author(s):  
Joyce Naiara da Silva ◽  
Monalisa Alves Diniz da Silva
Keyword(s):  
Electrical Conductivity ◽  
Sulfuric Acid ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Saturated Solution ◽  
Aging Period ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality ◽  
Concentrated Sulfuric Acid

 The objective of this work is to evaluate the influence of accelerated aging test at 41ºC on the germination and seed vigor of Piptadenia moniliformis Benth. Two experiments were conducted. Experiment 1: seeds were submitted to dormancy overcoming treatments. They were immersed into sulfuric acid for 0 (intact seeds), 5, 10, 15, 20 and 25 minutes. Experiment 2: two methods were evaluated for the accelerated aging test at 41ºC, that is, the traditional method and the saturated NaCl solution method with exposure periods of 0, 24, 48, 72 and 96 hours. After each treatment, the seeds were subjected to electrical conductivity and germination tests. Seeds overcame their dormancy when immersed into concentrated sulfuric acid for 5, 10, 15, 20 or 25 minutes, but the germination was faster when immersed for 20 minutes. The different methods for the accelerated aging test, decreased the germination potential and seed vigor of P. moniliformis after 24 hours of exposure. Regarding the method used, the saturated solution provided, in general, the best results since the seed water content was lower, thus reducing the proliferation of fungi. The electrical conductivity test showed that, by increasing the aging period regardless of the method, there is an increase in seed deterioration, corroborating with germination results, which showed that the correlation was moderate and negative and indicated that the higher the conductivity, the lower the percentage and the germination speed index. The immersion of seeds of P. moniliformis into concentrated sulfuric acid for 20 minutes provides a fast and increased germination. The methods for accelerated aging at 41ºC, traditional and saturated solution, can be used as vigor tests to evaluate the physiological quality of seeds.

scholarly journals Tests for evaluating the physiological quality of pitaya seeds

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4047
Author(s):  
Thiago Alberto Ortiz ◽  
Aline Moritz ◽  
Lúcia Sadayo Assari Takahashi ◽  
Mariana Ragassi Urbano
Keyword(s):  
Electrical Conductivity ◽  
Pearson Correlation ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Water Volume ◽  
Germination Test ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

<p>Germination test is used to assess the physiological quality of seeds; however, since it is carried out under ideal conditions, this test has not been shown sufficient for this purpose. Instead, it is possible to use vigor tests, although the lack of standardized methodologies has reduced their applicability and reproducibility. Thus, this study aimed to develop methodologies for conducting tests of germination, accelerated aging, and electrical conductivity for the evaluation of the physiological quality of pitaya seeds. For this purpose, seeds from ripe <em>Hylocereus undatus </em>fruits were used. A completely randomized experimental design was used with four replications. The physiological quality of the seeds was assessed using germination, accelerated aging, and electrical conductivity tests, and the speed of germination index (SGI) and mean germination time (MGT) were determined for both the germination test and accelerated aging test. For the statistical analysis, we performed regression model adjustments and calculated the Pearson correlation coefficient (p &lt; 0.05). The germination test for <em>H. undatus </em>seeds can be performed at 25 °C, with the aim of reaching the highest SGI and lowest MGT values. The accelerated aging test can be conducted at 43 °C for 48 h, because combining these factors favors the expression of seed vigor, allowing seeds to achieve the maximum SGI and minimum MGT, while reducing the time of the assay. The electrical conductivity test can be performed using 25 seeds at a temperature of 30 °C and a water volume of 10 mL, since under these conditions there is less interference from external factors on the leachate content of the solution.</p>

scholarly journals ACCELERATED AGING AND ELECTRICAL CONDUCTIVITY TESTS IN CRAMBE SEEDS

2015 ◽  
Vol 39 (1) ◽  
pp. 7-14 ◽  
Author(s):  
Juliana Joice Pereira Lima ◽  
Marcella Nunes de Freitas ◽  
Renato Mendes Guimarães ◽  
Antônio Rodrigues Vieira ◽  
Maria Alice Bento Ávila
Keyword(s):  
Electrical Conductivity ◽  
Chloride Solution ◽  
Accelerated Aging ◽  
Germination Test ◽  
Crambe Seed ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

The aim of this study was to adapt the methodology of the accelerated aging and electrical conductivity tests for determination of physiological potential in crambe seeds. Six seed lots of crambe (cv. FMS Brilhante) were subjected to determination of moisture content, germination test, first count germination, emergence, and emergence speed index. For the accelerated aging test, the traditional methodology was used with water, and with a saturated potassium chloride and sodium chloride solution in three periods of exposure (24, 48, and 72 hours) at 41°C; the electrical conductivity test was performed with four pre-soaking treatments (0, 2, 4, and 8 hours) and four soaking periods (4, 8, 16, and 24 hours) at 25°C. The accelerated aging test with water for 72 hours and the electrical conductivity test with 2 hours of pre-soaking and assessment after 16 hours were effective for classification of the crambe seed lots in regard to physiological quality.

scholarly journals Electrical conductivity and accelerated aging in amaranth (Amaranthus crueentus L.) seeds

2018 ◽  
Vol 40 (1) ◽  
pp. 44-51
Author(s):  
Thaís D’Avila Rosa ◽  
Ariele Paula Nadal ◽  
Henrique Roberto Maldaner ◽  
Vanessa Nogueira Soares ◽  
Gizele Ingrid Gadotti ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Accelerated Aging ◽  
Water Volume ◽  
Amaranthus Cruentus ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Immersion Period ◽  
Amaranth Seed

Abstract: The objective of this study was to determine adequate conditions of water volume, seed quantity and immersion period to perform the electrical conductivity test, and to analyze the parameters of the accelerated aging test and its correlation with the the physiological potential of amaranth seeds. Five lots of amaranth seeds (Amaranthus cruentus), cultivar BRS Alegria, were used. The initial evaluation of the quality of these lots comprehended the following tests: water content, germination, first germination count, accelerated aging (traditional method, and modified versions, using unsaturated and saturated saline solution for the periods of 24, 48 and 72 h), electrical conductivity in different periods (2, 4, 6, 8 and 24 h), different quantities of water (25, 50 and 75 mL), different seed quantities (100 and 150), and field emergence. It could be concluded that the accelerated aging test at 41 °C, for 72 h, using unsaturated NaCl solution was efficient in the classification of amaranth seed lots in levels of vigor. The electrical conductivity test performed for 8 hours with 25 mL of water and 100 seeds was efficient in the classification of amaranth seeds as to their physiological potential.

scholarly journals Presence of glyphosate can harm the germination of bean seeds treated with biostimulant

2020 ◽  
Vol 36 (1) ◽  
Author(s):  
Jorgiani de Ávila ◽  
Fernanda Brito Cardoso ◽  
Sebastião Ferreira de Lima ◽  
Gustavo Ribeiro Barzotto ◽  
Mayara Santana Zanella
Keyword(s):  
Electrical Conductivity ◽  
Accelerated Aging ◽  
Cold Test ◽  
Seed Vigor ◽  
Common Beans ◽  
Accelerated Aging Test ◽  
Root And Shoot Length ◽  
Aging Test ◽  
Early Seedling ◽  
Different Doses

It is possible to cultivate common beans for a third harvest in one agricultural year due to varietal characteristics. For calendar adequacy, performing desiccation and planting often occur almost simultaneously. Germination performance of many plant species can improve with biostimulant use on seeds, however the interaction with herbicide residual molecules is unknown. The hypothesis is that seeds treated with a biostimulant in soil with glyphosate residues can eliminate the advantage of the biostimulant or increase the damage caused by the herbicide. The aim of this study was to evaluate the effect of different glyphosate reduce doses and the interaction with biostimulant on bean seed germination and vigor. The experiment was conducted using a completely randomized 2 x 5 factorial block designing factorial 2 x 5, corresponding to the presence and absence of biostimulant and five different doses of glyphosate reduce rates, with four repetitions. The conducted evaluations were first count germination, germination test, accelerated aging, cold test, root and shoot length, root and shoot dry matter and electrical conductivity. It can be concluded that the biostimulant treatment on bean seeds increased germination, seed vigor and early seedling growth, but glyphosate presence reduced those advantages, increasing electrical conductivity. However, the herbicide presence provided higher germination on the accelerated aging test.  

Reduction of the Forage Seeds Physiological Quality by the Contact with Fertilizer in the Crop-Livestock Integration

2019 ◽  
pp. 1-11
Author(s):  
Anne Caroline Dallabrida Avelino ◽  
Dayana Aparecida De Faria ◽  
Carlos Eduardo Avelino Cabral ◽  
Joadil Gonçalves De Abreu ◽  
Elisangela Clarete Camili ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Contact Time ◽  
Accelerated Aging ◽  
Germination Percentage ◽  
Randomized Design ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality ◽  
Completely Randomized Design ◽  
Seeds Germination

The aim of this study was to evaluate the effects of Brachiaria seed contact time with 05-25-15 fertilizer (N-P2O5-K2O) on the seed’s physiological quality. The experimental design was a completely randomized design with four replicates. Treatments were arranged in a 5x4 factorial, being: five seeds contact times with fertilizer (0, 24, 48, 72 and 96 hours); four forages: Marandu palisadegrass, Piatã palisadegrass, Xaraés palisadegrass and Ruziziensis grass. The performed tests were: water content, germination (first count and germination percentage), tetrazolium (viability), electrical conductivity, accelerated aging, sand emergency and emergence speed index. Contact time of 05-25-15 fertilizer with Brachiaria seeds reduces linearly: Germination, emergence and emergence speed index of Brachiaria seeds; Piatã and Xaraés palisadegrass seeds viability; vigor (electrical conductivity), except in Ruziziensis grass seeds; and seeds germination of Piatã palisadegrass submitted to accelerated aging test (vigor). Piatã palisadegrass is the least recommended, among the analyzed grasses, for intercropping in simultaneous sowing.

scholarly journals Improving the method for determining the physiological and sanitary potential of Gherkin seeds

2021 ◽  
Vol 37 ◽  
pp. e37087
Author(s):  
Hugo Cesar Rodrigues Moreira Catão ◽  
Ítala Menegon Castilho ◽  
Franciele Caixeta ◽  
Nilvanira Donizete Tebaldi ◽  
Pâmela Gomes Nakada Freitas
Keyword(s):  
Water Content ◽  
Saline Solution ◽  
Seed Quality ◽  
Accelerated Aging ◽  
Analysis Method ◽  
Germination Test ◽  
Seed Technology ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality

Gherkin seeds usually show irregular physiological quality. Seed production requires fast and reliable tests to evaluate seed quality. Germination test is considered a recognized analysis method; however, seed technology has pursuit the improvement of vigor tests aiming the evaluation of seed’s physiological potential. Thus, the objective of this work was to evaluate procedures to perform the test of accelerated aging and determine the physiological and sanitary potential of gherkin seeds. Four seed lots of cultivar Liso Calcuta were used in the study. To evaluate the initial physiological quality the water content was determined and germination and emergence tests, as well as indices of germination speed and emergence speed were used. The accelerated aging test was performed as traditionally and with saturated saline solution, with 48, 72 and 96 hours, at temperatures of 41oC and 45oC. After aging, the water content was determined, and seeds’ germination and sanity tests were performed. The experiment was set under a completely random design in factorial 4x3x2 (lots x aging periods x temperatures). The standard accelerated aging test and the test with saturated saline solution at 41oC for 96 hours were efficient to evaluate the vigor of gherkin seeds. Saturated saline solution provides uniform water absorption and deterioration in gherkin seeds, allowing to discriminate seed lots in different vigor levels. The salinity test after accelerated aging with saline solution reduces the incidence of some fungi.

scholarly journals Accelerated aging test in niger seeds

2015 ◽  
Vol 37 (3) ◽  
pp. 234-240 ◽  
Author(s):  
Carla Regina Baptista Gordin ◽  
Silvana de Paula Quintão Scalon ◽  
Tathiana Elisa Masetto
Keyword(s):  
Seedling Emergence ◽  
Accelerated Aging ◽  
Biodiesel Production ◽  
Germination Test ◽  
Decay Period ◽  
Content Variation ◽  
Randomized Design ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Completely Randomized Design

Abstract:Niger is a promising oilseed species for biodiesel production but there is no much information about the physiological potential of its seeds. Thus, the aim was to adapt the methodologies of accelerated aging test on six lots of niger seeds. The test was carried out by traditional and with saturated salt solution (20 and 40 g NaCl.100 mL-1) methods at 41 and 45 °C for 24, 48, 72 and 96 hours. After the decay period, the seeds were submitted to the germination test, proceeding to an evaluation on the seventh day after sowing, counting the normal seedlings percentage. A completely randomized design with four replications of 50 seeds was used and the means were compared by Tukey's test. The accelerated aging test was correlated with seedling emergence and provided lots classification in at least two levels of vigor. For the accelerated aging test, the method with 20 g NaCl.100 mL-1 at 41 °C for 24 hours is recommend. The traditional method is not suitable because it provides water content variation between samples above what is tolerable.

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